Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,527,823	T→C	V293A (GTG→GCG)	yfeH →	putative solute:Na(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,527,823	0	T	C	100.0%	143.5 / NA	40	V293A (GTG→GCG)	yfeH	putative solute:Na(+) symporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (19/21);  total (19/21)

TTTATGGCACGCCGACTGAGCTTCAATAAGGCAGATGAAATTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGTGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGACCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGATAAAGCTTAAAGCGGACGCTTCA  >  NC_000913/2527690‑2527957                                                                                                                                      |                                                                                                                                       tttATGGCACGCCGACTGAGCTTCAATAAGGCAGATGAAATTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGCTCCCCACATCGGTGATCGGTATGATGGCGCTGcc                                                                                                                                  >  1:329840/1‑140 (MQ=255) tttATGGCACGCCGACTGAGCTTCAATAAGGCAGATGAAATTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGCTCCCCACATCGGTGATCGGTATGATGGCGCTGcc                                                                                                                                  <  1:35125/140‑1 (MQ=255) tttATGGCACGCCGACTGAGCTTCAATAAGGCAGATGAAATTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGCTCCCCACATCGGTGATCGGTATGATGGCGCTGcc                                                                                                                                  >  1:591167/1‑140 (MQ=255)       gCACGCCGACTGAGCTTCAATAAGGCAGATGAAATTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGCTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCtgat                                                                                                                            <  1:490258/140‑1 (MQ=255)        cACGCCGACTGAGCTTCAATAAGGCAGATGAAATTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGCTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCtgatg                                                                                                                           >  2:523415/1‑140 (MQ=255)        cACGCCGACTGAGCTTCAATAAGGCAGATGAAATTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGCTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCtgatg                                                                                                                           >  1:583799/1‑140 (MQ=255)          cGCCGACTGAGCTTCAATAAGGCAGATGAAATTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGCTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTgatgat                                                                                                                         >  2:528462/1‑140 (MQ=255)          cGCCGACTGAGCTTCAATAAGGCAGATGAAATTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGCTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTgatgat                                                                                                                         >  2:663189/1‑140 (MQ=255)               aCTGAGCTTCAATAAGGCAGATGAAATTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGCTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTcc                                                                                                                    <  1:663189/140‑1 (MQ=255)               aCTGAGCTTCAATAAGGCAGATGAAATTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGCTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTcc                                                                                                                    <  1:528462/140‑1 (MQ=255)                        cAATAAGGCAGATGAAATTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGCTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATcc                                                                                                           <  2:421017/140‑1 (MQ=255)                              ggCAGATGAAATTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGCTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGa                                                                                                     >  2:78519/1‑140 (MQ=255)                                   aTGAAATTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGCTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTc                                                                                                >  2:39041/1‑140 (MQ=255)                                   aTGAAATTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGCTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTc                                                                                                >  1:164197/1‑140 (MQ=255)                                   aTGAAATTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGCTCCCCACATCGGTGATCGGCATGATGGCACTGCCCCCGATGATTTTCCATCAGATCCAATTGATGGTc                                                                                                >  2:221322/1‑140 (MQ=255)                                       aaTTACTATCGTCTTGTGTGGTTCGAAAAGGAGTCTGTCAAATGGCATCCCGATGGCAAACATTCTGCTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCtgtg                                                                                            <  1:251365/140‑1 (MQ=255)                                                      ttGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGCTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTc                                                                             >  1:423024/1‑140 (MQ=255)                                                       tgtgGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGCTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCg                                                                            >  2:74274/1‑140 (MQ=255)                                                       tgtgGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGCTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGAAGATTTTCCGTCAAATCGAATTGATGATCTGGGCGGTGCTGGCGCGTCg                                                                            >  2:187327/1‑140 (MQ=255)                                                         tgGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGCTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGAt                                                                          >  1:26517/1‑140 (MQ=255)                                                                  aaaGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGCTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGcc                                                                 <  2:104229/140‑1 (MQ=255)                                                                      agTCTGGCAAATGGCATCCCGATGGCAAACATTCTGCTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGAc                                                             >  2:498077/1‑140 (MQ=255)                                                                         cTGGCAAATGGCATCCCGATGGCAAACATTCTGCTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGACCGa                                                          <  1:403189/140‑1 (MQ=255)                                                                          tGGCAAATGGCATCCCGATGGCAAACATTCTGCTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGACCGaa                                                         <  2:591167/140‑1 (MQ=255)                                                                                aTGGCATCCCGATGGCAAACATTCTGCTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGACCGAACAGTTa                                                   >  1:454064/1‑140 (MQ=255)                                                                                 tGGCATCCCGATGGCAAACATTCTGCTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGACCGAACAGTTAc                                                  <  2:423024/140‑1 (MQ=255)                                                                                      tCCCGATGGCAAACATTCTGCTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGACCGAACAGTTACAGgcg                                             >  1:660992/1‑140 (MQ=255)                                                                                      tCCCGATGGCAAACATTCTGCTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGACCGAACAGTTACAGgcg                                             <  1:498077/140‑1 (MQ=255)                                                                                       cccGATGGCAAACATTCTGCTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGACCGAACAGTTACAGgcgc                                            <  2:454064/140‑1 (MQ=255)                                                                                            tGGCAAACATTCTGCTCCCCACCTCTGTGCTCGGTATCATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGACCGAACAGTTACAGGCGcagcag                                       <  1:221322/140‑1 (MQ=255)                                                                                            tGGCAAACATTCTGCTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGACCGAACAGTTACAGGCGcagcag                                       <  1:39041/140‑1 (MQ=255)                                                                                                  aCATTCTGCTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGACCGAACAGTTACAGGCGCAGCAGGAAagc                                 <  1:428412/140‑1 (MQ=255)                                                                                                       cTGCTCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGACCGAACAGTTACAGGCGCAGCAGGAAAGCAgcgc                            <  1:523415/140‑1 (MQ=255)                                                                                                           tCCCCACATCGGTGATCTGTATGATGGCGCTCCCCTTGACGATTTTCCGTCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGACCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGAt                        <  2:196157/140‑1 (MQ=255)                                                                                                           tCCCCACATCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGACCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGAt                        <  2:503419/140‑1 (MQ=255)                                                                                                               cacaTCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGACCAAACAGTTACAGGCGCATCAGGGAAGCAGCGCCGATAAAg                    >  2:199534/1‑140 (MQ=255)                                                                                                                  aTCGGTGATCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGACCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGATAAAGCtt                 >  2:361600/1‑140 (MQ=255)                                                                                                                          tCGGTATGATGGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGACCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGATAAAGCTTAAAGCGGa         <  1:36266/140‑1 (MQ=255)                                                                                                                                tgatgGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGACCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGATAAAGCTTAAAGCGGACGCTTc   <  1:78519/140‑1 (MQ=255)                                                                                                                                 gatgGCGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGACCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGATAAAGCTTAAAGCGGACGCTTCa  <  2:329840/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                       TTTATGGCACGCCGACTGAGCTTCAATAAGGCAGATGAAATTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGTGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGACCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGATAAAGCTTAAAGCGGACGCTTCA  >  NC_000913/2527690‑2527957

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑