Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,777,401	T→C	V96A (GTA→GCA)	yfjZ →	CP4‑57 prophage; putative antitoxin of the YpjF‑YfjZ toxin‑antitoxin system

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,777,401	0	T	C	100.0%	95.8 / NA	28	V96A (GTA→GCA)	yfjZ	CP4‑57 prophage; putative antitoxin of the YpjF‑YfjZ toxin‑antitoxin system
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (21/7);  total (21/7)

GTATTTCCACATTTTATCAGCCAGATAGAGTCGATGCTGACCACTGGTGAACTGAATCCCCGCCATGCCCAATGCGTCACCCTGTACCACAACGGTTTTACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGTATACATCGCTGTTTACCCCACTCAACGCTAACTAATTTCACGAGAGCAAGCATGAACACTCTACCTGCTACAATTTCGCAGGCGGCGAAGCCCTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTACTGACAC  >  NC_000913/2777265‑2777534                                                                                                                                         |                                                                                                                                      gTATTTCCACATTTTATCAGCCCGACAGAGTCGATGCTGACCACTGGTGAACTGAATCCCCGCCATGCCCAATGCGTCACCCTGTACCACAACGGTTTTACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGcata                                                                                                                                    <  1:261867/140‑1 (MQ=255) gTATTTCCACATTTTATCAGCCAGATAGAGTCGATGCTGACCACTGGTGAACTGAATCCCCGCCATGCCCAATGCGTCACCCTGTACCACAACGGTTTTACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGcata                                                                                                                                    <  2:153774/140‑1 (MQ=255)  tATTTCCACATTTTATCAGCCAGATAGAGTCGATGCTGACCACTGGTGAACTGAATCCCCGCCATGCCTAATGCGTCACCCTGTACCACAACGGTTTTACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGCATAc                                                                                                                                   >  2:396484/1‑140 (MQ=255)      tCCACATTTTATCAGCCAGATAGAGTCGATGCTGACCACTGGTGAACTGAATCCCCGCCATGCCCAATGCGTCACCCTGTACCACAACGGTTTTACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGCATACATCg                                                                                                                               <  1:551632/140‑1 (MQ=255)            ttttATCAGCCAGATAGAGTCGATGCTGACCACTGGTGAACTGAATCCCCGCCATGCCCAATGCGTCACCCTGTACCACAACGGTTTTACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGCATACATCGCTGttt                                                                                                                         >  2:520493/1‑140 (MQ=255)                         aTAGAGTCGATGCTGACCACTGGTGAACTGAATCCCCGCCATGCCCAATGCGTCACCCTGTACCACAACGGTTTTACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGCATACATCGCTGTTTACCCCACTCAACg                                                                                                            <  2:146837/140‑1 (MQ=255)                               tCGATGCTGACCACTGGTGAACTGAATCCCCGCCATGCCCAATGCGTCACCCTGTACCACAACGGTTTTACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGCATACATCGCTGTTTAc                                                                                                                       >  1:133398/1‑123 (MQ=255)                               tCGATGCTGACCACTGGTGAACTGAATCCCCGCCATGCCCAATGCGTCACCCTGTACCACAACGGTTTTACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGCATACATCGCTGTTTAc                                                                                                                       <  2:133398/123‑1 (MQ=255)                                 gATGCTGACCACTGGTGAACTGAATCCCCGCCATGCCCAATGCGTCACCCTGTACCACAACGGTTTTACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGCATACAGCGCAGTTTACCCCACTCAACGctaactaa                                                                                                    >  1:266661/1‑140 (MQ=255)                                  aTGCTGACCACTGGTGAACTGAATCCCCGCCATGCCCAATGCGTCACCCTGTACCACAACGGTTTTACCTGCGAAGCCGATACTCTTGGTATTTGCGGCTACGCGTACATCGCTGTTGACCCCCCTCAACGATAACTAAt                                                                                                   >  2:313426/1‑140 (MQ=255)                                             tGGTGAACTGAATCCCCGCCATGCCCAATGCGTCACCCTGTACCACAACGGTTTTACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGCATACATCGCTGTTTACCCCACTCAACGCTAACTAATTTCACGAGagc                                                                                        >  2:116382/1‑140 (MQ=255)                                               gTGAACTGAATCCCCGCCATGCCCAATGCGTCACCCTGTACCACAACGGTTTTACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGCATACATCGCTGTTTACCCCACTCAACGCTAACTAATTTCACGAGagcaa                                                                                      >  1:558309/1‑140 (MQ=255)                                                taacctaaaTCCCCGCCATGCCCAATGCGTCACCCTGTACCACAACGGTTTTACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGCATACATCGCTGTTTACCCCACTCAACGCTAACTAATTTCACGAGagcaag                                                                                     <  2:558309/133‑1 (MQ=255)                                                  aaCTGAATCCCCGCCATGCCCAATGCGTCACCCTGTACCACAACGGTTTTACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGCATAGATCGCTGTTTACCCCACTCAACGCTTACTAATTTCACGAGagcaagca                                                                                   >  2:238783/1‑140 (MQ=255)                                                       aaTCCCCGCCATGCCCAATGCGTCACCCTGTACCACAACGGTTTTACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGCATACATCGCTGTTTACCCCCCTCAACGCTAACTAATTTCACCAGAGCAAGCATGAac                                                                              >  1:262397/1‑140 (MQ=255)                                                       aaTCCCCGCCATGCCCAATGCGTCACCCTGTACCACAACGGTTTTACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGCATACATCGCTGTTTACCCCACTCAACGCTAACTAATTTCACGAGAGCAAGCATGAac                                                                              >  1:412395/1‑140 (MQ=255)                                                            ccGCCATGCCCAATGCGCCACCCTGTACCACCACGGTTTTACCTGCGACGCCGATACTCTTGGTAGTTGCGGCTACGCATACATCGCTGTTTACCCCACTCAACGCTAACTAATTTCACGAGAGCAAGCATGAACActct                                                                         <  2:213093/140‑1 (MQ=255)                                                               ccATGCCCAATGCGTCACCCTGTACCACAACGGTTTTACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGCATACATCGCTGTTTACCCCACTCAACGCTAACTAATTTCACGAGAGCAAGCATGAACACTCTAcc                                                                      <  2:412395/140‑1 (MQ=255)                                                                     ccAATGCGTCACCCTGTACCACAACGGTTTTACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGCATACATCGCTGTTTACCCCACTCAACGCTAACTAATTTCACGAGAGCAAGCATGAACACTCTACCTGCTAc                                                                >  1:556565/1‑140 (MQ=255)                                                                              cACCCTGTACCACAACGGTTTTACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGCATACATCGCTGTTTACCCCACTCAACGCTAACTAATTTCACGAGAGCAAGCATGAACACTCTACCTGCTACAATTTCGCa                                                       >  2:525641/1‑140 (MQ=255)                                                                                    gTACCACAACGGTTTTACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGCATACATCGCTGTTTACCCCACTCAACGCTAACTAATTTCACGAGAGCAAGCATGAACACTCTACCTGCTACAATTTCGCAggcggc                                                 <  1:520493/140‑1 (MQ=255)                                                                                     tACCACAACGGTTTTACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGCATACATCGCTGTTTACCCCACTCAACGCTAACTAATTTCACGAGAGCAAGCATGAACACTCTACCTGCTACAATTTCGCAGgcggcg                                                >  1:437676/1‑140 (MQ=255)                                                                                     tACCACAACGGTTTTACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGCATACATCGCTGTTTACCCCACTCAACGCTAACTAATTTCACGAGAGCAAGCATGAACACTCTACCTGCTACAATTTCGCAGgcggcg                                                >  2:329442/1‑140 (MQ=255)                                                                                          caACGGTTTTACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGCATACATCGCTGTTTACCCCACTCAACGCTAACTAATTTCACGAGAGCAAGCATGGACACTCTCCCTGCTACAATTTGGCAGGCGGCGAAGcc                                           >  1:181098/1‑140 (MQ=255)                                                                                          caACGGTTTTACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGCATACATCGCTGTTTACCCCACTCAACGCTAACTAATTTCACGAGAGCAAGCATGAACACTCTACCTGCTACAATTTCGCAGGCGGCGAAGcc                                           >  2:78345/1‑140 (MQ=255)                                                                                                         cGAAGCCGATACTCTTGGTAGTTGCGGCTACGCATACATCGCTGTTTACCCCACTCAACGCTAACTAATTTCACGAGAGCAAGCATGAACACTCTACCTGCTACAATTTCGCAGGCGGCGAAGCCCTGCCTGTCGCCAGt                            >  1:40496/1‑140 (MQ=255)                                                                                                                    ctctTGGTAGTTGCGTCTACGCGTAGATCCCTGTTTACCCCACTCAACGCTAACTAATTTCACGAGAGCAAGCATGAACACTCTACCTGCTACAATTTCGCAGGCGGCGAAGCCCTGCCTGTCGCCAGTGGCTGTCTGGc                 <  1:313426/140‑1 (MQ=255)                                                                                                                            aGTTGCGGCTACGCATACATCGCTGTTTACCCCACTCAACGCTAACTAATTTCACGAGAGCAAGCATGAACACTCTACCTGCTACAATTTCGCAGGCGGCGAAGCCCTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTa         >  1:384387/1‑140 (MQ=255)                                                                                                                              ttGCGGCTACGCATACATCGCTGTTTACCCCACTCAACGCTAACTAATTTCACGAGAGCAAGCATGAACACTCTACCTGCTACAATTTCGCAGGCGGCGAAGCCCTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTACt       >  1:518502/1‑140 (MQ=255)                                                                                                                                 cGGCTACGCATACATCGCTGTTTACCCCACTCAACGCTAACTAATTTCACGAGAGCAAGCATGAACACTCTACCTGCTACAATTTCGCAGGCGGCGAAGCCCTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTACTGac    >  1:625877/1‑140 (MQ=255)                                                                                                                                   gCTACGCATACATCGCTGTTTACCCCACTCAACGCTAACTAATTTCACGAGAGCAAGCATGAACACTCTACCTGCTACAATTTCGCAGGCGGCGAAGCCCTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTACTGacac  >  2:474942/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                      GTATTTCCACATTTTATCAGCCAGATAGAGTCGATGCTGACCACTGGTGAACTGAATCCCCGCCATGCCCAATGCGTCACCCTGTACCACAACGGTTTTACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGTATACATCGCTGTTTACCCCACTCAACGCTAACTAATTTCACGAGAGCAAGCATGAACACTCTACCTGCTACAATTTCGCAGGCGGCGAAGCCCTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTACTGACAC  >  NC_000913/2777265‑2777534

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑