Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,692,928	C→T	A872A (GCG→GCA)	purL ←	phosphoribosylformylglycinamide synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,692,928	0	C	T	100.0%	129.6 / NA	39	A872A (GCG→GCA)	purL	phosphoribosylformylglycinamide synthetase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (17/22);  total (17/22)

CCAGGCGATCGTCACCCAGAGTGGCGATATCCGCGTCAATGCCACAATGACCAGCAAAGGCCATTTCCGCCAGCGTTACCAGCAGGCCGCCATCAGAGCGGTCGTGATACGCCAGCAGCTTACGCTGTGCAACCAGCGCCTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAAT  >  NC_000913/2692792‑2693059                                                                                                                                         |                                                                                                                                    ccAGGCGATCGTCACCCAGAGTGGCGATATCCGCGTCAATGCCACAATGACCAGCAAAGGCCATTTCCGCCAGCGTTACCAGCAGGCCGCCGTCAGAGCGGTCGGGATACGCCAGCAGCTTAGGCTGTGCAACCAGTGcc                                                                                                                                  >  2:278765/1‑140 (MQ=255)         tCGTCACCCAGAGTGGCGATATCCGCGTCAATGCCACAATGACCAGCAAAGGCCATTTCCGCCAGCGTTACCAGCAGGCCGCCATCAGAGCGGTCGTGATACGCCAGCAGCTTACGCTGTGCAACCAGTGCCTGAATcgc                                                                                                                          <  2:536762/140‑1 (MQ=255)          cGTCACCCAGAGTGGCGATATCCGCGTCAATGCCACAATGACCAGCAAAGGCCATTTCCGCCAGCGTTACCAGCAGGCCGCCATCAGAGCGGTCGTGATACGCCAGCAGCTTACGCTGTGCAACCAGTGCCTGAATcgcg                                                                                                                         <  2:65967/140‑1 (MQ=255)              aCCCAGAGTGGCGATATCCGCGTCAATGCCACAATGACCAGCAAAGGCCATTTCCGCCAGCGTTACCAGCATGCCGCCATCAGCGCGGTCGTGATACGCCAGCAGCTTACGCTGTGCAACCAGTGCCTGAAGCGCGTCAt                                                                                                                     <  1:587662/140‑1 (MQ=255)                 cAGAGTGGCGATATCGGCGTCATTGTCACTATGACCATCAAAGGCCATTTCCGCCAGCGTTACCAGCAGGCCGCCATCAGAGCGGTCGTGATACGCCAGCAGCTTACGCTGTGCAACCAGTGCCTGAATCGCGTCATAGa                                                                                                                  <  1:324323/140‑1 (MQ=255)                 cAGAGTGGCGATATCCGCGTCAGTGCCACAAAGACCAGCAAAGGCCATTTCCGCCAGCGTTACCAGCAGGCCGCCATCAGAGCGGTCGTGATACGCCAGCAGCTTACGCTGTGCAACCAGTGCCTGAATCGCGTCATAGa                                                                                                                  <  2:231851/140‑1 (MQ=255)                    agTGGCGATATCCGCGTCAATGCCACAATGACCAGCAAAGGCCATTTCCGCCAGCGTTACCAGCAGGCCGCCATCAGAGCGGTCGTGATACGCCAGCAGCTTACGCTGTGCAACCAGTGCCTGAATCGCGTCATAGAAGc                                                                                                               >  1:118225/1‑140 (MQ=255)                       ggCGATATCCGCGTCAATGCCACAATGACCAGCAAAGGCCATTTCCGCCAGCGTTACCAGCAGGCCGCCATCAGAGCGGTCGTGATACGCCAGCAGCTTACGCTGTGCAACCAGTGCCTGAATCGCGTCATAGAAGCCtt                                                                                                            >  2:555825/1‑140 (MQ=255)                                  cgTCAATGCCACAATGACCAGCAAAGGCCATTTCCGCCAGCGTTACCAGCAGGCCGCCATCAGAGCGGTCGTGATACGCCAGCAGCTTACGCTGTGCAACCAGTGCCTGAATCGCGTCATAGAAGCCTTTCAGTTGcgcg                                                                                                 <  1:338181/140‑1 (MQ=255)                                   gTCAATGCCACAATGACCAGCAAAGGCCATTTCCGCCAGCGTTACCAGCAGGCCGCCATCAGAGCGGTCGTGATACGCCAGCAGCTTACGCTGTGCAACCAGTGCCTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGa                                                                                                <  1:339911/140‑1 (MQ=255)                                   gTCAATGCCACAATGACCAGCAAAGGCCATTTCCGCCAGCGTTACCAGCAGGCCGCCATCAGAGCGGTCGTGATACGCCAGCAGCTTACGCTGTGCAACCAGTGCCTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGa                                                                                                >  1:644999/1‑140 (MQ=255)                                     cAATGCCACAATGACCAGACAAGGTCCTTTCCGGCAGCGTTAACAGCAGGACGACATCAGAGCGGTCGTGATACGGCAGTAGCTTACGCTTTGGTATTAGTGCCTGAAGCGCTTAATAGAAGGATATCAGTTGCGCGACa                                                                                              <  2:664928/140‑1 (MQ=255)                                      aaTGCCACAATGACCAGCAAAGGCCATTTCCGCCAGCGTTACCAGCAGGCCGCCATCAGAGCGGTCGTGATACGCCAGCAGCTTACGCTGTGCAACCAGTGCCTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACAt                                                                                             >  1:664928/1‑140 (MQ=255)                                           cacaATGACCAGCAAAGGCCATCTCCGCCTGCGTTACCAGCAGGCCGCCATCAGAGCGGTCGTGATACGCCAGCAGCTTACGCTGTGCAACCAGTGCCTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGt                                                                                        <  1:230220/140‑1 (MQ=255)                                                          aGGCCATTTCCGCCAGCGTTACCAGCAGGCCGCCATCAGAGCGGTCGTGATACGCCAGCAGCTTACGCTGTGCAACCAGTGCCTGAATCGCGTCATAGAAGCTTTTCAGTTGCGGGAGATCGCGTACATCTGCCGGTTTg                                                                         >  1:227826/1‑140 (MQ=255)                                                            gCCATTTCCGCCAGCGTTACCAGCAGGCCGCCATCAGAGCGGTCGTGATACGCCAGCAGCTTACGCTGTGCAACCAGTGCCTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTc                                                                       >  1:106362/1‑140 (MQ=255)                                                            gCCATTTCCGCCAGCGTTACCAGCAGGCCGCCATCAGAGCGGTCGTGATACGCCAGCAGCTTACGCTGTGCAACCAGTGCCTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTc                                                                       >  1:20712/1‑140 (MQ=255)                                                               aTTTCCGCCAGCGTTACCAGCAGGCCGCCATCAGAGCGGTCGTGATACGCCAGCAGCTTACGCTGTGCAACCAGTGCCTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGcc                                                                    <  1:555825/140‑1 (MQ=255)                                                               aTTTCCGCCAGCGTTACCAGCAGGCCGCCATCAGAGCGGTCGTGATACGCCAGCAGCTTACGCTGTGCAACCAGTGCCTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGcc                                                                    <  2:20712/140‑1 (MQ=255)                                                                 ttCCGCCAGCGTTACCAGCAGGCCGCCATCAGAGCGGTCGTGATACGCCAGCAGCTTACGCTGTGCAACCAGTGCCTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCaa                                                                  >  1:68091/1‑140 (MQ=255)                                                                   ccGCCAGCGTTACCAGCAGGCCTCCATCAGATCGGTCGTAATACGCCACCAGCTTACGCTGTGCAACCAGTGCCTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGc                                                                <  2:254240/140‑1 (MQ=255)                                                                    cGCCAGCGTTACCAGCAGGCCGCCATCAGAGCGGTCGTGATACGCCAGCAGCTTACGCTGTGCAACCAGTGCCTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCt                                                               <  1:396326/140‑1 (MQ=255)                                                                    cGCCAGCGTTACCAGCAGGCCGCCATCAGAGCGGTCGTGATACGCCAGCAGCTTACGCTGTGCAACCAGTGCCTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCt                                                               <  1:514729/140‑1 (MQ=255)                                                                    cGCCAGCGTTACCAGCAGGCCGCCATCAGAGCGGTCGTGATACGCCAGCAGCTTACGCTGTGCAACCAGTGCCTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCt                                                               <  2:106362/140‑1 (MQ=255)                                                                                    agGCCGCCATCAGAGCGGTCGTGATACGCCAGCAGCTTACGCTGTGCAACCAGTGCCTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTgcgc                                               >  1:356177/1‑140 (MQ=255)                                                                                       ccgccATCAGAGCGGTCGTGATACGCCAGCAGCTTACGCTGTGCAACCAGTGCCTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATGGCGTACATATGCCGCTGTGTCGCCAGGCTGACGATAAACCTGCGCCAg                                            >  2:301676/1‑140 (MQ=255)                                                                                               agagCGGTCGTGATATGCCAGCCGCATACCCTGTGCACCCAGTGCCTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTg                                    <  1:198417/140‑1 (MQ=255)                                                                                                gagCGGTCGTGATACGCCAGCAGCTTACGCTGTGCAACCAGTGCCTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTgc                                   >  1:360767/1‑140 (MQ=255)                                                                                                     gTCGTGATACGCCAGCAGCTTACGCTGTGCAACCAGTGCCTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACAATAAACATGCGCCGGCGCCGTTGCGCCCa                              >  1:181759/1‑140 (MQ=255)                                                                                                      tCGTGATACGCCAGCAGCTTACGCTGTGCAACCAGTGCCTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAg                             <  2:581541/140‑1 (MQ=255)                                                                                                       cGTGATACGCCAGCAGCTTACGCTGCGCAACCAGTGCGTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAgc                            <  1:226230/140‑1 (MQ=255)                                                                                                        gTGATACGCCAGCAGCTTACGCTGTGCAACCAGTGCCTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAgcg                           <  2:617017/140‑1 (MQ=255)                                                                                                                ccAGCAGCTTACGCTGTGCAACCAGTGCCTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTg                   >  1:143741/1‑140 (MQ=255)                                                                                                                ccAGCAGCTTACGCTGTGCAACCAGTGCCTGAATCGCGTCATAGAAGCCTTTCAGTGGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTg                   >  1:490952/1‑140 (MQ=255)                                                                                                                   gcagcTTACGCTGTGCAACCAGTGCCTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCt                <  1:159541/140‑1 (MQ=255)                                                                                                                        ttACGCTGTGCAACCAGTGCCTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGcc           <  2:118225/140‑1 (MQ=255)                                                                                                                             cTGTGCAACCAGTGCCTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAaat      <  1:374868/140‑1 (MQ=255)                                                                                                                                tgCAACCAGTGCCTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAaatcaa   >  2:54672/1‑140 (MQ=255)                                                                                                                                tgCAACCAGTGCCTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCACCGTTGCGCCCAGCGCCTTATCGCCTTTGCCCAaatcaa   >  2:287799/1‑140 (MQ=255)                                                                                                                                 gCAACCAGTGCCTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCCGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAaatcaat  <  2:644999/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                    CCAGGCGATCGTCACCCAGAGTGGCGATATCCGCGTCAATGCCACAATGACCAGCAAAGGCCATTTCCGCCAGCGTTACCAGCAGGCCGCCATCAGAGCGGTCGTGATACGCCAGCAGCTTACGCTGTGCAACCAGCGCCTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAAT  >  NC_000913/2692792‑2693059

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑