Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,607,878	A→G	N321S (AAC→AGC)	hyfF →	hydrogenase 4 component F

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,607,878	0	A	G	100.0%	97.4 / NA	28	N321S (AAC→AGC)	hyfF	hydrogenase 4 component F
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (18/10);  total (18/10)

TGCCAAGCCATCGGCAGCGATTTCCCCAACCGGTTGTTGCTCATCTTCGGCATGTTGTCGGTTGCCGTGGCGGCATTTTTCATTCTGGTACAGCGGGACATTAAGCGTCTGCTGGCGTACTCCAGCGTGGAGAACATGGGGCTGGTCGCGGTGGAGCTAGGCATTGGCGGGCCGCTGGGAATTTTTGCCGCGCTGCTGCACATCTTAAACCACAGTCTGGCAAAAACGCTGCTGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGG  >  NC_000913/2607745‑2608013                                                                                                                                      |                                                                                                                                        tGCCAAGCCATCGGCAGCGATTTCCCCAACCGGTTGTTGCTCATCTTCGGCATGTTGTCGGTTGCCGTGGCGGCATTTTTCATTCTGGTACAGCGGGACATTTAGCGTCTACTGACGGACTCCATCGTGGAGAGCATggg                                                                                                                                   >  1:285615/1‑140 (MQ=255) tGCCAAGCCATCGGCAGCGATTTCCCCAACCGGTTGTTGCTCATCTTCGGCATGTTGTCGGTTGCCGTGGCGGCATTTTTCATTCTGGTACAGCGGGACATTGAGCGTCTGCTGGCGGACTCCAACGTGGAGAGCATggg                                                                                                                                   >  1:198639/1‑140 (MQ=255)       gCCATCGGCAGCGATTTCCCCATCCGGTTGTTGCTCATCTTCGGCATGTTGTCGGTTGCCGTGGCGGCATTTTTCATTCTGGTACAGCGGGACATTAAGCGTCTGCTGGCGTACTCCAGCGTGGAGAGCATGGGGCTGGt                                                                                                                             <  1:248132/140‑1 (MQ=255)        ccATCGGCAGCGATTTCCCCAACCGGTTGTTGCTCATCTTCGGCATGTTGTCGGTTGCCGTGGCGGCATTTTTCATTCTGGTACAGCGGGACATTAAGCGTCTGCTGGCGTACTCCAGCGTGGAGAGCATGGGGCTGGTc                                                                                                                            <  2:552295/140‑1 (MQ=255)                  cGATTTCCCCAACCGGTTGTTGCTCATCTTCGGCATGTTGTCGGTTGCCGTGGCGGCATTTTTCATTCTGGTACAGCGGGACATTAAGCGTCTGCTGGCGTACTCCAGCGTGGAGAGCATGGGGCTGGTCGCGGTGGAGc                                                                                                                  >  2:72979/1‑140 (MQ=255)                  cGATTTCCCCAACCGGTTGTTGCTCATCTTCGGCATGTTGTCGGTTGCCGTGGCGGCATTTTTCATTCTGGTACAGCGGGACATTAAGCGTCTGCTGGCGTACTCCAGCGTGGAGAGCATGGGGCTGGTCGCGGTGGAGc                                                                                                                  >  2:553162/1‑140 (MQ=255)                                ggTTGTTGCTCATCTTCGGCATGTTGTCGGTTGCCGTGGCGGCATTTTTCATTCTGGTACAGCGGGACATTAAGCGTCTGCTGGCGTACTCCAGCGTGGAGAGCATGGGGCTGGTCGCGGTGGAGCTAGGCATTGGCggg                                                                                                    <  2:113105/140‑1 (MQ=255)                                            tCTTCGGCATGTTGTCGGTTGCCGTGGCGGCATTTTTCATTCTGGTACAGCGGGACATTAAGCGTCTGCTGGCGTACTCCAGCGTGGAGAGCATGGGGCTGGTCGCGGTGGAGCTAGGCATTGGCGGGCCGCTGGGAAtt                                                                                        >  2:126400/1‑140 (MQ=255)                                                       ttgtCGGTTGCCGTGGCGGCATTTTTCATTCTGGTACAGCGGGACATTAAGCGTCTGCTGGCGTACTCCAGCGTGGAGAGCATGGGGCTGGTCGCGGTGGAGCTAGGCATTGGCGGGCCGCTGGGAATTTTTGCCGCgct                                                                             >  2:492063/1‑140 (MQ=255)                                                        tgtCGGTTGCCGTGGCGGCATTTTTCATTCTGGTACAGCGGGACATTAAGCGTCTGCTGGCGTACTCCAGCGTGGAGAGCATGGGGCTGGTCGCGGTGGAGCTAGGCATTGGCGGGCCGCTGGGAATTTTTGCCGCgctg                                                                            <  1:475858/140‑1 (MQ=255)                                                         gtCGGTTGCCGTGGCGGCATTTTTCATTCTGGTACAGCGGGACATTAAGCGTCTGCTGGCGGACTCCAGCGTGGAGAGCATGGGGCTGGTCGCGGTGGAGCTAGGCATTGGCGGGCCGCTGGGAATTTTTGCCGCgctgc                                                                           >  1:363324/1‑140 (MQ=255)                                                             gTTGCCGTGGCGGCATTTTTCATTCTGGTACCGCGTGACCTTAAGCGTATGCTGGCGTACTCCAGCGTGGAGAGCATGGGGCTGGTCGCGGTGGAGCTAGGCATTGGCGGGCCGCTGGGAATTTTTGCCGCGCTGCTGca                                                                       <  2:198639/140‑1 (MQ=255)                                                             gTTGCCGTGGCGGCATTTTTCAGTCTGGTACGGCGGGACATTAAGCGTTTGCTGGCGTACTCCAGCGTGGAGAGCATGGGGCTGGTCGCGGTGGAGCTAGGCATTGGCGGGCCGCTGGGAATTTTTGCCGCGCTGCTGca                                                                       <  2:285615/140‑1 (MQ=255)                                                                gCCGTGGCGGCATTTTTCATTCTGGTACAGCGGGACATTAAGCGTCTGCTGGCGTACTCCAGCGTGGAGAGCATGGGGCTGGTCGCGGTGGAGCTAGGCATTGGCGGGCCGCTGGGAATTTTTGCCGCGCTGCTGcaca                                                                     >  1:19564/1‑139 (MQ=255)                                                                gCCGTGGCGGCATTTTTCATTCTGGTACAGCGGGACATTAAGCGTCTGCTGGCGTACTCCAGCGTGGAGAGCATGGGGCTGGTCGCGGTGGAGCTAGGCATTGGCGGGCCGCTGGGAATTTTTGCCGCGCTGCTGcaca                                                                     <  2:19564/139‑1 (MQ=255)                                                                gCCGTGGCGGCATTTTTCATTCTGGTACAGCGGGACATTAAGCGTCTGCTGGCGTACTCCAGCGTGGAGAGCATGGGGCTGGTCGCGGTGGAGCTAGGCATTGGCGGGCCGCTGGGAATTTTTGCCGCGCTGCTGCACAt                                                                    >  1:466962/1‑140 (MQ=255)                                                                        ggcATTTTTCATTCTGGTACAGCGGGACATTAAGCGTCTGCTGGCGTACTCCAGCGTGGAGAGCATGGGGCTGGTCGCGGTGGAGCTAGGCATTGGCGGGCCGCTGGGAATTTTTGCCGCGCTGCTGCACATCTTAAAcc                                                            >  2:453129/1‑140 (MQ=255)                                                                                        gTACAGCGGGACATTAAGCGTCTGCTGGCGTACTCCAGCGTGGAGAGCATGGGGCTGGTCGCGGTGGAGCTAGGCATTGGCGGGCCGCTGGGAATTTTTGCCGCGCTGCTGCACATCTTAAACCACAGTCTGGCAAAAAc                                            >  2:569369/1‑140 (MQ=255)                                                                                              cGGGACATTAAGCGTCTGCTGGCGTACTCCAGCGTGGAGAGCATGGGGCTGGTCGCGGTGGAGCTAGGCATTGGCGGGCCGCTGGGAATTTTTGCCGCGCTGCTGCACATCTTAAACCACAGTCTGGCAAAAACgctgct                                      >  1:657800/1‑140 (MQ=255)                                                                                              cGGGACATTAAGCGTCTGCTGGCGTACTCCAGCGTGGAGAGCATGGGGCTGGTCGCGGTGGAGCTAGGCATTGGCGGGCCGCTGGGAATTTTTGCCGCGCTGCTGCACATCTTAAACCACAGTCTGGCAAAAACgctgct                                      >  1:456893/1‑140 (MQ=255)                                                                                                ggACATTAAGCGTCTGCTGGCGTACTCCAGCGTGGAGAGCATGGGGCTGGTCGCGGTGGAGCTAGGCATTGGCGGGCCGCTGGGAATTTTTGCCGCGCTGCTGCACATCTTAAACCACAGTCTGGCAAAAACGCTGCTGt                                    >  2:482380/1‑140 (MQ=255)                                                                                                           gTCTGCTGGCGTACTCCAGCGTGGAGAGCATGGGGCTGGTCGCGGTGGAGCTAGGCATTGGCGGGCCGCTGGGAATTTTTGCCGCGCTGCTGCACATCTTAAACCACAGTCTGGCAAAAACGCTGCTGTTCTGCGGTTcc                         >  1:450352/1‑140 (MQ=255)                                                                                                               gctgGCGTACTCCAGCGTGGAGAGCATGGGGCTGGTCGCGGTGGAGCTAGGCATTGGCGGGCCGCTGGGAATTTTTGCCGCGCTGCTGCACATCTTAAACCACAGTCTGGCAAAAACGCTGCTGTTCTGCGGTTCCGGCa                     <  2:167013/140‑1 (MQ=255)                                                                                                               gctgGCGTACTCCAGCGTGGAGAGCATGGGGCTGGTCGCGGTGGAGCTAGGCATTGGCGGGCCGCTGGGAATTTTTGCCGCGCTGCTGCACATCTTAAACCACAGTCTGGCAAAAACGCTGCTGTTCTGCGGTTCCGGCa                     >  1:167013/1‑140 (MQ=255)                                                                                                                     gTACTCCAGCGTGGAGAGCATGGGGCTGGTCGCGGTGGAGCTAGGCATTGGCGGGCCGCTGGGAATTTTTGCCGCGCTGCTGCACATCTTAAACCACAGTCTGGCAAAAACGCTGCTGTTCTGCGGTTCCGGCAATGTAc               <  1:72979/140‑1 (MQ=255)                                                                                                                                tGGAGAGCATGGGGCTGGTCGCGGTGGAGCTAGGCATTGGCGGGCCGCTGGGAATTTTTGCCGCGCTGCTGCACATCTTAAACCACAGTCTGGCAAAAACGCTGCTGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTAc    >  1:590749/1‑140 (MQ=255)                                                                                                                                  gagaGCATGGGGCTGGTCGCGGTGGAGCTAGGCATTGGCGGGCCGCTGGGAATTTTTGCCGCGCTGCTGCACATCTTAAACCACAGTCTGGCAAAAACGCTGCTGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACgg  >  1:645064/1‑140 (MQ=255)                                                                                                                                  gagaGCATGGGGCTGGTCGCGGTGGAGCTAGGCATTGGCGGGCCGCTGGGAATTTTTGCCGCGCTGCTGCACATCTTAAACCACAGTCTGGCAAAAACGCTGCTGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACgg  <  1:492063/140‑1 (MQ=255)                                                                                                                                  gagaGCATGGGGCTGGTCGCGGTGGAGCTAGGCATTGGCGGGCCGCTGGGAATTTTTGCCGCGCTGCTGCACATCTTAAACCACAGTCTGGAAAAAACGCCGCTGTGCCGCGGTTCCAGCAATGTACTGCTCAAGTACgg  >  2:301226/1‑140 (MQ=255)                                                                                                                                      |                                                                                                                                        TGCCAAGCCATCGGCAGCGATTTCCCCAACCGGTTGTTGCTCATCTTCGGCATGTTGTCGGTTGCCGTGGCGGCATTTTTCATTCTGGTACAGCGGGACATTAAGCGTCTGCTGGCGTACTCCAGCGTGGAGAACATGGGGCTGGTCGCGGTGGAGCTAGGCATTGGCGGGCCGCTGGGAATTTTTGCCGCGCTGCTGCACATCTTAAACCACAGTCTGGCAAAAACGCTGCTGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGG  >  NC_000913/2607745‑2608013

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑