Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,479,931	T→C	L244L (TTG→CTG)	dsdA →	D‑serine ammonia‑lyase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,479,931	0	T	C	100.0%	130.5 / NA	37	L244L (TTG→CTG)	dsdA	D‑serine ammonia‑lyase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (20/17);  total (20/17)

AGCGAAACTGCGCAGCCATGGCGTTACGGTCGTGGAATATGAGCAAGATTATGGTGTTGCCGTCGAGGAAGGACGTAAAGCAGCGCAGTCTGACCCGAACTGTTTCTTTATTGATGACGAAAATTCCCGCACGTTGTTCCTTGGGTATTCCGTCGCTGGCCAGCGTCTTAAAGCGCAATTTGCCCAGCAAGGCCGTATCGTCGATGCTGATAACCCTCTGTTTGTCTATCTGCCGTGTGGTGTTGGCGGTGGTCCTGGTGGCGTCGCA  >  NC_000913/2479798‑2480065                                                                                                                                      |                                                                                                                                       aGCGAAACTGCGCAGCCATGGCGTTACGGTCGTGGAATATGAGCAAGATTATGGTGTTGCCGTCGAGGAAGGACGTAAAGCAGCGCAGTCTGACCCGAACTGTTTCTTTATTGATGACGAAAATTCCCGCACGCTGTTcc                                                                                                                                  <  2:582805/140‑1 (MQ=255)           cgcAGCCATGGCGTTACGGTCGTGGAATATGAGCAAGATTATGGTGTTGCCGTCGAGGAAGGACGTAAAGCAGCGCAGTCTGACCCGAACTGTTTCTTTATTGATGACGAAAATTCCCGCACGCTGTTCCTTGGGTATTc                                                                                                                        >  1:137966/1‑140 (MQ=255)           cgcAGCCATGGCGTTACGGTCGTGGAATATGAGCAAGATTATGGTGTTGCCGTCGAGGAAGGACGTAAAGCAGCGCAGTCTGACCCGAACTGTTTCTTTATTGATGACGAAAATTCCCGCACGCTGTTCCTTGGGTATTc                                                                                                                        <  1:503894/140‑1 (MQ=255)                          aCGGTCGTGGAATATGAGCAAGATTATGGTGTTGCCGTCGAGGAAGGACGTAAAGCAGCGCAGTCTGACCCGAACTGTTTCTTTATTGATGACGAAAATTCCCGCACGCTGTTCCTTGGGTATTCCGTCGCTGGCCAGCg                                                                                                         >  2:490681/1‑140 (MQ=255)                                              aGATTATGGTGTTGCCGTCGAGGAAGGACGTAAAGCAGCGCAGTCTGACCCGAACTGTTTCTTTATTGATGACGAAAATTCCCGCACGCTGTTCCTTGGGTATTCCGTCGCTGGCCAGCGTCTTAAAGCGCAATTTGccc                                                                                     >  1:569266/1‑140 (MQ=255)                                                aTTATGGTGTTGCCGTCGAGGAAGGACGTAAAGCAGCGCAGTCTGACCCGAACTGTTTCTTTATTGATGACGAAAATTCCCGCACGCTGTTCCTTGGGTATTCCGTCGCTGGCCAGCGTCTTAAAGCGCAATTTGCCCAg                                                                                   <  1:660616/140‑1 (MQ=255)                                                 ttATGGTGTTGCCGTCGAGGAAGGACGTAAAGCAGCGCAGTCTGACCCGAACTGTTTCTTTATTGAGGACGCAAATTCCCGCACGCTGTTCCTTGGGTATTCCGTCGCTGGCCAGCGTCTTAAAGCGCAATTTGCCCAGc                                                                                  >  2:382615/1‑140 (MQ=255)                                                      gtgtTGCCGTCGAGGAAGGACGTAAAGCAGCGCAGTCTGACCCGAACTGTTTCTTTATTGATGACGAAAATTCCCGCACGCTGTTCCTTGGGTATTCCGTCGCTGGCCAGCGTCTTAAAGCGCAATTTGCCCAGCAAGGc                                                                             >  1:406895/1‑140 (MQ=255)                                                      gtgtTGCCGTCGAGGAAGGACGTAAAGCAGCGCAGTCTGACCCGAACTGTTTCTTTATTGATGACGAAAATTCCCGCACGCTGTTCCTTGGGTATTCCGTCGCTGGCCAGCGTCTTAAAGCGCAATTTGCCCAGCAAGGc                                                                             >  1:658847/1‑140 (MQ=255)                                                         ttGCCGTCGAGGAAGGACGTAAAGCAGCGCAGTCTGACCCGAACTGTTTCTTTATTGATGACGAAAATTCCCGCACGCTGTTCCTTGGGTATTCCGTCGCTGGCCAGCGTCTTAAAGCGCAATTTGCCCAGCAAGGCCGt                                                                          <  2:111290/140‑1 (MQ=255)                                                         ttGCCGTCGAGGAAGGACGTAAAGCAGCGCAGTCTGACCCGAACTGTTTCTTTATTGATGACGAAAATTCCCGCACGCTGTTCCTTGGGTATTCCGTCGCTGGCCAGCGTCTTAAAGCGCAATTTGCCCAGCAAGGCCGt                                                                          >  1:657132/1‑140 (MQ=255)                                                          tGCCGTCGAGGAAGGACGTAAAGCAGCGCAGTCTGACCCGAACTGTTTCTTTATTGATGACGAAAATTCCCGCACGCTGTTCCTTGGGTATTCCGTCGCTGGCCAGCGTCTTAAAGCGCAATTTGCCCAGCAAGGCCGTa                                                                         >  2:620593/1‑140 (MQ=255)                                                                  aggaaggaCGTAAAGCAGCGCAGTCTGACCCGAACTGTTTCTTTATTGATGACGAAAATTCCCGCACGCTGTTCCTTGGGTATTCCGTCGCTGGCCAGCGTCTTAAAGCGCAATTTGCCCAGCAAGGCCGTATCGTCGAt                                                                 <  2:627231/140‑1 (MQ=255)                                                                         aCGTAAAGCAGCGCAGTCTGACCCGAACTGTTTCTTTATTGATGACGAAAATTCCCGCACGCTGTTCCTTGGGTATTCCGTCGCTGGCCAGCGTCTTAAAGCGCAATTTGCCCAGCAAGGCCGTATCGTCGATGCTGATa                                                          <  2:376514/140‑1 (MQ=255)                                                                            tAAAGCAGCGCAGTCTGACCCGAACTGTTTCTTTATTGATGACGAAAATTCCCGCACGCTGTTCCTTGGGTATTCCGTCGCTGGCCAGCGTCTTAAAGCGCAATTTGCCCAGCAAGGCCGTATCGTCGATGCTGATAAcc                                                       <  1:620593/140‑1 (MQ=255)                                                                               agcagcGCAGTCTGACCCGAACTGTTTCTTTATTGATGACGAAAATTCCCGCACGCTGTTCCTTGGGTATTCCGTCGCTGGCCAGCGTCTTAAAGCGCAATTTGCCCAGCAAGGCCGTATCGTCGATGCTGATAACCctc                                                    <  2:530148/140‑1 (MQ=255)                                                                                 cagcGCAGTCTGACCCGAACTGTTTCTTTATTGATGACGAAAATTCCCGCACGCTGTTCCTTGGGTATTCCGTCGCTGGCCAGCGTCTTAAAGCGCAATTTGCCCAGCAAGGCCGTATCGTCGATGCTGATAACCCTCTg                                                  >  1:76944/1‑140 (MQ=255)                                                                                  agcGCAGTCTGACCCGAACTGTTTCTTTATTGATGACGAAAATTCCCGCACGCTGTTCCTTGGGTATTCCGTCGCTGGCCAGCGTCTTAAAGCGCAATTTGCCCAGCAAGGCCGTATCGTCGATGCTGAt                                                           <  2:545302/130‑1 (MQ=255)                                                                                  agcGCAGTCTGACCCGAACTGTTTCTTTATTGATGACGAAAATTCCCGCACGCTGTTCCTTGGGTATTCCGTCGCTGGCCAGCGTCTTAAAGCGCAATTTGCCCAGCAAGGCCGTATCGTCGATGCTGAt                                                           >  1:545302/1‑130 (MQ=255)                                                                                  agcGCAGTCTGACCCGAACTGTTTCTTTATTGATGACGAAAATTCCCGCACGCTGTTCCTTGGGTATTCCGTCGCTGGCCAGCGTCTTAAAGCGCAATTTGCCCAGCAAGGCCGTATCGTCGATGCTGATAACCCTCTGt                                                 >  1:330736/1‑140 (MQ=255)                                                                                   gcgcAGTCTGACCCGAACTGTTTCTTTATTGATGACGAAAATTCCCGCACGCTGTTCCTTGGGTATTCCGTCGCTGGCCAGCGTCTTAAAGCGCAATTTGCCCAGCAAGGCCGTATCGTCGATGCTGATAACCCTCTGtt                                                <  1:476070/140‑1 (MQ=255)                                                                                    cgcAGTCTGACCCGAACTGTTTCTTTATTGATGACGAAAATTCCCGCACGCTGTTCCTTGGGTATTCCGTCGCTGGCCAGCGTCTTAAAGCGCAATTTGCCCAGCAAGGCCGTATCGTCGATGCTGATAACCCTCTGttt                                               >  1:50075/1‑140 (MQ=255)                                                                                    cgcAGTCTGACCCGAACTGTTTATTTATTGAGGACAAAAATTCCCGCACGCTGTTCCTTGGGTATTCCGTCGCTGGCCAGCGTCTTAAAGCGCAATTTGCCCAGCAAGGCCGTATCGTCGATGCTGATAACCCTCTGttt                                               <  2:234492/140‑1 (MQ=255)                                                                                         tCTGACCCGAACTGTTTCTTTATTGATGACGAAAATTCCCGCACGCTGTTCCTTGGGTATTCCGTCGCTGGCCAGCGTCTTAAAGCGCAATTTGCCCAGCAAGGCCGTATCGTCGATGCTGATAAcc                                                       >  2:102430/1‑127 (MQ=255)                                                                                         tCTGACCCGAACTGTTTCTTTATTGATGACGAAAATTCCCGCACGCTGTTCCTTGGGTATTCCGTCGCTGGCCAGCGTCTTAAAGCGCAATTTGCCCAGCAAGGCCGTATCGTCGATGCTGATAAcc                                                       <  1:102430/127‑1 (MQ=255)                                                                                            gACCCGAACTGTTTCTTTATTGATGACGAAAATTCCCGCACGCTGTTCCTTGGGTATTCCGTCGCTGGCCAGCGTCTTAAAGCGCAATTTGCCCAGCAAGGCCGTATCGTCGATGCTGATAACCCTCTGTTTGTCTATCt                                       <  2:494786/140‑1 (MQ=255)                                                                                             aCCCGAACTGTTTCTTTATTGATGACGAAAATTCCCGCACGCTGTTCCTTGGGTATTCCGTCGCTGGCCAGCGTCTTAAAGCGCAATTTGCCCAGCAAGGCCGTATCGTCGATGCTGATAACCCTCTGTTTGTCTATCTg                                      <  1:490681/140‑1 (MQ=255)                                                                                               cctaaCTGTTTCTTTATTGATGACGAAAATTCCCGCACGCTGTTCCTTGGGTATTCCGTCGCTGGCCAGCGTCTTAAAGCGCAATTTGCCCAGCAAGGCCGTATCGTCGATGCTGATAACCCTCTGTTTGTCTATCTGcc                                    <  2:561725/137‑1 (MQ=255)                                                                                                  aaCTGTTTCTTTATTGATGACGAAAATTCCCGCACGCTGTTCCTTGGGTATTCCGTCGCTGGCCAGCGTCTTAAAGCGCAATTTGCCCAGCGAGGCCGTATCGTGGATCCTGATAACGCTCTGTTTGTCTATCTGCCgtg                                 >  2:185692/1‑140 (MQ=255)                                                                                                   aCTGTTTCTTTATTGATGACGAAAATTCCCGCACGCTGTTCCTTGGGTATTCCGTCGCTGGCCAGCGTCTTAAAGCGCAATTTGCCCAGCAAGGCCGTATCGTCGATGCTGATAACCCTCTGTTTGTCTATCTGCCgtgt                                >  1:661768/1‑140 (MQ=255)                                                                                                   aCTGTTTCTTTATTGATGACGAAAATTCCCGCACGCTGTTCCTTGGGTATTCCGTCGCTGGCCAGCGTCTTAAAGCGCAATTTGCCCAGCAAGGCCGTATCGTCGATGCTGATAACCCTCTGTTTGTCTATCTGCCgtgt                                >  1:448252/1‑140 (MQ=255)                                                                                                   aCTGTTTCTTTATTGATGACGAAAATTCCCGCACGCTGTTCCTTGGGTATTCCGTCGCTGGCCAGCGTCTTAAAGCGCAATTTGCCCAGCAAGGCCGTATCGTCGACGTTGATAACCCTCTGGTTGTCTATCTGCCgtgt                                >  2:298273/1‑140 (MQ=255)                                                                                                       tttCTTTATTGATGACGAAAATTCCCGCACGCTGTTCCTTGGGTATTCCGTCGCTGGCCAGCGTCTTAAAGCGCAATTTGCCCAGCAAGGCCGTATCGTCGATGCTGATAACCCTCTGTTTGTCTATCTGCCGTgtggtg                            >  1:526943/1‑140 (MQ=255)                                                                                                             tATTGATGACGAAAATTCCCGCACGCTGTTCCTTGGGTATTCCGTCGCTGGCCAGCGTCTTAAAGCGCAATTTGCCCAGCAAGGCCGTATCGTCGATGCTGATAACCCTCTGTTTGTCTATCTGGCGTGTGGTGTTGGCg                      <  2:658847/140‑1 (MQ=255)                                                                                                             tATTGATGACGAAAATTCCCGCACGCTGTTCCTTGGGTATTCCGTCGCTGGCCAGCGTCTTAAAGCGCAATTTGCCCAGCAAGGCCGTATCGTCGATGCTGATAACCCTCTGTTTGTCTATCTGCCGTGTGGTGTTGGCg                      <  2:406895/140‑1 (MQ=255)                                                                                                             tATTGATGACGAAAATTCCCGCACGCTGTTCCTTGGGTATTCCGTCGCTGGCCAGCGTCTTAAAGCGCAATTTGCCCAGCAAGGCCGTATCATCGATGCTGATAGCCCTCTGTTTGTGTATCGGCCGTGTGGTGTTGGCg                      >  2:170729/1‑140 (MQ=255)                                                                                                                                cGCACGCTGTTCCTTGGGTATTCCGTCGCTGGCCAGCGTCTTAAAGCGCAATTTGCCCAGCAAGGCCGTATCGTCGATGCTGATAACCCTCTGTTTGTCTATCTGCCGTGTGGTGTTGGCGGTGGTCCTGGTGGCGTCGc   >  2:508423/1‑140 (MQ=255)                                                                                                                                 gCACGCTGTTCCTTGGGTATTCCGTCGCTGGCCAGCGTCTTAAAGCGCAATTTGCCCAGCAAGGCCGTATCGTCGATGCTGATAACCCTCTGTTTGTCTATCTGCCGTGTGGTGTTGGCGGTGGTCCTGGTGGCGTCGCa  >  1:551547/1‑140 (MQ=255)                                                                                                                                      |                                                                                                                                       AGCGAAACTGCGCAGCCATGGCGTTACGGTCGTGGAATATGAGCAAGATTATGGTGTTGCCGTCGAGGAAGGACGTAAAGCAGCGCAGTCTGACCCGAACTGTTTCTTTATTGATGACGAAAATTCCCGCACGTTGTTCCTTGGGTATTCCGTCGCTGGCCAGCGTCTTAAAGCGCAATTTGCCCAGCAAGGCCGTATCGTCGATGCTGATAACCCTCTGTTTGTCTATCTGCCGTGTGGTGTTGGCGGTGGTCCTGGTGGCGTCGCA  >  NC_000913/2479798‑2480065

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑