Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,511,730	A→C	V326G (GTG→GGG)	mntH ←	Mn(2(+))/Fe(2(+)): H(+) symporter MntH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,511,730	0	A	C	100.0%	32.6 / NA	11	V326G (GTG→GGG)	mntH	Mn(2(+))/Fe(2(+)): H(+) symporter MntH
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (9/2);  total (9/2)

CGCCAGAGCGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATCTAATCCCATCAGAATGACAATAAATGACGGCAACATGGTGACTGTACGACGCACCCACAGCGGGATATGAAAGCGAATGAAGCCCTGCATCACCACCTGCCCCGCCAGTGTCCCCACCACCGTTGAAGACAGCCCCGCAGCAACCAGGCTTAATCCAAAGACCGTTGCCGCAGCGTGGCTTAACAGCG  >  NC_000913/2511618‑2511865                                                                                                                 |                                                                                                                                        cgcCAGAGCGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATCTAATCCCATCAGAATGACAATAAATGACGGCAACATGGTGACTGTACGACGCCCCCACAGCGGGATATGAAAGCGAATGa                                                                                                              >  1:549697/1‑140 (MQ=255)         cGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATCTAATCCCATCAGAATGACAATAAATGACGGCAACATGGTGACTGTACGACGCCCCCACAGCGGGATATGAAAGCGAATGAAGCCCTGc                                                                                                      <  2:549697/140‑1 (MQ=255)                  aaCTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATCTAATCCCATCAGAATGACAATAAATGACGGCAACATGGTGACTGTACGACGCCCCCACAGCGGGATATGAAAGCGAATGAAGCCCTGCATCaccacc                                                                                             <  2:624019/140‑1 (MQ=255)                       aaCAGTACCTGACTCATAACCAGAATCCGTGTCGGATCTAATCCCATCAGAATGACAATAAATGACGGCAACATGGTGACTGTACGACGCCCCCACAGCGGGGTATGAAATCGAGTGCAGCCCTACATCACCACCTGccc                                                                                        >  1:316702/1‑140 (MQ=255)                           gTACCTGACTCATAACAAGAACCCGTGTCGGCTCACACCCCAACAGAAAGAAAAAAAAGGACGGCAACATGGTGACTGTCCGCCCCCCCCCCAGCGGGATATAAAAGCGAATGAAGCCCTGCACCCCCCCCTGCCCCGcc                                                                                    <  2:194721/140‑1 (MQ=255)                            tACCTGACTCATAACCAGAATCCGTGTCGGATCTAATCCCATCAGAATGACAATAAATGACGGCAACATGGTGACTGTACGACGCCCCCACAGCGGGATATGAAAGCGAATGAAGCCCTGCATCACCACCTGCCCCGCCa                                                                                   >  1:149508/1‑140 (MQ=255)                            tACCTGACTCATAACCAGAATCCGTGTCGGATCTAATCCCATCAGAATGACAATAAATGACGGCAACATGGTGACTGTACGACGCCCCCACAGCGGGATATGAAAGCGAATGAAGCCCTGCATCACCACCTGCCCCGCCa                                                                                   >  1:56893/1‑140 (MQ=255)                                      ctAACCAGCATCCGTGTCGGATCTAATCCAATCAGAAGGAGAATAAATGACTGCACTATGGCGACTGTACGATGCCCCCACAGCGGGCTATGATAGCGAATGAAACGCTGCAACACCACCTGCCCCGCCACTGTCCccac                                                                         >  2:533633/2‑140 (MQ=255)                                         aCCAGAATCCGTGTCGGATCTAATCCCATCAGAATGACAATAAATGACGGCAACATGGTGACTGTACGACGCCCCCACAGCGGGATATGAAAGCGAATGAAGCCCTGCATCACCACCTGCCCCGCCAGTGTCCCcaccac                                                                      >  1:501812/1‑140 (MQ=255)                                                tCCGTGTCGGATCTAATCCCATCAGAATGACAATAAATGACGGCAACATGGTGACTGTACGACGCCCCCACAGCGGGATATGAAAGCGAATGAAGCCCTGCATCACCACCTGCCCCGCCAGTGTCCCCACCACCGTTGaa                                                               >  1:34677/1‑140 (MQ=255)                                                                      aaaaaTGAAAAAAAATGACGGAAACAGGGGCCCTTGACGACCCCCCCACGGGGGGATATGAAAGCGAATGAAGCCCGGCACCCCCCCCTCCCCCGCCAGTGTCCCCACCACCGTTGAAGACAGCCCCGCAGCAACCAGGc                                         <  2:149508/137‑1 (MQ=255)                                                                                         ggCAACATGGTGACTGTACGACGCCCCCACAGCGGGATATGAAAGCGAATGAAGCCCTGCATCACCACCTGCCCCGCCAGTGTCCCCACCACCGTTGAAGACAGCCCCGCAGCAACCAGGCTTAATCCAAAGACCGTTGc                      >  2:546733/1‑140 (MQ=255)                                                                                                           cgacgCCCCCACAGCGGGATATGAAAGCGAATGAAGCCCTGCATCACCACCTGCCCCGCCAGTGTCCCCACCACCGTTGAAGACAGCCCCGCAGCAACCAGGCTTAATCCAAAGTCCCTTGCCGTAGCGTGGCTTAACAg    >  1:279474/1‑140 (MQ=255)                                                                                                             acgCCCCCACAGCGGGATATGAAAGCGAATGAAGCCCTGCATCACCACCTGCCCCGCCAGTGTCCCCACCACCGTTGAAGACAGCCCCGCAGCAACCAGGCTTAATCCAAAGACCGTTGCCGCAGCGTGGCTTAACAGCg  >  2:505941/1‑140 (MQ=255)                                                                                                                 |                                                                                                                                        CGCCAGAGCGATACCAAAACTTAACAGTACCTGACTCATAACCAGAATCCGTGTCGGATCTAATCCCATCAGAATGACAATAAATGACGGCAACATGGTGACTGTACGACGCACCCACAGCGGGATATGAAAGCGAATGAAGCCCTGCATCACCACCTGCCCCGCCAGTGTCCCCACCACCGTTGAAGACAGCCCCGCAGCAACCAGGCTTAATCCAAAGACCGTTGCCGCAGCGTGGCTTAACAGCG  >  NC_000913/2511618‑2511865

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑