Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,509,057	G→A	P124S (CCG→TCG)	glk ←	glucokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,509,057	0	G	A	100.0%	146.6 / NA	44	P124S (CCG→TCG)	glk	glucokinase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (24/20);  total (24/20)

TCTTCACTATTCGGCGCAAAATCAACGTGACCGCCTTCGCCTGGCAAGCTTACCCAACGCTTATCGACATGGACCAGATGCGCAACCCCAAGCCCCGTTCCGGCACCGTAAACCGCAATAGGCTTACCTTCGACCGGTTCTGCGCCACCAAACTGAATCAGATGCTCTTTTTTCAGCATCGGGATCGCCATCGATACAGCGGTAAAATCGTTAATAATTTCCAGATGGCTAAAACCGAGATTCTTTTTCATTTCGGCAATTGAGAACGC  >  NC_000913/2508921‑2509189                                                                                                                                         |                                                                                                                                     tcttcACTATTCGGCGCAAAATCAACGTGACCGCCTTCGCCTGGCAAGCTTACCCAACGCTTATCGACATGGACCAGATGCGCAACCCCAAACCCCGTTCGGGCGCCGCAAACCGCACTAGGCTTACCTTCGACCGATTc                                                                                                                                   >  2:230224/1‑140 (MQ=255)          ttCGGCGCAAAATCAACGTGACCGCCTTCGCCTGGCAAGCTTACCCAACGCTTATCGACATGGACCAGATGCGCAACCCCAAGCCCCGTTCTGGCACCGTCAACTGCAGTAGGCTTATCTTCGACCGATTCTGCGccacc                                                                                                                          >  2:185524/1‑140 (MQ=255)          ttCGGCGCAAAATCAACGTGACCGCCTTCGCCTGGCAAGCTTACCCAACGCTTATCGACATGGACCAGATGCGCAACCCCAAGCCCCGTTCCGGCACCGTAAACCGCAATAGGCTTACCTTCGACCGATTCTGCaccacc                                                                                                                          >  1:450514/1‑140 (MQ=255)          ttCGGCGCAAAATCAACGTGACCGCCTTCGCCTGGCAAGCTTACCCAACGCTTATCGACATGGACCAGATGCGCAACCCCAAGCCCCGTTCCGGCACCGTAAACCGCAATAGGCTTACCTTCGACCGATTCTGCGccacc                                                                                                                          >  1:534536/1‑140 (MQ=255)          ttCGGCGCAAAATCAACGTGACCGCCTTCGCCTGGCAAGCTTACCCAACGCTTATCGACATGGACCAGATGCGCAACCCCAAGCCCCGTTCCGGCACCGTAAACCGCAATAGGCTTACCTTCGACCGATTCTGCGccacc                                                                                                                          >  1:453496/1‑140 (MQ=255)            cGGCGCAAAATCAACGTGACCGCCTTCGCCTGGCAAGCTTACCCAACGCTTATCGACATGGACCAGATGCGCAACCCCAAGCCCCGTTCCGGCACCGTAAACCGCAATAGGCTTACCTTCGACCGATTCTGCGCCACCaa                                                                                                                        >  1:661181/1‑140 (MQ=255)            cGGCGCAAAATCAACATGACCGCCTCCGCCTGGCAAGCTTACCCAACGCTTATCGACATGGACCAGATGCGCAACCCCAAGCCCCGTTCCGGCACCGTAAACCGCAATAGGCTTACCTTCGACCGATTCTGCGCCACCaa                                                                                                                        <  1:185524/140‑1 (MQ=255)             ggCGCAAAATCAACGTGACCGCCTTCGCCTGGCAAGCTTACCCAACGCTTATCGACATGGACCAGATGCGCAACCCCAAGCCCCGTTCCGGCACCGTAAACCGCAATAGGCTTACCTTCGACCGATTCTGCGCCACCaaa                                                                                                                       <  1:532692/140‑1 (MQ=255)               cgcAAAATCAACGTGACCGCCTTCGCCTGGCAAGCTTACCCAACGCTTATCGACATGGACCAGATGCGCAACCCCAAGCCCCGTTCCGGCACCGTAAACCGCAATAGGCTTACCTTCGACCGATTCTGCGCCACCAAACt                                                                                                                     >  2:539390/1‑140 (MQ=255)                        aaCGTGACCGCCTTCCCCTGGCTAGTTTACCCAACGCCTATCGACATGGACCAGATGCGCAACCCCAAGCCCCGTTCCGGCACCGTAAACCGCAATAGGCTTACCTTCGACCGATTCTGCGCCACCAAACTGAATCAGAt                                                                                                            <  1:169265/140‑1 (MQ=255)                          cGTGACCGCCTTCGCCTGGCAAGCTTACCCAACGCTTATCGACATGGACCAGATGCGCAACCCCAAGCCCCGTTCCGGCACCGTAAACCGCAATAGGCTTACCTTCGACCGATTCTGCGCCACCAAACTGAATCAGATGc                                                                                                          <  2:19174/140‑1 (MQ=255)                                    ttCGCCTGGCAAGCTTACCCAACGCTTATCGACATGGACCAGATGCGCAACCCCAAGCCCCGTTCCGGCACCGTAAACCGCAATAGGCTTACCTTCGACCGATTCTGCGCCACCAAACTGAATCAGATGCTCTTTTTTCa                                                                                                <  2:453496/140‑1 (MQ=255)                                         cTGGCAAGCTTACCCAACGCTTATCGACATGGACCAGATGCGCAACCCCAAGCCCCGTTCCGGCACCGTAAACCGCAATAGGCTTACCTTCGACCGATTCTGCGCCACCAAACTGAATCAGATGCTCTTTTTTCAGCATc                                                                                           <  1:539390/140‑1 (MQ=255)                                               aGCTTACCCAACGCTTATCGACATGGACCAGATGCGCAACCCCAAGCCCCGTTCCGGCACCGTAAACCGCAATAGGCTTACCTTCGACCGATTCTGCGCCACCAAACTGAATCAGATGCTCTTTTTTCAGCATCGGGATc                                                                                     <  1:615650/140‑1 (MQ=255)                                               aGCTTACCCAACGCTAATCGACCTGGACCGGATGCGCGACCCCAAGCCCCGTTCCGGCACCGTAAACCGCAATAGGCTTACCTTCGACCGATTCTGCGCCACCAAACTGAATCAGATGCTCTTTTTTCAGCATCGGGATc                                                                                     <  1:224650/140‑1 (MQ=255)                                                      ccAACGCTTATCGACATGGACCAGATGCGCAACCCCAAGCCCCGCTCCGGCACCGTAAACCGCAATAGGCTTACCTTCGACCGATTCTGCGCCACCAAACGGAATCAGCTGCTCTTTGTTCAACATCGGGATCGCCATCg                                                                              >  2:224650/1‑140 (MQ=255)                                                          cGCTTATCGACATGGACCAGATGCGCAACCCCAAGCCCCGTTCCGGCACCGTAAACCGCAATAGGCTTACCTTCGACCGATTCTGCGCCACCAAACTGAATCAGATGCTCTTTTTTCAGCATCGGGATCGCCATCGATAc                                                                          <  2:661181/140‑1 (MQ=255)                                                           gCTTATCGACATGGACCAGATGCGCAACCCCAAGCCCCGTTCCGGCACCGTAAACCGCAATAGGCTTACCTTCGACCGATTCTGCGCCACCAAACTGAATCAGATGCTCTTTTTTCAGCATCGGGATCGCCATCGATACa                                                                         >  1:445009/1‑140 (MQ=255)                                                           gCTTATCGACATGGACCAGATGCGCAACCCCAAGCCCCGTTCCGGCACCGTAAACCGCAATAGGCTTACCTTCGACCGATTCTGCGCCACCAAACTGAATCAGAGGCTTTTTTTTCAGCATCTGGATCGCCATCGATACa                                                                         >  2:277602/1‑140 (MQ=255)                                                            cTTATCGACATGGACCAGATGCGCAACCCCAAGCCCCGTTCCGGCACCGTAAACCGCAATAGGCTTACCTTCGACCGATTCTGCGCCACCAAACTGAATCAGCTGCTCTTGTTTTAGTATCGGGATCGCCATCGATACAg                                                                        >  1:232467/1‑140 (MQ=255)                                                               aTCGACATGGACCAGATGCGCAACCCCAAGCCCCGTTCCGGCACCGTAAACCGCAATAGGCTTACCTTCGACCGATTCTGCGCCACCAAACTGAATCAGATGCTCTTTTTTCAGCATCGGGATCCCCACCGATACAGCgg                                                                     >  1:367674/1‑140 (MQ=255)                                                               aTCGACATGGACCAGATGCGCAACCCCAAGCCCCGTTCCGGCACCGTAAACCGCAATAGGCTTACCTTCGACCGATTCTGCGCCACCAAACGGAATCAGACGCTATTTGTTCAGCATGGGGAGCGCCATCGATACAGCgg                                                                     >  2:194362/1‑140 (MQ=255)                                                                  gACATGGACCAGATGCGCAACCCCAAGCCCCGTTCCGGCACCGTAAACCGCAATAGGCTTACCTTCGACCGATTCTGCGCCACCAAACTGAATCAGATGCTCTTTTTTCAGCATCGGGATCGCCATCGATACAGCGGTaa                                                                  >  1:461535/1‑140 (MQ=255)                                                                   aCATGGACCAGATGCGCAACCCCAATCCCCGTTCCGGCACCGTAAACCGCAATAGGCTTACCTTCGACCGATTCTGCGCCACCAAACTGAATCAGATGCTCTTTTTTCAGCATCGGGATCGCCATCGATACAGCGGTaaa                                                                 <  1:277602/140‑1 (MQ=255)                                                                     aTGGACCAGATGCGCAACCCCAAGCCCCGTTCCGGCACCGTAAACCGCAATAGGCTTACCTTCGACCGATTCTGCGCCACCAAACTGAATCAGATGCTCTTTTTTCAGCATCGGGATCGCCATCGATACAGCGGTAAAAt                                                               >  2:449842/1‑140 (MQ=255)                                                                     aTGGACCAGATGCGCAACCCCAAGCCCCGTTCCGGCACCGTAAACCGCAATAGGCTTACCTTCGACCGATTCTGCGCCACCAAACTGAATCAGATGCTCTTTTTTCAGCATCGGGATCGCCATCGATACAGCGGTAAAAt                                                               >  2:657721/1‑140 (MQ=255)                                                                       ggACCAGATGCGCAACCCCAAGCCCCGTTCCGGCACCGTAAACCGCAATAGGCTTACCTTCGACCGATTCTGCGCCACCAAACTGAATCAGATGCTCTTTTTTCAGCATCGGGATCGCCATCGATACAGCGGTAAAATCg                                                             >  2:644987/1‑140 (MQ=255)                                                                        gACCAGATGCGCAACCCCAAGCCCCGTTCCGGCACCGTAAACCGCAATAGGCTTACCTTCGACCGATTCTGCGCCACCAAACTGAATCAGATGCTCTTTTTTCAGCATCGGGATCGCCATCGATACAGCGGTAAAATCGt                                                            <  1:644987/140‑1 (MQ=255)                                                                            aGATGCGCAACCCCAAGCCCCGTTCCGGCACCGTAAACCGCAATAGGCTTACCTTCGACCGATTCTGCGCCACCAAACTGAATCAGATGCTCTTTTTTCAGCATCGGGATCGCCATCGATACAGCGGTAAAATCGTtaat                                                        <  2:49840/140‑1 (MQ=255)                                                                              aTGCGCAACCCCAAGCCCCGTTCCGGCACCGTAAACCGCAATAGGCTTACCTTCGACCGATTCTGCGCCACCAAACTGAATCAGATGCTCTTTTTTCAGCATCGGGATCGCCATCGATACAGCGGTAAAATCGTtaataa                                                      >  1:369793/1‑140 (MQ=255)                                                                                   caaccccaacccCCGTTCCGGCACCGTAAACCGCAATAGGCTTACCTTCGACCGATTCTGCGCCACCAAACTGAATCAGATGCTCTTTTTTCAGCATCGGGATCGCCATCGATACAGCGGTAAAATCGTTAATAATTTcc                                                 >  2:66752/1‑140 (MQ=255)                                                                                   cAACCCCAAGCCCCGTTCCGGCACCGTAAACCGCAATAGGCTTACCTTCGACCGATTCTGCGCCACCAAACTGAATCAGATGCTCTTTTTTCAGCATCGGGATCGCCATCGATACAGCGGTAAAATc                                                              <  2:402042/127‑1 (MQ=255)                                                                                   cAACCCCAAGCCCCGTTCCGGCACCGTAAACCGCAATAGGCTTACCTTCGACCGATTCTGCGCCACCAAACTGAATCAGATGCTCTTTTTTCAGCATCGGGATCGCCATCGATACAGCGGTAAAATc                                                              >  1:402042/1‑127 (MQ=255)                                                                                   cAACCCCAAGCCCCGTTCCGGCACCGTAAACCGCAATAGGCTTACCTTCGACCGATTCTGCGCCACCAAACTGAATCAGATGCTCTTTTTTCAGCATCGGGATCGCCATCGATACAGCGGTAAAATCGTTAATAATTTcc                                                 >  1:545046/1‑140 (MQ=255)                                                                                           aGCCCCGTTCCGGCACCGTAAACCGCAATAGGCTTACCTTCGACCGATTCTGCGCCACCAAACTGAATCAGATGCTCTTTTTTCAGCATCGGGATCGCCATCGATACAGCGGTAAAATCGTTAATAATTTCCAGATGGCt                                         >  1:444827/1‑140 (MQ=255)                                                                                                     cGGCACCGTAAACCGCAATAGGCTTACCTTCGACCGATTCTGCGCCACCAAACTGAATCAGATGCTCTTTTTTCAGCATCGGGATCGCCATCGATACAGCGGTAAAATCGTTAATAATTTCCAGATGGCTAAAACCgaga                               <  2:534536/140‑1 (MQ=255)                                                                                                      ggCACCGTAAACCGCAATAGGCTTACCTTCGACCGATTCTGCGCCACCAAACTGAATCAGATGCTCTTTTTTCAGCATCGGGATCGCCATCGATACAGCGGTAAAATCGTTAATAATTTCCAGATGGCTAAAACCGAGAt                              <  2:369793/140‑1 (MQ=255)                                                                                                             tAAACCGCAATAGGCTTACCTTCGACCGATTCTGCGCCACCAAACTGAATCAGATGCTCTTTTTTCAGCATCGGGATCGCCATCGATACAGCGGTAAAATCGTTAATAATTTCCAGATGGCTAAAACCGAGATTCttttt                       >  1:65245/1‑140 (MQ=255)                                                                                                                        aGGCTTACCTTCGACCGATTCTGCGCCACCAAACTGAATCAGATGCTCTTTTTTCAGCATCGGGATCGCCATCGATACAGCGGTAAAATCGTTAATAATTTCCAGATGGCTAAAACCGAGATTCTTTTTCATTTCGGCaa            <  2:65245/140‑1 (MQ=255)                                                                                                                              aCCTTCGACCGATTCTGCGCCACCAAACTGAATCAGATGCTCTTTTTTCAGCATCGGGATCGCCATCGATACAGCGGTAAAATCGTTAATAATTTCCAGATGGCTAAAACCGAGATTCTTTTTCATTTCGGCAATTgaga      >  1:470919/1‑140 (MQ=255)                                                                                                                               ccTTCGACCGATTCTGCGCCACCAAACTGAATCAGATGCTCTTTTTTCAGCATCGGGATCGCCATCGATACAGCGGTAAAATCGTTAATAATTTCCAGATGGCTAAAACCGAGATTCTTTTTCATTTCGGCAATTGAGaa     >  1:470235/1‑140 (MQ=255)                                                                                                                                cTTCGACCGATTCTGCGCCACCAAACTGAATCAGATGCTCTTTTTTCAGCATCGGGATCGCCATCGATACAGCGGTAAAATCGTTAATAATTTCCAGATGGCTAAAACCGAGATTCTTTTTCATTTCGGCAATTGAGAAc    <  2:446567/140‑1 (MQ=255)                                                                                                                                 ttCGACCGATTCTGCGCCACCAAACTGAATCAGATGCTCTTTTTTCAGCATCGGGATCGCCATCGATACAGCGGTAAAATCGTTAATAATTTCCAGATGGCTAAAACCGAGATTCTTTTTCATTTCGGCAATTGAGAACg   <  2:450514/140‑1 (MQ=255)                                                                                                                                 ttCGACCGATTCTGCGCCACCAAACTGAATCAGATGCTCTTTTTTCAGCATCGGGATCGCCATCGATACAGCGGTAAAATCGTTAATAATTTCCAGATGGCTAAAACCGAGATTCTTTTTCATTTCGGCAATTGAGAACg   <  1:449842/140‑1 (MQ=255)                                                                                                                                 ttCGACCGATTCTGCCCCACCACACTGAAGCAGATGCCCTTTTTTCAGCATCGGGATCGCCATCGATACAGCGGTAAAATCGTTAATAATTTCCAGATGGCTAAAACCGAGATTCTTTTTCATTTCGGCAATTGAGAACg   <  1:230224/140‑1 (MQ=255)                                                                                                                                  tCGACCGATTCTGCGCCACCAAACTGAATCAGATGCTCTTTTTTCAGCATCGGGATCGCCATCGATACAGCGGTAAAATCGTTAATAATTTCCAGATGGCTAAAACCGAGATTCTTTTTCATTTCGGCAATTGAGAACGc  <  1:657721/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                     TCTTCACTATTCGGCGCAAAATCAACGTGACCGCCTTCGCCTGGCAAGCTTACCCAACGCTTATCGACATGGACCAGATGCGCAACCCCAAGCCCCGTTCCGGCACCGTAAACCGCAATAGGCTTACCTTCGACCGGTTCTGCGCCACCAAACTGAATCAGATGCTCTTTTTTCAGCATCGGGATCGCCATCGATACAGCGGTAAAATCGTTAATAATTTCCAGATGGCTAAAACCGAGATTCTTTTTCATTTCGGCAATTGAGAACGC  >  NC_000913/2508921‑2509189

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑