Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,779,192	T→C	T1179A (ACC→GCC)	ypjA ←	adhesin‑like autotransporter YpjA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,779,192	0	T	C	100.0%	65.3 / NA	20	T1179A (ACC→GCC)	ypjA	adhesin‑like autotransporter YpjA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (13/7);  total (13/7)

AATGCGTTTCTCCGGAACGGGTGTCGGCGTTGGATCGGGAGTAGGGTCTGGTTTTGGATTTGGGTCTGGCTTTGGATCGGGTTTTGGGTCTGGCTTTGGATTTGGGATGGGGTCCGGGTTGGGTTTGACATCATTGGTCAGGTTCCAGTTGCTGTTGCCGTCACTTTTCAGGACATACTCATAGGTCCCAAGATCAACGAAACCGCCGGTATTGCCCAGCGTAAACGAAGCATCCCCTCCCCCTGTTTTCACCA  >  NC_000913/2779055‑2779308                                                                                                                                          |                                                                                                                     aaTGCGTTTCTCCGGAACGGGTGTCGGCGTTGGATC‑GGAGTAGGGTCTGGTTTTGGATTTGGGTCTGGCTTTGGATCGGGTTTTGGGTCTGGCTTTGGATTTGGGATGGGGTCCGGGTTGGGTTTGACATCATTGGCCAg                                                                                                                   >  1:369654/1‑140 (MQ=255)                    ggTGTCGGCGTTGGATCGGGAGTAGGGTCTGGTTTTGGATTTGGGTCTGGCTTTGGATCGGGTTTTGGGTCTGGCTTTGGATTTGGGATGGGGTCCGGGTTGGGTTTGACATCATTGGCCAGGTTCCAGTTGCTGTTGcc                                                                                                 >  1:443693/1‑140 (MQ=255)                      ggtCGGCGTTGGATCGGGAGTAGGGTCTGGTTTTGGATTTGGGTCTGGCTTTGGATCGGGTTTTGGGTCTGGCTTTGGATTTGGGATGGGGTCCGGGTTGGGTTTGACATCATTGGCCAGGTTCCAGTTGCTGTTGCCGt                                                                                               <  2:511313/139‑1 (MQ=255)                             gTTGGATCGGGAGTAGGGTCTGGTTTTGGATTTGGGTCTGGCTTTGGATCGGGTTTTGGGTCTGGCTTTGGATTTGGGATGGGGTCCGGGTTGGGTTTGACATCATTGGCCAGGTTCCAGTTGCTGTTGCCGTCACtttt                                                                                        <  2:49098/140‑1 (MQ=255)                                   tCGGGAGTAGGGTCTTGTTTTGGATCTGGATCTGGCTGTGGATCGGGTTTTGGGTCTGGCTTTGGATTTGGGATGGGGTCCGGGTTGGGTTTGACATCATTGGCCAGGTTCCAGTTGCTGTTGCCGTCACTTTTCAGGAc                                                                                  <  1:259960/140‑1 (MQ=255)                                          tAGGGTCTGGTTTTGGATTTGGGTCTGGCTTTGGATCGGGTTTTGGGTCTGGCTTTGGATTTGGGATGGGGTCCGGGTTGGGTTTGACATCATTGGCCAGGTTCCAGTTGCTGTTGCCGTCACTTTTCAGGACATACTCa                                                                           <  1:72400/140‑1 (MQ=255)                                                 tGGTTTTGGATTTGGGTCTGGCTTTGGATCGGGTTTTGGGTCTGGCTTTGGATTTGGGATGGGGTCCGGGTTGGGTTTGACATCATTGGCCAGGTTCCAGTTGCTGTTGCCGTCACTTTTCAGGACATACTCATAGGTcc                                                                    <  2:369654/140‑1 (MQ=255)                                                 tGGTTTTGGATTTGGGTCTGGCTTTGGATCGGGTTTTGGGTCTGGCTTTGGATTTGGGATGGGGTCCGGGTTGGGTTTGACATCATTGGCCAGGTTCCAGTTGCTGTTGCCGTCACTTTTCAGGACATACTCATAGGTcc                                                                    <  1:93549/140‑1 (MQ=255)                                                  ggTTTTGGATTTGGGTCTGGCTTTGGATCGGGTTTTGGGTCTGGCTTTGGATTTGGGATGGGGTCCGGGTTGGGTTTGACATCATTGGCCAGGTTCCAGTTGCTGTTGCCGTCACTTTTCAGGACATACTCATAGGTccc                                                                   >  1:618988/1‑140 (MQ=255)                                                    ttttGGATTTGGGTCTGGCTTTGGATCGGGTTTTGGGTCTGGCTTTGGATTTGGGATGGGGTCCGGGTTGGGTTTGACATCATTGGCCAGGTTCCAGTTGCTGTTGCCGTCACTATTTAGGACAGACTCATAGGTCCCaa                                                                 >  1:278548/1‑140 (MQ=255)                                                                   tGGCTTTGGATCGGGTTTTGGGTCTGGCTTTGGATTTGGGATGGGGTCCGGGTTGGGTTTGACATCATTGGCCAGGTTCCAGTTGCTGTTGCCGTCACTTTTCAGGACATACTCATAGGTCCCAAGATCAACGAAAccgc                                                  >  2:109669/1‑140 (MQ=255)                                                                      cTTTGGATCGGGTTTTGGGTCTGGCTTTGGATTTGGGATGGGGTCCGGGTTGGGTTTGACATCATTGGCCAGGTTCCAGTTGCTGTTGCCGTCACTTTTCAGGACATACTCATAGGTCCCAAGATCAACGAAACCGCCgg                                               >  1:502175/1‑140 (MQ=255)                                                                         tGGATCGGGTTTTGGGTCTGGCTTTGGATTTGGGATGGGGTCCGGGTTGGGTTTGACATCATTGGCCAGGTTCCAGTTGCTGTTGCCGTCACTTTTCAGGACATACTCATAGGTCCCAAGATCAACGAAACCGCCGGTAt                                            >  1:648695/1‑140 (MQ=255)                                                                            ttggggTTTTGGGTTTGGTTTTGGTTTTGGGAGGGGGTCGGGGTGGGGTTTGACATCATGGGCAAGGTTCCAGTTGCTGTTCCCGTCTTTTTCCAGGCCATCCTCATGGGCCCAAAGATCAACGAAACCGCCGGTATTGc                                         <  2:443693/137‑1 (MQ=255)                                                                            ttCGGGTTTTGGTTCTGGTTTTGGTTTGGGGATGGGGTCCGGGTGGGGTTAGACATCATTGGCCAGGTTCCAGTTGCTGTTGCCGTCACTTTTCAGGACATACTCATAGGTCCCAAGATCAACAAAACCGCCGGTATTGc                                         <  2:399758/139‑1 (MQ=255)                                                                             cacggTTTTGGGTCTGGTTTTGGATTTGGGATGGGGTCCGGGTTGGGTTTGACATCATTGGCCAGGTTCCAGTTGCTGTTGCCGTCACTTTTCAGGACATACTCATAGGTCCCAAGATCAACGAAACCGCCGGTATTGcc                                        <  1:444414/137‑1 (MQ=255)                                                                                           tGGCTTTGGATTTGGGATGGGGTCCGGGTTGGGTTTGACATCATTGGCCAGGTTCCAGTTGCTGTTGCCGTCACTTTTCAGGACATACTCATAGGTCCCAAGATCAACGAAACCGCCGGTATTGCCCAGCGTAAACGAAg                          >  1:27244/1‑140 (MQ=255)                                                                                           tGGCTTTGGATTTGGGATGGGGTCCGGGTTGGGTTTGACATCATTGGCCAGGTTCCAGTTGCTGTTGCCGTCACTTTTCAGGACATACTCATAGGTCCCAAGATCAACGAAACCGCCGGTATTGCCCAGCGTAAACGAAg                          >  2:78650/1‑140 (MQ=255)                                                                                           tGGCTTTGGATTTGGGATGGGGTCCGGGTTGGGTTTGACATCATTGGCCAGGTTCCAGTTGCTGTTGCCGTCACTTTTCAGGACATACTCATAGGTCCCAAGATCAACGAAACCGCCGGTATTGCCCAGCGTAAACGAAg                          >  2:113009/1‑140 (MQ=255)                                                                                                       tgggttgggtGCGGGTTTGGGTTAGACCTCATGGGCCGGGTTCAAGTTGCGGTTGCCGCCATTTTTCGGGACATCCTAATGGGTCCCAAAACCAACGAAACCGCCGGTATTGCCCAGCGTAAACGAAGCAtcccctcccc              <  2:86526/140‑1 (MQ=255)                                                                                                              gggTCCGGGTTGGGTTTGACATCATTGGCCAGGTTCCAGTTGCTGTTGCCGTCACTTTTCAGGACATACTCATAGGTCCCAAGATCAACGAAACCGCCGGTATTGCCCAGCGTAAACGAAGCATCCCCTCCCCCTGtttt       >  1:51661/1‑140 (MQ=255)                                                                                                              gggTCCGGGTTGGGTTTGACATCATTGGCCAGGTTCCAGTTGCTGTTGCCGTCACTTTTCAGGACATACTCATAGGTCCCAAGATCAACGAAACCGCCGGTAATGCCCAGTGTAAACAAAGCATACCCTCCCCCTGtttt       >  1:289947/1‑140 (MQ=255)                                                                                                                   cGGGTTGGGTTTGACATCATTGGCCAGGTTCCAGTTGCTGTTGCCGTCACTTTTCAGGACATACTCATAGGTCCCAAGATCAACGAAACCGCCGGTATTGCCCAGCGTAAACGAAGCATCCCCTCCCCCTGTTTTCACCa  >  1:95140/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                     AATGCGTTTCTCCGGAACGGGTGTCGGCGTTGGATCGGGAGTAGGGTCTGGTTTTGGATTTGGGTCTGGCTTTGGATCGGGTTTTGGGTCTGGCTTTGGATTTGGGATGGGGTCCGGGTTGGGTTTGACATCATTGGTCAGGTTCCAGTTGCTGTTGCCGTCACTTTTCAGGACATACTCATAGGTCCCAAGATCAACGAAACCGCCGGTATTGCCCAGCGTAAACGAAGCATCCCCTCCCCCTGTTTTCACCA  >  NC_000913/2779055‑2779308

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑