Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,602,306	T→C	A163A (GCT→GCC)	hyfB →	hydrogenase 4 component B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,602,306	0	T	C	100.0%	109.6 / NA	32	A163A (GCT→GCC)	hyfB	hydrogenase 4 component B
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (12/20);  total (12/20)

AATATTTTCATCGCATCGATGGTTGCCCTGCTGGTGATGGACAACGCTTTTTGGTTCATCGTGCTGTTTGAAATGATGTCGCTGTCTTCCTGGTTTCTGGTCATTGCCAGGCAGGATAAAACGTCGATCAACGCTGGCATGCTCTACTTTTTTATCGCCCACGCCGGATCGGTGCTGATAATGATCGCCTTCTTGCTGATGGGGCGCGAAAGCGGCAGCCTCGATTTTGCCAGTTTCCGCACGCTTTCACTTTCTCCGGGGCTG  >  NC_000913/2602172‑2602435                                                                                                                                       |                                                                                                                                  aaTATTTTCATCGCATCGATGGTTGCCCTGCTGGTGATGGACAACGCTTTTTGGTTCATCGTGCTGTTTGAAATGATGTCGCTGTCTTCCTGGTTTCTGGTCATTGCCAGGCAGGATAAAACGTCGATCAACGCCGGCAt                                                                                                                              <  2:492302/140‑1 (MQ=255)             gcatcgATGGTTGCCCTGCTGGTGATGGACAACGCTTTTTGGTTCATCGTGCTGTTTGAAATGATGTCGCTGTCTTCCTGGTTTCTGGTCATTGCCAGGCAGGATAAAACGTCGATCAACGCCGACATGCTCTACttttt                                                                                                                  >  1:299869/1‑140 (MQ=255)                         gCCCTGCTGGTGATGGACAACGCTTTTTGGTTCATCGTGCTGTTTGAAATGATGTCGCTGTCTTCCTGGTTTCTGGTCATTGCCAGGCAGGATAAAACGTCGATCAACGCCGGCATGCTCTACTTTTTTATCGcc                                                                                                           >  2:490858/1‑135 (MQ=255)                         gCCCTGCTGGTGATGGACAACGCTTTTTGGTTCATCGTGCTGTTTGAAATGATGTCGCTGTCTTCCTGGTTTCTGGTCATTGCCAGGCAGGATAAAACGTCGATCAACGCCGGCATGCTCTACTTTTTTATCGcc                                                                                                           <  1:490858/135‑1 (MQ=255)                              gctgGTGATGGACAACGCTTTTTGGTTCATCGTGCTGTTTGAAATGATGTCGCTGTCTTCCTGGTTTCTGGTCATTGCCAGGCAGGATAAAACGTCGATCAACGGCGGAGTGCTCTATTTTTAAATCGCCCGCGCCGGAt                                                                                                 >  2:323668/1‑140 (MQ=255)                              gctgGTGATGGACAACGCTTTTTGGTTCATCGTGCTGTTTGAAATGATGTCGCTGTCTTCCTGGTTTCTGGTCATTGCCAGGCAGGATAAAACGTCGATCAACGCCGGCATGCTCTACTTTTTTATCGCCCACGCCGGAt                                                                                                 <  2:650029/140‑1 (MQ=255)                              gctgGTGATGGACAACGCTTTTTGGTTCATCGTGCTGTTTGAAATGATGTCGCTGTCTTCCTGGTTTCTGGTCATTGCCAGGCAGGATAAAACGTCGATCAACGCCGGCATGCTCTACTTTTTTATCGCCCACGCCGGAt                                                                                                 >  2:547503/1‑140 (MQ=255)                                 ggTGATGGACAACGCTTTTTGGTTCATCGTGCTGTTTGAAATGATGTCGCTGTCTTCCTGGTTTCTGGTCATTGCCAGGCAGGATAAAACGTCGATCAACGCCGGCATGCTCTACTTTTTTATCGCCCACGCCGGATCgg                                                                                              >  2:30228/1‑140 (MQ=255)                                  gTGATGGACAACGCTTTTTGGTTCATCGTGCTGTTTGAAATGGTGTCGCTGTCTTCCTGGTTTCTGGTCATTGCCAGGCAGGATAAAACGTCGATCAACGCCGGCATGCTCTACTTTTTTATCGCCCACGCCGGATCGGt                                                                                             <  2:486549/140‑1 (MQ=255)                                  gTGATGGACAACGCTTTTTGGTTCATCGTGCTGTTTGAAATGATGTCGCTGTCTTCCTGGTTTCTGGTCATTGCCAGGCAGGATAAAACGTCGATCAACGCCGGCATGCTCTACTTTTTTATCGCCCACGCCGGATCGGt                                                                                             >  1:392339/1‑140 (MQ=255)                                   tGATGGACAACGCTTTTTGGTTCATCGTGCTGTTTGAAATGATGTCGCTGTCTTCCTGGTTTCTGGTCATTGCCAGGCAGGATAAAACGTCGATCAACGCCGGCATGCTCTACTTTTTTATCGCCCACGCCGGATCGGTg                                                                                            <  2:632580/140‑1 (MQ=255)                                   tGATGGACAACGCTTTTTGGTTAATTGTGCTGTTTGACATGATGTCGCTGTCTTCCTGGTTTCTGGTCATTGCCAGGCAGGATAAAACGTCGATCAACGCCGGCATGCTCTACTTTTTTATCGCCCACGCCGGATCGGTg                                                                                            <  1:268731/140‑1 (MQ=255)                                    gATGGACAGCGC‑TTTTGGTTCATCGTGCTGTTTGAAATGATGTCGCTGTCTTCCTGGTTTCTGGTCATTGCCAGGCAGGATAAAACGTCGATCAACGCCGGCATGCTCTACTTTTTTATCGCCCACGCCGGATCGGTGCt                                                                                          >  2:502800/1‑140 (MQ=255)                                    gATGGACAACGCTTTTTGGTTCATCGTGCTGTTTGAAATGATGTCGCTGTCTTCCTGGTTTCTGGTCATTGCCAGGCAGGATAAAACGTCGATCAACGCCGGCATGCTCTACTTTTTTATCGCCCACGCCGGATCGGTGc                                                                                           >  2:101907/1‑140 (MQ=255)                                       ggACAACGCTTTTTGGTCCATCCTGCTGTTTTAAATGATGTCGCTGTCTTCCTGGTTTCTGGTCATTGCCAGGCAGGATAAAACGTCGATCAACGCCGGCATGCTCTACTTTTTTATCGCCCACGCCGGATCGGTGCTGa                                                                                        <  2:214697/140‑1 (MQ=255)                                         aCAACGCTTTTTGGTTCATCGTGCTGTTTGAAATGATGTCGCTGTCTTCCTGGTTTCTGGTCATTGCCAGGCAGGATAAAACGTCGATCAACGCCGGCATGCTCTACTTTTTTATCGCCCACGCCGGATCGGTGCTGATa                                                                                      >  1:488901/1‑140 (MQ=255)                                              gCTTTTTGGTTCATCGTGCTGTTTGAAATGATGTCGCTGTCTTCCTGGTTTCTGGTCATTGCCAGGCAGGATAAAACGTCGATCAACGCCGGCATGCTCTACTTTTTTATCGCCCACGCCGGATCGGTGCTGATAATGAt                                                                                 <  1:101907/140‑1 (MQ=255)                                              gCTTTTTGGTTCATCGTGCTGTTTGAAATGATGTCGCTGTCTTCCTGGTTTCTGGTCATTGCCAGGCAGGATAAAACGTCGATCAACGCCGGCATGCTCTACTTTTTTATCGCCCACGCCGGATCGGTGCTGATAATGAt                                                                                 <  1:30228/140‑1 (MQ=255)                                              gCTTTTTGGTTCATCGTGCTGTTTGAAATGATGTCGCTGTCTTCCTGGTTTCTGGTCATTGCCAGGCAGGATAAAACGTCGATCAACGCCGGCATGCTCTACTTTTTTATCGCCCACGCCGGATCGGTGCTGATAATGAt                                                                                 <  2:58143/140‑1 (MQ=255)                                              gCTTTTTGGTTCATCGTGCTGTTTGAAATGATGTCGCTGTCTTCCTGGTTTCTGGTCATTGCCAGGCAGGATAAAACGTCGATCAACGCCGGCATGCTCTACTTTTTTATCGCCCACGCCGGATCGGTGCTGATAATGAt                                                                                 <  1:547503/140‑1 (MQ=255)                                              cgcttttGGTTCATCGTGCTGTTTGAAATGATGTCGCTGTCTTCCTGGTTTCTGGTCATTGCCAGGCAGGATAAAACGTCGATCAACGCCGGCATGCTCTACTTTTTTATCGCCCACGCCGGATCGGTGCTGATAATGAt                                                                                 <  1:502800/137‑1 (MQ=255)                                                tttttGGTTCATCGTGCTGTTTCAAATGATGTCGCTGTCCTCCTGGTTTCTGGTCATTGCCAGGCAGGATAAAACGTCGATCAACGCCGGCATGCTCTACTTTTTTATCGCCCACGCCGGATCGGTGCTGATAATGATCg                                                                               <  2:263219/140‑1 (MQ=255)                                                    tGGTTCATCGTGCTGTTTGAAATGATGTCGCTGTCTTCCTGGTTTCTGGTCATTGCCAGGCAGGATAAAACGTCGATCAACGCCGGCATGCTCTACTTTTTTATCGCCCACGCCGGATCGGTGCTGATAATGATCGCctt                                                                           <  2:63784/140‑1 (MQ=255)                                                        tCATCGTGCTGTTTGAAATGATATCGCTGTCTTCCTGCTTTCTGGTCATTGCCAGGCAGGATAAAACGTCGATCAACGCCGGCATGCTCTACTTTTTTATCGCCCACGCCGGATCGGTGCTGATAATGATCGCCTTCTTg                                                                       <  1:323668/140‑1 (MQ=255)                                                            cGTGCTGTTTGAAATGATGTCGCTGTCTTCCTGGTTTCTGGTCATTGCCAGGCAGGATAAAACGTCGATCAACGCCGGCATGCTCTACTTTTTTATCGCCCACGCCGGATCGGTGCTGATAATGATCGCCTTCTTGCTGa                                                                   <  2:225437/140‑1 (MQ=255)                                                              tGCTGTTTGAAATGATGTCGCTGTCTTCCTGGTTTCTGGTCATTGCCAGGCAGGATAAAACGTCGATCAACGCCGGCATGCTCTACTTTTTTATCGCCCACGCCGGATCGGTGCTGATAATGATCGCCTTCTTGCTGATg                                                                 >  1:39260/1‑140 (MQ=255)                                                                       aaaTGATGTCGCTGTCCCCCTGCTTTCTGGTCATTCCCAAGCAGGATAAAACGTCGATCAACGCCGGCATGCTCTACTTTTTTATCGCCCACGCCGGATCGGTGCTGATAATGATCGCCTTCTTGCTGATGGGGCGCGaa                                                        <  2:299869/140‑1 (MQ=255)                                                                            atgTCGCTGTCTTCCTGGTTTCTGGTCATTGCCAGGCAGGATAAAACGTCGATCAACGCCGGCATGCTCTACTTTTTTATCGCCCACGCCGGATCGGTGCTGATAATGATCGCCTTCTTGCTGATGGGGCGCGAAAGCgg                                                   <  2:392339/140‑1 (MQ=255)                                                                             tgTCGCTGTCTTCCTGGTTTCTGGTCATTGCCAGGCAGGATAAAACGTCGATCAACGCCGGCATGCTCTACTTTTTTATCGCCCACGCCGGATCGGTGCTGATAATGATCGCCTTCTTGCTGATGGGGCGCGAAAGCGGc                                                  >  1:331289/1‑140 (MQ=255)                                                                                                    gTCATTGCCAGGCAGGATAAAACGTCGATCAACGCCGGCATGCTCTACTTTTTTATCGCCCACGCCGGATCGGTGCTGATAATGATCGCCTTCTTGCTGATGGGGCGCGAAAGCGGCAGCCTCGATTTTGCCAGTTTCCg                           <  2:643991/140‑1 (MQ=255)                                                                                                                caggATAAAACGTCGATCAACGCCGGCATGCTCTACTTTTTTATCGCCCACGCCGGATCGGTGCTGATAATGATCGCCTTCTTGCTGATGGGGCGCGAAAGCGGCAGCCTCGATTTTGCCAGTTTCCGCACGCTTTCACt               <  1:575718/140‑1 (MQ=255)                                                                                                                             cGATCAACGCCGGCATGCTCTACTTTTTTATCGCCCACGCCGGATCGGTGCTGATAATGATCGCCTTCTTGCTGATGGGGCGCGAAAGCGGCAGCCTCGATTTTGCCAGTTTCCGCACGCTTTCACTTTCTCCGGGGCTg  >  1:143884/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                  AATATTTTCATCGCATCGATGGTTGCCCTGCTGGTGATGGACAACGCTTTTTGGTTCATCGTGCTGTTTGAAATGATGTCGCTGTCTTCCTGGTTTCTGGTCATTGCCAGGCAGGATAAAACGTCGATCAACGCTGGCATGCTCTACTTTTTTATCGCCCACGCCGGATCGGTGCTGATAATGATCGCCTTCTTGCTGATGGGGCGCGAAAGCGGCAGCCTCGATTTTGCCAGTTTCCGCACGCTTTCACTTTCTCCGGGGCTG  >  NC_000913/2602172‑2602435

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑