| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,610,630 | T→C | V156A (GTC→GCC) | hyfH → | hydrogenase 4 component H |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,610,630 | 0 | T | C | 98.3% | 201.5 / ‑5.0 | 60 | V156A (GTC→GCC) | hyfH | hydrogenase 4 component H |
| Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (14/45); total (15/45) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.50e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01 | |||||||||||
CGTTCCATCTACAACGTTGCAGCCGTTGCGAACGCCCGTTTGCCCCGCAAAAAACCATCGCACTGGCTGCTGAATTGTTAGCACAGCAACAAAATGCGCCACAAAACCGCGAAATGTTGTGGGCGCAAGCGAGCGTCTGCCCGGAATGCAAACAACGCGCGACGCTGATCAACGACGATACAGATGTACTGCTGGTGGCTAAGGAGCAGCTATGAGTCCAGTGCTTACACAACATGTCAGCCAGCCCATCACGCTGGACGAGCAAACGCA > NC_000913/2610495‑2610764 | cGTTCCATCTACAACGTTGCAGCCGTTGCGAACGCCCGTTTGCCCCGCAAAAAACCATCGCACTGGCTGCTGAATTGTTAGCACAGCAACAAAATGCGCCACAAAACCGCGAAATGTTGTGGGCGCAAGCGAGCGCCTGc < 1:436641/140‑1 (MQ=255) ttCCATCTACAACGTTGCAGCCGTTGCGAACGCCCGTTTGCCCCGCAAAAAACCATCGCACTGGCTGCTGAATTGTTAGCACAGCAACAAAATGCGCCACAAAACCGCGAAATGTTGTGGGCGCAAGCGAGCGCCTGccc < 1:38787/140‑1 (MQ=255) ccATCTACAACGTTGCAGCCGTTGCGAACGCCCGTTTGCCCCGCAAAAAACCATCGCACTGGCTGCTGAATTGTTAGCACAGCAACAAAATGCGCCACAAAACCTCGAAATGTTGTGGGCGCAAGCGAGCGCCTGCCCgg < 1:86810/140‑1 (MQ=255) ccATCTACAACGTTGCAGCCGTTGCGAACGCCCGTTTGCCCCGCAAAAAACCATCGCACTGGCTGCTGAATTGTTAGCACAGCAACAAAATGCGCCACAAAACCGCGAAATGTTGTGGGCGCAAGCGAGCGCCTGCCCgg < 1:111396/140‑1 (MQ=255) ccATCTACAACGTTGCAGCCGTCGCAAACGCCCGTTTGCCCCGCAAAAAACCATCGCACTGGCTGCTGAATTGTTAGCACAGCAACAAAATGCGCCACAAAACCGCGAAATGTTGTGGGCGCAAGCGAGCGCCTGCCCgg < 1:217164/140‑1 (MQ=255) aCAACGTTGCAGCCGTTGCGAACGCCCGTTTGCCCCGCAAAAAACCATCGCACTGGCTGCTGAATTGTTAGCACAGCAACAAAATGCGCCACAAAACCGCGAAATGTTGTGGGCGCAAGCGAGCGCCTGCCCGGAATGCa < 2:101338/140‑1 (MQ=255) aCGTTGCAGCCGTTGCGAACGCCCGTTTGCCCCGCAAAAAACCATCGCACTGGCTGCTGAATTGTTAGCACAGCAACAAAATGCGCCACAAAACCGCGAAATGTTGTGGGCGCAAGCGAGCGCCTGCCCGGAATGCAaac > 1:628356/1‑140 (MQ=255) cGTTGCAGCCGTTGCGAACGCCCGTTTGCCCCGCAAAAAACCATCGCACTGGCTGCTGAATTGTTAGCACAGCAACAAAATGCGCCACAAAACCGCGAAATGTTGTGGGCGCAAGCGAGCGCCTGCCCGGAATGCAaaca > 2:447717/1‑140 (MQ=255) ttGCAGCCGTTGCGAACGCCCGTTTGCCCCGCAAAAAACCATCGCACTGGCTGCTGAATTGTTAGCACAGCAACAAAATGCGCCACAAAACCGCGAAATGTTGTGGGCGCAAGCGAGCGCCTGCCCGGAATGCAaacaac > 1:56281/1‑140 (MQ=255) cAGCCGTTGCGAACGCCCGTTTGCCCCGCAAAAAACCATCGCACTGGCTGCTGAATTGTTAGCATAGCAACAAAATGCGCCACAAAACCGCGAAATGTTGTGGGCGCAAGCGAGCGCCTGCCCGGAATGCAAACAAcgcg < 2:442296/140‑1 (MQ=255) cAGCCGTTGCGAACGCCCGTTTGCCCCGCAAAAAACCATCGCACTGGCTGCTGAATTGTTAGCACAGCAACAAAATGCGCCACAAAACCGCGAAATGTTGTGGGCGCAAGCGAGCGCCTGCCCGGAATGCAAACAAcgcg < 1:107211/140‑1 (MQ=255) cAGCCGTTGCGAACGCCCGTTTGCCCCGCAAAAAACCATCGCACTGGCTGCTGAATTGTTAGCACAGCAACAAAATGCGCCACAAAACCGCGAAATGTTGTGGGCGCAAGCGAGCGCCTGCCCGGAATGCAAACAAcgcg > 1:666349/1‑140 (MQ=255) gTTGCGAACGCCCGTTTGCCCCGCAAAAAACCATCGCACTGGCTGCTGAATTGTTAGCACAGCAACAAAATGCGCCACAAAACCGCGAAATGTTGTGGGCGCAAGCGAGCGCCTGCCCGGAATGCAAACAACGCGCGACg < 1:373498/140‑1 (MQ=255) gTTGCGAACGCCCGTTTACCCCCCAAAAAACCATCTCACTGGCTGCTGCATTGTTAGCACAGCAACAAAATGCGCCACAAAACCGCGAAATGTTGTGGGCGCAAGCGAGCGCCTGCCCGGAATGCAAACAACGCGCGACg < 2:183084/140‑1 (MQ=255) aaCGCCCGTTTGCCCCGCAAAAAACCATCGCACTGGCTGCTGAATTGTTAGCACAGCAACAAAATGCGCCACAAAACCGCGAAATGTTGTGGGCGCAAGCGAGCGCCTGCCCGGAATGCAAACAACGCGCGACGCTGATc < 1:365304/140‑1 (MQ=255) cGCCCGTTTGCCCCGCACAAAACCATCGCACGGGCTGCTGAATTGTTATCACAGCAACAAAATGCGCCACAAAACCGCGAAATGTTGTGGGCGCAAGCGAGCGCCTGCCCGGAATGCAAACAACGCGCGACGCTGATCaa < 2:278230/140‑1 (MQ=255) gCCCGTTTGCCCCGCTAAAAACCATCGCACTGGCTGCGGAATTGTTAGCACAGCAACAAAATGCGCCACAAAACCGCGAAATGTTGTGGGCGCAAGCGAGCGCCTGCCCGGAATGCAAACAACGCGCGACGCTGATCAac < 1:206884/140‑1 (MQ=255) gCCCGTTTGCCCCGCAAAAAACCATCGCACTTGCTGCTGGATTGTTAGAACAGCAACAAAATGCGCCACAAAACCGCGAAATGTTGTGGGCGCAAGCGAGCGCCTGCCCGGAATGCAAACAACGCGCGACGCTGATCAac < 2:259936/140‑1 (MQ=255) ccGTTTGCCCCGCAAAACACCATCGCACTGGCTGCAGAATTGTTAGCACAGCAACAAAATGCGCCACAAAACCGCGAAATGTTGTGGGCGCAAGCGAGCGCCTGCCCGGAATGCAAACAACGCGCGACGCTGATCAacga < 2:184584/140‑1 (MQ=255) ccGTTTGCCCCGCAAAAAACCATCGCACTGGCTGCTGAATTGTTAGCACAGCAACAAAATGCGCCACAAAACCGCGAAATGTTGTGGGCGCAAGCGAGCGCCTGCCCGGAATGCAAACAACGCGCGACGCTGATCAacga < 2:355851/140‑1 (MQ=255) ccGTTTGCCCCGCAAAAAACCATCGCACTGGCTGCTGAATTGTTAGCACAGCAACAAAATGCGCCACAAAACCGCGAAATGTTGTGGGCGCAAGCGAGCGCCTGCCCGGAATGCAAACAACGCGCGACGCTGATCAacga < 1:239955/140‑1 (MQ=255) ccGTTTGCCCCGCAAAAAACCATCGCACTGGCTGCTGAATTGTTAGCACAGCAACAAAATGCGCCACAAAACCGCGAAATGTTGTGGGCGCAAGCGAGCGCCTGCCCGGAATGCAAACAACGCGCGACGCTGATCAacga < 1:447717/140‑1 (MQ=255) ccGTTTGCCCCGCAAAAAACCATCGCACTGGCTGCGGAATTGTTAGCACAGCAACAAAATGCGCCACAAAACCGCGAAATGTTGTGGGCGCAAGCGAGCGCCTGCCCGGAATGCAAACAACGCGCGACGCTGATCAacga < 2:232431/140‑1 (MQ=255) tttGCCCCGCAAAAAACCATCGCACTGGCTGCTGTATTGTTAGCACAGCAACAAAATGCGCCACAAAACCGTGAAATGTTGTGGGCGCAAGCGAGCGCCTGCCCGGAATGCAAACAACGCGCGACGCTGATCAAcgacga < 2:286836/140‑1 (MQ=255) tttGCCCCGCAAAAAACCATCGCACTGGCTGCTGAATTGTTAGCACAGCAACAAAATGCGCCACAAAACCGCGAAATGTTGTGGGCGCAAGCGAGCGCCTGCCCGGAATGCAAACAACGCGCGACGCTGATCAAcgacga < 2:628356/140‑1 (MQ=255) cccGCAAAAAACCATCGCACTGGCTGCTGAATTGTTAGCACAGCAACAAAATGCGCCACAAAACCGCGAAATGTTGTGGGCGCAAGCGAGCGCCTGCCCGGAATGCAAACAACGCGCGACGCTGATCAACGACGATACAg < 1:565193/140‑1 (MQ=255) aTCGCACTGGCTGCTGAATTGTTAGCACAGCAACAAAATGCGCCACAAAACCGCGAAATGTTGTGGGCGCAAGCGAGCGCCTGCCCGGAATGCAAACAACGCGCGACGCTGATCAACGACGATACAGATGTACTGCtggt < 2:666349/140‑1 (MQ=255) aTCGCACTGGCTGCTGAATTGTTAGCACAGCAACAAAATGCGCCACAAAACCGCGAAATGTTGTGGGCGCAAGCGAGCGCCTGCCCGGAATGCAAACAACGCGCGACGCTGATCAACGACGATACAGATGTACTGCtggt < 1:341524/140‑1 (MQ=255) aTCGCACTGGCTGCTGAATTGTTAGCACAGCAACAAAATGCGCCACAAAACCGCGAAATGTTGTGGGCGCAAGCGAGCGCCTGCCCGGAATGCAAACAACGCGCGACGCTGATCAACGACGATACAGATGTACTGCtggt < 1:41278/140‑1 (MQ=255) aTCGCACTGGCTGCTGAATTGTTAGCACAGCAACAAAATGCGCCACAAAACCGCGAAATGTTGTGGGCGCAAGCGAGCGCCTGCCCGGAATGCAAACAACGCGCGACGCTGATCAACGACGATACAGATGTACTGCtggt < 1:530591/140‑1 (MQ=255) tGGCTGCTGAATTGTTAGCACAGCAACAAAATGCGCCACAAAACCGCGAAATGTTGTGGGCGCAAGCGAGCGTCTGCCCGGAATGCAAACAACGCGCGACGCTGATCAACGACGATACAGATGTACTGCTGGTGGCTAAg > 1:139763/1‑140 (MQ=255) tGGCTGCTGAATTGTTAGCACAGCAACAAAATGCGCCACAAAACCGCGAAATGTTGTGGGCGCAAGCGAGCGCCTGCCCGGAATGCAAACAACGCGCGACGCTGATCAACGACGATACAGATGTACTGCTGGTGGCTAAg < 2:354930/140‑1 (MQ=255) ggCTGCTGAATTGTTAGCACAGCAACAAAATGCGCCACAAAACCGCGAAATGTTGTGGGCGCAAGCGAGCGCCTGCCCGGAATGCAAACAACGCGCGACGCTGATCAACGACGATACAGATGTACTGCTGGTGGCTAAgg > 2:66051/1‑140 (MQ=255) gctgctGAATTGTTAGCACAGCAACAAAATGCGCCACAAAACCGCGAAATGTTGTGGGCGCAAGCGAGCGCCTGCCCGGAATGCAAACAACGCGCGACGCTGATCAACGACGATACAGATGTACTGCTGGTGGCTAAGGa > 2:468938/1‑140 (MQ=255) ctgctgAATTGTTAGTACAGCAACAAAATGCGCCACATAACCGCGAAATGTTGTGGGCGCAAGCGAGCGCCTGCCCGGAATGCAAACAACGCGCGACGCTGATCAACGACGATACAGATGTACTGCTGGTGGCTAAGGag < 1:258551/140‑1 (MQ=255) ctgctgAATTGTTAGCACAGCAACAAAATTCGCCACAGAACCGCGAAATGTTGTGGGCGCAAGCGAGCGCCTGCCCGGAATGCAAACAACGCGCGACGCTGATCAACGACGATACAGATGTACTGCTGGTGGCTAAGGag < 1:228597/140‑1 (MQ=255) ctgctgAATTGTTAGCACAGCAACAAAATGCGCCACAAAACCGCGAAATGTTGTGGGCGCAAGCGAGCGCCTGCCCGGAATGCAAACAACGCGCGACGCTGATCAACGACGATACAGATGTACTGCTGGTGGCTAAGGag < 2:337773/140‑1 (MQ=255) ctgAATTGTTAGCACAGCAACAAAATGCGCCACAAAACCGCGAAATGTTGTGGGCGCAAGCGAGCGCCTGCCCGGAATGCAAACAACGCGCGACGCTGATCAACGACGATACAGATGTACTGCTGGTGGCTAAGGagcag > 2:519752/1‑140 (MQ=255) aTTGTTAGCACAGCAACAAAATGCGCCACAAAACCGCGAAATGTTGTGGGCGCAAGCGAGCGCCTGCCCGGAATGCAAACAACGCGCGACGCTGATCAACGACGATACAGATGTACTGCTGGTGGCTAAGGAGCAGCTAt < 2:389976/140‑1 (MQ=255) aTTGTTAGCACAGCAACAAAATGCGCCACAAAACCGCGAAATGTTGTGGGCGCAAGCGAGCGCCTGCCCGGAATGCAAACAACGCGCGACGCTGATCAACGACGATACAGATGTACTGCTGGTGGCTAAGGAGCAGCTAt < 2:518633/140‑1 (MQ=255) aTTGTTAGCACAGCAACAAAATGCGCCACAAAACCGCGAAATGTTGTGGGCGCAAGCGAGCGCCTGCCCGGAATGCAAACAACGCGCGACGCTGATCAACGACGATACAGATGTACTGCTGGTGGCTAAGGAGCAGCTAt < 1:468938/140‑1 (MQ=255) tAGCACAGCAACAAACTGCGCCGCATAACCGCGAAATTTTGTGGGCGCAAGCGAGCGCCTGCCCGGAATGCAAACAACGCGCGACGCTGATCAACGACGATACAGATGTACTGCTGGTGGCTAAGGAGCAGCTATGAGTc < 1:316610/140‑1 (MQ=255) tAGCACAGCAACAAAATGCGCCACAAAACCGCGAAATGTTGTGGGCGCAAGCGAGCGCCTGCCCGGAATGCAAACAACGCGCGACGCTGATCAACGACGATACAGATGTACTGCTGGTGGCTAAGGAGCAGCTATGAGTc < 1:519752/140‑1 (MQ=255) aGCACAGCAACAAAATGCGCCACAAAACCGCGAAATGTTGTGGGCGCAAGCGAGCGCCTGCCCGGAATGCAAACAACGCGCGACGCTGATCAACGACGATACAGATGTACTGCTGGTGGCTAAGGAGCAGCTATGAGTcc > 1:500000/1‑140 (MQ=255) acaGCAACAAAATGCGCGACAAAACCGCGAATTGTTGTGGGCGCAAGCGAGCGCCTGCCCGGAATGCAAACAACGCGCGACGCTGATCAACGACGATACAGATGTACTGCTGGTGGCTAAGGAGCAGCTATGAGTCCAGt < 2:278631/140‑1 (MQ=255) acaGCAACAAAATGCGCCACAAAACCGCGAAATGTTGTGGGCGCAAGCGAGCGCCTGCCCGGAATGCAAACAACGCGCGACGCTGATCAACGACGATACAGATGTACTGCTGGTGGCTAAGGAGCAGCTATGAGTCCAGt < 1:486028/140‑1 (MQ=255) acaGCAACAAAATGCGCCACAAAACCGCGAAATGTTGTGGGCGCAAGCGAGCGCCTGCCCGGAATGCAAACAACGCGCGACGCTGATCAACGACGATACAGATGTACTGCTGGTGGCTAAGGAGCAGCTATGAGTCCAGt < 1:154574/140‑1 (MQ=255) aGCAACAAAATGCGCCACAAAACCGCGAAATGTTGTGGGCGCAAGCGAGCGCCTGCCCGGAATGCAAACAACGCGCGACGCTGATCAACGACGATACAGATGTACTGCTGGTGGCTAAGGAGCAGCTATGAGTCCAGTGc > 2:582824/1‑140 (MQ=255) aacaaAATGCGCCACAAAACCGCGAAATGTTGTGGGCGCAAGCGAGCGCCTGCCCGGAATGCAAACAACGCGCGACGCTGATCAACGACGATACAGATGTACTGCTGGTGGCTAAGGAGCAGCTATGAGTCCAGTGCTTa < 1:582003/140‑1 (MQ=255) caaAATGCGCCACAAAACCGCGAAATGTTGTGGGCGCAAGCGAGCGCCTGCCCGGAATGCAAACAACGCGCGACGCTGATCAACGACGATACAGATGTACTGCTGGTGGCTAAGGAGCAGCTATGAGTCCAGTGCTTaca < 1:12491/140‑1 (MQ=255) gcgcCACAAAACCGCGAAATGTTGTGGGCGCAAGCGAGCGCCTGCCCGGAATGCAAACAACGCGCGACGCTGATCAACGACGATACAGATGTACTGCTGGTGGCTAAGGAGCAGCTATGAGTCCAGTGCTTACACAACAt < 1:253022/140‑1 (MQ=255) cgcCACAAAACCGCGAAATGTTGTGGGCGCAAGCGAGCGCCTGCCCGGAATGCAAACAACGCGCGACGCTGATCAACGACGATACAGATGTACTGCTGGTGGCTAAGGAGCAGCTATGAGTCCAGTGCtt > 2:76849/1‑130 (MQ=255) cgcCACAAAACCGCGAAATGTTGTGGGCGCAAGCGAGCGCCTGCCCGGAATGCAAACAACGCGCGACGCTGATCAACGACGATACAGATGTACTGCTGGTGGCTAAGGAGCAGCTATGAGTCCAGTGCtt < 1:76849/130‑1 (MQ=255) cgcCACAAAACCGCGAAATGTTGTGGGCGCAAGCGAGCGCCTGCCCGGAATGCAAACAACGCGCGACGCTGATCAACGACGATACAGATGTACTGCTGGTGGCTAAGGAGCAGCTATGAGTCCAGTGCTTACACAACATg > 2:509554/1‑140 (MQ=255) cgcCACAAAACCGCGAAATGTTGTGGGCGCAAGCGAGCGCCTGCCCGGAATGCAAACAACGCGCGACGCTGATCAACGACGATACAGATGTACTGCTGGTGGCTAAGGAGCAGCTATGAGTCCAGTGCTTACACAACATg > 2:399801/1‑140 (MQ=255) caAAACCGCGAAATGTTGTGGGCGCAAGCGAGCGCCTGCCCGGAATGCAAACAACGCGCGACGCTGATCAACGACGATACAGATGTACTGCTGGTGGCTAAGGAGCAGCTATGAGTCCAGTGCTTACACAACATGTcagc < 2:56281/140‑1 (MQ=255) cgAAATGTTGTGGGCGCAAGCGAGCGCCTGCCCGGAATGCAAACAACGCGCGACGCTGATCAACGACGATACAGATGTACTGCTGGTGGCTAAGGAGCAGCTATGAGTCCAGTGCTTACACAACATGTCAGCCAGCCCAt > 2:151455/1‑140 (MQ=255) aTGTTGTGGGCGCAAGCGAGCGCCTGCCCGGAATGTAAATAACGCGCGTCGCTGATCAACGACGATACAGATGTACTGCTGGTGGCTAAGGAGCAGCTATGAGTCCAGTGCTTACACAACATGTCAGCCAGCCCATCACg < 2:282229/140‑1 (MQ=255) ggCGCAAGCGAGCGCCTGCCCGGAATGCAAACAACGCGCGACGCTGATCAACGACGATACAGATGTACTGCTGGTGGCTAAGGAGCAGCTATGAGTCCAGTGCTTACACAACATGTCAGCCAGCCCATCACGCTGGACGa > 1:74242/1‑140 (MQ=255) gagcgCCTGCCCGGAATGCAAACAACGCGCGACGCTGATCAACGACGATACAGATGTACTGCTGGTGGCTAAGGAGCAGCTATGAGTCCAGTGCTTACACAACATGTCAGCCAGCCCATCACGCTGGACGAGCAAACGCa < 2:74242/140‑1 (MQ=255) | CGTTCCATCTACAACGTTGCAGCCGTTGCGAACGCCCGTTTGCCCCGCAAAAAACCATCGCACTGGCTGCTGAATTGTTAGCACAGCAACAAAATGCGCCACAAAACCGCGAAATGTTGTGGGCGCAAGCGAGCGTCTGCCCGGAATGCAAACAACGCGCGACGCTGATCAACGACGATACAGATGTACTGCTGGTGGCTAAGGAGCAGCTATGAGTCCAGTGCTTACACAACATGTCAGCCAGCCCATCACGCTGGACGAGCAAACGCA > NC_000913/2610495‑2610764 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |