Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	31,826	A→G	N337S (AAC→AGC)	carB →	carbamoyl phosphate synthetase subunit beta

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	31,826	0	A	G	100.0%	66.3 / NA	20	N337S (AAC→AGC)	carB	carbamoyl phosphate synthetase subunit beta
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (4/16);  total (4/16)

TGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCACTGGCGGAC‑GTACTCCGGCCTCCTTCGAGCCGTCCATCGACTATGTGGTTACTAAAATTCCTCGCTTCAACTTCGAAAAATTCGCCGGTGCTAACGACCGTCTGACCACTCAGATGAAATCGGTTGG  >  NC_000913/31704‑31961                                                                                                                           |                                                                                                                                         tGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACGCCCTCGACGAACTGATGAGCGACATCACTGGCGGAc                                                                                                                         <  2:330013/140‑1 (MQ=255)         aaaTGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTTATGAGCGACATCACTGGCGGAC‑GGACTccc                                                                                                                >  1:89105/1‑139 (MQ=255)                cccACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAGCGACATCACTGGCGGAC‑GTACTCCGGcctcct                                                                                                         <  2:91374/140‑1 (MQ=255)                  cACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAGCGACATCACTGGCGGAC‑GTACTCCGGCCTCCTTc                                                                                                       <  2:129530/140‑1 (MQ=255)                        tgtCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGGGGGGTACACCCCCGACGGACTGATTAGCGACATCACCGGCGGGCGGAACCCCGGCCCCCtttggccc                                                                                                  >  2:511811/1‑134 (MQ=255)                             cGTTCTTCGGCGCTGGCGTCGACAGCGACCGCTTTCCCGTTTGCTAAACTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAGCGACATCACTGGCGGAC‑GTACTCCGGCCTCCTTCGAGCCGTCCAt                                                                                            <  2:269038/140‑1 (MQ=255)                             cGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAGCGACATCACTGGCGGAC‑GTACTCCGGCCTCCTTCGAGCCGTCCAt                                                                                            <  1:592835/140‑1 (MQ=255)                             cGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAGCGACATCACTGGCGGAC‑GTACTCCGGCCTCCTTCGAGCCGTCCAt                                                                                            <  2:511833/140‑1 (MQ=255)                             cGTTCTTCGGCGCTGGCCTCGACAGCGACCGATTTCCCGGTTGCTAAACTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAGCGACATCACTGGCGGAC‑GTACTCCGGCCTCCTTCGAGCCGTCCAt                                                                                            <  2:201434/140‑1 (MQ=255)                                 cttcGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACCGATGAGCGACATCACTGGCGGAC‑GTACTCCGGCCTCCTTCGAGCCGTCCATCGAc                                                                                        <  1:493792/140‑1 (MQ=255)                                         cTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAGCGACATCACTGGCGGAC‑GTACTCCGGCCTCCTTCGAGCCGTCCATCGACTATGTGGt                                                                                <  1:121522/140‑1 (MQ=255)                                            gCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAGCGACATCACTGGCGGAC‑GTACTCCGGCCTCCTTCGAGCCGTCCATCGACTATGTGGTTAc                                                                             <  1:492019/140‑1 (MQ=255)                                                    aGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAGCGACATCACTGGCGGAC‑GTACTCCGGCCTCCTTCGAGCCGTCCATCGACTATGTGGTTACTAAAATTc                                                                     <  1:60979/140‑1 (MQ=255)                                                    aGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAGCGACATCACTGGCGGAC‑GTACTCCGGCCTCCTTCGAGCCGTCCATCGACTATGTGGTTACTAAAATTc                                                                     <  2:117713/140‑1 (MQ=255)                                                                                             cGGTGGGTTACACCCTCGACGAACTGATGAGCGACATCACTGGCGGAC‑GTACTCCGGCCTCCTTCGAGCCGTCCATCGACTATGTGGTTACTAAAATTCCTCGCTTCAACTTCGAAAAATTCGCCGGTGCTAACGACCGt                            <  1:152082/140‑1 (MQ=255)                                                                                                     tACACCCTCGACGAACTGATGAGCGACATCACTGGCGGAC‑GTACTCCGGCCTCCTTCGAGCCGTCCATCGACTATGTGGTTACTAAAATTCCTCGCTTCAACTTCGAAAAATTCGCCGGTGCTAACGACCGTCTGACCAc                    <  1:511811/140‑1 (MQ=255)                                                                                                       cacCCTCGACGAACTGATGAGCGACATCACTGGCGGAC‑GCACTCCCGCCTCCTTCGAGCCGTCCATCGACTATGTGGTTACTAAAATTCCTCGCTTCAACTTCGAAAAATTCGCCCGTGCTAACGACCGTCTGACCCCTc                  >  1:610091/1‑140 (MQ=255)                                                                                                            tCGACGAACTGATGAGCGACATCACTGGCGGAC‑GTACTCCGGCCTCCTTCGAGCCGTCCATCGACTATGTGGTTACTAAAATTCCTCGCTTCAACTTCGAAAAATTCGCCGGTGCTAACGACCGTCTGACCACTCAGATg             >  2:28704/1‑140 (MQ=255)                                                                                                            tCGACGAACTGATGAGCGACATCACTGGCGGAC‑GTACTCCGGCCTCCTTCGAGCCGTCCATCGACTATGTGGTTACTAAAATTCCTCGCTTCAACTTCGAAAAATTCGCCGGTGCTAACGACCGTCTGACCACTCAGATg             >  2:9037/1‑140 (MQ=255)                                                                                                            tCGACGAACTGATGAGCGACATCACTGGCGGAC‑GTACTCCGGCCTCCTTCGAGCCGTCCATCGACTATGTGGTTACTAAAATTCCTCGCTTAAACTTCGATAAAGTCGGCGGTGCTAGCGACGGTCTGACCACTCAGATg             >  2:208592/1‑140 (MQ=255)                                                                                                                    cTGATGAGCGACATCACTGGCGGAC‑GTACTCCGGCCTCCTTCGAGCCGTCCATCGACTATGTGGTTACTAAAATTCCTCGCTTCAACTTCGAAAAATTCGCCGGTGCTAACGACCGTCTGACCACTCAGATGAAATCGGt     <  1:9037/140‑1 (MQ=255)                                                                                                                       atgaGCGACATCACTGGCGGAC‑GTACTCCGGCCTCCTTCGAGCCGTCCATCGACTATGTGGTTACTAAAATTCCTCGCTTCAACTTCGAAAAATTCGCCGGTGCTAACGACCGTCTGACCACTCAGATGAAATCGGTTgg  <  2:89105/140‑1 (MQ=255)                                                                                                                           |                                                                                                                                         TGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCACTGGCGGAC‑GTACTCCGGCCTCCTTCGAGCCGTCCATCGACTATGTGGTTACTAAAATTCCTCGCTTCAACTTCGAAAAATTCGCCGGTGCTAACGACCGTCTGACCACTCAGATGAAATCGGTTGG  >  NC_000913/31704‑31961

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑