Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,323,749	T→C	G101G (GGT→GGC)	atoD →	acetyl‑CoA:acetoacetyl‑CoA transferase subunit alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,323,749	0	T	C	100.0%	121.7 / NA	35	G101G (GGT→GGC)	atoD	acetyl‑CoA:acetoacetyl‑CoA transferase subunit alpha
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (17/18);  total (17/18)

TGTTGATACCGGCATCGGTCCGCTCATCGTCAATGGTCGAGTCCGCAAAGTGATTGCTTCACATATCGGCACCAACCCGGAAACAGGTCGGCGCATGATATCTGGTGAGATGGACGTCGTTCTGGTGCCGCAAGGTACGCTAATCGAGCAAATTCGCTGTGGTGGAGCTGGACTTGGTGGTTTTCTCACCCCAACGGGTGTCGGCACCGTCGTAGAGGAAGGCAAACAGACACTGACACTCGACGGTAAAACCTGGCTGCTCGAACGC  >  NC_000913/2323614‑2323881                                                                                                                                        |                                                                                                                                     tgttgATACCGGCATCGGTCCGCTCATCGTCAATGGTCGAGTCCGCAAAGTGATTGCTTCACATATCGGCACCAACCCGGAAACAGGTCGGCGCATGATATCTGGTGAGATGGACGTCGTTCTGGTGCCGCAAGGCACGc                                                                                                                                  >  1:122343/1‑140 (MQ=255) tgttgATACCGGCATCGGTCCGCTCATCGTCAATGGTCGAGTCCGCAAAGTGATTGCTTCACATATCGGCACCAACCCGGAAACAGGTCGGCGCATGATATCTGGTGAGATGGACGTCGTTCTGGTGCCGCAAGGCACGc                                                                                                                                  >  2:138528/1‑140 (MQ=255)              aTCGGTCCGCTCATCGTCAATGGTCGAGTCCGCAAAGTGATTGCTTCACATATCGGCACCAACCCGGAAACAGGTCGGCGCATGATATCTGGTGAGATGGACGTCGTTCTGGTGCCGCAAGGCACGCTAATCGAGCAAAt                                                                                                                     <  1:93613/140‑1 (MQ=255)                 ggTACGCTCATCGTCAATGGTCAAGTCCGGAAAGTGACTGCTTCACATATCGGCACCAACCCGGAAACAGGTCGGCGCATGATATCTGGTGAGATGGACGTCGTTCTGGTGCCGCAAGGCACGCTAATCGAGCAAATTCg                                                                                                                  <  1:303404/140‑1 (MQ=255)                  gTCCGCTCATCGTCAATGGTCGAGTCCGCAAAGTGATTGCTTCACATATCGGCACCAACCCGGAAACAGGTCGGCGCATGATATCTGGTGAGATGGACGTCGTTCTGGTGCCGCAAGGCACGCTAATCGAGCAAATTCGc                                                                                                                 >  1:136296/1‑140 (MQ=255)                       cTCATCGTCAATGGTCGAGTCCGCAAAGTGATTGCTTCACATATCGGCACCAACCCGGAAACAGGTCGGCGCATGATATCTGGTGAGATGGACGTCGTTCTGGTGCCGCAAGGCACGCTAATCGAGCAAATTCGCTgtgg                                                                                                            <  1:509129/140‑1 (MQ=255)                            cGTCAATGGTCGAGTCCGCAAAGTGATTGCTTCACATATCGGCACCAACCCGGAAACAGGTCGGCGCATGATATCTGGTGAGATGGACGTCGTTCTGGTGCCGCAAGGCACGCTAATCGAGCAAATTCGCTGTGGTGGAg                                                                                                       <  2:485131/140‑1 (MQ=255)                             gTCAATGGTCGAGTCCGCAAAGTGATTGCTTCACATATCGGCACCAACCCGGAAACAGGTCGGCGCATGATATCTGGTGAGATGGACGTCGTTCTGGTGCCGGAAGGCACGCTAGTCCAGCAAACTCGCTGTGGTGGAGc                                                                                                      >  1:259613/1‑140 (MQ=255)                              tCAATGGTCGAGTCCGCAAAGTGATTGCTTCACATATCGGCACCAACCCGGAAACAGGTCGGCGCATGATATCTGGTGAGATGGACGTCGTTCTGGTGCCGCAAGGCACGCTAATCGAGCAAATTCGCTGTGGTGGAGCt                                                                                                     <  2:573753/140‑1 (MQ=255)                               cAATGGTCGAGTCCGCAAAGTGATTGCTTCACATATCGGCACCAACCCGGAAACAGGTCGGCGCATGATATCTGGTGAGATGGACGTCGTTCTGGTGCCGCAAGGCACGCTAATCGAGCAAATTCGCTGTGGTGGAGCTg                                                                                                    <  1:618889/140‑1 (MQ=255)                                        aGTCCGCAAAGTGATTGCTTCACATATCGGCACCAACCCGGAAACAGGTCGGCGCATGATATCTGGTGAGATGGACGTCGTTCTGGTGCCGCAAGGCACGCTAATCGAGCAAATTCGCTGTGGTGGAGCTGGACTtggtg                                                                                           <  2:566334/140‑1 (MQ=255)                                         gTCCGCAAAGTGATTGCTTCACATATCGGCACCAACCCGGAAACAGGTCGGCGCATGATATCTGGTGAGATGGACGTCGTTCTGGTGCCGCAAGGCACGCTAATCGAGCAAATTCGCTGTGGTGGAGCTGGACTtggtgg                                                                                          >  1:69364/1‑140 (MQ=255)                                          tCCGCAAAGTGATTGCTTCACATATCGGCACCAACCCGGAAACAGGTCGGCGCATGATATCTGGTGAGATGGACGTCGTTCTGGTGCCGCAAGGCACGCTAATCGAGCAAATTCGCTGTGGTGGAGCTGGACTTggtggt                                                                                         <  1:138528/140‑1 (MQ=255)                                            cGCAAAGTGATTGCTTCACATATCGGCACCAACCCGGAAACAGGTCGGCGCATGATATCTGGTGAGATGGACGTCGTTCTGGTGCCGCAAGGCACGCTAATCGAGCAAATTCGCTGTGGTGGAGCTGGACt                                                                                                <  2:87476/131‑1 (MQ=255)                                            cGCAAAGTGATTGCTTCACATATCGGCACCAACCCGGAAACAGGTCGGCGCATGATATCTGGTGAGATGGACGTCGTTCTGGTGCCGCAAGGCACGCTAATCGAGCAAATTCGCTGTGGTGGAGCTGGACt                                                                                                >  1:87476/1‑131 (MQ=255)                                                aaGTGATTGCTTCACATATCGGCACCAACCCGGAAACAGGTCGGCGCATGATATCTGGTGAGATGGACGTCGTTCTGGTGCCGCAAGGCACGCTAATCGAGCAAATTCGCTGTGGTGGAGCTGGACTTGGTGGTTTtctc                                                                                   <  2:16514/140‑1 (MQ=255)                                                             acaTATCGGCACCAACCCGGAAACAGGTCGGCGCATGATATCTGGTGAGATGGACGTCGTTCTGGTGCCGCAAGGCACGCTAATCGAGCAAATTCGCTGTGGTGGAGCTGGACTTGGTGGTTTTCTCACCCCAACGGgtg                                                                      >  1:560364/1‑140 (MQ=255)                                                               atatCGGCACCAACCCGGAAACAGGTCGGCGCATGATATCTGGTGAGATGGACGTCGTTCTGGTGCCGCAAGGCACGCTAATCGAGCAAATTCGCTGTGGTGGAGCTGGACTTGGTGGTTTTCTCACCCCAACGGGTGTc                                                                    >  1:162540/1‑140 (MQ=255)                                                                   cGGCACCAACCCGGAAACAGGTCGGCGCATGATATCTGGTGAGATGGACGTCGTTCTGGTGCCGCAAGGCACGCTAATCGAGCAAATTCGCTGTGGTGGAGCTGGACTTGGTGGTTTTCTCACCCCAACGGGTGTCGGCa                                                                >  2:342710/1‑140 (MQ=255)                                                                   cGGCACCAACCCGGAAACAGGTCGGCGCATGATATCTGGTGAGATGGACGTCGTTCTGGTGCCGCAAGGCACGCTAATCGAGCAAATTCGCTGTGGTGGAGCTGGACTTGGTGGTTTTCTCACCCCAACGGGTGTCGGCa                                                                <  2:659997/140‑1 (MQ=255)                                                                   cGGCACCAACCCGGAAACAGGTCGGCGCATGATATCTGGTGAGATGGACGTCGTTCTGGTGCCGCAAGGCACGCTAATCGAGCAAATTCGCTGTGGTGGAGCTGGACTTGGTGGTTTTCTCACCCCAACGGGTGTCGGCa                                                                >  1:613768/1‑140 (MQ=255)                                                                    ggCACCAACCCGGAAACCGGTCGGCGCATGACATCAGGTCAGATGGACATCGTTCTGGTGCCGCAAGGCACGCTAATCGAGCAAATTCGCTGTGGTGGAGCTGGACTTGGTGGTTTTCTCACCCCAACGGGTGTCGGCAc                                                               <  2:259613/140‑1 (MQ=255)                                                                    ggCACCAACCCGGAAACAGGTCGGCGCATGATATCTGGTGAGATGGACGTCGTTCTGGTGCCGCAAGGCACGCTAATCGAGCAAATTCGCTGTGGTGGAGCTGGACTTGGTGGTTTTCTCACCCCAACGGGTGTCGGCAc                                                               <  1:432647/140‑1 (MQ=255)                                                                           aCCCGGAAACAGGTCGGCGCATGATATCTGGTGAGATGGACGTCGTTCTGGTGCCGCAAGGCACGCTAATCGAGCAAATTCGCTGTGGTGGTGCTGGACTTGGTGGTTGTCTCACCCAAACGCGTGTCGGCACCGTCGTa                                                        >  2:267769/1‑140 (MQ=255)                                                                                   aCAGGTCGGCGCATGATATCTGGTGAGATGGACGTCGTTCTGGTGCCGCAAGGCACGCTAATCGAGCAAATTCGCTGTGGTGGAGCTGGACTTGGTGGTTTTCTCACCCCAACGGGTGTCGGCACCGTCGTAGAGGAAgg                                                <  2:162540/140‑1 (MQ=255)                                                                                       gTCGGCGCATGATATCTGGTGAGATGGACGTCGTTCTGGTGCCGCAAGGCACGCTAATCGAGCAAATTCGCTGTGGTGGAGCTGGACTTGGTGGTTTTCTCACCCCAACGGGTGTCGGCACCGTCGTAGAGGAAGGCaaa                                            >  2:562208/1‑140 (MQ=255)                                                                                       gTCGGCGCATGATATCTGGTGAGATGGACGTCGTTCTGGTGCCGCAAGGCACGCTAATCGAGCAAATTCGCTGTGGTGGAGCTGGACTTGGTGGTTTTCTCACCCCAACGGGTGTCGGCACCGTCGTAGAGGAAGGCaaa                                            <  1:371431/140‑1 (MQ=255)                                                                                                     tCTGGTGAGATGGACGTCGTTCTGGTGCCGCAAGGCACGCTAATCGAGCAAATTCGCTGTGGTGGAGCTGGACTTGGTGGTTTTCTCACCCCAACGGGTGTCGGCACCGTCGTAGAGGAAGGCAAACAgacactgacact                              <  2:523160/140‑1 (MQ=255)                                                                                                      cTGGGGAGATGGACGTCGTTCTGGTGCCGCAAGGCACGCTAAACGAGCAAATTCGCTGTGGTGGAGCTGGACTTGGTGGTTTTCTCACCCCAACGGGTGTCGGCACCGTCGTAGAGGAAGGCAAACAGACACTGACACTc                             <  2:449905/140‑1 (MQ=255)                                                                                                         gTGAGATGGACGTCGTTCTGGTGCCGCAAGGCACGCTAATCGAGCAAATTCGCTGTGGTGGAGCTGGACTTGGTGGTTTTCTCACCCCAACGGGTGTCGGCACCGTCGTAGAGGAAGGCAAACAGACACTGACACTCGAc                          >  1:45972/1‑140 (MQ=255)                                                                                                            agaTGGACGTCGTTCTGGTGCCGCAAGGCACGCTAATCGAGCAAATTCGCTGTGGTGGAGCTGGACTTGGTGGTTTTCTCACCCCAACGGGTGTCGGCACCGTCGTAGAGGAAGGCAAACAGACACTGACACTCGACGGt                       >  1:625247/1‑140 (MQ=255)                                                                                                             gaTGGACGTCGTTCTGGTGCCGCAAGGCACGCTAATCGAGCAAATTCGCTGTGGTGGAGCTGGACTTGGTGGTTTTCTCACCCCAACGGGTGTCGGCACCGTCGTAGAGGAAGGCAAACAGACACTGACACTCGACGGTa                      <  2:136296/140‑1 (MQ=255)                                                                                                             gaTGGACGTCGTTCTGGTGCCGCAAGGCACGCTAATCGAGCAAATTCGCTGTGGTGGAGCTGGACTTGGTGGTTTTCTCACCCCAACGGGTGTCGGCACCGTCGTAGAGGAAGGCAAACAGACACTGACACTCGACGGTa                      >  2:351465/1‑140 (MQ=255)                                                                                                                                ccGTAAGGCACGCTAATCGAGCAAATTCGCTGTGGTGGAGCTGGGCTTGGTGGTTTTCTCACCCCAACGGGTGTCGGCACCGTCGTAGAGGAAGGCAAACAGACACTGACACTCGACGGTAAAACCTGGCTGCTCGAACg   >  2:156437/1‑140 (MQ=255)                                                                                                                                ccGCAAGGCACGCTAATCGAGCAAATTCGCTGTGGTGGAGCTGGACTTGGTGGTTTTCTCACCCCAACGGGTGTCGGCACCGTCGTAGAGGAAGGCAAACAGACACTGACACTCGACGGTAAAACCTGGCTGCTCGAACg   >  2:131727/1‑140 (MQ=255)                                                                                                                                 cGCAAGGCACGCTAATCGAGCAAATTCGCTGTGGTGGAGCTGGACTTGGTGGTTTTCTCACCCCAACGGGTGTCGGCACCGTCGTAGAGGAAGGCAAACAGACACTGACACTCGACGGTAAAACCTGGCTGCTCGAACGc  <  2:69364/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                     TGTTGATACCGGCATCGGTCCGCTCATCGTCAATGGTCGAGTCCGCAAAGTGATTGCTTCACATATCGGCACCAACCCGGAAACAGGTCGGCGCATGATATCTGGTGAGATGGACGTCGTTCTGGTGCCGCAAGGTACGCTAATCGAGCAAATTCGCTGTGGTGGAGCTGGACTTGGTGGTTTTCTCACCCCAACGGGTGTCGGCACCGTCGTAGAGGAAGGCAAACAGACACTGACACTCGACGGTAAAACCTGGCTGCTCGAACGC  >  NC_000913/2323614‑2323881

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑