Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,832,101 T→C V63V (GTA→GTG)  norR ← DNA‑binding transcriptional dual regulator NorR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,832,1010TC96.8% 99.4 / NA 31V63V (GTA→GTG) norRDNA‑binding transcriptional dual regulator NorR
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (17/13);  total (18/13)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGACTCTCCTGCCCAGGAATCAAACCGTCATAGGGATCGGGCAATTCGCTGTCTGCGGGAAAGCGCACCACATCCCCGGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTCCAGCGCAAAGCGTCTACCGAGTACATCCTTTGCCAGACCGTCGATGGCAAGCGGAATAAACTGCCGCGAATCGTAACGTAGCAACGCAGACGCATCGCACTCCAGCACCTGACGTAGCGTGGTGATCAGGCGCTGAAAACGATCCTGGTGAC  >  NC_000913/2831967‑2832231
                                                                                                                                      |                                                                                                                                  
aGACTCTCCTGCCCAGGAATCAAACCGTCATAGGGATCGGGCAATTCGCTGTCTGCGGGAAAGCGCACCACATCCCCGGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTCCAGCGCAAAGCGTCTACCGAGCACATc                                                                                                                               <  2:477625/140‑1 (MQ=255)
      tcCTGCCCAGGAATCAAACCGTCATAGGGATCGGGCAATTCGCTGTCTGCGGGAAAGCGCACCACATCCCCGGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTCCAGCGCAAAGCGTCTACCGAGCACATCCTTTGc                                                                                                                         >  1:485279/1‑140 (MQ=255)
      tcCTGCCCAGGAATCAAACCGTCATAGGGACCGGGCAATTCGCTGTCTGCGGGAAAGCGCACCACATCCCCGGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTCCGGCGCAAAGCGTCTGCCGAGCACATCCTTTGc                                                                                                                         >  2:206850/1‑140 (MQ=255)
            ccAGGAATCAAACCGTCATAGGGATCGGGCAATTCGCTGTCTGCGGGAAAGCGCACCACATCCCCGGCGCGGGCAATCGCTTCCAGCCGTGAATGCCCTTTCAGTGCAGAGCGTCTAGCGAGTACATCCTTTGCCAGAcc                                                                                                                   >  2:176562/1‑140 (MQ=255)
              aGGAATCAAACCGTCATAGGGATCGGGCAATTCGCTGTCTGCGGGAAAGCGCACCACATCCCCGGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTCCAGGGCAAAGCGTCTACCTAGCACATCCTTTGCCAGACCGt                                                                                                                 >  1:289167/1‑140 (MQ=255)
                gAATCAAACCGTCATAGGGATCGGGCAATTCGCTGTCTGCGGGAAAGCGCACCACATCCCCGGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTCCAGCGCAAAGCGTCTACCGAGCACATCCTTTGCCAGACCGTCg                                                                                                               <  1:16764/140‑1 (MQ=255)
                gAATCAAACCGTCATAGGGATCGGGCAATTCGCTGTCTGCGGGAAAGCGCACCACATCCCCGGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTCCAGCGCAAAGCGTCTACCGAGCACATCCTTTGCCAGACCGTCg                                                                                                               >  2:14512/1‑140 (MQ=255)
                gAATCAAACCGTCATAGGGATCGGGCAATTCGCTGTCTGCGGGAAAGCGCACCACATCCCCGGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTCCAGCGCAAAGCGTATACCGACCACATCCTTTGCCAGACCGTCg                                                                                                               >  1:260137/1‑140 (MQ=255)
                       aCCGTCATAGGGATCGGGCAATTCGCTGTCTCCGGGAAACCGCACCACCTCCCCGGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTCCAGCGCAAAGCGTCTACCGAGCACATCCTTTGCCAGACCGTCGATGGCaa                                                                                                        <  2:236711/140‑1 (MQ=255)
                           tCATAGGGATCGGGCAATTCGCTGTCTGCGGGAAAGCGCACCACATCCCCGGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTCCAGCGCAAAGCGTCTACCGAGCACATCCCTTGCCAGACCGTCGATGGCAAGCgg                                                                                                    >  1:295310/1‑140 (MQ=255)
                                     cGGGCAATTCGCTGTCTGCGGGAAAGCGCACCACATCCCCGGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTCCAGCGCAAAGCGTCTACCGAGCACATCCTTTGCCAGACCGTCGATGGCAAGCGGAATAAACTGc                                                                                          >  2:632448/1‑140 (MQ=255)
                                     cGGGCAATTCGCTGTCTGCGGGAAAGCGCACCACATCCCCGGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTCCAGCGCAAAGCGTCTACCGAGCACATCCTTTGCCAGACCGTCGATGGCAAGCGGAATAAACTGc                                                                                          >  2:586312/1‑140 (MQ=255)
                                            ttCGCTGTCTGCGGGAAAGCGCACCACATCCCCGGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTCCAGCGCAAAGCGTCTACCGAGCACATCCTTTGCCAGACCGTCGATGGCAAGCGGAATAAACTGCCGCGAAt                                                                                   <  1:632448/140‑1 (MQ=255)
                                            ttCGCTGTCTGCGGGAAAGCGCACCACATCCCCGGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTCCAGCGCAAAGCGTCTACCGAGCACATCCTTTGCCAGACCGTCGATGGCAAGCGGAATAAACAGCCGCGAAt                                                                                   >  2:387132/1‑140 (MQ=255)
                                               gCTGTCTGCGGGAAAACGCACCCCAACCCAGGCGCGGCCAATCGCTTCCAGCCGTGGATGCCCTTCCAGCGCAAAGCGTCTACCGAGCACATCCTTTGCCAGACCGTCGATGGCAAGCGGAATAAACTGCCGCGAATCGt                                                                                <  1:254752/140‑1 (MQ=255)
                                                  gTCTGCGGGAAAGCGCACCACATCCCCGGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTCCAGCGCAAAGCGTCTACCGAGCACATCCTTTGCCAGACCGTCGATGGCAAGCGGAATAAACTGCCGCGAATCGTAAc                                                                             >  2:94015/1‑140 (MQ=255)
                                                             aGCGCACCACATCCCCGGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTCCAGCGCAAAGCGTCTACCGAGCACATCCTTTGCCAGACCGTCGATGGCAATCGGAATAAACTGCCGCGAATCTTAACGTAGCAACGCa                                                                  >  1:305225/1‑140 (MQ=255)
                                                               cgcACCACATCCCCGGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTCCAGCGCAAAGCGTCTACCGAGCACATCCTTTGCCAGACCGTCGATGGCAAGCGGAATAAACTGCCGCGAATCGTAACGTAGCAACGCAGa                                                                >  1:137640/1‑140 (MQ=255)
                                                                 caccacATCCCCGGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTCCAGCGCAAAGCGTCTACCGAGCACATCCTTTGCCAGACCGTCGATGGCAAGCGGAATAAACTGCCGCGAATCGTAACGTAGCAACGCAGACg                                                              <  1:94015/140‑1 (MQ=255)
                                                                   ccacATCCCCGGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTCCAGCGCAAAGCGTCTACCGAGCACATCCTTTGCCAGACCGTCGATGGCAAGCGGAATAAACTGCCGCGAATCGTAACGTAGCAACGCAGACGCa                                                            <  1:476690/140‑1 (MQ=255)
                                                                              gcgcGGGCAATCGCTTCCAGCCGTGGATGCCCTTCCAGCGCAAAGCGTCTACCGAGCACATCCTTTGCCAGACCGTCGATGGCAAGCGGAATAAACTGCCGCGAATCGTAACGTAGCAACGCAGACGCATCGCACTCCAg                                                 >  2:5219/1‑140 (MQ=255)
                                                                              gcgcGGGCAATCGCTTCCAGCCGTGGATGCCCTTCCAGCGCAAAGCGTCTACCGAGCACATCCTTTGCCAGACCGTCGATGGCAAGCGGAATAAACTGCCGCGAATCGTAACGTAGCAACGCAGACGCATCGCACTCCAg                                                 >  2:618163/1‑140 (MQ=255)
                                                                              gcgcGGGCAATCGCTTCCAGCCGTGGATGCCCTTCCAGCGCAAAGCGTCTACCGAGCACATCCTTTGCCAGACCGTCGATGGCAAGCGGAATAAACTGCCGCGAATCGTAACGTAGCAACGCAGACGCATCGCACTCCAg                                                 >  1:502639/1‑140 (MQ=255)
                                                                                 cgGGCAATCGCTTCCACGCGTGTATGCCCTTACAGCGCACAGCGTCTACCGAGCACATCCTTTGCCAGACCGTCGATGGCAAGCGGAATAAACTGCCGCGAATCGTAACGTAGCAACGCAGACGCATCGCACTCCAGCAc                                              <  2:305225/140‑1 (MQ=255)
                                                                                   ggCAATCGCTTCCAGCCGTGGATGCCCTTCCAGCGCAAAGCGTCTACCGAGCACATCCTTTGCCAGACCGTCGATGGCAAGCGGAATAAACTGCCGCGAATCGTAACGTAGCAACGCAGACGCATCGCACTCCAGCACCt                                            >  1:601877/1‑140 (MQ=255)
                                                                                     cAATCGCTTCCAGCCGTGGATGCCCTTCCAGCGCAAAGCGTCTACCGAGCACATCCTTTGCCAGACCGTCGATGGCAAGCGGAATAAACTGCCGCGAATCGTAACGTAGCAACGCAGACGCATCGCACTCCAGCACCTGa                                          >  2:387339/1‑140 (MQ=255)
                                                                                       aTCGCTTCCAGCCGTGGATGCCCTTCCAGCGCAAAGCGTCTACCGAGCACATCCTTTGCCAGACCGTCGATGGCAAGCGGAATAAACTGCCGCGAATCGTAACGTAGCAACACAGACGCATCGCACTCCAGCACCTGACg                                        <  2:494693/140‑1 (MQ=255)
                                                                                         cGCTTCCAGCCGTGGATGCCCTTCCAGCGCAAAGCGTCTACCGAGCACATCCTTTGCCAGACCGTCGATGGCAAGCGGAATAAACTGCCGCGAATCGTAACGTAGCAACGCAGACGCATCGCACTCCAGCACCTGACGTa                                      <  1:618163/140‑1 (MQ=255)
                                                                                          gCTTCCAGCCGTGGATGCCCTTCCAGCGCAAAGCGTCTACCGAGCACATCCTTTGCCAGACCGTCGATGGCAAGCGGAATAAACTGCCGCGAATCGTAACGTAGCAACGCAGACGCATCGCACTCCAGCACCTGACGTAg                                     <  2:601877/140‑1 (MQ=255)
                                                                                                                             tCTACCGAGCACATCCTTTGCCAGACCGTCGATGGCAAGCGGAATAAACTGCCGCGAATCGTAACGTAGCAACGCAGACGCATCGCACTCCAGCACCTGACGTAGCGTGGTGATCAGGCGCTGAAAACGATCCTGGTGAc  <  2:485279/140‑1 (MQ=255)
                                                                                                                             tCTACCGAGCACATCCTTTGCCAGACCGTCGATGGCAAGCGGAATAAACTGCCGCGAATCGTAACGTAGCAACGCAGACGCATCGCACTCCAGCACCTGACGTAGCGTGGTGATCAGGCGCTGAAAACGATCCTGGTGAc  <  1:586312/140‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                  
AGACTCTCCTGCCCAGGAATCAAACCGTCATAGGGATCGGGCAATTCGCTGTCTGCGGGAAAGCGCACCACATCCCCGGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTCCAGCGCAAAGCGTCTACCGAGTACATCCTTTGCCAGACCGTCGATGGCAAGCGGAATAAACTGCCGCGAATCGTAACGTAGCAACGCAGACGCATCGCACTCCAGCACCTGACGTAGCGTGGTGATCAGGCGCTGAAAACGATCCTGGTGAC  >  NC_000913/2831967‑2832231

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑