Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,845,589	T→C	T295A (ACC→GCC)	hycE ←	formate hydrogenlyase subunit HycE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,845,589	0	T	C	100.0%	71.8 / NA	21	T295A (ACC→GCC)	hycE	formate hydrogenlyase subunit HycE
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (11/10);  total (11/10)

TCTTTCAGCAGATCGCGACGAATCCCGCCGATCAAGTTCAGGCCGTAGGTTTTACGCGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGGAAGAACTGCATAAAGCCGGAGTCGAAGCCGGTAAAGTGACAGGCCAGGCCAAGGTTGAGCAGATGCGAGTGCAAGCGTTCTACCTCCAGCAGAATGGCGCGGATCATCTGCGCACGTTCTGGCACCTGAATACCCATCGCGTTTTCCA  >  NC_000913/2845453‑2845705                                                                                                                                         |                                                                                                                     tcTTTCAGCAGATCGCGACGAATCCCGCCGATCAAGTTCAGGCCGTAGGTTTTACGCGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGGAAGAACTGCATAAAGCCGGAGTCGAAGCCGGCaaa                                                                                                                   >  1:431117/1‑140 (MQ=255) tcTTTCAGCAGATCGCGACGAATCCCGCCGATCAAGTTCAGGCCGTAGGTTTTACGCGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGGAAGAACTGCATAAAGCCGGAGTCGAAGCCGGCaaa                                                                                                                   >  2:419130/1‑140 (MQ=255)     tCAGCAGATCGCGACGAATCCCCCCGATCAAGTTCAGTCCGTAGGTTTTACGCGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGGAAGAACTGCATAAAGCCGGAGTCGAAGCCGGCAAAGTGa                                                                                                               <  1:281940/140‑1 (MQ=255)     tCAGCAGATCGCGACAATTCCCCCCGATCAACTTCAGGCAGTAGGTTTTACGCGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGGAAGAACTGCATAAAGCCGGAGTCGAAGCCGGCAAAGTGa                                                                                                               <  2:212388/140‑1 (MQ=255)     tCAACAGATCGGGACAACCCCCGCCGATCAAGTTCAGGCCGTAGGTTTTACGCGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGGAAGAACTGCATAAAGCCGGAGTCGAAGCCGGCAAAGTGa                                                                                                               <  2:138827/140‑1 (MQ=255)        gcagATCGCGACGAATCCCGCCGATCAAGTTCAGGCCGTAGGTTTTACGCGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGGAAGAACTGCATAAAGCCGGAGACGAAGCCGGCAAAGTGAcag                                                                                                            >  1:541560/1‑140 (MQ=255)          agATCGCGACGAATCCCGCCGATCAAGTTCAGGCCGTAGGTTTTACGCGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGGAAGAACTGCATAAAGCCGGAGTCGAAGCCGGCAAAGTGAcaggc                                                                                                          <  2:448542/140‑1 (MQ=255)          agACCGGGACGAACCCCGCCGATCAAGTTCAGGCCGTAGGTTTTACGCGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGGAAGAACTGCATAAAGCCGGAGTCGAAGCCGGCAAAGTGAcaggc                                                                                                          <  2:442858/140‑1 (MQ=255)          agACCGCGACGAACCCCGCCGATCAAGTTCAGGCCGTAGGTTTTACGCGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGGAAGAACTGCATAAAGCCGGAGTCGAAGCCGGCAAAGTGAcaggc                                                                                                          <  2:505155/140‑1 (MQ=255)              cgcgACGAATCCCGCCGATCAAGTTCAGGCCGTAGGTTTTACGCGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGGAAGAACTGCATAAAGCCGGAGTCGAAGCCGGCAAAGTGAcaggccagg                                                                                                      >  1:138827/1‑140 (MQ=255)              cgcgACGAATCCCGCCGATCAAGTTCAGGCCGTAGGTTTTACGCGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGGAAGAACTGCATAAAGCCGGAGTCGAAGCCGGCAAAGTGAcaggccagg                                                                                                      >  1:422216/1‑140 (MQ=255)                        cccGCCGATCAAGTTCAGGCCGTAGGTTTTACGCGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGGAAGAACTGCATAAAGCCGGAGTCGAAGCCGGCAAAGTGACAGGCCAGGCCAAGGTTGa                                                                                            <  1:15344/140‑1 (MQ=255)                                      tCAGGCCGTAGGTTTTACGCGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGGAAGAACTGCATAAAGCCGGAGTCGAAGCCGGCAAAGTGACAGGCCAGGCCAAGGTTGAGCAGATGCGAGTGc                                                                              <  1:610003/140‑1 (MQ=255)                                         ggCCGTAGGTTTTACGCGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGGAAGAACTGCATAAAGCCGGAGTCGAAGCCGGCAAAGTGACAGGCCAGGCCAAGGTTGAGCAGATGCGAGTGCAAg                                                                           >  2:99893/1‑140 (MQ=255)                                          gCCGTAGGTTTTACGCGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGGAAGAACTGCATAAAGCCGGAGTCGAAGCCGGCAAAGTGACAGGCCAGGCCAAGGTTGAGCAGATGCGAGTGCAAGc                                                                          >  2:569539/1‑140 (MQ=255)                                                               ggTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGGAAGAACTGCATAAAGCCGGAGTCGAAGCCGGCAAAGTGACAGGCCAGGCCAAGGTTGAGCAGATGCGAGTGCAAGCGTTCTACCTCCAGCAGAATgg                                                     <  2:512191/140‑1 (MQ=255)                                                                               aTTTTCATGGAGGTTTCACGCACGCGGAAGAACTGCATAAAGCCGGAGTCGAAGCCGGCAAAGTGACAGGCCAGGCCAAGGTTGAGCAGATGCGAGTGCAGGCGATTTAGCTCCAGCTGAATAGCGCGGATAATCTgcgc                                     >  2:313798/1‑140 (MQ=255)                                                                               aTTTTCATGGAGGTTTCACGCACGCGGAAGAACTGCATAAAGCCGGAGTCGAAGCCGGCAAAGTGACAGGCCAGGCCAAGGTTGAGCAGATGCGAGTGCAAGCGTTCTACCTCCAGCAGAATGGCGCGGATCATCTgcgc                                     >  2:590731/1‑140 (MQ=255)                                                                                                       cgGAAGAACTGCATAAAGCCGGAGTCGAAGCCGGCAAAGTGACAGGCCAGGCCAAGGTTGAGCAGATGCGAGTGCAAGCGTTCTACCTCCAGCAGAATGGCGCGGATCATCTGCGCACGTTCTGGCACCTGAATACCCAt             >  1:83943/1‑140 (MQ=255)                                                                                                                tGCATAAAGCCGGAGTCGAAGCCGGCAAAGTGACAGGCCAGGCCAAGGTTGAGCAGATGCGAGTGCAAGCGTTCTACCTCCAGCAGAATGGCGCGGATCATCGGCGCACGATCTAGCTCCTGAATACCCATCGCGTTTTc    >  1:312651/1‑140 (MQ=255)                                                                                                                  cATAAAGCCGGAGTCGAAGCCGGCAAAGTGACAGGCCAGGCCAAGGTTGAGCAGATGCGAGTGCAAGCGTTCTACCTCCAGCAGAATGGCGCGGATCATCTGCGCACGTTCTGGCACCTGAATACCCATCGCGTTTTCCa  <  1:462212/140‑1 (MQ=255)                                                                                                                  cATAAAGCCGGAGTCGAAGCCGGCAAAGTGACAGGCCAGGCCAAGGTTGAGCAGATGCGAGTGCAAGCGTTCTACCTCCAGCAGAATGGCGCGGATCATCTGCGCACGTTCTGGCACCTGAATACCCATCGCGTTTTCCa  >  2:653275/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                     TCTTTCAGCAGATCGCGACGAATCCCGCCGATCAAGTTCAGGCCGTAGGTTTTACGCGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGGAAGAACTGCATAAAGCCGGAGTCGAAGCCGGTAAAGTGACAGGCCAGGCCAAGGTTGAGCAGATGCGAGTGCAAGCGTTCTACCTCCAGCAGAATGGCGCGGATCATCTGCGCACGTTCTGGCACCTGAATACCCATCGCGTTTTCCA  >  NC_000913/2845453‑2845705

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑