Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,656,200	A→G	G53G (GGT→GGC)	pepB ←	aminopeptidase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,656,200	0	A	G	100.0%	132.2 / NA	38	G53G (GGT→GGC)	pepB	aminopeptidase B
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (12/26);  total (12/26)

GGCGCTGGGCATCGTCCAGATCCGGCCACACCACTTTACGCGTGCCTTTCGGGGCTTTGTAACCTTGCCAGAATGCCCAGCAGCGATCCGCATCCCAGCCTTCACCGCTTAACTGAACATGCTTGATGCCCAGACCGTCAATCTTGCGCGCCGCACGCTGGATCAGCCCCAGATCGTCTGCCCCGTTCAAATGCAGGGTAATGCCGTCATTATTAATGCTGTAAGTTGCTTTTTCTCCCCAGCGCGCGTCGGCAGGTTGGGTAGA  >  NC_000913/2656067‑2656331                                                                                                                                      |                                                                                                                                    ggCGCTGGGCATCGTCCAGATCCGGCCACACCACTTTACGCGTGCCTTTCGGGGCTTTGTAACCTTGCCAGAATGCCCAGCAGCGATCCGCATCCCAGCCTTCACCGCTTAACTGAACATGCTTGATGCCCAGGCCGTCa                                                                                                                               >  1:460183/1‑140 (MQ=255)              gTCCAGATCCGGCCACACCACTTTACGCGTGCCTTTCGGGGCTTTGTAACCTTGCCAGAATGCCCAGCAGCGATCCGCATCCCAGCCTTCACCGCTTAACTGAACATGCTTGATGCCCAGGCCGTCAATCTTGCGcgccg                                                                                                                  <  2:437341/140‑1 (MQ=255)              gTCCAGATCCGGCCACACCACTTTACGCGTGCCTTTCGGGGCTTTGTAACCTTGCCAGAATGCCCAGCAGCGATCCGCATCCCAGCCTTCACCGCTTAACTGAACATGCTTGATGCCCAGGCCGTCAATCTTGCGcgccg                                                                                                                  <  1:473305/140‑1 (MQ=255)               tCCAGATCCGGCCACACCACTTTGCGCGTGCCTTTCGGGGCTTTGTAACCTTGCCAGAATGCCCAGCAGCGATCCGCATCCCAGCCTTCACCGCTTAACTGAACATGCTTGATGCCCAGGCCGTCAATCTTGCGcgcc                                                                                                                   <  2:606476/138‑1 (MQ=255)               tCCAGATCCGGCCACACCACTTTGCGCGTGCCTTTCGGGGCTTTGTAACCTTGCCAGAATGCCCAGCAGCGATCCGCATCCCAGCCTTCACCGCTTAACTGAACATGCTTGATGCCCAGGCCGTCAATCTTGCGcgcc                                                                                                                   >  1:606476/1‑138 (MQ=255)                   gATCCGGCCACACCACTTTACGCGTGCCTTTCGGGGCTTTGTAACCTTGCCAGAATGCCCAGCAGCGATCCGCATCCCAGCCTTCACCGCTTAACTGAACATGCTTGATGCCCAGGCCGTCAATCTTGCGCGCCGCACGc                                                                                                             <  2:645203/140‑1 (MQ=255)                   gATCCGGCCACACCACTTTACGCGTGCCTTTCGGGGCTTTGTAACCTTGCCAGAATGCCCAGCAGCGATCCGCATCCCAGCCTTCACCGCTTAACTGAACATGCTTGATGCCCAGGCCGTCAATCTTGCGCGCCGCACGc                                                                                                             >  1:120328/1‑140 (MQ=255)                       cGGCCACACCACTTTACGCGTGCCTTTCGGGGCTTTGTAACCTTGCCAGAATGCCCAGCAGCGATCCGCATCCCAGCCTTCACCGCTTAACTGAACATGCTTGATGCCCAGGCCGTCAATCTTGCGCGCCGCACGCTGGa                                                                                                         <  1:616432/140‑1 (MQ=255)                        ggCCACACCACTTTATGCGTGCCTTACGGCGCTTTGTAACCTTGCCAGAATGCCCAGCAGCGATCCGCATCCCAGCCTTCACCGCTTAACTGAACATGCTTGATGCCCAGGCCGTCAATCTTGCGCGCCGCACGCTGGAt                                                                                                        <  1:280634/140‑1 (MQ=255)                        ggCCACACCACTTTATGCGTGCATTGCGGAGCTTTGTAACCTTGCCAGAATGCCCAGCAGCGATCCGCATCCCAGCCTTCACCGCTTAACTGAACATGCTTGATGCCCAGGCCGTCAATCTTGCGCGCCGCACGCTGGAt                                                                                                        <  1:316741/140‑1 (MQ=255)                        ggCCACACCACTTTACGCGTGCCTTTCGGGGCTTTGTAACCTTGCCAGAATGCCCAGCAGCGATCCGGATCCCAGCCTTCACCGCTTAACTGAACATGCTTGATGCCCAGGCCGTCAATCTTGCGCGCCGCACGCTGGAt                                                                                                        <  1:60542/140‑1 (MQ=255)                        ggCCACACCACTTTACGCGTGCCTTTCGGGGCTTTGTAACCTTGCCAGAATGCCCAGCAGCGATCCGCATCCCAGCCTTCACCGCTTAACTGAACATGCTTGATGCCCAGGCCGTCAATCTTGCGCGCCGCACGCTGGAt                                                                                                        <  2:524618/140‑1 (MQ=255)                        ggCCACACCACTTTACGCGTGCCTTTCGGGGCTTTGTAACCTTGCCAGAATGCCCAGCAGCGATCCGCATCCCAGCCTTCACCGCTTAACTGAACATGCTTGATGCCCAGGCCGTCAATCTTGCGCGCCGCACGCTGGAt                                                                                                        <  2:126673/140‑1 (MQ=255)                        ggCCACACCACTTTACGCGTGCCTTTCGGGGCTTTGTAACCTTGCCAGAATGCCCAGCAGCGATCCGCATCCCAGCCTTCACCGCTTAACTGAACATGCTTGATGCCCAGGCCGTCAATCTTGCGCGCCGCACGCTGGAt                                                                                                        <  1:71333/140‑1 (MQ=255)                        ggCCACACCACTTTACGCGTGCCTTTCGGGGCTTTGTAACCTTGCCAGAATGCCCAGCAGCGATCCGCATCCCAGCCTTCACCGCTTAACTGAACATGCTTGATGCCCAGGCCGTCAATCTTGCGCGCCGCACGCTGGAt                                                                                                        <  1:659538/140‑1 (MQ=255)                            ccaccacTTTACGCGTGCCTTTCGGGGCTTTGTAACCTTGCCAGAATGCCCAGCAGCGATCCGCATCCCAGCCTTCACCGCTTAACTGAACATGCTTGATGCCCAGGCCGTCAATCTTGCGCGCCGCACGCTGGATCAGc                                                                                                    <  1:41305/139‑1 (MQ=255)                                        gcgTGCCTTTCGGGGCTTTGTAACCTTGCCAGAATGCCCAGCAGCGATCCGCATCCCAGCCTTCACCGCTTAACTGAACATGCTTGATGCCCAGGCCGTCAATCTTGCGCGCCGCACGCTGGATCAGCCCCAGATCGTCt                                                                                        >  2:154671/1‑140 (MQ=255)                                                      gCTTTGTAACCTTGCCAGAATGCCCAGCAGCGATCCGCATCCCAGCCTTCACCGCTTAACTGAACATGCTTGATGCCCAGGCCGTCAATCTTGCGCGCCGCACGCTGGATCAGCCCCAGATCGTCTGCCCCGTTCAAATg                                                                          <  1:22792/140‑1 (MQ=255)                                                      gCTTTGTAACCTTGCCAGAATGCCCAGCAGCGATCCGCATCCCAGCCTTCACCGCTTAACTGAACATGCTTGATGCCCAGGCCGTCAATCTTGCGCGCCGCACGCTGGATCAGCCCCAGATCGTCTGCCCCGTTCAAATg                                                                          <  2:460183/140‑1 (MQ=255)                                                            tAACCTTGCCAGAATCCCCAGCTGCTATCAGCATCCCCGCCTTCACCGCTTAACTGAACATGCTTGATGCCCAGGCCGTCAATCTTGCGCGCCGCACGCTGGATCAGCCCCAGATCGTCTGCCCCGTTCAAATGCAGGGt                                                                    <  1:195991/140‑1 (MQ=255)                                                               ccTTGCCAGAATGCCCAGCAGCGATCCGCATCCCAGCCTTCACCGCTTAACTGAACATGCTTGATGCCCAGGCCGTCAATCTTGCGCGCCGCACGCTGGATCAGCCCCAGATCGTCTGCCCCGTTCAAATGCAGGGTAAt                                                                 <  2:47550/140‑1 (MQ=255)                                                               ccTTGCCAGAATGCCCAGCAGCGATCCGCATCCCAGCCTTCACCGCTTAACTGAACATGCTTGATGCCCAGGCCGTCAATCTTGCGCGCCGCACGCTGGATCAGCCCCAGATCGACTGCCCCGTTCAAATGCAGGGTAAt                                                                 <  1:588502/140‑1 (MQ=255)                                                                     cAGAATGCCCAGCAGTGATCCGGATGCCAGCCTTCACCGCTTAACTGAACATGCTTGATGCCCAGGCCGTCAATCTTGCGCGCCGCACGCTGGATCAGCCCCAGATCGTCTGCCCCGTTCAAATGCAGGGTAATGCCGTc                                                           <  1:274349/140‑1 (MQ=255)                                                                     cAGAATGCCCAGCAGCGATCCGCATCCCAGCCTTCACCGCTTAACTGAACATGCTTGATGCCCAGGCCGTCAATCTTGCGCGCCGCACGCTGGATCAGCCCCAGATCGTCTGCCCCGTTCAAATGCAGGGTAATGCCGTc                                                           <  2:480291/140‑1 (MQ=255)                                                                     cAGAATGCCCAGCAGCGATCCGCATCCCAGCCTTCACCGCTTAACTGAACATGCTTGATGCCCAGGCCGTCAATCTTGCGCGCCGCACGCTGGATCAGCCCCAGATCGTCTGCCCCGTTCAAATGCAGGGTAATGCCGTc                                                           <  1:510008/140‑1 (MQ=255)                                                                     cAGAATGCCCAGCAGCGATCCGCATCCCAGCCTTCACCGCTTAACTGAACATGCTTGATGCCCAGGCCGTCAATCTTGCGCGCCGCACGCTGGATCAGCCCCAGATCGTCTGCCCCGTTCAAATGCAGGGTAATGCCGTc                                                           <  2:354299/140‑1 (MQ=255)                                                                             ccAGCAGCGATCCGCATCCCAGCCTTCACCGCTTAACTGAACATGCTTGATGCCCAGGCCGTCAATCTTGCGCGCCGCACGCTGGATCAGCCCCAGATCGTCTGCCCCGTTCAAATGCAGGGTAATGCCGTCATTATTaa                                                   <  1:598980/140‑1 (MQ=255)                                                                                 cagcGATCCGCATCCCAGCCTTCACCGCTTAACTGAACATGCTTGATGCCCAGGCCGTCAATCTTGCGCGCCGCACGCTGGATCAGCCCCAGATCGTCTGCCGCGTTCAAATGCGGGTTAATGCTGTCATTATTAATGCt                                               >  1:302325/1‑140 (MQ=255)                                                                                      aTCCGCATCCCAGCCTTCACCGCTTAACTGAACATGCTTGATGCCCAGGCCGTCAATCTTGCGCGCCGCACGCTGGATCAGCCCCAGATCGTCTGCCCCGTTAAAATGCAGGGTCATTCCGTCAGTATTAATGCTGTAAg                                          >  1:279327/1‑140 (MQ=255)                                                                                          gCATCCCAGCCTTCACCGCTTAACTGAACATGCTTGATGCCCAGGCCGTCAATCTTGCGCGCCGCACGCTGGATCAGCCCCAGATCGTCTGCCCCGTTCAAATGCAGGGTAATGCCGTCATTATTAATGCTGTAAGTTGc                                      <  2:337351/140‑1 (MQ=255)                                                                                                        aCCGCTTAACTGAACATGCTTGATGCCCAGGCCGTCAATCTTGCGCGCCGCACGCTGGATCAGCCCCAGATCGTCTGCCCCGTTCAAATGCAGGGTAATGCCGTCATTATTAATGCTGTAAGTTGCTTTTTCTCCCCAgc                        >  1:633147/1‑140 (MQ=255)                                                                                                                 cTGAACATGCTTGATGAGCAGGCCGTCAATCCTACACGCCGCACGCTGGATCAGCCCCAGATCGTCTGCCCCGTTCAAATGCAGGGTAATGCCGTCATTATTAATGCTGTAAGTTGCTTTTTCTCCCCAGCGCGCGTCgg               <  2:302325/140‑1 (MQ=255)                                                                                                                  tGAACATGCTTGATGCTCAGGCCGTCAATCTTGCGCGCCGCACGCTGGATCAGCCCCAGATCGTCTGCCCCGTTCAAATGCAGGGTAATGCCGTCATTATTAATGCTGTAAGTTGCTTTTTCTCCCCAGCGCGCGTCGGc              <  2:120328/140‑1 (MQ=255)                                                                                                                    aaCATGCTTGATGCCCAGGCCGTCAATCTTGCGCGCCGCACGCTGGATCAGCCCCAGATCGTCTGCCCCGTTCAAATGCAGGGTAATGCCGTCATTATTAATGCTGTAAGTTGCTTTTTCTCCCCAGCGCGCGTCGGCAg            >  1:363815/1‑140 (MQ=255)                                                                                                                      cATGCTTGATGCCCAGGCCGTCAATCTTGCGCGCCGCACGCTGGATCAGCCCCAGATCGTCTGCCCCGTTCAAATGCAGGGTAATGCCGTCATTATTAATGCTGTAAGTTGCTTTTTCTCCCCAGCGCGCGTCGGCAGGt          >  1:596196/1‑140 (MQ=255)                                                                                                                         gCTTGATGCCCAGGCCGTCAATCTTGCGCGCCGCACGCTGGATCAGCCCCAGATCGTCTGCCCCGTTCAAATGCAGGGTAATGCCGTCATTATTAATGCTGTAAGTTGCTTTTTCTCCCCAGCGCGCGTCGGCAGGTTgg       >  2:12456/1‑140 (MQ=255)                                                                                                                           ttGATGCCCAGGCCGTCAATCTTGCGCGCCGCACGCTGGATCAGCCCCAGATCGTCTGCCCCGTTCAAATGCAGGGTAATGCCGTCATTATTAATGCTGTAAGTTGCTTTTTCTCCCCAGCGCGCGTCGGCAGGTTGGGt     <  2:51407/140‑1 (MQ=255)                                                                                                                              aTGCCCAGGCCGTCAATCTTGCGCGCCGCACGCTGGATCAGCCCCAGATCGTCTGCCCCGTTCAAATGCAGGGTAATGCCGTCATTATTAATGCTGTAAGTTGCTTTTTCTCCCCAGCGCGCGTCGGCAGGTTGGGTaga  >  1:595905/1‑140 (MQ=255)                                                                                                                              aTGCCCAGGCCGTCAATCTTGCGCGCCGCACGCTGGATCAGCCCCAGATCGTCTGCCCCGTTCAAATGCAGGGTAATGCCGTCATTATTAATGCTGTAAGTTGCTTTTTCTCCCCAGCGCGCGTCGGCAGGTTGGGTaga  >  1:363004/1‑140 (MQ=255)                                                                                                                                      |                                                                                                                                    GGCGCTGGGCATCGTCCAGATCCGGCCACACCACTTTACGCGTGCCTTTCGGGGCTTTGTAACCTTGCCAGAATGCCCAGCAGCGATCCGCATCCCAGCCTTCACCGCTTAACTGAACATGCTTGATGCCCAGACCGTCAATCTTGCGCGCCGCACGCTGGATCAGCCCCAGATCGTCTGCCCCGTTCAAATGCAGGGTAATGCCGTCATTATTAATGCTGTAAGTTGCTTTTTCTCCCCAGCGCGCGTCGGCAGGTTGGGTAGA  >  NC_000913/2656067‑2656331

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑