Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,601,506	C→T	S102S (AGC→AGT)	hyfA →	hydrogenase 4 component A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,601,506	0	C	T	100.0%	150.9 / NA	45	S102S (AGC→AGT)	hyfA	hydrogenase 4 component A
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (20/25);  total (20/25)

GCCCCTTGCCTGCAGGTCTGCCCGGTCAATGCCATCTCTCAGAGGGATGATGCGATCCAACTCAACGAAAGCCTCTGTATTGGCTGCAAGCTTTGCGCCGTGGTCTGCCCATTTGGCGCAATCAGCGCTTCAGGAAGCCGTCCGGTGAATGCCCATGCGCAATATGTTTTTCAGGCTGAAGGCTCACTCAAAGACGGCGAAGAAAACGCGCCAACACAACATGCTTTGCTGCGCTGGGAACCTGGTGTCCAGACCGTCGCGGTGAAATGCGACC  >  NC_000913/2601369‑2601642                                                                                                                                          |                                                                                                                                         gCCCCTTGCCTGCAGGTCTGCCCGGTCAATGCCATCTCTCAGAGGGATGATGCGATCCAACTCAACGAAAGCCTCTGTATTGGCTGCAAGCTTTGCGCCGTGGTCTGCCCATTTGGCGCAATCAGCGCTTCAGGAAGTCg                                                                                                                                        >  2:48508/1‑140 (MQ=255)  ccccTTGCCTGCAGGTCTGCCCGGTCAATGCCATCTCTCAGAGGGATGATGCGATCCAACTCAACGAAAGCCTCTGTATTGGCTGCAAGCTTTGCGCCGTGGGCTGCCCAGTTGACGAAATCAGAGCTTCAGGAAGTCGt                                                                                                                                       >  1:215533/1‑140 (MQ=255)  ccccTTGCCTGCAGGTCTGCCCGGTCAATGCCATCTCTCAGAGGGATGATGCGATCCAACTCAACGAAAGCCTCTGTATTGGCTGCAAGCTGTGCGCCGTAGTCTGCCGATTTGGCGGAATCTGCGCTTCAGGAAGTCGt                                                                                                                                       >  2:177718/1‑140 (MQ=255)     cTTGCCCGCAGGTCTGCCCGGTCAATGCCATCTCTCAGAGGGATGATGCGATCCAACTCAACGAAAGCCTCTGTATTGGCTGCAAGCTTTGCGCCGTGGTCTGCCCATTTGGCGCAATCAGCGCTTCAGGAAGTCGTCCg                                                                                                                                    <  2:8739/140‑1 (MQ=255)      ttGCCTGCAGGTCTGCCCGGTCAATGCCATCTCTCAGAGGGATGATGCGATCCAACTCAACGAAAGCCTCTGTATTGGCTGCAAGCTTTGCTCCGTGGTCTGCCCATTCGGCGCAATCAGCGGTTCAGGAAGTCGTCCgg                                                                                                                                   >  2:280379/1‑140 (MQ=255)      ttGCCTGCAGGTCTGCCCGGTCAATGCCATCTCTCAGAGGGATGATGCGATCCAACTCAACGAAAGCCTCTGTATTGGCTGCAAGCTTTGCGCCGTGGTCTGCCCATTTGGCGCAATCAGCGCTTCAGGAAGTCGTCCgg                                                                                                                                   >  2:435140/1‑140 (MQ=255)          cTGCAGGTCTGCCCGGTCAATGCCATCTCTCAGAGGGATGATGCGATCCAACTCAACGAAAGCCTCTGTATTGGCTGCAAGCTTTGCGCCGTGGTCTGCCCATTTGGCGCAATCAGCGCTTCAGGAAGTCGTCCGGTGaa                                                                                                                               <  1:505076/140‑1 (MQ=255)          cTGCAGGTCTGCCCGATCAATGTCAGCTCTCAGAGGGTTGATGCGATCCAACTCAACGAAAGCCTCTGTATTGGCTGCAAGCTTTGCGCCGTGGTCTGCCCATTTGGCGCAATCAGCGCTTCAGGAAGTCGTCCGGTGaa                                                                                                                               <  1:285590/140‑1 (MQ=255)                gTCTGCCCGGTCAATGCCATCTCTCAGAGGGATGATGCGATCCAACTCAACGAAAGCCTCTGTATTGGCTGCAAGCTTTGCGCCGTGGTCTGCCCATTTGGCGCAATCAGCGCTTCAGGAAGTCGTCCGGTGAATGCCCa                                                                                                                         >  1:22976/1‑140 (MQ=255)                    gCCCGGTCAATGCCATCTCTCAGAGGGATGATGCGATCCAACTCAACGAAAGCCTCTGTATTGGCTGCAAGCTTTGCGCCGTGGTCTGCCCATTTGGCGCAATCAGCGCTTCAGGAAGTCGTCCGGTGAATGCCCATgcg                                                                                                                     <  1:607755/140‑1 (MQ=255)                      ccGGTCAATGCCATCTCTCAGAGGGATGATGCGATCCAACTCAACGAAAGCCTCTGTATTGGCTGCAAGCTTTGCGCCGTGGTCTGCCCATTTGGCGCAATCAGCGCTTCAGGAAGTCGTCCGGTGAATGCCCATGCGCa                                                                                                                   <  2:75537/140‑1 (MQ=255)                      ccGGTCAATGCCATCTCTCAGAGGGATGATGCGATCCAACTCAACGAAAGCCTCTGTATTGGCTGCAAGCTTTGCGCCGTGGTCTGCCCATTTGGCGCAATCAGCGCTTCAGGAAGTCGTCCGGTGAATGCCCATGCGCa                                                                                                                   <  1:469743/140‑1 (MQ=255)                      ccGGTCAATGCCATCGCTCAGATGGATGAGGCGATCCGACTCAACGAAAGCCTCTGTATTGGCTGCAAGCTTTGCGCCGTGGTCTGCCCATTTGGCGCAATCAGCGCTTCAGGAAGTCGTCCGGTGAATGCCCATGCGCa                                                                                                                   <  1:308267/140‑1 (MQ=255)                          tCAATGCCATCTCTCAGAGGGATGATGCGATCCAACTCAACGAAAGCCTCTGTATTGGCTGCAAGCTTTGCGCCGTGGTCTGCCCATTTGGGGCAATCAGGGCTTCTGGAAGTCGTCGGGTGGATGCCCATGCGCAatat                                                                                                               >  2:290155/1‑140 (MQ=255)                          tCAATGCCATCTCTCAGAGGGATGATGCGATCCAACTCAACGAAAGCCTCTGTATTGGCTGCAAGCTTTGCGCCGTGGTCTGCCCATTTGGCGCAATCAGCGCTTCAGGAAGTCGTCCGGTGAATGCCCATGCGCAatat                                                                                                               >  2:429736/1‑140 (MQ=255)                          tCAATGCCATCTCTCAGAGGGATGATGCGATCCAACTCAACGAAAGCCTCTGTATTGGCTGCAAGCTTTGCGCCGTGGTCTGCCCATTTGGCGCAATCAGCGCTTCAGGAAGTCGTCCGGTGAATGCCCATGCGCAatat                                                                                                               >  2:72624/1‑140 (MQ=255)                          tCAATGCCATCTCTCAGAGGGATGATGCGATCCAACTCAACGAAAGCCTCTGTATTGGCTGCAAGCTTTGCGCCGTGGTCTGCCCATTTGGCGCAATCAGCGCTTCAGGAAGTCGTCCGGTGAATGCCCATGCGCAatat                                                                                                               >  2:104142/1‑140 (MQ=255)                                 cATCTCTCAGAGGGATGTTGCGCTCCAACTCGACGTAAGCCTCTGTATTGGCTGCAAGCTTTGCGCCGTGGTCTGCCCATTTGGCGCAATCAGCGCTTCAGGAAGTCGTCCGGTGAATGCCCATGCGCAATATGTTTTTc                                                                                                        <  2:215533/140‑1 (MQ=255)                                           agGGATGATGCGATCCAACTCAACGAAAGCCTCTGTATTGGCTGCAAGCTTTGCGCCGTGGTCTGCCCATTTGGCGCAATCAGCGCTTCAGGAAGTCGTCCGGTGAATGCCCATGCGCAATATGTTTTTCAGGCTGAAgg                                                                                              <  1:429736/140‑1 (MQ=255)                                             ggATGATGCGATCCATCTCAACCAACGCCGCTGTATTTGCTGCAAGCTTTGCGCCGTGGTCTGCCCATTTGGCGCAATCAGCGCTTCAGGAAGTCGTCCGGTGAATGCCCATGCGCAATATGTTTTTCAGGCTGAAGGct                                                                                            <  1:177718/140‑1 (MQ=255)                                             ggATGATGCGATCCAGCTCAACGAATGCCTCTGTATTGGCTGCAAGCTTTGCGCCGTGGTCTGCCCATTTGGCGCAATCAGCGCTTCAGGAAGTCGTCCGGTGAATGCCCATGCGCAATATGTTTTTCAGGCTGAAGGct                                                                                            <  1:280379/140‑1 (MQ=255)                                             ggATGATGCGATCCAACTCAACGAAAGCCTCTGTATTGGCTGCAAGCTTTGCGCCGTGGTCTGCCCATTTGGCGCAATCAGCGCTTCAGGAAGTCGTCCGGTGATTTCGCATGCGCAATATGTTTTTCAGGATGAAGGct                                                                                            >  2:310854/1‑140 (MQ=255)                                             ggATGATGCGATCCAACTCAACGAAAGCCTCTGTATTGGCTGCAAGCTTTGCGCCGTGGTCTGCCCATTTGGCGCAATCAGCGCTTCAGGAAGTCGTCCGGTGAATGCCCATGCGCAATATGTTTTTCAGGCTGAAGGct                                                                                            <  1:139178/140‑1 (MQ=255)                                                       aTCCAACTCAACGAAAGCCTCTGTATTGGCTGCAAGCTTTGCGCCGTGGTCTGCCCATTTGGCGCAATCAGCGCTTCAGGAAGTCGTCCGGTGAATGCCCATGCGCAATATGTTTTTCAGGCTGAAGGCTCACTCAAAGa                                                                                  >  1:129288/1‑140 (MQ=255)                                                                     aaGCCTCTGTATTGGGTGCAAGGTTTGCGACGTGGTCGGCCCATTTGGCGCAATCAGCGCTTCAGGAAGTCGTCCGGTGAATGCCCATGCGCAATATGTTTTTCAGGCTGAAGGCTCACTCAAAGACGGCGAAGAAAAcg                                                                    <  1:260592/140‑1 (MQ=255)                                                                       gCCTCTGTATTGGCTGCAAGCTTTGCGCCGTGGTCTGCCCATTTGGCGCAATCAGCGCTTCAGGAAGTCGTCCGGTGAATGCCCATGCGCAATATGTTTTGCAGGCTGTAGGCTCACACAAAGACGGCGAAGAAAAcgcg                                                                  >  2:260592/1‑140 (MQ=255)                                                                          tctGTATTGGCTGCAGGCTTTGCGCGGTGGTCTGCCCATTTGGCGCAATCAGCGCTTCAGGAAGTCGTCCGGTGAATGCCCATGCGCAATATGTTTTTCAGGCTGAAGGCTCACTCAAAGACGGCGAAGAAAACGCGCca                                                               <  1:222007/140‑1 (MQ=255)                                                                          tctGTATTGGCTGCAAGCTTTGCGCCGTGGTCTGCCCATTTGGCGCAATCAGCGCTTCAGGAAGTCGTCCGGTGAATGCCCATGCGCAATATGTTTTTCAGGCTGAAGGCTCACTCAAAGACGGCGAAGAAAACGCGCca                                                               <  2:458793/140‑1 (MQ=255)                                                                            tGTATTGGCTGCAAGCTTTGCGCCGTGGTCTGCCCATTTGGCGCAATCAGCGCTTCAGGAAGTCGTCCGGTGAATGCCCATGCGCAATATGTTTTTCAGGCTGAAGGCTCACTCAAAGACGGCGAAGAAAACGCGCcaac                                                             >  1:527073/1‑140 (MQ=255)                                                                                 tGGCTGCAAGCTTTGCGCCGTGGTCTGCCCATTTGGCGCAATCAGCGCTTCAGGAAGTCGTCCGGTGAATGCCCATGCGCAATATGTTTTTCAGGCTGAAGGCTCACTCAAAGACGGCGAAGAAAACGCGCcaacacaac                                                        <  1:435140/140‑1 (MQ=255)                                                                                   gCTGCAAGCTTTGCGCCGTGGTCTTCCCATTTGGCGCAATCAGCGCTTCAGGAAGTCGTCCGGTGAATGCCCATGGCCAATATGTTTTTCAGGCTGAAGGCTCACTCAAAGACGGCGAAGAAAACGCGCCAACACAACAt                                                      >  2:562143/1‑140 (MQ=255)                                                                                   gCTGCAAGCTTTGCGCCGTGGTCTGCCCATTTGGCGCAATCAGCGCTTCAGGAAGTCGTCCGGTGAATGCCCATGCGCAATATGTTTTTCAGGCTGAAGGCTCACTCAAAGACGGCGAAGAAAACGCGCCAACACAACAt                                                      >  2:430587/1‑140 (MQ=255)                                                                                       cAAGCTTTGCGCCGTGGTCTGCCCATTTGGCGCAATCAGCGCTTCAGGAAGTCGTCCGGTGAATGCCCATGCGCAATATGTTTTTCAGGCTGAAGGCTCAGTCAGAGAGGGCGAAGAGAACGAGCCAACACAACATGCtt                                                  >  2:175891/1‑140 (MQ=255)                                                                                        aaGCTTTGCGCCGTGGTCTGCCCATTTGGCGCAATCAGCGCTTCAGGAAGTCGTCCGGTGAATGCCCATGCGCAATATGTTTTTCAGGCTGAAGGCTCACTCAAAGACGGCGAAGAAAACGCGCcaacaca                                                          >  2:14040/1‑131 (MQ=255)                                                                                        aaGCTTTGCGCCGTGGTCTGCCCATTTGGCGCAATCAGCGCTTCAGGAAGTCGTCCGGTGAATGCCCATGCGCAATATGTTTTTCAGGCTGAAGGCTCACTCAAAGACGGCGAAGAAAACGCGCcaacaca                                                          <  1:14040/131‑1 (MQ=255)                                                                                        aaGCTTTGCGCCGTGGTCTGCCCATTTGGCGCAATCAGCGCTTCAGGAAGTCGTCCGGTGAATGCCCATGCGCAATATGTTTTTCAGGCTGAAGGCTCACTCAAAGACGGCGAAGAAAACGCGCCAACACAACATGCttt                                                 >  2:411732/1‑140 (MQ=255)                                                                                            tttGCGCCGTGGTCTGCCCATTTGGCGCAATCAGCGCTTCAGGAAGTCGTCCGGTGAATGCCCATGCGCAATATGTTTTTCAGGCTGAAGGCTCACTCAAAGACGGCGAAGAAAACGCGCCAACACAACATGCTTtgctg                                             <  2:22976/140‑1 (MQ=255)                                                                                                                       cAATCAGCGCTTCAGGAAGTCGTCCGGTGAATGCCCATGCGCAATATGTTTTTCAGGCTGAAGGCTCACTCAAAGACGGCGAAGAAAACGCGCCAACACAACATGCTTTGCTGCGCTGGGAACCTGGTGTCCAGACCGTc                  <  1:562143/140‑1 (MQ=255)                                                                                                                        aaTCAGCGCTTCAGGAAGTCGTCCGGTGAATGCCCATGCGCAATATGTTTTTCAGGCTGAAGGCTCACTCAAAGACGGCGAAGAAAACGCGCCAACACAACATGCTTTGCTGCGCTGGGAACCTGGTGTCCAGACCGTcg                 >  2:503838/1‑140 (MQ=255)                                                                                                                               gcTTCAGGAAGTCGTCCGGTGAATGCCCATGCGCAATATGTTTTTCAGGCTGAAGGCTCACTCAAAGACGGCGAAGAAAACGCGCCAACACAACATGCTTTGCTGCGCTGGGAACCTGGTGTCCAGACCGTCGCGGTGaa          >  2:22992/1‑140 (MQ=255)                                                                                                                                  tCAGGAAGTCGTCCGGTGAATGCCCATGCGCAATATGTTTTTCAGGCTGAAGGCTCACTCAAAGACGGCGAAGAAAACGCGCCAACACAACATGCTTTGCTGCGCTGGGAACCTGGTGTCCAGACCGTCGCGGTGAAATg       <  1:72624/140‑1 (MQ=255)                                                                                                                                  tCAGGAAGTCGTCCGGTGAATGCCCATGCGCAATATGTTTTTCAGGCTGAAGGCTCACTCAAAGACGGCGAAGAAAACGCGCCAACACAACATGCTTTGCTGCGCTGGGAACCTGGTGTCCAGACCGTCGCGGTGAAATg       <  1:104142/140‑1 (MQ=255)                                                                                                                                  tCAGGAAGTCGTCCGCTGAATGGCCATGCTCAATATGGTTTTCAGGCTGAAGGCTCACTCAAAGACGGCGAAGAAAACGCGCCAACACAACATGCTTTGCTGCGCTGGGAACCTGGTGTCCAGACCGTCGCGGTGAAATg       <  1:290155/140‑1 (MQ=255)                                                                                                                                      gAAGTCGTCCGGTGAATGCCCATGCGCAATATGTTTTTCAGGCTGAAGGCTCACTCAAAGACGGCGAAGAAAACGCGCCAACACAACATGCTTTGCTGCGCTGGGAACCTGGTGTCCAGACCGTCGCGGTGAAATGCGAc   <  1:100349/140‑1 (MQ=255)                                                                                                                                      gAAGTCGTCCGGTGAATGCCCATGCGCAATATGTTTTTCAGGCTGAAGGCTCACTCAAAGACGGCGAAGAAAACGCGCCAACACAACATGCTTTGCTGCGCTGGGAACCTGGTGTCCAGACCGTCGCGGTGAAATGCGAc   <  2:527073/140‑1 (MQ=255)                                                                                                                                      gAAGTCGTCCGGTGAATGCCCATGCGCAATATGTTTTTCAGGCTGAAGGCTCACTCAAAGACGGCGAAGAAAACGCGCCAACACAACATGCTTTGCTGCGCTGGGAACCTGGTGTCCAGACCGTCGCGGTGAAATGCGAc   <  1:411732/140‑1 (MQ=255)                                                                                                                                       aaGTCGTCCGGTGAATGCCCATGCGCAATATGTTTTTCAGGCTGAAGGCTCACTCAAAGACGGCGAAGAAAACGCGCCAACACAACATGCTTTGCTGCGCTGGGAACCTGGTGTCCAGACCGTCGCGGTGAAATGCGAcc  <  1:22992/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                         GCCCCTTGCCTGCAGGTCTGCCCGGTCAATGCCATCTCTCAGAGGGATGATGCGATCCAACTCAACGAAAGCCTCTGTATTGGCTGCAAGCTTTGCGCCGTGGTCTGCCCATTTGGCGCAATCAGCGCTTCAGGAAGCCGTCCGGTGAATGCCCATGCGCAATATGTTTTTCAGGCTGAAGGCTCACTCAAAGACGGCGAAGAAAACGCGCCAACACAACATGCTTTGCTGCGCTGGGAACCTGGTGTCCAGACCGTCGCGGTGAAATGCGACC  >  NC_000913/2601369‑2601642

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑