Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,527,795	T→C	F284L (TTC→CTC)	yfeH →	putative solute:Na(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,527,795	0	T	C	100.0%	135.5 / NA	39	F284L (TTC→CTC)	yfeH	putative solute:Na(+) symporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (20/19);  total (20/19)

TTCTTCTGGCTATCGTGATTGTAGTTAACGTCTTTATGGCACGCCGACTGAGCTTCAATAAGGCAGATGAAATTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGTGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGACCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGC  >  NC_000913/2527658‑2527931                                                                                                                                          |                                                                                                                                         ttcttcTGGCTATCGTGATTGTAGTTAACGTCTTTATGGCACGCCGACTGAGCTTCAATAAGGCAGATGAAATTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAGATGGCAGCCCGATGGCAAACATTCTGCTc                                                                                                                                        >  1:193789/1‑140 (MQ=255)  tcttctGGCTATCGTGATTGTAGTTAACGTCTTTATGGCACGCCGACTGAGCTTCAATAAGGCAGATGAAATTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGCTcc                                                                                                                                       <  1:570503/140‑1 (MQ=255)              cGTGATTGTAGTTAACGTCTTTATGGCACGCCGACTGAGCTTCAATAAGGCAGATGAAATTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGCTCCCCACATCGGTGa                                                                                                                           >  1:45750/1‑140 (MQ=255)                  aTTGTAGTTAACGTCTTTATGGCACGCCGACTGAGCTTCAATAAGGCAGATGAAATTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGCTCCCCACATCGGTGATCgg                                                                                                                       <  2:642603/140‑1 (MQ=255)                       aGTTAACGTCTTTATGGCACGCCGACTGAGCTTCAATAAGGCAGATGAAATTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGCTCCCCACATCGGTGATCGGTatga                                                                                                                  >  1:104229/1‑140 (MQ=255)                        gTTAACGTCTTTATGGCACGCCGACTGAGCTTCAATAAGGCAGATGAAATTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGCTCCCCACATCGGTGATCGGTatgat                                                                                                                 <  2:516864/140‑1 (MQ=255)                                 tttATGGCACGCCGACTGAGCTTCAATAAGGCAGATGAAATTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGCTCCCCACATCGGTGATCGGTATGATGGCGCTGcc                                                                                                        >  1:329840/1‑140 (MQ=255)                                 tttATGGCACGCCGACTGAGCTTCAATAAGGCAGATGAAATTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGCTCCCCACATCGGTGATCGGTATGATGGCGCTGcc                                                                                                        <  1:35125/140‑1 (MQ=255)                                 tttATGGCACGCCGACTGAGCTTCAATAAGGCAGATGAAATTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGCTCCCCACATCGGTGATCGGTATGATGGCGCTGcc                                                                                                        >  1:591167/1‑140 (MQ=255)                                       gCACGCCGACTGAGCTTCAATAAGGCAGATGAAATTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGCTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCtgat                                                                                                  <  1:490258/140‑1 (MQ=255)                                        cACGCCGACTGAGCTTCAATAAGGCAGATGAAATTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGCTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCtgatg                                                                                                 >  2:523415/1‑140 (MQ=255)                                        cACGCCGACTGAGCTTCAATAAGGCAGATGAAATTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGCTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCtgatg                                                                                                 >  1:583799/1‑140 (MQ=255)                                          cGCCGACTGAGCTTCAATAAGGCAGATGAAATTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGCTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTgatgat                                                                                               >  2:528462/1‑140 (MQ=255)                                          cGCCGACTGAGCTTCAATAAGGCAGATGAAATTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGCTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTgatgat                                                                                               >  2:663189/1‑140 (MQ=255)                                               aCTGAGCTTCAATAAGGCAGATGAAATTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGCTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTcc                                                                                          <  1:663189/140‑1 (MQ=255)                                               aCTGAGCTTCAATAAGGCAGATGAAATTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGCTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTcc                                                                                          <  1:528462/140‑1 (MQ=255)                                                        cAATAAGGCAGATGAAATTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGCTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATcc                                                                                 <  2:421017/140‑1 (MQ=255)                                                              ggCAGATGAAATTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGCTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGa                                                                           >  2:78519/1‑140 (MQ=255)                                                                   aTGAAATTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGCTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTc                                                                      >  1:164197/1‑140 (MQ=255)                                                                   aTGAAATTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGCTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTc                                                                      >  2:39041/1‑140 (MQ=255)                                                                   aTGAAATTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGCTCCCCACATCGGTGATCGGCATGATGGCACTGCCCCCGATGATTTTCCATCAGATCCAATTGATGGTc                                                                      >  2:221322/1‑140 (MQ=255)                                                                       aaTTACTATCGTCTTGTGTGGTTCGAAAAGGAGTCTGTCAAATGGCATCCCGATGGCAAACATTCTGCTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCtgtg                                                                  <  1:251365/140‑1 (MQ=255)                                                                                      ttGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGCTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTc                                                   >  1:423024/1‑140 (MQ=255)                                                                                       tgtgGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGCTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCg                                                  >  2:74274/1‑140 (MQ=255)                                                                                       tgtgGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGCTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGAAGATTTTCCGTCAAATCGAATTGATGATCTGGGCGGTGCTGGCGCGTCg                                                  >  2:187327/1‑140 (MQ=255)                                                                                         tgGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGCTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGAt                                                >  1:26517/1‑140 (MQ=255)                                                                                                  aaaGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGCTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGcc                                       <  2:104229/140‑1 (MQ=255)                                                                                                      agTCTGGCAAATGGCATCCCGATGGCAAACATTCTGCTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGAc                                   >  2:498077/1‑140 (MQ=255)                                                                                                         cTGGCAAATGGCATCCCGATGGCAAACATTCTGCTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGACCGa                                <  1:403189/140‑1 (MQ=255)                                                                                                          tGGCAAATGGCATCCCGATGGCAAACATTCTGCTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGACCGaa                               <  2:591167/140‑1 (MQ=255)                                                                                                                aTGGCATCCCGATGGCAAACATTCTGCTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGACCGAACAGTTa                         >  1:454064/1‑140 (MQ=255)                                                                                                                 tGGCATCCCGATGGCAAACATTCTGCTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGACCGAACAGTTAc                        <  2:423024/140‑1 (MQ=255)                                                                                                                      tCCCGATGGCAAACATTCTGCTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGACCGAACAGTTACAGgcg                   >  1:660992/1‑140 (MQ=255)                                                                                                                      tCCCGATGGCAAACATTCTGCTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGACCGAACAGTTACAGgcg                   <  1:498077/140‑1 (MQ=255)                                                                                                                       cccGATGGCAAACATTCTGCTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGACCGAACAGTTACAGgcgc                  <  2:454064/140‑1 (MQ=255)                                                                                                                            tGGCAAACATTCTGCTCCCCACCTCTGTGCTCGGTATCATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGACCGAACAGTTACAGGCGcagcag             <  1:221322/140‑1 (MQ=255)                                                                                                                            tGGCAAACATTCTGCTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGACCGAACAGTTACAGGCGcagcag             <  1:39041/140‑1 (MQ=255)                                                                                                                                  aCATTCTGCTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGACCGAACAGTTACAGGCGCAGCAGGAAagc       <  1:428412/140‑1 (MQ=255)                                                                                                                                       cTGCTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGACCGAACAGTTACAGGCGCAGCAGGAAAGCAgcgc  <  1:523415/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                         TTCTTCTGGCTATCGTGATTGTAGTTAACGTCTTTATGGCACGCCGACTGAGCTTCAATAAGGCAGATGAAATTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGTGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGACCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGC  >  NC_000913/2527658‑2527931

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑