Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,891,531	T→C	E123G (GAA→GGA)	cysJ ←	sulfite reductase, flavoprotein subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,891,531	0	T	C	100.0%	99.7 / NA	32	E123G (GAA→GGA)	cysJ	sulfite reductase, flavoprotein subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (16/16);  total (16/16)

ACTGGCAGAAAAATTCATAAGAGCTATCGCCGAGGCTAAACACGGCAAACGCGGTGTTTTCCAGCTTTGGCGCTTTTTTGGAGAACAGGAACTTATGCAGCGCGACGGCTTCTTCCGGCGGTTCCCCTTCCCCTTGCGTTGACGTCACTACGATGAGCAGTTTTTCGCTGGCGATTTGTTTGAATTTATAGTCGCCCGCGTTCACCAGCTTAACGTTCAGTTTTGCTGCTAATAAATCATCACGTAATGCTTCAGCA  >  NC_000913/2891409‑2891665                                                                                                                           |                                                                                                                                       aCTGGCAGAAAAATTCATAAGAGCTATCGCCGAGGCTAAACACGGCAAACGCGGTGTTTTCCAGCTTTGGCGCTTTTTTGGAGAACAGGAACTTATGCAGCGCGACGGCTTCTTCCGGCGGTCCCCCTTCCCCTTGCGtt                                                                                                                       <  2:603150/140‑1 (MQ=255) aCTGGCAGAAAAATTCATAAGAGCTATCGCCGAGGCTAAACACGGCAAACGCGGTGTTTTCCAGCTTTGGCGCTTTTTTGGAGAACAGGAACTTATGCAGCGCGACGGCTTCTTCCGGCGGTCCCCCTTCCCCTTGCGtt                                                                                                                       >  2:525466/1‑140 (MQ=255)   tGGCAGAAAAATTCATAAGAGCTATCGCCGAGGCTAAACACGGCAAACGCGGTGTTTTCCAGCTTTGGCGCTTTTTTGGAGAACAGGAACTTATGCAGCGCGACGGCTTCTTCCGGCGGTCCCCCTTCCCCTTGCGTTGa                                                                                                                     <  2:553700/140‑1 (MQ=255)          aaaaTTCATAAGAGCTATCGCCGAGGCTAAACACGGCAAACGCGGTGTTTTCCAGCTTTGGCGCTTTTTTGGAGAACAGGAACTTATGCAGCGCGACGGCTTCTTCCGGCGGTCCCCCTTCCCCTTGCGTTGACGTCACt                                                                                                              >  2:572288/1‑140 (MQ=255)                  tAAGAGCTATCGCCGTGGCTAAACATGGCTAACGCGGTGTTTTCCAGCTTTGGCGCTTTTTTGGAGAACAGGAACTTATGCAGCGCGACGGCTTCTTCCGGCGGTCCCCCTTCCCCTTGCGTTGACGTCACTACGATGAg                                                                                                      <  1:282912/140‑1 (MQ=255)                   aaGAGCTATCGCCGAGGCTAAACACGGCAAACGCGGTGTTTTCCAGCTTTGGCGCTTTTTTGGAGAACAGGAACTTATGCAGCGCGACGGCTTCTTCCGGCGTTCCCCCTGCCCTTTGCGTTGAGGTCACTACGATGAGc                                                                                                     >  1:250467/1‑140 (MQ=255)                   aaGAGCTATCGCCGAGGCTAAACACGGCAAACGCGGTGTTTTCCAGCTTTGGCGCTTTTTTGGAGAACAGGAACTTATGCAGCGCGACGGCTTCTTCCGGCGGTCCCCCTTCCCCTTGCGTTGACGTCACTACGATGAGc                                                                                                     >  1:13822/1‑140 (MQ=255)                                   gCTAAACACGGCAAACGCGGTGTTTTCCAGCTTTGGCGCTTTTTTGGAGAACAGGAACTTATGCAGCGCGACGGCTTCTTCCGGCGGTCCCCCTTCCCCTTGCGTTGACGTCACTACGATGAGCAGTTTTTCGCTGGCGa                                                                                     <  1:374906/140‑1 (MQ=255)                                         cacaGCAAACGCGGTATTTTCCTGCATTGGCGCTTTTTTGGAGAACAGGAACTTATGCAGCGCGACGGCTTCTTCCGGCGGTCCCCCTTCCCCTTGCGTTGACGTCACTACGATGAGCAGTTTTTCGCTGGCGAtttgtt                                                                               <  1:280556/140‑1 (MQ=255)                                         cacGGCAAACGCGGTGTTTTCCAGCTTTGGCGCTTTTTTGGAGAACAGGAACTTATGCAGCGCGACGGCTTCTTCCGGCGGTCCCCCTTCCCCTTGCGTTGACGTCACTACGATGAGCAGTTTTTCGCTGGCGAtttgtt                                                                               <  2:626096/140‑1 (MQ=255)                                         cacGGCAAACGCGGTGTTTTCCAGCTTTGGCGCTTTTTTGGAGAACAGGAACTTATGCAGCGCGACGGCTTCTTCCGGCGGTCCCCCTTCCCCTTGCGTTGACGTCACTACGATGAGCAGTTTTTCGCTGGCGAtttgtt                                                                               <  2:16595/140‑1 (MQ=255)                                             gCAAACGCGGTGTTTTCCAGCTTTGGCGCTTTTTTGGAGAACAGGAACTTATGCAGCGCGACGGCTTCTTCCGGCGGTCCCCCTTCCCCTTGCGTTGACGTCACTACGATGAGCAGTTTTTCGCTGGCGATTTGTTTGaa                                                                           >  2:611466/1‑140 (MQ=255)                                             gCAAACGCGGTGTTTTCCAGCTTTGGCGCTTTTTTGGAGAACAGGAACTTATGCAGCGCGACGGCTTCTTCCGGCGGTCCCCCTTCCCCTTGCGTTGACGCCACGACGATGAGCAGTCTTTCCCTGGCGATTTGTTTGaa                                                                           >  2:253581/1‑140 (MQ=255)                                                      gtgtTTTCCAGCTTTGGCGCTTTTTTGGAGAACAGGAACTTATGCAGCGCGACGGCTTCTTCCGGCGGTCCCCCTTCCCCTTGCGTTGACGTCACTACGATGAGCAGTTTTTCGCTGGCGATTTGTTTGAATTTATAGTc                                                                  >  1:350874/1‑140 (MQ=255)                                                           ttCCAGCTTTGGCGCTTTTTTGGAGAACAGGAACTTATGCAGCGCGACGGCTTCTTCCGGCGGTCCCCCTTCCCCTTGCGTTGACGTCACTACGATGAGCAGTTTTTCGCTGGCGATTTGTTTGAATTTATAGTCGCCcg                                                             >  2:68729/1‑140 (MQ=255)                                                             ccAGCTTTGGCGCTTTTTTGGAGAACAGGAACTTATGCAGTGCGACGGCTTCTTCCGGCGGTCCCCCTTCCCCTTGCGTTGACGTCACTACGATGAGCAGTTTTTCGCTGGCGATTTGTTTGAATTTATAGTCGCCcgcg                                                           <  1:576544/140‑1 (MQ=255)                                                             ccAGCTTTGGCGCTTTTTTGGAGAACAGGAACTTATGCAGCGCGACGGCTTCTTCCGGCGGTCCCCCTTCCCCTTGCGTTGACGTCACTACGATGAGCAGTTTTTCGCTGGCGATTTGTTTGAATTTATAGTCGCCcgcg                                                           <  1:525466/140‑1 (MQ=255)                                                                gCTTTGGCGCTTTTTCGGAGAATAGCAACCTATGCAGCGCGACGGCTTCTTCCGGCGGTCCCCCTTCCCCTTGCGTTGACGTCACTACGATGAGCAGTTTTTCGCTGGCGATTTGTTTGAATTTATAGTCGCCCGCGTTc                                                        <  1:173035/140‑1 (MQ=255)                                                                 cTTTGGCGCTTTTTTGGAGAACAGGAACTTATGCAGCGCGACGGCTTCTTCCGGCGGTCCCCCTTCCCCTTGCGTTGACGTCACTACGATGAGCAGTTTTTCGCTGGCGATTTGTTTGAATTTATAGTCGCCCGCGTTCa                                                       >  2:19206/1‑140 (MQ=255)                                                                 cTTTGGCGCTTTTTTGGAGAACAGGAACTTATGCAGCGCGACGGCTTCTTCCGGCGGTCCCCCTTCCCCTTGCGTTGACGTCACTACGATGAGCAGTTTTTCGCTGGCGATTTGTTTGAATTTATAGTCGCCCGCGTTCa                                                       >  1:507151/1‑140 (MQ=255)                                                                    tGGCGCTTTTTTGGAGAACAGGAACTTATGCAGCGCGACGGCTTCTTCCGGCGGTCCCCCTTCCCCTTGCGTTGACGTCACTACGATGAGCAGTTTTTCGCTGGCGATTTGTTTGAATTTATAGTCGCCCGCGTTCACCa                                                    <  2:51227/140‑1 (MQ=255)                                                                                      cAGGAACTTATGCAGCGCGACGGCTTCTTCCGGCGGTCCCCCTTCCCCTTGCGTTGACGTCACTACGATGAGCAGTTTTTCGCTGGCGATTTGTTTGAATTTATAGTCGCCCGCGTTCACCAGCTTAACGTTCAGTTTtg                                  <  1:572288/140‑1 (MQ=255)                                                                                      cAGGAACTTATGCAGCGCGACGGCTTCTTCCGGCGGTCCCCCTTCCCCTTGCGTTGACGTCACTACGATGAGCAGTTTTTCGCTGGCGATTTGTTTGAATTTATAGTCGCCCGCGTTCACCAGCTTAACGTTCAGTTTtg                                  <  1:397101/140‑1 (MQ=255)                                                                                      cAGGAACTTATGCAGCGCGACGGCTTCTTCCGGCGGTCCCCCTTCCCCTTGCGTTGACGTCACTACGATGAGCAGGTTTTCGCTGGCGATTTGTTTGAATTTATAGTCGCCCGCGTTCACCAGCTTAACGTTCAGTTTtg                                  >  2:384131/1‑140 (MQ=255)                                                                                                    gcgcGACGGCTTCTTCCGGCGGTCCCCCTTCCCCTTGCGTTGACGTCACTACGATGAGCAGTTTTTCGCTGGCGATTTGTTTGAATTTATAGTCGCCCGCGTTCACCAGCTTAACGTTCAGTTTTGCTGCTAATAAatca                    >  2:502965/1‑140 (MQ=255)                                                                                                     cgcgACGGCTTCTTCCGGCGGTCCCCCTTCCCCTTGCGTTGACGTCACTACGATGAGCAGTTTTTCGCTGGCGATTTGTTTGAATTTATAGTCGCCCGCGTTCACCAGCTTAACGTTCAGTTTTGCTGCTAATAAatcat                   <  1:19206/140‑1 (MQ=255)                                                                                                     cgcgACGGCTTCTTCCGGCGGTCCCCCTTCCCCTTGCGTTGACGTCACTACGATGAGCAGTTTTTCGCTGGCGATTTGTTTGAATTTATAGTCGCCCGCGTTCAACAGTTTAACGTTCAGTTTTGCTGCTAATAAatcat                   >  2:224787/1‑140 (MQ=255)                                                                                                       cgACGGCTTCTTCCGGCGGTCCCCCTTCCCCTTGCGTTGACGTCACTACGATGAGCAGTTTTTCGCTGGCGATTTGTTTGAATTTATAGTCGCCCGCGTTCACCAGCTTAACGTTCAGTTTTGCTGCTAATAAAtcatca                 >  2:14333/1‑140 (MQ=255)                                                                                                       cgACGGCTTCTTCCGGCGGTCCCCCTTCCCCTTGCGTTGACGTCACTACGATGAGCAGTTTTTCGCTGGCGATTTGTTTGAATTTATAGTCGCCCGCGTTCACCAGCTTAACGTTCAGTTTTGCTGCTAATAAAtcatca                 >  2:512333/1‑140 (MQ=255)                                                                                                          cGGCTTCTTCCGGCGGTCCCCCGTCCCCTTGGGTTGACGTCACTACGAGGAGCAGTTTTTCGCTGGCGATTTGTTTGAATTTATAGTCGCCCGCGTTCACCAGCTTAACGTTCAGTTTTGCTGCTAATAAATCATCACGt              <  2:250467/140‑1 (MQ=255)                                                                                                            gCTTCTTCCGGCGGTCCCCCTTCCCCTTGCGTTGACGTCACTACGATGAGCAGTTTTTCGCTGGCGATTTGTTTGAATTTATAGTCGCCCGCGTTCACCAGCTTAACGTTCAGTTTTGCTGCTAATAAATCATCACGTaa            <  1:68729/140‑1 (MQ=255)                                                                                                                      gcggTCCCCCTTCCCCTTGCGTTGACGTCACTACGATGAGCAGTTTTTCGCTGGCGATTTGTTTGAATTTATAGTCGCCCGCGTTCACCAGCTTAACGTTCATTTTTGCTGCTAATAAATCATCACGTAATGCTTCAGCa  >  1:229326/1‑140 (MQ=255)                                                                                                                           |                                                                                                                                       ACTGGCAGAAAAATTCATAAGAGCTATCGCCGAGGCTAAACACGGCAAACGCGGTGTTTTCCAGCTTTGGCGCTTTTTTGGAGAACAGGAACTTATGCAGCGCGACGGCTTCTTCCGGCGGTTCCCCTTCCCCTTGCGTTGACGTCACTACGATGAGCAGTTTTTCGCTGGCGATTTGTTTGAATTTATAGTCGCCCGCGTTCACCAGCTTAACGTTCAGTTTTGCTGCTAATAAATCATCACGTAATGCTTCAGCA  >  NC_000913/2891409‑2891665

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑