Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,490,509	T→C	D481G (GAC→GGC)	oxc ←	oxalyl‑CoA decarboxylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,490,509	0	T	C	100.0%	122.3 / NA	38	D481G (GAC→GGC)	oxc	oxalyl‑CoA decarboxylase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (25/13);  total (25/13)

TAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCTTGCATGGTGCAACAGATCCGTTGGTGATGGTGCACCAGCGCCACTGAGATCAACACCGTCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGAGAACCAGAAGT  >  NC_000913/2490373‑2490645                                                                                                                                         |                                                                                                                                         tAAAGCATGACGAAGGTCATCTATCCTGGCGACGTTACAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCTTGCATGGTGCAACAGATCCGTTGGTGATGGTGCACCAGCGCCACTGAGATCAACACCGcctc                                                                                                                                       <  1:177784/140‑1 (MQ=255)                ttCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCTTGCATGGTGCAACAGATCCGTTGGTGATGGTGCACCAGCGCCACTGAGATCAACACCGCCTCCTCTGTAGATgccgc                                                                                                                        <  1:414689/140‑1 (MQ=255)                                          caccGCCACGAAACGCATCCATTAATTTGTCATACCTTGCATGGTGCAACAG‑TCCGTTGGTGATGGTGCACCACCGCCACTGAGATCAACACCGCCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCg                                                                                             <  2:547951/137‑1 (MQ=255)                                           aaCGCCACGAAACGCATCCATTAATTTGTCATACCTTGCATGGTGCAACAGATCCGTTGGTGATGGTGCACCAGCGCCACTGAGATCAACACCGCCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCg                                                                                             <  2:587965/140‑1 (MQ=255)                                            aCGCCACGAAACGCATCCATTAATTTGTCATACCTTGCATGGTGCAACAGATCCGTTGGTGATGGTGCACCAGCGCCACTGAGATCAACACCGCCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCgg                                                                                            >  2:455496/1‑140 (MQ=255)                                             cGCCACGAAACGCATCCATTAATTTGTCATACCTTGCATGGTGCAACAGATCCGTTGGTGATGGTGCACCAGCGCCACTGAGATCAACACCGCCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCg                                                                                             <  2:40988/138‑1 (MQ=255)                                             cGCCACGAAACGCATCCATTAATTTGTCATACCTTGCATGGTGCAACAGATCCGTTGGTGATGGTGCACCAGCGCCACTGAGATCAACACCGCCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCg                                                                                             >  1:40988/1‑138 (MQ=255)                                             cGCCACGAAACGCATCCATTAATTTGTCATACCTTGCATGGTGCAACAGATCCGTTGGTGATGGTGCACCAGCGCCACTGAGATCAACACCGCCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGc                                                                                           >  2:636484/1‑140 (MQ=255)                                             cGCCACGAAACGCATCCATTAATTTGTCATACCTTGCATGGTGCAACAGATCCGTTGGTGATGGTGCACCAGCGCCACTGAGATCAACACCACCTCCTCTGTAGATGCAGCCATTATAAAAACTAACGATCGTCACCGGc                                                                                           >  2:286784/1‑140 (MQ=255)                                             cGCCACGAAACGCATCCATTAATTTGTCATACCTTGCATGGTGCAACAGATCCGTTGGTGATGGTGCACCAGCGCCACTGAGATCAACACCACCTCCTCTCTAGTTGCGGCCATTATTAAAATTAACGATCGTCACCGGc                                                                                           >  2:296600/1‑140 (MQ=255)                                                     aaCGCATCCATTAATTTGTCATACCTTGCATGGTGCAACAGATCCGTTGGTGATGGTGCACCAGCGCCACTGAGATCAACACCGCCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTtata                                                                                   <  1:379863/140‑1 (MQ=255)                                                               ttAATTTGTCATACCTTGCATGGTGCAACAGATCCGTTGGTGATGGTGCACCAGCGCCACTGAGATCAACACCGCCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCATCGGCAGGTTATATCGACAAATc                                                                         >  1:258283/1‑140 (MQ=255)                                                                       tCATACCTTGCATGGTGCAACAGATCCGTTGGTGATGGTGCACCAGCGCCACTGAGATCAACACCGCCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAAt                                                                 >  2:362011/1‑140 (MQ=255)                                                                               tGCATGGTGCAACAGATCCGTTGGTGATGGTGCACCAGCGCCACTGAGATCAACACCGCCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACGGGCAGGTGATACCGACAAATCGTTTCAATTTCCATcc                                                         >  1:301453/1‑140 (MQ=255)                                                                               tGCATGGTGCAACAGATCCGTTGGTGATGGTGCACCAGCGCCACTGAGATCAACACCGCCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATcc                                                         >  2:416757/1‑140 (MQ=255)                                                                               tGCATGGTGCAACAGATCCGTTGGTGATGGTGCACCAGCGCCACTGAGATCAACACCGCCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACAGGCAGGTGATAACGACAAATCGTTTCAATTTCCATcc                                                         >  1:292506/1‑140 (MQ=255)                                                                                 cATGGTGCAACAGATCCGTTGGTGATGGTGCACCAGCGCCACTGAGATCAACACCGCCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCa                                                       >  1:549078/1‑140 (MQ=255)                                                                                 cATGGTGCAACAGATCCGTTGGTGATGGTGCACCAGCGCCACTGAGATCAACACCGCCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCa                                                       >  1:372845/1‑140 (MQ=255)                                                                                 cATGGTGCAACAGATCCGTTGGTGATGGTGCACCAGCGCCACTGAGATCAACACCGCCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCa                                                       >  2:358509/1‑140 (MQ=255)                                                                                    ggTGCAACATATCCGTTGGTGATGGTGCACCAGCGCCACTGAGATCAACACCGCCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTg                                                    <  2:64381/140‑1 (MQ=255)                                                                                    ggTGCAACAGATCCGTTGGTGATGGTGCACCAGCGCCACTGAGATCAACACCGCCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTg                                                    <  1:636484/140‑1 (MQ=255)                                                                                    ggTGCAACAGATCCGTTGGTGATGGTGCACCAGCGCCACTGAGATCAACACCGCCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTg                                                    >  2:96945/1‑140 (MQ=255)                                                                                      tGCAACAGATCCGTTGGTGATGGTGCACCAGCGCCACTGAGATCAACACCGCCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCCGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGaa                                                  >  2:601904/1‑140 (MQ=255)                                                                                      tGCAACAGATCCGTTGGTGATGGTGCACCAGCGCCACTGAGATCAACACCGCCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGaa                                                  >  2:560551/1‑140 (MQ=255)                                                                                      tGCAACAGATCCGTTGGTGATGGTGCACCAGCGCCACTGAGATCAACACCGCCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGaa                                                  >  1:392959/1‑140 (MQ=255)                                                                                             gATCCGTTGGTGATGGTACACCTGCGCCACTAAGACCAAAACCGCCTCTTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCaaaa                                           <  2:258283/140‑1 (MQ=255)                                                                                                ccGTTGGTGATGGTGCACCAGCGCCACTGAGATCAACACCGCCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACGAATCGTTGCAAGTTGCATCCCACTGAAACCAAAAGCa                                        >  1:315930/1‑140 (MQ=255)                                                                                                ccGTTGGTGATGGTGCACCAGCGCCACTGAGATCAACACCGCCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCa                                        >  2:110752/1‑140 (MQ=255)                                                                                                ccGTTGGTGATGGTGCACCAGCGCCACTGAGATCAACACCGCCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGATTATATCGACAAGTCGTTTCAATTTTCATCCCACTGAAACCAAAAGCa                                        >  2:255466/1‑140 (MQ=255)                                                                                                    tGGTGATGGTGCACCAGCGCCACTGAGATCAACACCGCCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAg                                          >  2:2764/1‑134 (MQ=255)                                                                                                    tGGTGATGGTGCACCAGCGCCACTGAGATCAACACCGCCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAg                                          <  1:2764/134‑1 (MQ=255)                                                                                                    tGGTGATGGTGCACCAGCGCCACTGAGATCAACACCGCCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTAt                                    <  1:110752/140‑1 (MQ=255)                                                                                                          tGGTGCACCAGCGCCACTGAGATCAACACCGCCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCtt                              >  1:155896/1‑140 (MQ=255)                                                                                                              gCACCAGCGCCACTGAGATCAACACCGCCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCTTCAAt                          >  1:583641/1‑140 (MQ=255)                                                                                                                 ccAGCGCCACTGAGATCAACACCGCCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCTTCAATGGc                       <  2:549078/140‑1 (MQ=255)                                                                                                                      gcCACTGAGATCAACACCGCCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCTTCAATGGCGACaa                  >  2:135957/1‑140 (MQ=255)                                                                                                                               aTCAACACCGCCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCTCACTGAAAGCAAGAGCCCTATCACCCTCAATGGCGACAACCGGAGAAc         >  2:211221/1‑140 (MQ=255)                                                                                                                                  aaCACCGCCTCCTCTGTAGATGACGTCATCATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGAGAACCAg      <  1:222207/140‑1 (MQ=255)                                                                                                                                      ccGCCTCCTCTGTAGATGCCGCTATTATTGAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGAGAACCAGAAGt  <  1:255466/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                         TAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCTTGCATGGTGCAACAGATCCGTTGGTGATGGTGCACCAGCGCCACTGAGATCAACACCGTCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGAGAACCAGAAGT  >  NC_000913/2490373‑2490645

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑