| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 281,529 | T→C | H152R (CAC→CGC) | yagA ← | CP4‑6 prophage; integrase core domain‑containing protein YagA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 281,529 | 0 | T | C | 95.2% | 64.8 / NA | 22 | H152R (CAC→CGC) | yagA | CP4‑6 prophage; integrase core domain‑containing protein YagA |
| Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (8/12); total (9/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGACCAGCTGCTGCTGCACGGTCTCGCGCCGTTCATCGGTACAGTGCGCCAGGCACAGGGAAAAACGGGAGTGGTCGTCCAGCAGGGTGAGCGGATGGCAGCGTCCACCGCCAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGC > NC_000913/281407‑281663 | tGACCAGCTGCTGCTGCACGGTCTCGCGCCGTTCATCGGTACAGTGCGCCAGGCACAGGGAAAAACGGGAGTGGTCGTCCAGCAGGGTGAGGGGATGGCAGCGTGCACGGCCAAAAGGAAAGAGGCCCTTAAAATCCATc > 2:195360/1‑140 (MQ=255) ctgctgcACGGTCTCGCGCCGTTCATCGGTACAGTGCGCCAGGCACAGGGAAAAACGGGAGTGGTCGTCCAGCAGGGTGAGCGGATGGCAGCGTCCACCGCCAAAAGGAAAGCGGCCCTTAAAATCCATCTGCCAGAGGc > 1:475732/1‑140 (MQ=255) ctcGCGCCGTTCATCTGTACAGTGCCCCATGCACAGGCAAAAACGGGAGTGGTCGTCCAGCAGGGTGAGCGGATGGCAGCGTCCACCGCCAAAAGGAAAGCGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGcgt < 1:195360/140‑1 (MQ=255) ctcGCGCCGTTCATCGGTACAGTGCGCCAGGCACAGGGAAAAACGGGAGTGGTCGTCCAGCAGGGTGAGCGGATGGCAGCGTCCACCGCCAAAAGGAAAGCGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGcgt < 2:69375/140‑1 (MQ=255) tcccgcCGTTCATCGGTATAGTGCGCCAGGCAGAGCGAGAAACGGGAGTGGTCGTCCAGCAGAGTGAGCGCATGGCAGCGTCCCCCGCCAAAAGGAAAGCGGCCCTTAAAATCCATATGCCAGAGGCGGTTCGGCGcgtc < 2:583276/137‑1 (MQ=255) ttCATCGGTACAGTGCGCCAGGCACAGGGAAAAACGGGAGTGGTCGTCCAGCAGGGTGAGCGGATGGCAGCGTCCACCGCCAAAAGGAAAGCGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGa > 1:114919/1‑140 (MQ=255) ggTACAGTGCGCCAGGCACAGGGAAAAACGGGAGTGGTCGTCCAGCAGGGTGAGCGGATGGCAGCGTCCACCGCCAAAAGGAAAGCGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGc < 2:475732/140‑1 (MQ=255) aGTGCGCCAGGCACAGGGAAAAACGGGAGTGGTCGTCCAGCAGGGTGAGCGGATGGCAGCGTCCACCGCCAAAAGGAAAGCGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGGGTTCGAACCGGCCCGTg > 2:435843/1‑140 (MQ=255) tGCGCCAGGCACAGGGAAAAACGGGAGTGGTCGTCCAGCAGGGTGAGCGGATGGCAGCGTCCACCGCCAAAAGGAAAGCGGCCCTTAAAATGCATCTGCCGGAGGCGGGTCGGCGCGGCGTGATCGAACCGGCCCGTggg > 2:185164/1‑139 (MQ=255) tGCGCCAGGCACAGGGAAAAACGGGAGTGGTCGTCCAGCAGGGTGAGCGGATGGCAGCGTCCACCGCCAAAAGGAAAGCGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGc > 2:376022/1‑140 (MQ=255) cgcCAGGCACAGGGAAAAACGGGAGTGGTCGTCCAGCAGGGTGAGCGGATGGCAGCGTCCACCGCCAAAAGGAAAGCGGCCCTTAAAATCCTTCTGCCAGGGGCGGGTAGCCGCGCCACGTTTGAACAGGCCCGGTGCgg > 2:169514/1‑140 (MQ=255) cgcCAGGCACAGGGAAAAACGGGAGTGGTCGTCCAGCAGGGTGAGCGGATGGCAGCGTCCACCGCCAAAAGGAAAGCGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCgg > 1:43483/1‑140 (MQ=255) cgcCAGGCACAGGGAAAAACGGGAGTGGTCGTCCAGCAGGGTGAGCGGATGGCAGCGTCCACCGCCAAAAGGAAAGCGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCCTGGCgg > 2:20629/1‑140 (MQ=255) cgcCAGGCACAGGGAAAAACGGGAGTGGTCGTCCAGCAGGGTGAGCGGATGGCAGCGTCCACCGCCAAAAGGAAAGCGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGGCGTGTTCGAACCCGCCCGGGGCgg > 2:375835/1‑140 (MQ=255) ccAGGCACAGGGAAAAACGGGAGTGGTCGTCCAGCAGGGTGAGCGGATGGCAGCGTCCACCGCCAAAAGGAAAGCGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGa < 2:114919/140‑1 (MQ=255) aaaaCGGGAGTGGTCGTCCAGCAGGGTGAGCAGATCGCATCGTCCACCACCAAAAGGAAAGCGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCCCGGTGAAg < 2:242652/140‑1 (MQ=255) tGGTCGTCCAGCAGGGTGAGCGGATGGCAGCGTCCACCGCCAAAAGGAAAGCGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGgcag < 2:566187/140‑1 (MQ=255) ggCGGATGACAGCGTCGTCCGCAAAAAGGATGGTGGCCCATAAAATCCTTCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCAt < 2:325933/139‑1 (MQ=255) gCGGATGGCGGCGTCCACCGCCAAAAGGAAAGCGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATc < 2:483637/140‑1 (MQ=255) gCGGATGGCAGCGTCTACCGCCAAACGGACAGCGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATc < 1:185164/140‑1 (MQ=255) gCGGATGGCAGCGTCCACCGCCAAAAGGAAAGCGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATc < 2:68811/140‑1 (MQ=255) gCGTCCACCGCCAAAAGGAAAGCGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGa < 2:140257/140‑1 (MQ=255) aaaaGGAAAGCGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAgg < 1:376022/140‑1 (MQ=255) gAAAGCGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGc < 1:375835/140‑1 (MQ=255) | TGACCAGCTGCTGCTGCACGGTCTCGCGCCGTTCATCGGTACAGTGCGCCAGGCACAGGGAAAAACGGGAGTGGTCGTCCAGCAGGGTGAGCGGATGGCAGCGTCCACCGCCAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGC > NC_000913/281407‑281663 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |