Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,399,675	T→C	Y164C (TAC→TGC)	nuoG ←	NADH:quinone oxidoreductase subunit G

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,399,675	0	T	C	100.0%	128.9 / NA	37	Y164C (TAC→TGC)	nuoG	NADH:quinone oxidoreductase subunit G
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (16/21);  total (16/21)

TGTCGGTAAATACGCCGGTCGGGCAAATTTCGACCAGGTTACCGGAAAATTCGCTTTCCAGCGTGCCGTCTTCCGGGCGACCGAAGTAGACGTTGTCGTGCGCACCGTAAACGCCCAGATCTGTACCGTCAGCGTAATCTTTGTAGTAACGCACACAGCGGTAGCAGGCGATGCAGCGGTTCATTTCGTGAGAGATGAATGGCCCCAAATCCTGATTACGGTGGGTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCA  >  NC_000913/2399541‑2399810                                                                                                                                       |                                                                                                                                        tGTCGGTAAATACGCCGGTCGGTCAGATTTCGACCAGGTTACCGGAAAATTCGCTTTCCAGCGTGCCGTCTTCCGGGCGACCGAAGTAGACGTTGTCGTGCGCACCGTAAACGCCCAGATCTGTACCGTCAGCGCAATCt                                                                                                                                    <  1:219511/140‑1 (MQ=255)  gTCGGTAAATACGCCGGTCGGGCAAATTTCGACCAGGTTACCGGAAAATTCGCTTTCCAGCGTGCCGTCTCCCGGGCGACCGAAGTAGACGTTGTCGTGCGCACCGTAAACGCCCAGATCTGTACCGTCAGCGCAATCtt                                                                                                                                   <  2:495499/140‑1 (MQ=255)    cGGTAAATACGCCGGTCGGGCAAATTTCGACCAGGTTACCGGAAAATTCGCTTTCCAGCGTGCCGTCTTCCGGGCGACCGAAGTAGACGTTGTCGTGCGCTCCGTAAATGCCCAGATATGTAACGTCAGCGCAATCTTTg                                                                                                                                 >  2:275068/1‑140 (MQ=255)    cGGTAAATACGCCGGTCGGGCAAATTTCGACCAGGTTACCGGAAAATTCGCTTTCCAGCGTGCCGTCTTCCGGGCGACCGAAGTAGACGTTGTCGTGCGCACCGTAAACGCCCAGATCTGTACCGTCAGCGCAATCTTTg                                                                                                                                 <  1:497424/140‑1 (MQ=255)      gTAAATACGCCGGTCTGGCAAATTTAGACCAGGTTACCGGAAAATTCGCTTTCCAGCGTGCCGTCTTCCGGGCGACCGAAGTAGACGTTGTCGTGCGCACCGTAAACGCCCAGATCTGTACCGTCAGCGCAATCTTTgta                                                                                                                               <  1:280669/140‑1 (MQ=255)       tAAATACGCCGGTCGGGCAAATTTCGACCAGGTTACCGGAAAATTCGCTTTCCAGCGTGCCGTCTTCCGGGCGACCGAAGTAGACGTTGTCGTGCGCACCGTAAACGCCCAGATCTGTACCGTCAGCGCAATCTTTgtag                                                                                                                              <  1:143764/140‑1 (MQ=255)              gCCGGTCGGGCAAATTTCGACCAGGTTACCGGAAAATTCGCTTTCCAGCGTGCCGTCTTCCGGGCGACCGAAGTAGACGTTGTCGTGCGCACCGTAAACGCCCAGATCTGTACCGTCAGCGCAATCTTTGTAGTAACGca                                                                                                                       >  1:31378/1‑140 (MQ=255)                 ggTCGGGCAAATTTCGACCAGGTTACCGGAAAATTCGCTTTCCAGCGTGCCGTCTTCCGGGCGACCGAAGTAGACGTTGTCGTGCGCACCGGAAACGCCCCGATCTGTACCGTCAGCGCAATTTTTGTAGTAACGcacac                                                                                                                    >  2:282465/1‑140 (MQ=255)                   tCGGGCAAATTTCGACCAGGTTACCGGAAAATTCGCTTTCCAGCGTGCCGTCTTCCGGGCGACCGAAGTAGACGTTGTCGTGCGCACCGTAAACGCCCAGATCTGTACCGTCAGCGCAATCTTTGTAGTAACGCACACAg                                                                                                                  >  2:82825/1‑140 (MQ=255)                        cAAATTTCGACCAGGTTACCGGAAAATTCGCTTTCCAGCGTGCCGTCTTCCGGGCGACCGAAGTAGACGTTGTCGTGCGCACCGTAAACGCCCAGATCTGTACCGTCAGCGCAATCTTTGTAGTAACGCACACAGCGGTa                                                                                                             >  1:392522/1‑140 (MQ=255)                        cAAATTTCGACCAGGTTACCGGAAAATTCGCTTTCCAGCGTGCCGTCTTCCGGGCGACCGAAGTAGACGTTGTCGTGCGCACCGTAAACGCCCAGATCTGTACCGTCAGCGCAATCTTTGTAGTAACGCACACAGCGGTa                                                                                                             >  2:560981/1‑140 (MQ=255)                                    aGGTTACCGGAAAATTCGCTTTCCAGCGTGCCGTCTTCCGGGCGACCGAAGTAGACGTTGTCGTGCGCACCGTAAACGCCCAGATCTGTACCGTCAGCGCAATCTTTGTAGTAACGCACACAGCGGTAGCAGGCGATGCa                                                                                                 <  2:128850/140‑1 (MQ=255)                                    aGGTTACCGGAAAATTCGCTTTCCAGCGTGCCGTCTTCCGGGCGACCGAAGTAGACGTTGTCGTGCGCACCGTAAACGCCCAGATCTGTACCGTCAGCGCAATCTTTGTAGTAACGCACACAGCGGTAGCAGGCGATGCa                                                                                                 <  1:562155/140‑1 (MQ=255)                                    aGGTTACCGGAAAATGCGCTTTTCAGCGTTCCGTCTTTCGGGCGACCGAAGTAGACGTTGTCGTGCGCACCGTAAACGCCCAGATCTGTACCGTCAGCGCAATCTTTGTAGTAACGCACACAGCGGTAGCAGGCGATGCa                                                                                                 <  1:300915/140‑1 (MQ=255)                                       ttACCGGAAAATTCGCTTTCCAGCGTGCCGTCTTCCGGGCGACCGAAGTAGACGTTGTCGTGCGCACCGTAAACGCCCAGATCTGTACCGTCAGCGCAATCTTTGTAGTAACGCACACAGCGGTAGCAGGCGATGCAGCg                                                                                              <  2:77068/140‑1 (MQ=255)                                          ccGGAAAATTCGCTTTCCAGCGTGCCGTCTTCCGGGCGACCGAAGTAGACGTTGTCGTGCGCACCGTAAACGCCCAGATCTGTACCGTCAGCGCAATCTTTGTAGTAACGCACACAGCGGTAGCAGGCGATGCAGCGGtt                                                                                           <  1:560981/140‑1 (MQ=255)                                             gAAAATTCGCTTTCCAGCGTGCCGTCTTCCGGGCGACCGAAGTAGACGTTGTCGTGCGCACCGTAAACGCCCAGATCTGTACCGTCAGCGCAATCTTTGTAGTAACGCACACAGCGGTAGCAGGCGATGCAGCGGTTCAt                                                                                        <  1:82825/140‑1 (MQ=255)                                                       tttCCAGCGTGCCGTCTTCCGGGCGTCCGAAGTAGACGTTGTCGTGCGCACCGTAAACGCCCAGATCTGTACCGTCAGCGCAATCTTTGTAGTAACGCACACAGCGGTAGCAGGCGATGCAGCGGTTCATTTCGTgagag                                                                              <  1:282465/140‑1 (MQ=255)                                                        ttCCAGCGTGCCGTCTTCCGGGCGACCGAAGTAGACGTTGTCGTGCGCACCGTAAACGCCCAGATCTGTACCGTCAGCGCAATCTTTGTAGTAACGCACACAGCGGTAGCAGGCGATGCAGCGGTTCATTTCGTgagaga                                                                             >  2:569470/1‑140 (MQ=255)                                                              cGTGCCGTCTTCCGGTCGACCGCAGTAGAAGTTGTCGTGCGCACCGTAAACGCCCAGATCTGTACCGTCAGCGCAATCTTTGTAGTAACGCACACAGCGGTAGCAGGCGATGCAGCGGTTCATTTCGTGAGAGATGAATg                                                                       <  1:275068/140‑1 (MQ=255)                                                                   cGTCTTCCGGGCGACCGAAGTAGACGTTGTCGTGCGCACCGTAAACGCCCAGATCTGTACCGTCAGCGCAATCTTTGTAGTAACGCACACAGCGGTAGCAGGCGATGCAGCGGTTCATTTCGTGAGAGATGAATGGcccc                                                                  <  1:452147/140‑1 (MQ=255)                                                                   cGTCTTCCGGGCGACCG‑AGTAGACGTTGTCGTGCGCACCGTAAACGCCCAGATCTGTACCGTCAGCGCAATCTTTGTAGTAACGCACACAGCGGTAGCAGGCGATGCAGCGGTTCATTTCGTGAGAGATGAATGGCCCCa                                                                 <  2:359126/140‑1 (MQ=255)                                                                            ggCGACCGAAGTAGACGTTGTCGTGCGCACCGTAAACGCCCAGATCTGTACCGTCAGCGCAATCTTTGTAGTAACGCACACAGCGGTAGCAGGCGATGCAGCAGTTCATTTCGTGAGGGATGAAGGGCCCCAAATCCTGa                                                         >  1:178973/1‑140 (MQ=255)                                                                               gACCGAAGTAGACGTTGTCGTGCGCACCGTAAACGCCCAGATCTGTACCGTCAGCGCAATCTTTGTAGTAACGCACACAGCGGTAGCAGGCGATGCAGCGGTTCCTTTCGTGAGAGACGAATAGCCCCAAATCCTGATTa                                                      >  2:242378/1‑140 (MQ=255)                                                                                 ccGAAGTAGACGTTGTCGTGCGCCCCGTAAACGCCCAGATCTGTACCGTCAGCGCAATCTTTGTAGTAACGCACACAGCGGTAGCAGGCGATGCAGCGGTTCATTTCGTGAGAGATGAATGGCCCCAAATCCTGATTACg                                                    <  1:435657/140‑1 (MQ=255)                                                                                 ccGAAGTAGACGTTGTCGTGCGCACCGTAAACGCCCAGATCTGTACCGTCAGCGCAATCTTTGTAGTAACGCACACAGCGGTAGCAGGCGATGCAGCGGTTCATTTCGTGAGAGATGAATGGCCCCAAATCCTGATTACg                                                    <  1:25653/140‑1 (MQ=255)                                                                                 ccGAAGTAGACGTTGTCGTGCGAACCGTATACGCCCATATCTGTACCGTCAGCGCAATCTTTGTAGTAACGCACACAGCGGTAGCAGGCGATGCAGCGGTTCATTTCGTGAGAGATGAATGGCCCCAAATCCTGATTACg                                                    <  1:242378/140‑1 (MQ=255)                                                                                               gTCGTGCGCACCGTAAACGCCCAGATCTGTACCGTCAGCGCAATCTTTGTAGTAACGCACACAGCGGTAGCAGGCGATGCAGCGGTTCATTTCGTGAGAGATGAATGGCCCCAAATCCTGATTACGGTGGGTACGTTTgg                                      >  1:519252/1‑140 (MQ=255)                                                                                               gTCGTGCGCACCGTAAACGCCCAGATCTGTACCGTCAGCGCAATCTTTGTAGTAACGCACACAGCGGTAGCAGGCGATGCAGCGGTTCATTTCGTGAGAGATGAATGGCCCCAAATCCTGATTACGGTGGGTACGTTTgg                                      >  2:464686/1‑140 (MQ=255)                                                                                               gTCGTGCGCACCGTAAACGCCCAGATCTGTACCGTCAGCGCAATCTTTGTAGTAACGCACACAGCGGTAGCAGGCGATGCAGCGGTTCATTTCGTGAGAGATGAATGGCCCCAAATCCTGATTACGGTGGGTACGTTTgg                                      >  2:470993/1‑140 (MQ=255)                                                                                               gTCGTGCGCACCGTAAACGCCCAGATCTGTACCGTCAGCGCAATCTTTGTAGTAACGCACACAGCGGTAGCAGGCGATGCAGCGGTTCATTTCGTGAGAGATGAATGGCCCCAAATCCTGATTACGGTGGGTACGTTTgg                                      >  2:96860/1‑140 (MQ=255)                                                                                                                              ccGTCAGCGCAATCTTTGTAGTAACGCACACAGCGGTAGCAGGCGATGCAGCGGTTCATTTCGTGAGAGATGAATGGCCCCAAATCCTGATTACGGTGGGTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGt       >  1:397024/1‑140 (MQ=255)                                                                                                                                 tCAGCGCAATCTTTGTAGTAACGCACACAGCGGTAGCAGGCGATGCAGCGGTTCATTTCGTGAGAGATGAATGGCCCCAAATCCTGATTACGGTGGGTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGtcat    >  2:10548/1‑140 (MQ=255)                                                                                                                                 tCAGCGCAATCTTTGTAGTAACGCACACAGCGGTAGCAGGCGATGCAGCGGTTCATTTCGTGAGAGATGAATGGCCCCAAATCCTGATTACGGTGGGTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGt       <  2:468321/137‑1 (MQ=255)                                                                                                                                 tCAGCGCAATCTTTGTAGTAACGCACACAGCGGTAGCAGGCGATGCAGCGGTTCATTTCGTGAGAGATGAATGGCCCCAAATCCTGATTACGGTGGGTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGt       >  1:468321/1‑137 (MQ=255)                                                                                                                                   aGCGCAATCTTTGTAGTAACGCACACAGCGGTAGCAGGCGATGCAGCGGTTCATTTCGTGAGAGATGAATGGCCCCAAATCCTGATTACGGTGGGTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGtcatca  <  1:96860/140‑1 (MQ=255)                                                                                                                                   aGCGCAATCTTTGTAGTAACGCACACAGCGGTAGCAGGCGATGCAGCGGTTCATTTCGTGAGAGATGAATGGCCCCAAATCCTGATTACGGTGGGTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGtcatca  <  1:470993/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                        TGTCGGTAAATACGCCGGTCGGGCAAATTTCGACCAGGTTACCGGAAAATTCGCTTTCCAGCGTGCCGTCTTCCGGGCGACCGAAGTAGACGTTGTCGTGCGCACCGTAAACGCCCAGATCTGTACCGTCAGCGTAATCTTTGTAGTAACGCACACAGCGGTAGCAGGCGATGCAGCGGTTCATTTCGTGAGAGATGAATGGCCCCAAATCCTGATTACGGTGGGTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCA  >  NC_000913/2399541‑2399810

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑