Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,342,454	T→C	T572T (ACA→ACG)	yfaL ←	putative autotransporter adhesin YfaL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,342,454	0	T	C	100.0%	143.3 / NA	43	T572T (ACA→ACG)	yfaL	putative autotransporter adhesin YfaL
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (17/26);  total (17/26)

ACGCGTGTCAACGTCGGAGGTAATCTCCATCACGCTTTCTTGTTGCTTATCGGTAAAGATAACCTGGCGGTTATAGTGGGTATCTCCAAGCTGCGAGTTGTCGCTCACCATCAGCGTACCGGAGGCGATTTGTGTT‑GTGCCCAAATAACTATTATTGTTTGCCAGACTTACCTGCCCGTCGCTCGCATCGACCACCACGTAGACGCCATCTTTCCCGTCGCCGCCGATATGCAAATTGTCGCCACCTGCAAGATCTACCACATCCTGAC  >  NC_000913/2342319‑2342587                                                                                                                                        |                                                                                                                                       aCGCGTGTCAACGTCGGAGGTAATCTCCATCACGCTTTCTTGTTGCTTATCGGTAAAGATAACCTGGCGGTTATAGTGGGTATCTCCAAGCTGCGAGTTGTCGCTCACCATCAGCGTACCGGAGGCGATTTGTGTC‑GTGc                                                                                                                                   >  2:463967/1‑140 (MQ=255)  cgcgTGTCAACGTCGGAGGTAATCTCCATCACGCTTTCTTGTTGCTTATCGGTAAAGATAACCTGGCGGTTATAGTGGGTATCTCCAAGCTGCGAGTTGTCGCTCACCATCAGCGTACCGGAGGCGATTTGTGTC‑GTGcc                                                                                                                                  <  1:557962/140‑1 (MQ=255)  cgcgTGTCAACGTCGGAGGTAATCTCCATCACGCTTTCTTGTTGCTTATCGGTAAAGATAACCTGGCGGTTATAGTGGGTATCTCCAAGCTGCGAGTTGTCGCTCACCATCAGCGTACCGGAGGCGATTTGTGTC‑GTGcc                                                                                                                                  >  2:532143/1‑140 (MQ=255)       gtCAACGTCGGAGGTAATCTCCATCACGCTTTCTTGTTGCTTATCGGTAAAGATAACCTGGCGGTTATAGTGGGTATCTCCAAGCTGCGAGTTGTCGCTCACCATCAGCGTACCGGAGGCGATTTGTGTC‑GTGCCCa                                                                                                                                >  2:483331/1‑137 (MQ=255)       gtCAACGTCGGAGGTAATCTCCATCACGCTTTCTTGTTGCTTATCGGTAAAGATAACCTGGCGGTTATAGTGGGTATCTCCAAGCTGCGAGTTGTCGCTCACCATCAGCGTACCGGAGGCGATTTGTGTC‑GTGCCCa                                                                                                                                <  1:483331/137‑1 (MQ=255)       gtCAACGTCGGAGGTAATCTCCATCACGCTTTCTTGTTGCTTATCGGTAAAGATAACCTGGCGGTTATAGTGGGTATCTCCAAGCTGCGAGTTGTCGCTCACCATCAGCGTACCGGAGGCGATTTGTGTC‑GTGCCCAAAt                                                                                                                             <  1:369372/140‑1 (MQ=255)       gtCAACGTCGGAGGTAATCTCCATCACGCTTTCTTGTTGCTTATCGGTAAAGATAACCTGGCGGTTATAGTGGGTATCTCCAAGCTGCGAGTTGTCGCTCACCATCAGCGTACCGGAGGCGATTTGTGTC‑GTGCCCAAAt                                                                                                                             <  1:463967/140‑1 (MQ=255)       gtCAACGTCGGAGGTAATCTCCATCACGCTTTCTTGTTGCTTATCGGTAAAGATAACCTGGCGGTTATAGTGGGTATCTCCAAGCTGCGAGTTGTCGCTCACCATCAGCGTACCGGAGGCGATTTGTGTC‑GTGCCCAAAt                                                                                                                             >  1:641815/1‑140 (MQ=255)             gTCGGAGGTAATCTCCATCACGCTTTCTTGTTGCTTATCGGTAAAGATAACCTGGCGGTTATAGTGGGTATCTCCAAGCTGCGAGTTGTCGCTCACCATCAGCGTACCGGAGGCGATTTGTGTC‑GTGCCCAAATAACtat                                                                                                                       <  1:532143/140‑1 (MQ=255)             gTCGGAGGTAATCTCCATCACGCTTTCTTGTTGCTTATCGGTAAAGATAACCTGGCGGTTATAGTGGGTATCTCCAAGCTGCGAGTTGTCGCACACCATCAGCGTACCGGAGGCGATTTGTGTC‑GTGCCCAAATAACtat                                                                                                                       <  2:93430/140‑1 (MQ=255)               cGGAGGTAATCTCCATCACGCTTTCTTGTTGCTTATCGGTAAAGATAACCTGGCGGTTATAGTGGGTATCTCCCAGCTGCGAGTTGTTGGTCACCATCAGCGTACCGGAGGCGATTT‑TGTTCGTGCCCAAATAACcattt                                                                                                                     >  2:369372/1‑139 (MQ=255)                      aaTCTCCATCACGCTTTCTTGTTGCTTATCGGTAAAGATAACCTGGCGGTTATAGTGGGTATCTCCAAGCTGCGAGTTGTCGCTCACCATCAGCGTACCGGAGGCGATTTGTGTC‑GTGCCCAAATAACTATTATTGTTTg                                                                                                              <  2:460999/140‑1 (MQ=255)                        tctcCATCACGCTTTCTTGTTGCTTATCGGTAAAGATAACCTGGCGGTTATAGTGGGTATCTCCAAGCTGCGAGTTGTCGCTCACCATCAGCGTACCGGAGGCGATTTGTGTC‑GTGCCCAAATAACTATTATTGTTTGcc                                                                                                            >  2:447546/1‑140 (MQ=255)                             aTCACGCTTTCTTGTTGCTTATCGGTAAAGATAACCAGGCGGTTATAGTGGGTATCTCCAAGCTGCGAGTTGTCGCTCACCATCAGCGTACGGGAGGCGACTTGGGTC‑GTGCCCAAAGAACTTTTATTGTTTGCCAGACt                                                                                                       >  2:251179/1‑140 (MQ=255)                              tCACGCTTTCTTGTTGCTTATCGGTAAAGATAACCTGGCGGTTATAGTGGGTATCTCCAAGCTGCGAGTTGTCGCTCACCATCAGCGTACCGGAGGCGATTTGTGTC‑GTGCCCAAATAACTATTATTGTTTGCCAGACtt                                                                                                      >  1:531939/1‑140 (MQ=255)                              tCACGCTTTCTTGTTGCTTATCGGTAAAGATAACCTGGCGGTTATAGTGGGTATCTCCAAGCTGCGAGTTGTCGCTCACCATCAGCGTACCGGAGGCGATTTGTGTC‑GTGCCCAAATAACTATTATTGTTTGCCAGACtt                                                                                                      >  1:92572/1‑140 (MQ=255)                               cACGCTTTCTTGTTGCTTATCGGTAAAGATAACCTGGCGGTTATAGTGGGTATCTTCAAGCTGCGAGTTGTCGCTCACCATCAGCGTACCGGAGGCGATTTGTGTC‑GTGCCCAAATAACTATTATTGTTTTCCAGACTTa                                                                                                     >  2:18260/1‑140 (MQ=255)                                 cGCTTTCTTGTTGCTTATCGGTAAAGATAACCTGGCGGTTATAGTGGGTATCTCCAAGCTGCGAGTTGTCGCTCACCATCAGCGTACCGGAGGCGATTTGTGTC‑GTGCCCAAATAACTATTATTGTTTGCCAGACTTAcc                                                                                                   >  1:399957/1‑140 (MQ=255)                                                tATCGGTAAAGATAACCTGGCGGTTATAGTGGGTATCTCCAAGCTGCGAGTTGTCGCTCACCATCAGCGTACCGGAGGCGATTTGTGTC‑GTGCCCAAATAACTATTATTGTTTGCCAGACTTACCTGCCCGTCGCTCGCa                                                                                    <  1:565393/140‑1 (MQ=255)                                                tATCGGTAAAGATAACCTGGCGGTTATAGTGGGTATCTCCAAGCTGCGAGTTGTCGCTCACCATCAGCGTACCGGAGGCGATTTGTGTC‑GTGCCCAAATAACTATTATTGTTTGCCAGACTTACCTGCCCGTCGCTCGCa                                                                                    <  2:442993/140‑1 (MQ=255)                                                        aaGATAACCTGGCGGTTATAGTGGGTATCTCCAAGCTGCGAGTTGTCGCTCACCATCAGCGTACCGGAGGCGATTTGTGTC‑GTGCCCAAATAACTATTATTGTTTGCCAGACTTACCTGCCCGTCGCTCGCATCGaccac                                                                            <  1:666947/140‑1 (MQ=255)                                                        aaGATAACCTGGCGGCTATAGTTGGCATCCCCAAGCTACGAGTTGTCGCTCACCATCAGCGTACCGGAGGCGATTTGTGTC‑GTGCCCAAATAACTATTATTGTTTGCCAGACTTACCTGCCCGTCGCTCGCATCGaccac                                                                            <  1:168389/140‑1 (MQ=255)                                                           aTAACCTGGCGGTTATAGTGGGTATCTCCAAGCTGCGAGATGTCGCTCACCATCAGCGTACCGGAGGCGATTTGTGTC‑GTGCCCAAATAACTATTATTGTTTGCCAGACTTACCTGCCCGTCGCTCGCATCGACcaccac                                                                         >  2:389357/1‑140 (MQ=255)                                                              aCCTGGCGGTTATAGTGGGTATCTCCAAGCTGCGAGTTGTCGCTCACCATCAGCGTACCGGAGGCGATTTGTGTC‑GTGCCCAAATAACTATTATTGTTTGCCAGACTTACCTGCCCGTCGCTCGCATCGACCACCACGTa                                                                      >  1:111860/1‑140 (MQ=255)                                                                cTGGCGGTTATAGTGGGTATCTCCAAGCTGCGAGTTGTCGCTCACCATCAGCGTACCGGAGGCGATTTGTGTC‑GTGCCCAAATAACTATTATTGTTTGCCAGACTTACCTGCCCGTCGCTCGCATCGACCACCACGTAGa                                                                    <  2:399957/140‑1 (MQ=255)                                                                    cGGTTATAGTGGGTACCTCCAACCTTCGACTTGTCGCTCACCATCAGCGTACCGGAGGCGATTTGTGTC‑GTGCCCAAATAACTATTATTGTTTGCCAGACTTACCTGCCCGTCGCTCGCATCGACCACCACGTAGACGcc                                                                <  1:251179/140‑1 (MQ=255)                                                                                 tATCTCCAAGCTGCGAGTTGTCGCTCACCATCAGCGTACCGGAGGCGATTTGTGTC‑GTGCCCAAATAACTATTATTGTTTGCCAGACTTACCTGCCCGTCGCTCGCATCGACCACCACGTAGACGCCATCTTTCCCGTcg                                                   >  2:62431/1‑140 (MQ=255)                                                                                  aTCTCCAAGCTGCGAGTTGTCGCTCACCATCAGCGTACCGGAGGCGATTTGTGTC‑GTGCCCAAATAACTATTATTGTTTGCCAGACTTACCTGCCCGTCGCTCGCATCGACCACCACGTAGACGCCATCTTTCCCGTcgc                                                  >  2:614006/1‑140 (MQ=255)                                                                                  aTCTCCAAGCTGCGAGTTGTCGCTCACCATCAGCGTACCGGAGGCGATTTGTGTC‑GTGCCCAAATAACTATTATTGTTTGCCAGACTTACCTGCCCGTCGCTCGCATCGACCACCACGTAGACGCCATCTTTCCCGTcgc                                                  >  2:48461/1‑140 (MQ=255)                                                                                     tcCAAGCTGCGAGTTGTCGCTCACCATCAGCGTACCGGAGGCGATTTGTGTC‑GTGCCCAAATAACTATTATTGTTTGCCAGACTTACCTGCACGTCGCTCACATCGACAACCACGTAAACGCCATCTTTCCCGTcgccgc                                               >  2:217438/1‑140 (MQ=255)                                                                                       cAAGCTGCGAGTTGTCGCTCACCATCAGCGTACCGGAGGCGATTTGTGTC‑GTGCCCAAATAACTATTATTGTTTGCCAGACTTACCTGCCCGTCGCTCGCATCGACCACCACGTAGACGCCATCTTTCCCGTCGccgccg                                             <  1:18260/140‑1 (MQ=255)                                                                                        aaGCTGCGAGTTGTCACTCACCCTCCGCGTACCGGAGACGATTTGTGTC‑GTGCCCAAATAACTATTATTGTTTGCCAGACTTACCTGCCCGTCGCTCGCATCGACCACCACGTAGACGCCATCTTTCCCGTCGCCGCCGa                                            <  1:190915/140‑1 (MQ=255)                                                                                              cGAGTTGTCGCTCACCATCAGCGTACCGGAGGCGATTTGTGTC‑GTGCCCAAATAACTATTATTGTTTGCCAGACTTACCTGCCCGTCGCTCGCATCGACCACCACGTAGACGCCATCTTTCCCGTCGCCGCCGATATGCa                                      >  1:97196/1‑140 (MQ=255)                                                                                                        cTCACCATCAGCGTACCGGAGGCGATTTGTGTC‑GTGCCCAAATAACTATTATTGTTTGCCAGACTTACCTGCCCGTCGCTCGCATCGACCACCACGTAGACGCCATCTTTCCCGTCGCCGCCGATATGCAAATTGTCGcc                            <  2:641815/140‑1 (MQ=255)                                                                                                        cTCACCATCAGCGTACCGGAGGCGATTTGTGTC‑GTGCCCAAATAACTATTATTGTTTGCCAGACTTACCTGCCCGTCGCTCGCATCGACCACCACGTAGACGCCATCTTTCCCGTCGCCGCCGATATGCAAATTGTCGcc                            <  1:614006/140‑1 (MQ=255)                                                                                                        cTCACCATCAGCGTACCGGAGGCGATTTGTGTC‑GTGCCCAAATAACTATTATTGTTTGCCAGACTTACCTGCCCGTCGCTCGCATCGACCACCACGTAGACGCCATCTTTCCCGTCGCCGCCGATATGCAAATTGTCGcc                            <  2:92572/140‑1 (MQ=255)                                                                                                         tCACCATCAGCGTACTGGAGGCCATTTGTGTC‑GTGCCTAAATAACTATTATTGTTTGCCAGACTTACCTGCCCGTCGCTCGCATCGACCACCACGTAGACGCCATCTTTCCCGTCGCCGCCGATATGCAAATTGTCGCCa                           <  1:305017/140‑1 (MQ=255)                                                                                                            ccATCAGCGTACCGGAGGCGATTTGTGTC‑GTGCCCAAATAACTATTATTGTTTGCCAGACTTACCTGCCCGTCGCTCGCATCGACCACCACGTAGACGCCATCTTTCCCGTCGCCGCCGATATACAAATTGTCGCCACCt                        <  1:484467/140‑1 (MQ=255)                                                                                                               tCAGCGTACCGGAGGCGATTTGTGTC‑GTGCCCAAATAACTATTATTGTTTGCCAGACTTACCTGCCCGTCGCTCGCATCGACCACCACGTAGACGCCATCTTTCCCGTCGCCGCCGATATGCAAATTGTCGCCACCTGCa                     <  1:102395/140‑1 (MQ=255)                                                                                                                   cGTACCGGAGGCGATTTGTGTC‑GTGCCCAAATAACTATTATTGTTTGCCAGACTTACCTGCCCGTCGCTCGCATCGACCACCACGTAGACGCCATCTTTCCCGTCGCCGCCGATATGCAAATTGTCGCCACCTGCAAGAt                 <  1:513181/140‑1 (MQ=255)                                                                                                                   cGTACCGGAGGCGATTTGTGTC‑GTGCCCAAATAACTATTATTGTTTGCCAGACTTACCTGCCCGTCGCTCGCATCGACCACCACGTAGACGACATCTTTCCCGTTGTCGCCGATATGCAAATGGTCGCCACCTGCAAGAt                 >  2:311363/1‑140 (MQ=255)                                                                                                                       ccGGAGGCGATTTGTGTC‑GTGCCCAAATAACTATTATTGTTTGCCAGACTTACCTGCCCGTCGCTCGCATCGACCACCACGTAGACGCCATCTTTCCCGTCGCCGCCGATATGCAAATTGTCGCCACCTGCAAGATCTAc             <  1:389357/140‑1 (MQ=255)                                                                                                                               gATTTGTGTC‑GTGCCCAAATAACTATTATTGTTTGCCAGACTTACCTGCCCGTCGCTCGCATCGACCACCACGTAGACGCCATCTTTCCCGTCGCCGCCGATATGCAAATTGTCGCCACCTGCAAGATCTACCACATCCt     <  2:111860/140‑1 (MQ=255)                                                                                                                                 tttGTGTC‑GTGCCCAAATAACTATTATTGTTTGCCAGACTTACCTGCCCGTCGCTCGCATCGACCACCACGTAGACGCCATCTTTCCCGTCGCCACCGATATGCAAATTGTCGCCACCTGCAAGATCTACCACATCCTGa   <  2:123792/140‑1 (MQ=255)                                                                                                                                 tttGTGTC‑GTGCCCAAATAACTATTATTGTTTCCCAGACTTCCCTGCCCGTCGCTCGCATCGACCACCACGTAGACGCCATCTTTCCCGTCGCCGCCGATATGCAAATTGTCGCCACCTGCAAGATCTACCACATCCTGa   <  2:122571/140‑1 (MQ=255)                                                                                                                                  ttgtgtc‑gtgCCCAAATAACTATTATTGTTTGCCAGACTTACCTGCCCGTCGCTCGCATCGACCACCACGTAGACGCCATCTTTCCCGTCGCCGCCGATATGCAAATTGTCGCCACCTGCAAGATCTACCACATCCTGAc  <  1:62431/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                       ACGCGTGTCAACGTCGGAGGTAATCTCCATCACGCTTTCTTGTTGCTTATCGGTAAAGATAACCTGGCGGTTATAGTGGGTATCTCCAAGCTGCGAGTTGTCGCTCACCATCAGCGTACCGGAGGCGATTTGTGTT‑GTGCCCAAATAACTATTATTGTTTGCCAGACTTACCTGCCCGTCGCTCGCATCGACCACCACGTAGACGCCATCTTTCCCGTCGCCGCCGATATGCAAATTGTCGCCACCTGCAAGATCTACCACATCCTGAC  >  NC_000913/2342319‑2342587

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑