Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,726,164	A→G	noncoding (25/120 nt)	rrfG ←	5S ribosomal RNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,726,164	0	A	G	100.0%	16.8 / NA	7	noncoding (25/120 nt)	rrfG	5S ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (1/6);  total (1/6)

CATCCGTCAGGATGGCCTTTCGCATAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACGGCCGCCAGGCAAATTCTGT  >  NC_000913/2726038‑2726196                                                                                                                               |                                 cATCCGTCAGGATGGCCTTTCGCATAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAATTCCGCATGGGGACAGGAGGGGCCACCGCGCTACg                     >  2:261887/1‑140 (MQ=17)       tCAGGATGGCCTTTCGCATAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGGCCACCGCGCTACGgccgcc               <  1:370917/140‑1 (MQ=255)               gCCTTTCGCATAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGGTCACCGCGCTACGGCCGCCAGGCAAAt       <  1:36366/140‑1 (MQ=255)               gCCTTTCGCATAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGGCCACCGCGCTACGGCCGCCAGGCAAAt       <  1:124815/140‑1 (MQ=38)               gCCTTTCGCATAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGGCCACCGCGCTACGGCCGCCAGGCAAAt       <  1:375617/140‑1 (MQ=38)               gCCTTTCGCATAATTTGATGCCGGGCAGTTCCCTACTCTGGCATGGGGGGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGGCCACCGCGCTACGGCCGCCAGGCAAAt       <  2:288312/140‑1 (MQ=255)                    tCGCATAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGGCCACCGCGCTACGGCCGCCAGGCAAATTCTGt  <  2:467839/140‑1 (MQ=18)                                                                                                                               |                                 CATCCGTCAGGATGGCCTTTCGCATAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACGGCCGCCAGGCAAATTCTGT  >  NC_000913/2726038‑2726196

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑