| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,749,819 | T→C | V349A (GTG→GCG) | yfjD → | putative inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,749,819 | 0 | T | C | 95.1% | 116.8 / ‑2.3 | 41 | V349A (GTG→GCG) | yfjD | putative inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base T (1/1); new base C (20/19); total (21/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.85e-01 | |||||||||||
TGGTCGTCAACGAGTATGGAGACATTCAGGGGCTGGTGACGGTTGAAGATATTCTGGAAGAGATTGTCGGCGATTTCACTACGTCGATGTCGCCAACACTTGCCGAAGAGGTCACGCCGCAAAACGACGGTTCGGTGATTATCGATGGCACCGCCAACGTGCGGGAAATCAACAAAGCCTTTAACTGGCATCTACCGGAAGATGATGCCCGCACGGTTAATGGCGTCATTCTTGAGGCACTGGAAGAGATCCCTGTCGCAGGCACCCGCGT > NC_000913/2749684‑2749954 | tGGTCGTCAACGAGTACAGAGATATTCAGGGGCTGGTGACGGTTGAAGTTATTCTGGAAGAGATTGTCGGCGATTTCACTACGTCGATGTCGCCAACACTTGCCGAAGAGGTCACGCCGCAAAACGACGGTTCGGCGAtt < 2:291638/140‑1 (MQ=255) aaCGAGTATGGAGACATTCAGGGGCTGGTGACGGTTGAAGATATTCTGGAAGAGATTGTCGGCGATTTCACTACGTCGATGTCGCCAACACTTGCCGAAGAGGTCACGCCGCAAAACGACGGTTCGGCGATTATCGATgg > 1:4667/1‑140 (MQ=255) tGGAGACATTCAGGGGCTGGTGACGGTTGAAGATATTCTGGAAGAGATTGTCGGCGATTTCACTACGTCGATGTCGCCAACCCTTGCCGAAGAGGTCACGCCGCAAAACGACGGTTCGGCGATTATCGATgg < 1:582464/132‑1 (MQ=255) tGGAGACATTCAGGGGCTGGTGACGGTTGAAGATATTCTGGAAGAGATTGTCGGCGATTTCACTACGTCGATGTCGCCAACACTTGCCGAAGAGGTCACGCCGCAAAACGACGGTTCGGCGATTATCGATgg > 2:582464/1‑132 (MQ=255) tGGAGACATTCAGGGGCTGGTGACGGTTGAAGATATTCTGGAAGAGATTGTCGGCGATTTCACTACGTCGATGTCGCCAACACTTGCCGAAGAGGTCACGCCGCAAAACGACGGTTCGGCGATTATCGATGGCACCGCCa > 1:597704/1‑140 (MQ=255) aTTCAGGGGCTGGTGACGGTTGAAGATATTCTGGAAGAGATTGTCGGCGATTTCACTACGTCGATGTCGCCAACACTTGCCGAAGAGGTCACGCCGCAAAACGACGGTTCGGCGATTATCGATGGCACCGCCAACGTGCg > 2:621437/1‑140 (MQ=255) aTTCAGGGGCTGGTGACGGTTGAAGATATTCTGGAAGAGATTGTCGGCGATTTCACTACGTCGATGTCGCCAACACTTGCCGAAGAGGTCACGCCGCAAAACGACGGTTCGGCGATTATCGATGGCACCGCCAACGTGCg > 1:455520/1‑140 (MQ=255) ttCAGGGGCTGGTGACGGTTGAAGATATTCTGGAAGAGATTGTCGGCGATTTCACTACGTCGATGTCGCCAACACTTGCCGAAGAGGTCACGCCGCAAAACGACGGTTCGGCGATTATCGATGGCACCGCCAACGTGCgg > 2:4770/1‑140 (MQ=255) ttCAGGGGCTGGTGACGGTTGAAGATATTCTGGAAGAGATTGTCGGCGATTTCACTACGTCGATGTCGCCAACACTTGCCGAAGAGGTCACGCCGCAAAACGACGGTTCGGCGATTATCGATGGCACCGCCAACGTGCgg > 1:593237/1‑140 (MQ=255) ggggCTGGTGACGGTTGAAGATATTCTGGAAGAGATTGTCGGCGATTTCACTACGTCGATGTCGCCAACACTTGCCGAAGAGGTCACGCCGCAAAACGACGGTTCGGCGATTATCGATGGCACCGCCAACGTGCGGGaaa < 1:35919/140‑1 (MQ=255) gCTGGTGACGGTTGAAGATATTCTGGAAGAGATTGTCGGCGATTTCACTACGTCGATGTCGCCAACACTTGCCGAAGAGGTCACGCCGCAAAACGACGGTTCGGCGATTATCGATGGCACCGCCAACGTGCGGGAAATca > 1:605191/1‑140 (MQ=255) cTGGTGACGGTTGAAGATATTCTGGAAGAGATTGTCGGCGATTTCACTACGTCGATGTCGCCAACACTTGCCGAAGAGGTCACGCCGCAAAACGACGGTTCGGCGATTATCGATGGCACCGCCAACGTGCGGGAAATcaa > 1:129512/1‑140 (MQ=255) ggTGACGGTTGAAGATATTCTGGAAGAGATTTTCGTCGATTTCACTACGTCGATGTCGCCAACACTTGCCGAAGAGGTCACGCCGCAAAACGACGGTTCGGCGATTATCGATGGCACCGCCAACGTGCGGGAAATcaaca < 2:231933/140‑1 (MQ=255) gTGACGGTTGAAGATATCCTGGCAGAGATTGTCGGAGATCTCACTACGACGATGTCGCCAACACTTGCCGAAGAGGTCACGCCGCAAAACGACGGTTCGGCGATTATCGATGGCACCGCCAACGTGCGGGAAATcaacaa < 2:172347/140‑1 (MQ=255) atTCTGGAAGAGATTGTCGGCGATTTCACTACGTCGATGTCGCCAACACTTGCCGAAGAGGTCACGCCGCAAAACGACGGTTCGGCGATTATCGATGGCACCGCCAACGTGCGGGAAATCAACAAAGCCTTTAACTGGCa < 1:480765/140‑1 (MQ=255) cTGGAAGAGATTGTCGGCGATTTCACTACGTCGATGTCGCCAACACTTGCCGAAGAGGTCACGCCGCAAAACGACGGTTCGGCGATTATCGATGGCACCGCCAACGTGCGGGAAATCAACAAAGCCTTTAACTGGCATCt < 1:522630/140‑1 (MQ=255) gAAGAGATTGTCGGCGATTTCACTACGTCGATGTCGCCAACACTTGCCGAAGAGGTCACGCCGCAAAACGACGGTTCGGCGATTATCGATGGCACCGCCAACGTGCGGGAAATCAACAAAGCCTTTAACTGGCATCTAcc > 2:499119/1‑140 (MQ=255) aaGAGATTGTCGGCGATTTCACTACGTCGATGTCGCCAACACTTGCCGAAGAGGTCACGCCGCAAAACGACGGTTCGGCGATTATCGATGGGACCGCCAAGGTGAGGGGAATCAACAGAGCCTTTAACTGGCATCTACCg > 2:181025/1‑140 (MQ=255) agagATTGTCGGCGATTTCACTACGTCGATGTCGCCAACACTTGCCGAAGAGGTCACGCCGCAAAACGACGGTTCGGTGATTATCGATGGCACCGCCAACGTGCGGGAAATCAACAAAGCCTTTAACTGGCATCTACCgg > 2:489613/1‑140 (MQ=255) aTTGTCGGCGATTTCACTACGTCGATGTCGCCAACACTTGCCGAAGAGGTC‑CGCCGCAAAACGACGGTTCGGCGATTATCGATGGCACCGCCAACGTGCGGGAAATCAACAAAGCCTTTAACTGGCATCTACCGGAAgat > 1:452071/1‑140 (MQ=255) tttCACTACGTCGATGTCGCCAACACTTGCCGAAGAGGTCACGCCGCAAAACGACGGTTCGGCGATTATCGATGGCACCGCCAACGTGCGGGAAATCAACAAAGCCTTTAACTGGCATCTACCGGAAGATGATGCCCGCa < 1:4770/140‑1 (MQ=255) ttCACTACGTCGATGTCGCCAACACTTGCCGAAGAGGTCACGCCGCAAAACGACGGTTCGGCGATTATCGATGGCACCGCCAACGTGCGGGAAATCAACAAAGCCTTTAACTGGCATCTACCGGAAGATGATGCCCGCAc < 1:638540/140‑1 (MQ=255) gATGTCGCCAACACTTGCCGAAGAGGTCACGCCGCAAAACGACGGTTCGGCGATTATCGATGGCACCGCCAACGTGCGGGAAATCAACAAAGCCTTTAACTGGCATCTACCGGAAGATGATGCCCGCACGGTTAATGGCg < 2:597704/140‑1 (MQ=255) tGTCGCCAACACTTGCCGAAGAGGTCACGCCGCAAAACGACGGTTCGGCGATTATCGATGGCACCGCCAACGTGCGGGAAATCAACAAAGCTTTTAACTGGCATATACAGGAAGATGGTGCCCGCACGGTTAATGGCGTc > 2:244301/1‑140 (MQ=255) tGTCGCCAACACTTGCCGAAGAGGTCACGCCGCAAAACGACGGTTCGGCGATTATCGATGGCACCGCCAACGTGCGGGAAATCAACAAAGCCTTTAACTGGCATCTACCGGAAGATGATGCCCGCACGGTTAATGGCGTc > 2:166967/1‑140 (MQ=255) gTCGCCAACACTTGCCGAAGAGGTCACGCCGCAAAACGACGGTTCGGCGATTATCGATGGCACCGCCAACGTGCGGGAAATCAACAAAGCCTTTAACTGGCATCTACCGGAAGATGATGCCCGCACGGTTAATGGCGTCa < 1:450732/140‑1 (MQ=255) aaCACTTGCCGAAGAGGTCACGCCGCAAAACGACGGTTCGGCGATTATCGATGGCACCGCCAACGTGCGGGAAATCAACAAAGCCTTTAACCGGCATCTAGCGGAAGAAGATGCCCGCACGGGTAATGGCGTCATTCTTg > 2:240476/1‑140 (MQ=255) acacTTGCCGAAGAGGTCACGCCGCAAAACGACGGTTCGGCGATTATCGATGGCACCGCCAACGTGCGGGAAATCAACAAAGCCTTTAACTGGCATCTACCGGAAGATGATGCCCGCACGGTTAATGGCGTCATTCTTGa < 1:344563/140‑1 (MQ=255) cacTTGCCGAAGAGGTCACGCCGCAAAACGACGGTTCGGCGATTATCGATGGCACCGCCAACGTGCGGGAAATCAACAAAGCCTTTAACTGGCATCTACCGGAAGATGATGCCCGCACGGTTAATGGCGTCATTCTTGAg > 1:468960/1‑140 (MQ=255) acTTGCCGAAGAGGTCACGCCGTAACACGCCGGTTCGACGATTATCGATGGCACCGCCAACGTGCGGGAAATCAACAAAGCCTTTAACTGGCATCTACCGGAAGATGATGCCCGCACGGTTAATGGCGTCATTCTTGAgg < 1:244353/140‑1 (MQ=255) acTTGCCGAAGAGGTCACGCCGCAAAACGACGGTTCGGCGATTATCGATGGCACCGCCAACGTGCGGGAAATCAACAAAGCCTTTAACTGGCATCTACCGGAAGATGATGCCCGCACGGTTAATGGCGTCATTCTTGAgg < 2:605191/140‑1 (MQ=255) acTTGCCGAAGAGGTCACGCCGCAAAACGACGGTTCGGCGATTATCGATGGCACCGCCAACGTGCGGGAAATCAACAAAGCCTTTAACTGGCATCTACCGGAAGATGATGCCCGCACGGTTAATGGCGTCATTCTTGAgg < 1:166967/140‑1 (MQ=255) tGCCGAAGAGGTCACGCCGCAAAACGACGGTTCGGTGATTATCGATGGCACCGCCAACGTGCGGGAAATCAACAAAGCCTTTAACTGGCATCTACCGGAAGATGATGCCCGCACGGTTAATGGCGTCATTCTTGAGGCAc < 1:489613/140‑1 (MQ=255) gCCGAAGAGGTCACGCCGCAAAACGACGGTTCGGCGATTATCGATGGCACCGCCAACGTGCGGGAAATCAACAAAGCCTTTAACTGGCCTCTACCGGAAGATGATGCCCGCACGGTTAATGGCGTCATTCTTGAGGCACt > 2:104114/1‑140 (MQ=255) gCCGAAGAGGTCACGCCGCAAAACGACGGTTCGGCGATTATCGATGGCACCGCCAACGTGCGGGAAATCAACAAAGCCTTTAACTGGCATCTACCGGAAGATGAGGCCGGCACGGTTTATGGTGTCATTCTTGAGGCACt > 2:229346/1‑140 (MQ=255) gagGTCACGCCGCAAAACGACGGTTCGGCGATTATCGATGGCACCGCCAACGTGCGGGAAATCAACAAAGCCTTTAACTGGCATCTACCGGAAGATGATGCCCGCACGGTTAATGGCGTCATTCTTGAGGCACTGGAaga > 2:72192/1‑140 (MQ=255) gccgcAAAACGACGGATCGGCGTTTCTCGCTGGCACCTCCAACGTGCGGGAAATCAACAAAGCCTTTAACTGGCATCTACCGGAAGATGATGCCCGCACGGTTAATGGCGTCATTCTTGAGGCACTGGAAGAGATCCCTg < 1:181025/140‑1 (MQ=255) cgcAAAACGACGGTTCGGCGATTATCGATGGCACCGCCAACGTGCGGGAAATCAACAAAGCCTTTAACTGGCATCTACCGGAAGATGATGCCCGCACGGTTAATGGCGTCATTCTTGAGGCACTGGAAGAGATCCCTGTc < 2:129512/140‑1 (MQ=255) cAAAACGACGGTTCGGCGATTATCGATGGCACCGCCAACGTGCGGGAAATCAACAAAGCCTTTAACTGGCATCTACCGGAAGATGATGCCCGCACGGTTAATGGCGTCATTCTTGAGGCACTGGAAGAGATCCCTGTCGc < 2:452071/140‑1 (MQ=255) aaaaCGACGGTTCGGCGATTATCGATGGCACCGCCAACGTGCGGGAAATCAACAAAGCCTTTAACTGGCATCTACCGGAAGATGATGCCCGCACGGTTAATGGCGTCATTCTTGAGGCACTGGATGAGATCCCTGTCGCa > 1:319162/1‑140 (MQ=255) tCGGCGATTATCGATGGCACCGCCAACGTGCGGGAAATCAACAAAGCCTTTAACTGGCATCTACCGGAAGATGATGCCCGCACGGTTAATGGCGTCATTCTTGAGGCACTGGAAGAGATCCCTGTCGCAGGCACCCgcgt < 2:4667/140‑1 (MQ=255) tCGGCGATTATCGATGGCACCGCCAACGTGCGGGAAATCAACAAAGCCTTTAACTGGCATCTACCGGAAGATGATGCCCGCACGGTTAATGGCGTCATTCTTGAGGCACTGGAAGAGATCCCTGTCGCAGGCACCCgcgt < 1:499119/140‑1 (MQ=255) | TGGTCGTCAACGAGTATGGAGACATTCAGGGGCTGGTGACGGTTGAAGATATTCTGGAAGAGATTGTCGGCGATTTCACTACGTCGATGTCGCCAACACTTGCCGAAGAGGTCACGCCGCAAAACGACGGTTCGGTGATTATCGATGGCACCGCCAACGTGCGGGAAATCAACAAAGCCTTTAACTGGCATCTACCGGAAGATGATGCCCGCACGGTTAATGGCGTCATTCTTGAGGCACTGGAAGAGATCCCTGTCGCAGGCACCCGCGT > NC_000913/2749684‑2749954 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |