Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,863,999	T→C	V136A (GTG→GCG)	ygbL →	putative 3‑oxo‑tetronate 4‑phosphate decarboxylase YgbL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,863,999	0	T	C	100.0%	60.3 / NA	18	V136A (GTG→GCG)	ygbL	putative 3‑oxo‑tetronate 4‑phosphate decarboxylase YgbL
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (13/5);  total (13/5)

CCGCGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTTTCCTGCCTGCAAGGGCTGGACAGCAGCAACGTTATTCGTCCGTTCACACCATACGTGGTGATGCGGATGGGAAATGTCCCGCTGGTGCCTTATTACCGACCGGGCGATAAACGCATCGCACAGGATCTGGCGGAACTGGCAGCAGACAATCAGGCTTTTTTACTGGCAAATCATGGCCCAGTGGTTTGCGGTGAAAGCCTGCAAGAAGCCGCCAACAATAT  >  NC_000913/2863863‑2864134                                                                                                                                         |                                                                                                                                        ccGCGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTTTCCTGCCTGCAAGGGCTGGACAGCAGCAACGTTATTCGTCCGTTCACACCATACGTGGTGATGCGGATGGGAAATGTCCCGCTGGCGcc                                                                                                                                      <  1:515883/140‑1 (MQ=255) ccGCGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTTTCCTGCCTGCAAGGGCTGGACAGCAGCAACGTTATTCGTCCGTTCACACCATACGTGGTGATGCGGATGGGAAATGTCCCGCTGGCGcc                                                                                                                                      <  1:534380/140‑1 (MQ=255)                 tggtgCATTTGCACAGCACATGGTCGACGGCGCTTTCCTGCCTGCAAGGGCTGGACAGCAGCAACGTTATTCGTCCGTTCACACCATACGTGGTGATGCGGAGGGGAAATGTCCCGCAGGCGCCGTATTACCGACCGGGc                                                                                                                      >  1:202980/1‑140 (MQ=255)                 tggtgCATTTGCACAGCACATGGTCGACGGCGCTTTCCTGCCTGCAAGGGCTGGACAGCAGCAACGTTATTCGTCCGTTCACACCATACGTGGTGATGCGATTGAGAAATGTCCCGCAGGCGGCTTATTACCGACCGGGc                                                                                                                      >  2:210268/1‑140 (MQ=255)                                                   ttCCTGCCTGCAAGGGCTGGACAGCAGCAACGTTATTCGTCCGTTCACACCATACGTGGTGATGCGGATGGGAAATGTCCCGCTGGCGCCTTATTACCGACCGGGCGATAAACGCATCGCACAGGATCTGGCGGAACTgg                                                                                    <  2:132773/140‑1 (MQ=255)                                                              aaGGGCTGGACAGCAGCAACGTTATTCGTCCGTTCACACCATACGTGGTGATGCGGATGGGAAATGTCCCGCTGGCGCCTTATTACCGACCGGGCGATAAACGCATGGGTCAGGATCTGGCGAAACTGGCAGCAGACAAt                                                                         >  2:317805/1‑140 (MQ=255)                                                                   cTGGACAGCAGCAACGTTATTCGTCCGTTCACACCATACGTGGTGATGCGGATGGGAAATGTCCCGCTGGCGCCTTATTACCGACCGGGCGATAAACGCATCGCACAGGATCTGGCGGAACTGGCAGCAGACAATCAGGc                                                                    >  2:87888/1‑140 (MQ=255)                                                                                           ccGTTCACACCATACGTGGTGATGCGGATGGGAAATGTCCCGCTGGCGCCTTATTACCGACCGGGCGATAAACGCATCGCACAGGATCTGGCGGAACTGGCAGCAGACAATCAGGCTTTTTTACTGGCAAATCATGGccc                                            >  2:561823/1‑140 (MQ=255)                                                                                            cGTTCACACCATACGTGTTGATTCGGAGGGGAAATTTCCCGCTGGCGCTTTATTACCGACCGGGCGATAAACGCATCGCACAGGATCTGGCGGAACTGGCAGCAGACAATCAGGCTTTTTTACTGGCAAATCATGGCCCa                                           <  2:202323/140‑1 (MQ=255)                                                                                                       tACGTGGTGATGCGGATGGGAAATGTCCCGCTGGCGCCTTATTACCGACCGGGCGATAAACGCATCGCACAGGATCTGGCGGAACTGGCAGCAGACAATCAGTCTTTTTTTCTGGCAAATCATGGCCCAGTGGTTTGCgg                                >  1:288895/1‑140 (MQ=255)                                                                                                        aCGTGGTGATGCGGATGGGAAATGTCCCGCTGGCGCCTTATTACCGACCGGGCGATAAACGCATCGCACAGGATCTGGCGGAACTGGCAGCAGACAATCAGGCTTTTTTACTGGCAAATCATGGCCCAGTGGTTTGCGGt                               >  2:386127/1‑140 (MQ=255)                                                                                                              tgATGCGGATGGGAACTGTCCCGCTAGCGCCTTATTACCGACCGGGCGATAAACGCATCGCACAGGATCTGGCGGAACTGGCAGCAGACAATCAGGCTTTTTTACTGGCAAATCATGGCCCAGTGGTTTGCGGTGAAAGc                         <  1:317805/140‑1 (MQ=255)                                                                                                                 tGCGGATGGGAAATGTCCCGCTGGCGCCTTATTACCGACCGGGCGATAAACGCATCGCACAGGATCTGGCGGAACTGGCAGCAGACAATCAGGCTTTTTTACTGGCAAATCATGGCCCAGTGGTTTGCGGTGAAAGCCTg                      >  1:160018/1‑140 (MQ=255)                                                                                                                 tGCGGATGGGAAATGTCCCGCTGGCGCCTTATTACCGACCGGGCGATAAACGCATCGCACAGGATCTGGCGGAACTGGCAGCAGACAATCAGGCTTTTTTACTGGCAAATCATGGCCCAGTGGTTTGCGGTGAAAGCCTg                      >  2:88057/1‑140 (MQ=255)                                                                                                                 tGCGGATGGGAAATGTCCCGCTGGCGCCTTATTACCGACCGGGCGATAAACGCATCGCACAGGATCTGGCGGAACTGGCAGCAGACAATCAGGCTTTTTTACTGGCAAATCATGGCCCAGTGGTTTGCGGTGAAAGCCTg                      >  1:363220/1‑140 (MQ=255)                                                                                                                 tGCGGATGGGAAATGTCCCGCTGGCGCCTTATTACCGACCGGGCGATAAACGCATCGCACAGGATCTGGCGGAACTGGCAGCAGACAATCAGGCTTTTTTACTGGCAAATCATGGCCCAGTGGTTTGCGGTGAAAGCCTg                      >  1:115892/1‑140 (MQ=255)                                                                                                                             aTGTCCCGCTGGCGCCTTATTACCGACCGGGCGATAAACGCATCGCACAGGATCTGGCGGAACTGGCAGCAGACAATCAGGCTTTTTTACTGGCAAATCATGGCCCAGTGGTTTGCGGTGAAAGCCTGCAAGAAgccgcc          <  1:561823/140‑1 (MQ=255)                                                                                                                                tCCCGCTGGCGCCTTATTACCGACCGGGCGATAAACGCATCGCACAGGATCTGGCGGAACTGGCAGCAGACAATCAGGCTTTTTTACTGGCAAATCATGGCCCAGTGGTTTGCGGTGAAAGCCTGCAAGAAgccgcc          <  2:53007/137‑1 (MQ=255)                                                                                                                                tCCCGCTGGCGCCTTATTACCGACCGGGCGATAAACGCATCGCACAGGATCTGGCGGAACTGGCAGCAGACAATCAGGCTTTTTTACTGGCAAATCATGGCCCAGTGGTTTGCGGTGAAAGCCTGCAAGAAgccgcc          >  1:53007/1‑137 (MQ=255)                                                                                                                                     cTGGCGCCTTATTACCGACCGGGCGATAAACGCATCGCACAGGATCTGGCGGAACTGGCAGCAGACAATCAGGCTTTTTTACTGGCAAATCATGGCCCAGTGGTTTGCGGTGAAAGCCTGCAAGAAGCCGCCAACAatat  >  1:560995/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                        CCGCGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTTTCCTGCCTGCAAGGGCTGGACAGCAGCAACGTTATTCGTCCGTTCACACCATACGTGGTGATGCGGATGGGAAATGTCCCGCTGGTGCCTTATTACCGACCGGGCGATAAACGCATCGCACAGGATCTGGCGGAACTGGCAGCAGACAATCAGGCTTTTTTACTGGCAAATCATGGCCCAGTGGTTTGCGGTGAAAGCCTGCAAGAAGCCGCCAACAATAT  >  NC_000913/2863863‑2864134

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑