Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,811,134	A→G	H122R (CAC→CGC)	mprA →	DNA‑binding transcriptional repressor MprA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,811,134	0	A	G	100.0%	96.0 / NA	28	H122R (CAC→CGC)	mprA	DNA‑binding transcriptional repressor MprA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (11/17);  total (11/17)

TGCTCTTGGATCATCCCGTACCAACGCGACGCGTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCACGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGA  >  NC_000913/2811006‑2811262                                                                                                                                 |                                                                                                                                 tgCTCTTGGATCATCCCGTACCAACGCGACGCGTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATGGCCGCTGCATGCTTCTGCAATTAACCGAAAAAGGTCGCGAGTTTTTgc                                                                                                                       >  2:215339/1‑140 (MQ=255)   ctctTGGATCATCCCGTACCAACGCGACGCGTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAACAGGTCGCGAGTTTTTgcgc                                                                                                                     <  2:385543/140‑1 (MQ=255)   ctctTGGATCATCCCGTACCAACGCGACGCGTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCGCGAGTTTTTgcgc                                                                                                                     <  1:105013/140‑1 (MQ=255)     ctTGGATCATCCCGTACCAACGCGACGCGTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCGCGAGTTTTTGCGCGa                                                                                                                   <  1:536078/140‑1 (MQ=255)             atcCCGTACCAACGCGACGCGTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTac                                                                                                           >  1:147189/1‑140 (MQ=255)              tcCCGTACCAACGCGACGCGTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAGTTAACGGAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTac                                                                                                           >  2:145965/1‑139 (MQ=255)              tcCCGTACCAACGCGACGCGTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTacc                                                                                                          >  1:143768/1‑140 (MQ=255)              tcCCGTACCAACGCGACGCGTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTacc                                                                                                          >  2:151033/1‑140 (MQ=255)              tcCCGTACCAACGCGACGCGTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTac                                                                                                           >  2:27934/1‑139 (MQ=255)              tcCCGTACCAACGCGACGCGTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTac                                                                                                           <  1:145965/139‑1 (MQ=255)              tcCCGTACCAACGCGACGCGTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTac                                                                                                           <  1:27934/139‑1 (MQ=255)                ccGTACCAACGCGACGCGTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCAGCAATTAACGGAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTaccac                                                                                                        <  2:343041/140‑1 (MQ=255)                         cgcgacgcgTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATAGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATa                                                                                               >  2:603402/1‑140 (MQ=255)                          gcgacgcgTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATaa                                                                                              <  1:130594/140‑1 (MQ=255)                             acgcgTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAActg                                                                                           <  2:147189/140‑1 (MQ=255)                                    ttGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCAt                                                                                    <  2:404515/140‑1 (MQ=255)                                    ttGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCAt                                                                                    <  2:1482/140‑1 (MQ=255)                                                            gTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCGCGAGTTTTTGTGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACTCTGGTCCGCGCTCAGcaca                                                            >  2:506100/1‑140 (MQ=255)                                                                           gtGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAg                                             <  1:125491/140‑1 (MQ=255)                                                                            tGAAAGCGATAACGATCGCCGCGGCATGCGTCTGCAATTAACGGAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGc                                            <  1:215339/140‑1 (MQ=255)                                                                                            cgccgcTGCCTGCATCTGCAATTAACGGAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCg                            >  2:129280/1‑140 (MQ=255)                                                                                                   gcctgcATCTGCAATTAACGGATAATGGTTGCGAGTTGTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTg                     <  1:236453/140‑1 (MQ=255)                                                                                                      tgcATCTGCAATTAACGGAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGctc                  >  2:69985/1‑140 (MQ=255)                                                                                                            tGCAATTAACGGAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGt            <  1:597882/140‑1 (MQ=255)                                                                                                                 ttAACGGAAAAAGGTCGCGAGTTTTTGTGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGa       <  1:506100/140‑1 (MQ=255)                                                                                                                 ttAACGGAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCATATTGCTCTCCCGTCTCGa       <  2:143768/140‑1 (MQ=255)                                                                                                                 ttAACGGAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGa       <  1:603402/140‑1 (MQ=255)                                                                                                                      ggAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAAATCAGCTCGAGCAAATAACCCGCAAATTGCCCTCCCGTCTCGACCAGa  >  2:228941/1‑140 (MQ=255)                                                                                                                                 |                                                                                                                                 TGCTCTTGGATCATCCCGTACCAACGCGACGCGTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCACGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGA  >  NC_000913/2811006‑2811262

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑