| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,723,976 | A→G | H417R (CAC→CGC) | pssA → | phosphatidylserine synthase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,723,976 | 0 | A | G | 97.5% | 124.9 / NA | 41 | H417R (CAC→CGC) | pssA | phosphatidylserine synthase |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (17/22); minor base C (1/0); total (19/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.50e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TAACCTGAACCCGCGCGCCTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCACAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCACTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGT > NC_000913/2723845‑2724106 | taaCCTGAACCCGCGCTCCTGGTGTCTGGAACTTGGAAAGGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCACAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCGCTATcgcg < 2:320133/140‑1 (MQ=255)taaCCTGAACCCGCGCGCCTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCACAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCGCTATcgcg < 2:529751/140‑1 (MQ=255)taaCCTGAACCCGCGCGCCTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCACAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCGCTATcgcg < 2:378874/140‑1 (MQ=255)taaCCTGAACCCGCGCGCCTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCACAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCGCTATcgcg < 2:84830/140‑1 (MQ=255) tGAACCCGCGCGCCTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCACAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCGCTATCGCGATCTg < 1:410675/140‑1 (MQ=255) aaCCCGCGCGCCTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCACAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCTTCGTTAAGCGCTATCGCGATCTGCa > 1:206202/1‑140 (MQ=255) aaCCCGCGCGCCTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCACAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCGCTATCGCGATCTGCa > 1:142078/1‑140 (MQ=255) aaCCCGCGCGCCTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCACAGCGAGAGAAAGAACTGGAGCTGATCCGCCAGCATACAACCCTCGTTAAGCCCTATCGCGATCTGCa > 1:300160/1‑140 (MQ=255) gcgcgcCTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCACAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCGCTATCGCGATCTGCAAAGTa > 1:656479/1‑140 (MQ=255) gcgcgcCTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCACAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCGCTATCGCGATCTGCAAAGTa > 1:401259/1‑140 (MQ=255) cgcgcCTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCACAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCGCTATCGCGATCTGCAAAGTAt > 2:523141/1‑140 (MQ=255) gcgcCTGGCGTCTGGATGTGGATAACGCCATGTCGGTCCGCGATCCGCAACTTGAGCTGGCGCCACAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCGCTATCGCGATCTGCAAAGTAtt < 2:294822/140‑1 (MQ=255) gcgcCTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCACAGCGAGAGAAAGAACTGGAGCTGATGCGCGAGCAAACCCCCAGCGTTAAGCGCTATGGCGATCTGCAAAGTAtt > 2:181244/1‑140 (MQ=255) gcgcCTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCACAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATAGCACCATCGTTAGGCTCTATCGGGATCTGCAAAGTAtt > 1:199348/1‑140 (MQ=255) gcgcCTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCACAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCCCGATCGTTAAGCGCTATCGCGATCTGCAAAGTAtt > 2:296208/1‑140 (MQ=255) gTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCACAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCGCTATCGCGATCTGCAAAGTATTGCCGATTAt < 2:142078/140‑1 (MQ=255) atctggaAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCACAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCGCTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTg < 1:110230/140‑1 (MQ=255) tggaAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCACAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCGCTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAg < 1:523141/140‑1 (MQ=255) tCCACGATCCGCAACTTGAGCTGGCGCCACAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCGCTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATccgc > 1:161604/1‑140 (MQ=255) aTCCGCAACTTGAGCTGGCGCCACAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCGCTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTg < 2:161604/140‑1 (MQ=255) gAGCTGGCGCCACAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCGCTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCg > 1:630965/1‑140 (MQ=255) gAGCTGGCGCCACAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCGCTATCGCGATCTGCAAAGAATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCg > 1:425462/1‑140 (MQ=255) aGCTGGCGCCACAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCGCTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGc > 2:656241/1‑140 (MQ=255) ggCGCCACAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCGCTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCg < 2:497797/140‑1 (MQ=255) gcgcCACAGCGAGAGCAAGAACTGGTGCTTATCCGCGCGCATACCACCATCGTTAAGCGCTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGa < 1:181244/140‑1 (MQ=255) cgcCACAGCGAGCGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCGCTATCGCGATCTGCAAAGTACTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGAc > 2:362809/1‑140 (MQ=255) cgcCACAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCGCTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCTTAAACTCTTCCACCCTTTGCGCCGTATCCGCATCGAc > 1:238030/1‑140 (MQ=255) gcCACAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCGCTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGAcc > 2:504259/1‑140 (MQ=255) ggagagaAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTTAGCGCTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGattaat < 2:628182/139‑1 (MQ=255) cGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCGCTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGattaat < 2:51238/140‑1 (MQ=255) aagaaCTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCGCTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGc < 1:362809/140‑1 (MQ=255) agaaCTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCGCTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCa < 2:113148/140‑1 (MQ=255) gaaCTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCGCTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCAt < 2:515043/140‑1 (MQ=255) tGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCGCTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTg < 2:630965/140‑1 (MQ=255) tGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCGCTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCAGTATCCGCATCGACCGATTAATTAGCCGCATCCTg < 2:425462/140‑1 (MQ=255) ggAGCTGATCCGCGAGCATACCACCATCGTTAAGCGCTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGt < 1:656241/140‑1 (MQ=255) tGATCCGCGAGCATACCTCCATGGTTAAGCGCTATGGCGATCTGCAAAATATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATca < 2:238030/140‑1 (MQ=255) cgcgAGCATACCACCATAGTTATGCGCTATCGCGACCTGTAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAAcc < 2:267070/140‑1 (MQ=255) cgcgAGCATACCACCACGGTTATGCGCTATCCCGATCTGGAAAGTATTCCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAAcc < 2:300160/140‑1 (MQ=255) cgAGCATACCACCATCGTTAAGCGCTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAAcccc > 1:488694/1‑140 (MQ=255) aTCGTTAAGCGCTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACgggg > 2:485869/1‑140 (MQ=255) tCGTTAAGCGCTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGgt < 2:92016/140‑1 (MQ=255) tCGTTAAGCGCTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGAACGATTAATTAGCGGGATTCTGTAATAACAACCCCGTCCTGTACGGGgt > 2:317837/1‑140 (MQ=255) | TAACCTGAACCCGCGCGCCTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCACAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCACTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGT > NC_000913/2723845‑2724106 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |