Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,709,188	T→C	T73A (ACA→GCA)	rseA ←	anti‑sigma factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,709,188	0	T	C	100.0%	96.7 / NA	28	T73A (ACA→GCA)	rseA	anti‑sigma factor
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (11/17);  total (11/17)

GACGCCAACGATAACTGCAAGCGATACGCATGCGGCTACGCCCATTTGGGTAAGCTGTGCCGCCCACGGACGTACTTTCTGCCAGAATGGCATTTTCTGCCATTGATGCGGCGCAGGCTGGGCTTCCGGGATCAATGTCGCCGGTTGACGTACTGGCTCTTCTTCAATGGCGGCCATCACGCGTGAAGAGATATCGAAATGGAGCACCTCGGGAGTATCACCCCGCATTGAGTCACGGATTAAGTGATAGCTTTC  >  NC_000913/2709051‑2709305                                                                                                                                          |                                                                                                                      gACGCCAACGATAACTGCAAGCGATACGCATGCGGCTACGCCCATTTGGGTAAGCTGTGCCGCCCACGGACGTACTTTCTGCCAGAATGGCATTTTCTGCCATTGATGCGGCGCAGGCTGGGCTTCCGGGATCAATGCCg                                                                                                                     >  1:423073/1‑140 (MQ=255)   cGCCAACGATAACTGCAAGCGATACGCATGCGGCTACGCCCATTTGGGTAAGCTGTGCCGCCCACGGACGTACTTTCTGCCAGAATGGCATTTTCTGCCATTGATGCGGCGCAGGCTGGGCTTCCGGGATCAATgccgcc                                                                                                                   <  2:16152/140‑1 (MQ=255)        aCGATAACTGCAAGCGATACGCATGCGGCTACGCCCATTTGGGTAAGCTGTGCCGCCCACGGACGTACTTTCTGCCAGAATGGCATTTTCTGCCATTGATGCGGCGCAGGCTGGGCTTCCGGGATCAATGCCGCCGGTTg                                                                                                              <  2:121207/140‑1 (MQ=255)               cTGCAAGCGATACGCATGCGGCTACGCCCATTTGGGTAAGCTGTGCCGCCCACGGACGTACTTTCTGCCAGAATGGCATTTTCTGCCATTGATGCGGCGCAGGCTGGGCTTCCGGGATCAATGCCGCCGGTTGACGTACt                                                                                                       <  1:137161/140‑1 (MQ=255)                    aGCGATACGCATGCGGCTACGCCCATTTGGGTAAGCTGTGCCGCCCACGGACGTACTTTCTGCCAGAATGGCATTTTCTGCCATTGATGCGGCGCAGGCTGGGCTTCCGGGATCAATGCCGCCGGTTGACGTACTGGctc                                                                                                  <  2:662177/140‑1 (MQ=255)                    aGCGATACGCATGCGGCGACGCCCATTTGGGGAAGGTGTGCCGCCCACGGACGTACTTTCTGCCAGAATGGCATTTTCTGCCATTGATGCGGCGCAGGCTGGGCTTCCGGGATCAATGCCGCCGGTTGACGTACTGGctc                                                                                                  <  2:228567/140‑1 (MQ=255)                     gCGATACGCATGCGGCTACGCCCATTTGGGTAAGCTGTGCCGCCCACGGACGTACTTTCTGCCAGAATGGCATTTTCTGCCATTGATGCGGCGCAGGCTGGGCTTCCGGGATCAATGCCGCCGGTTGACGTACTGGctct                                                                                                 <  1:99028/140‑1 (MQ=255)                                gCGGCTACGCCCATTTGGGTAAGCTGTGCCGCCCACGGACGTACTTTCTGCCAGAATGGCATTTTCTGCCATTGATGCGGCGCAGGCTGGGCTTCCGGGATCAATGCCGCCGGTTGACGTACTGGCTCTTCTTCAATggc                                                                                      >  1:332671/1‑140 (MQ=255)                                 cGGCTACGCCCATTTGGGTAAGCTGTGCCGCCCACGGACGTACTTTCTGCCAGAATGGCATTTTCTGCCATTGATGCGGCGCAGGCTGGGCTTCCGGGATCAATGCCGCCGGTTGACGTACTGGCTCTTCTTCAATggcg                                                                                     >  1:574554/1‑140 (MQ=255)                                      aCGCCCATTTGGGTAAGCTGTGCCGCCCACGGACGTACTTTCTGCCAGAATGGCATTTTCTGCCATTGATGCGGCGCAGGCTGGGCTTCCGGGATCAATGCCGCCGGTTGACGTACTGGCTCTTCTTCAATGGCGGCCAt                                                                                <  1:116542/140‑1 (MQ=255)                                                                 cACGGACGTACTTTCTGCCAGAATGGCATTTTCTGCCATTGATGCGGCGCAGGCTGGGCTTCCGGGATCAATGCCGCCGGTTGACGTACTGGCTCTTCTTCAATGGCGGCCATCACGCGTGAAGAGATATCGAAATGGAg                                                     >  1:48513/1‑140 (MQ=255)                                                                 cACGGACGTACTTTCTGCCAGAATGGCATTTTCTGCCATTGATGCGGCGCAGGCTGGGCTTCCGGGATCAATGCCGCCGGTTGACGTACTGGCTCTTCTTCAATGGCGGCCATCACGCGTGAAGAGATATCGAAATGGAg                                                     <  2:118620/140‑1 (MQ=255)                                                                 cACGGACGTACTTTCCGCCAGACTGCCATCTTCTGCCTTTGATGCGGCGCAGGCTGGGCTTCCGGGATCAATGCCGCCGGTTGACGTACTGGCTCTTCTTCAATGGCGGCCATCACGCGTGAAGAGATATCGAAATGGAg                                                     <  1:208870/140‑1 (MQ=255)                                                                    ggACGTACTTTCTGCCAGAATGGCATTTTCTGCCATTGATGCGGCGCAGGCTGGGCTTCCGGGATCAATGCCGCCGGTTGACGTACTGGCTCTTCTTCAATGGCGGCCATCACGCGTGAAGAGATATCGAAATGGAGCAc                                                  >  1:441370/1‑140 (MQ=255)                                                                      aCGTACTTTCTGCCAGAATGGCATTTTCTGCCATTGATGCGGCGCAGGCTGGGCTTCCGGGATCAATGCCGCCGGTTGACGTACTGGCTCTTCTTCAATGGCGGCCATCACGCGTGAAGAGATATCGAAATGGAGCACCt                                                <  2:449422/140‑1 (MQ=255)                                                                              tCTGCCAGAATGGCATTTTCTGCCATTGATGCGGCTCAGTCTGGGCTTCCGGGATCAATGCCGCCGGTTGACGTACTGGCTCTTCTTCAATGGCGGCCATCACGCGTGAAGAGATATCGAAATGGAGCACCTCGGGAGTa                                        <  2:220825/140‑1 (MQ=255)                                                                              tCTGCCAGAATGGCATTTTCTGCCATTGATGCGGCGCAGGCTGGGCTTCCGGGATCAATGCTGCCGGTTGACGTACTGGCTCTTCTTCAATGGCGGCCATCACGCGTGAAGAGATATCGAAATGGAGCACCTCGGGAGTa                                        <  2:120634/140‑1 (MQ=255)                                                                              tCTGCCAGAATGGCATTTTCTGCCATTGATGCGGCGCAGGCTGGGCTTCCGGGATCAATGCCGCCGGTTGACGTACTGGCTCTTCTTCAATGGCGGCCATCACGCGTGAAGAGATATCGAAATGGAGCACCTCGGGAGTa                                        <  2:441370/140‑1 (MQ=255)                                                                              tCTGCCAGAATGGCATTTTCTGCCATTGATGCGGCGCAGGCTGGGCTTCCGGGATCAATGCCGCCGGTTGACGTACTGGCTCTTCTTCAATGGCGGCCATCACGCGTGAAGAGATATCGAAATGGAGCACCTCGGGAGTa                                        >  2:379260/1‑140 (MQ=255)                                                                              tCTGCCAGAATGGCATTTTCTGCCATTGATGCGGCGCAGGCTGGGCTTCCGGGATCAATGCCGCCGGTTGACGTACTGGCTCTTCTTCAATGGCGGCCATCACGCGTGAAGAGATATCGAAATGGAGCACCTCGGGAGTa                                        <  2:649768/140‑1 (MQ=255)                                                                                tGCCAGAATGGCATTTTCTGCCATTGATGCGGCGCAGGCTGGGCTTCCGGGATCAATGCCGCCGGTTGACGTACTGGCTCTTCTTCAATGGCGGCCATCACGCGTGAAGAGATATCGAAATGGAGCACCTCGGGAGTATc                                      >  2:277332/1‑140 (MQ=255)                                                                                tGCCAGAATGGCATTTTCTGCCATTGATGCGGCGCAGGCTGGGCTTCCGGGATCAATGCCGCCGGTTGACGTACTGGCTCTTCTTCAATGGCGGCCATCACGCGTGAAGAGATATCGAAATGGAGCACCTCGGGAGTATc                                      >  2:343909/1‑140 (MQ=255)                                                                                      aaTGGCATTTTCTGCCATTGATGCGGCGCAGGCTGGGCTTCCGGGATCAATGCCGCCGGTTGACGTACTGGCTCTTCTTCAATGGCGGCCATCACGCGTGAATAGATATCAAAAAGGCGCACCTAGGGAGTATCACCCCg                                >  1:305263/1‑140 (MQ=255)                                                                                              tttCTGCCATTGATGCGGCGCAGGCTGGGCTTCCGGGATCAATGCCGCCGGTTGACGTACTGGCTCTTCTTCAATGGCGGCCATCACGCGTGAAGAGATATCGAAATGGAGCACCTCGGGAGTATCACCCCGCTTTGAGt                        <  2:583133/140‑1 (MQ=255)                                                                                              tttCTGCCATTGATGCGGCGCACGCGGGGCTTCCGGGGTCAATGCCGCCGGTTGACGTACTGGCTCTTCTTCAATGGCGGCCATCACGCGTGAAGAGATATCGAAATGGAGCACCTCGGGAGTATCACCCCGCATTGAGt                        <  1:255776/140‑1 (MQ=255)                                                                                                tCTGCCATTGATGCGGCGCAGGCTGGGCTTCCGGGATCAATGCCGCCGGTTGACGTACTGGCTCTTCTTCAATGGCGGCCATCACGCGTGAAGAGATATCGAAATGGAGCGCCTTGGAAGTATCACCCCGCATTGAGTCa                      >  1:298790/1‑140 (MQ=255)                                                                                                tCTGCCATTGATGCGGCGCAGGCTGGGCTTCCGGGATCAATGCCGCCGGTTGACGTACTGGCTCTTCTTCAATGGCGGCCATCACGCGTGAAGAGATATCGAAATGGAGCACCTCGGGAGTATCACCCCGCATTGAGTCa                      <  2:100341/140‑1 (MQ=255)                                                                                                tCTGCCATTGATGCGGCGCAGGCTGGGCTTCCGGGATCAATGCCGCCGGTTGACGTACTGGCTCTTCTTCAATGGCGGCCATCACGCGTGAAGAGATATCGAAATGGAGCACCTCGGGAGTATCACCCCGCATTGAGTCa                      <  1:537901/140‑1 (MQ=255)                                                                                                                    ggCTGGGCTTCCGGGATCAATGCCGCCGGTTGACGTACTGGCTCTTCTTCAATGGCGGCCATCACGCGTGAAGAGATATCGAAATGGAGCACCTCGGGAGTAACACCCCGCATTGAGGCACGGATTAAGTGATAGCTTTc  >  1:290255/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                      GACGCCAACGATAACTGCAAGCGATACGCATGCGGCTACGCCCATTTGGGTAAGCTGTGCCGCCCACGGACGTACTTTCTGCCAGAATGGCATTTTCTGCCATTGATGCGGCGCAGGCTGGGCTTCCGGGATCAATGTCGCCGGTTGACGTACTGGCTCTTCTTCAATGGCGGCCATCACGCGTGAAGAGATATCGAAATGGAGCACCTCGGGAGTATCACCCCGCATTGAGTCACGGATTAAGTGATAGCTTTC  >  NC_000913/2709051‑2709305

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑