Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,326,589	A→G	T161A (ACC→GCC)	atoB →	acetyl‑CoA acetyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,326,589	0	A	G	100.0%	131.9 / NA	38	T161A (ACC→GCC)	atoB	acetyl‑CoA acetyltransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (22/16);  total (22/16)

GAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGCCACCCATGGTTATCATATGGGGATTACCGCCGAAAACGTGGCTAAAGAGTACGGAATTACCCGTGAAATGCAGGATGAACTGGCGCTACATTCACAGCGTAAAGCGGCAGCCGCAATTGAGTCCGGTGCTTTTACAGCCGAAATCGTCCCGGTAAATGT  >  NC_000913/2326462‑2326722                                                                                                                                |                                                                                                                                      gAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGCCACCCATGGTTATCATATGGGGATTGCCGCCGAAAACg                                                                                                                           <  1:111267/140‑1 (MQ=255)    tttAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGCCACCCATGGTTATCATATGGGGATTGCCGCCGAAAACGTgg                                                                                                                        >  1:152645/1‑140 (MQ=255)             cTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGCCACCCATGGTTATCATATGGGGATTGCCGCCGAAAACGTGGCTAAAGAGt                                                                                                               <  1:63205/140‑1 (MQ=255)               acttactCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGCCACCCATGGTTATCATATGGGGATTGCCGCCGAAAACGTGGCTAAAGAGTAc                                                                                                             <  1:568125/140‑1 (MQ=255)                        aTGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGCCACCCATGGTTATCATATGGGGATTGCCGCCGAAAACGTGGCTAAAGAGTACGGAATTAcc                                                                                                    >  2:624134/1‑140 (MQ=255)                        aTGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGCCACCCATGGTTATCATATGGGGATTGCCGCCGAAAACGTGGCTAAAGAGTACGGAATTAcc                                                                                                    >  2:352548/1‑140 (MQ=255)                        aTGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGCCACCCATGGTTATCATATGGGGATTGCCGCCGAAAACGTGGCTAAAGAGTACGGAATTAcc                                                                                                    >  2:498453/1‑140 (MQ=255)                         tGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGCCACCCATGGTTATTATATGGGGATTGCCCCAGAAAACGTGGCTAAAGAGTACGGAATTAccc                                                                                                   >  2:322951/1‑140 (MQ=255)                         tGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGCCACCCATGGTTATCATATGGGGATTGCCGCCGAAAACGTGGCTAAAGAGTACGGAATTAc                                                                                                     >  2:621875/1‑138 (MQ=255)                         tGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGCCACCCATGGTTATCATATGGGGATTGCCGCCGAAAACGTGGCTAAAGAGTACGGAATTAc                                                                                                     <  1:621875/138‑1 (MQ=255)                          gCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGCCACCCATGGTTATCATATGGGGATTGCCGCCGAAAACGTGGCTAAAGAGTACGGAATTACCCg                                                                                                  >  2:150554/1‑140 (MQ=255)                          gCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGCCACCCATGGTTATCATATGGGGATTGCCGCCGAAAACGTGGCTAAAGAGTACGGAATTACCCg                                                                                                  >  1:160730/1‑140 (MQ=255)                            aaaaGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGCCACCCATGGTTATCATATGGGGATTGCCGCCGAAAACGTGGCTAAAGAGTACGGAATTACCCGTg                                                                                                <  1:489957/140‑1 (MQ=255)                                                gTCTTGGAGACGGACAGGTTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGCCACCCATGGTTATCATATGGGGATTGCCGCCGAAAACGTGGCTAAAGAGTACGGAATTACCCGTGAAATGCAGGATGAACTGgcg                                                                            >  1:468766/1‑140 (MQ=255)                                                  cTTGGAGACGGACAGGTTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGCCACCCATGGTTATCATATGGGGATTGCCGCCGAAAACGTGGCTAAAGAGGACGGAATCACCGGTGAAATGCAGGATGAACTGGCGCt                                                                          >  1:241676/1‑140 (MQ=255)                                                    tGGAGACGGACAGGTTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGCCACCCATGGTTATCATATGGGGATTGCCGCCGAAAACGTGCCTAAAGAGTACGGAATTACCCGTGAAATGCAGGATGAACTGGCGCTAc                                                                        >  2:279266/1‑140 (MQ=255)                                                    tGGAGACGGACAGGTTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGCCACCCATGGTTATCATATGGGGATTGCCGCCGAAAACGTGACTAAAGAGAACGAAATAACCCGTGAAATGCAGGATGAACTGGCGCTAc                                                                        >  2:207526/1‑140 (MQ=255)                                                         aCGGACAGGTTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGCCACCCATGGTTATCATATGGGGATTGCCGCCGAAAACGTGGCTAAAGAGTACGGAATTACCCGTGAAATGCAGGATGAACTGGCGCTACATTca                                                                   <  2:607298/140‑1 (MQ=255)                                                           ggACAGGTTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGCCACCCATGGTTATCATATGGGGATTGCCGCCGAAAACGTGGCTAAAGAGTACGGAATTACCCGTGAAATGCAGGATGAACTGGCGCTACATTcaca                                                                 <  1:520560/140‑1 (MQ=255)                                                             aCAGGTTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGCCACCCATGGTTATCATATGGGGATTGCCGCCGAAAACGTGGCTAAAGAGTACGGAATTACCCGTGAAATGCAGGATGAACTGGCGCTACATTCACAGc                                                               >  1:621007/1‑140 (MQ=255)                                                                      tGACGTAATCCTGCGCGATGGCCTGATGTGCGCCACCCATGGTTATCATATGGGGATTGCCGCCGAAAACGTGGCTAAAGAGTACGGAATTACCCGTGAAATGCAGGATGAACTGGCGCTACATTCACAGCGTAAAGCgg                                                      <  2:557895/140‑1 (MQ=255)                                                                       gACGTAATCCTGCGCGATGGCCTGATGTGCGCCACCCATGGTTATCATATGGGGATTGCCGCCGAAAACGTGGCTAAAGAGTACGGAATTACCCGTGAAATGCAGGATGAACTGGCGCTACATTCACAGCGTAAAGCGGc                                                     <  2:617936/140‑1 (MQ=255)                                                                         cGTAATCCTGCGCGATGGCCTGATGTGCGCCACCCATGGTTATCATATGGGGATTGCCGCCGAAAACGTGGCTAAAGAGTACGGAATTACCCGTGAAATGCAGGATGAACTGGCGCTACATTCACAGCGTAAAGCGGCAg                                                   <  2:152645/140‑1 (MQ=255)                                                                           tAATCCTGCGCGATGGCCTGATGTGCGCCACCCATGGTTATCATATGGGGATTGCCGCCGAAAACGTGGCTAAAGAGTACGGAATTACCCGTGAAATGCAGGATGAACTGGCGCTACATTCACAGCGTAAAGCGGCAGcc                                                 >  2:640416/1‑140 (MQ=255)                                                                            aaTCCTGCGCGATGGCCTGATGTGCGCCACCCATGGTTATCATATGGGGATTGCCGCCGAAAACGTGGCTAAAGAGTACGGAATTACCCGTGAAATGCAGGATGAACTGGCGCTACATTCACAGCGTAAAGCGGCAGCCg                                                <  2:399431/140‑1 (MQ=255)                                                                                   cgcgATGGCCTGATGTGCGCCACCCATGGTTATCATATGGGGATTGCCGCCGAAAACGTGGCTAAAGAGTACGGAATTACCCGTGAAATGCAGGATGAACTGGCGCTACATTCACAGCGTAAAGCGGCAGCCGCAATTGa                                         <  2:160730/140‑1 (MQ=255)                                                                                        tGGCCTGATGTGCGCCACCCATGGTTATCATATGGGGATTGCCGCCGAAAACGTGGCTAAAGAGTACGGAATTACCCGTGAAATGCAGGATTAACTGGCGTTACGTTCTCAGCGTAAGGCGGGAGCCGCAATTGAGTCCg                                    >  2:176209/1‑140 (MQ=255)                                                                                                    cgcCACCCATGGTTATCATATGGGGATTGCCGCCGAAAACGTGGCTAAAGAGTACGGAATTACCCGTGAAATGCAGGATGAACTGGCGCTAGATTCACAGTGTACAGCCGCAGCCGCGATTGTGTCCGGTGCTTTTACAg                        >  2:202003/1‑140 (MQ=255)                                                                                                    cgcCACCCATGGTTATCATATGGGGATTGCCGCCGAAAACGTGGCTAAAGAGTACGGAATTACCCGTGAAATGCAGGATGAACTGGCGCTACATTCACAGCGTAAAGCGGCAGCCGCAATTGAGTCCGGTGCTTTTACAg                        >  1:608624/1‑140 (MQ=255)                                                                                                    cgcCACCCATGGTTATCATATGGGGATTGCCGCCGAAAACGTGGCTAAAGAGGACGGAATTACCCGTGACATGCAGGATGAACTGGCGCTACATTCACAGCGTAAAGCGGCAGCCGCAATTGAGTCCGGTGCTTTTACAg                        >  2:397606/1‑140 (MQ=255)                                                                                                     gcCACCCATGGTTATCATATGGGGATTGCCGCCGAAAACGTGGCTAAAGAGTACGGAATTACCCGTGAAATGCAGGATGAACTGGCGCTACATTCACAGCGTAAAGCGGCAGCCGCAATTGAGTCCGGTGCTTTTACAGc                       <  1:498453/140‑1 (MQ=255)                                                                                                      ccACCCATGGTTATCATATGGGGATTGCCGCCGAAAACGTGGCTAAAGAGTACGGAATTACCCGTGAAATGCAGGATGAACTGGCGCTACATTCACAGCGTACAGCGGCAGCCGAAATTGAGTCCGGTGCTTTTACAGcc                      >  1:267560/1‑140 (MQ=255)                                                                                                           cATGGTTATCATATGAGGATTGACGGCGATAACGTGGTTAAAGAGTACGGAATTACCCGTGAAATGCAGGATGAACTGGCGCTACATTCACAGCGTAAAGCGGCAGCCGCAATTGAGTCCGGTGCTTTTACAGCCGAAAt                 <  1:322951/140‑1 (MQ=255)                                                                                                              ggTTATCATATGGGGCTTGCCGTCGTAAATGTGGCTATAGAGTACGGAATTACCCGTGAAATGCAGGATGAACTGGCGCTACATTCACAGCGTAAAGCGGCAGCCGCAATTGAGTCCGGTGCTTTTACAGCCGAAATCGt              <  1:176209/140‑1 (MQ=255)                                                                                                               gTTATCATATGGGGATTGCCGCCGAAAACGTGGCTAAAGAGTACGGAATTACCCGTGAAATGCAGGATGAACTGGCGCTACATTCACAGCGTAAAGCGGCAGCCGCAATTGAGTCCGGTGCTTTTACAGCCGAAATCGTc             <  1:150554/140‑1 (MQ=255)                                                                                                                        tGGGGATTGCCGCCGAAAACGTGGCTAAAGAGTACGGAATTACCCGTGAAATGCAGGATGAACTGGCGCTACATTCACAGCGTAAAGCGGCAGCCGCAATTGAGTCCGGTGCTTTTACAGCCGAAATCGTCCCGGTAAAt    >  2:126214/1‑140 (MQ=255)                                                                                                                          gtgATTGCCGCCGAAAACGTGGCTAAAGGGTAGGGAATTACCCGGGAAATGCAGGATGAACTGGCGCTACATTCACAGCGTAAAGCGGCAGCCGCAGTTGAGTCCGGTGCTTTTACAGCCGAAATCGTCCCGGTAAAtgt  >  2:332595/3‑140 (MQ=255)                                                                                                                          gggATTGCCGCCGAAAACGTGGCTAAAGAGTACGGAATTACCCGTGAAATGCAGGATGAACTGGCGCTACATTCACAGCGTAAAGCGGCAGCCGCAATTGAGTCCGGTGCTTTTACAGCCGAAATCGTCCCGGTAAAtgt  >  1:77297/1‑140 (MQ=255)                                                                                                                                |                                                                                                                                      GAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGCCACCCATGGTTATCATATGGGGATTACCGCCGAAAACGTGGCTAAAGAGTACGGAATTACCCGTGAAATGCAGGATGAACTGGCGCTACATTCACAGCGTAAAGCGGCAGCCGCAATTGAGTCCGGTGCTTTTACAGCCGAAATCGTCCCGGTAAATGT  >  NC_000913/2326462‑2326722

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑