Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,927,714	T→C	intergenic (+42/‑515)	ppnN → / → sdaC	nucleotide 5'‑monophosphate nucleosidase/serine:H(+) symporter SdaC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,927,714	0	T	C	100.0%	210.8 / NA	59	intergenic (+42/‑515)	ppnN/sdaC	nucleotide 5'‑monophosphate nucleosidase/serine:H(+) symporter SdaC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (29/30);  total (29/30)

GTATGGACGACCTGCTACAGGGTTTTGTTGCCCAGCATCGTATGAAGTTGCCAGGCTCAGCCTACATCCCTTGCTACGAAATCTGCACGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGGGTTTTCTCGTTTTTGGT‑‑CACTTACTCATCAACTCATTTCATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAA  >  NC_000913/2927581‑2927848                                                                                                                                      |                                                                                                                                         gtATGGACGACCTGCTACAGGGTTTTGTTGCCCAGCATCGTATGAAGTTGCCAGGCTCAGCCTACATCCCTTGCTACGAAATCTGCACGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGGGTTTTCTCGTCTTTGGt                                                                                                                                    <  1:129868/140‑1 (MQ=255)      gacgacCTGCTACAGGGTTTTGTTGCCCAGCATCGTATGAAGTTGCCAGGCTCAGCCTACATCCCTTGCTACGAAATCTGCACGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGGGTTTTCTCGTCTTTGGT‑‑CACtt                                                                                                                             <  1:528186/140‑1 (MQ=255)               cTACAGGGTTTTGTTGCCCAGCATCGTATGAAGTTGCCAGGCTCAGCCTACATCCCTTGCTACGAAATCTGCACGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGGGTTTTCTCGTCTTTGGTCACACTTACtcatc                                                                                                                      >  2:413893/1‑140 (MQ=255)                         ttgttgCCCAGCATCGTATGAAGTTGCCAGGCTCAGCCTACATCCCTTGCTACGAAATCTGCACGTAATCTCCGCTCCCCCGTTGACGTTTGTTGCCGGGTTTTCCCGTCTTTGGC‑‑CACTTACTCATCAACtcatttcat                                                                                                          >  2:420224/1‑140 (MQ=255)                         ttgttgCCCAGCATCGTATGAAGTTGCCAGGCTCAGCCTACATCCCTTGCTACGAAATCTGCACGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGGGTTTTCTCGTCTTTGGT‑‑CACTTACTCATCAACtcatttcat                                                                                                          >  1:102089/1‑140 (MQ=255)                            ttgCCCAGCATCGTATGAAGTTGCCAGGCTCAGCCTACATCCCTTGCTACGAAATCTGCACGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGGGTTTTCTCGTCTTTGGT‑‑CACTTACTCATCAACTCATTTCATTTg                                                                                                       <  1:420167/140‑1 (MQ=255)                               cccAGCATCGTATGAAGTTGCCAGGCTCAGCCTACATCCCTTGCTACGAAATCTGCACGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGGGTTTTCTCGTCTTTGGT‑‑CACCTACTCATCAACTCATTTCATTTGTta                                                                                                    >  2:612478/1‑140 (MQ=255)                                 cAGCATCGTATGAAGTTGCCAGGCTCAGCCTACATCCCTTGCTACGAAATCTGCACGTAATCTCCGCTCCCCCGGCGACGTTTGTTGCCGGGTTTTCTCGTCTTTGGT‑‑CACTTACTCATCAACTCATTTCATTTGTtata                                                                                                  >  1:132237/1‑140 (MQ=255)                                    cATCGTATGAAGTTGCCAGGCTCAGCCTACATCCCTTGCTACGAAATCTGCACGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGGGTTTTCTCGTCTTTGGT‑‑CACTTACTCATCAACtcatttcatt                                                                                                         >  2:359120/1‑130 (MQ=255)                                    cATCGTATGAAGTTGCCAGGCTCAGCCTACATCCCTTGCTACGAAATCTGCACGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGGGTTTTCTCGTCTTTGGT‑‑CACTTACTCATCAACtcatttcatt                                                                                                         <  1:359120/130‑1 (MQ=255)                                         tATGAAGTTGCCAGGCTCAGCCTACATCCCTTGCTACGAAATCTGCACGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGGGTTTTCTCGTCTTTGGT‑‑CACTTACTCATCAACTCATTTCATTTGTTATATGAATGtt                                                                                          >  2:566119/1‑140 (MQ=255)                                         tATGAAGTTGCCAGGCTCAGCCTACATCCCTTGCTACGAAATCTGCACGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGGGTTTTCTCGTCTTTGGT‑‑AACTTACTCATCAACTCATTTCATTTGTTATATGAATGtt                                                                                          >  2:286458/1‑140 (MQ=255)                                              aGTTGCCAGGCTCAGCCTACATCCCTTGCTACGAAATCTGCACGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGGGTTTTCTCGTCTTTGGT‑‑CACTTACTCATCAACTCATTTCATTTGTTATATGAATGTTTCTTa                                                                                     >  2:133289/1‑140 (MQ=255)                                                   ccAGGCTCAGCCTACATCCCTTGCTACGAAATCTGCGCGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGGGTTTTCTCGTCTTTGGT‑‑CACTTACTCATCAACTCATTTCATTTGTTATATGAATGTTTCTTaccacc                                                                                >  2:136739/1‑140 (MQ=255)                                                   ccAGGCTCAGCCTACATCCCTTGCTACGAAATCTGCACGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGGGTTTTCTCGTCTTTGGT‑‑CACTTACTCATCAACTCATTTCATTTGTTATATGAATGTTTCTTaccacc                                                                                >  1:349959/1‑140 (MQ=255)                                                   ccAGGCTCAGCCTACATCCCTTGCTACGAAATCTGCACGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGGGTTTTCTCGTCTTTGGT‑‑CACTTACTCATCAACTCATTTCATTTGTTATATGAATGTTTCTTaccacc                                                                                >  1:464049/1‑140 (MQ=255)                                                     aGGCTCAGCCTACATCCCTTGCTACGAAATCTGCACGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGGGTTTTCTCGTCTTTGGT‑‑CACTTACTCATCAACTCATTTCATTTGTTATATGAATGTTTCTTACCACCCt                                                                              <  2:349959/140‑1 (MQ=255)                                                       gCTCAGCCTACATCCCTTGCTACGAAATCTGCACGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGGGTTTTCTCGTCTTTGGT‑‑CACTTACTCATCAACTCATTTCATTTGTTATATGAATGTTTCTTACCACCCTCa                                                                            >  2:503480/1‑140 (MQ=255)                                                       gCTCAGCCTACATCCCTTGCTACGAAATCTGCACGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGGGTTTTCTCGTCTTTGGT‑‑CACTTACTCATCAACTCATTTCATTTGTTATATGAATGTTTCTTACCACCCTCa                                                                            <  1:391025/140‑1 (MQ=255)                                                        cTCAGCCTACATCCCTTGCTACGAAATCTGCACGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGGGTTTTCTCGTCTTTGGT‑‑CACTTACTCATCAACTCATTTCATTTGTTATATGAATGTTTCTTACCACCCTCa                                                                            >  1:448173/1‑139 (MQ=255)                                                        cTCAGCCTACATCCCTTGCTACGAAATCTGCACGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGGGTTTTCTCGTCTTTGGT‑‑CACTTACTCATCAACTCATTTCATTTGTTATATGAATGTTTCTTACCACCCTCa                                                                            <  2:448173/139‑1 (MQ=255)                                                         tCAGCCTACATCCCTTGCTACGAAATCTGCACGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGGGTTTTCTCGTCTTTGGT‑‑CACTTACTCATCAACTCATTTCATTTGTTATATGAATGTTTCTTACCACCCTCAcg                                                                          <  1:15808/140‑1 (MQ=255)                                                           aGCCTACATCCCTTGCTACGAAATCTGCACGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGGGTTTTCTCGTCTTTGGT‑‑CACTTACTCATCAACTCATTTCATTTGTTATATGAATGTTTCTTACCACCCTCAcgcg                                                                        >  2:556766/1‑140 (MQ=255)                                                              cTACATCCCTTGCTACGAAATCTGCACGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGGGTTTTCTCGTCTTTGGT‑‑CACTTACTCATCAACTCATTTCATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACa                                                                     <  2:501504/140‑1 (MQ=255)                                                              cTACATCCCTTGCTACGAAATCTGCACGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGGGTTTTCTCGTCTTTGGT‑‑CACTTACTCATCAACTCATTTCATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACa                                                                     <  2:58336/140‑1 (MQ=255)                                                                 cATCCCTTGCTACGAAATCTGCACGTAATCTCCGCTCCCGCGGTGACGTTTGTTGCCGGGTTTTCTCGTCTTTGGT‑‑CACTTACTCATCAACTCATTTCATTTGTTATATGAATGTTTCTTACCACCCTCAGGCGACAAat                                                                  >  2:387791/1‑140 (MQ=255)                                                                     ccTTGCTACGAAATCTGCACGTAATCTCCGCTCCCCCGGTTACGTTTGTTGCCGGGTTTTCTCGTCTTTGGT‑‑CACTTACTCATCAACTCATTTCATTTGTTATATGAATGTTTCTTACCACGCTCACGCGACAAATatca                                                              >  2:478260/1‑140 (MQ=255)                                                                     ccTTGCTACGAAATCTGCACGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGGGTTTTCTCGTCTTTGGT‑‑CACTTACTCATCAACTCATTTCATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATatca                                                              <  1:141866/140‑1 (MQ=255)                                                                       ttGCTACGAAATCTGCACGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGGGTTTTCTCGTCTTTGGT‑‑CACTTACTCATCAACTCATTTGATTTGTTAGATGAAGGATTCTTACCTCCCTAGCGCGACAAATatcatc                                                            >  2:317120/1‑140 (MQ=255)                                                                       ttGCTACGAAATCTGCACGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGGGTTTTCTCGTCTTTGGT‑‑CACTTACTCATCAACTCATTTCATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATatcatc                                                            >  2:608779/1‑140 (MQ=255)                                                                       ttGCTACGAAATCTGCACGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGGGTTTTCTCGTCTTTGGT‑‑CACTTACTCATCAACTCATTTCATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATatcatc                                                            >  1:466147/1‑140 (MQ=255)                                                                       ttGCTACGAAATCTGCACGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGGGTTTTCTCGTCTTTGGT‑‑CACTTACTCATCAACTCATTTCATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATatcatc                                                            >  2:566296/1‑140 (MQ=255)                                                                        tGCTACGAAATCTGCACGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGGGTTTTCTCGTCTTTGGT‑‑CACTTACTCATCAACTCATTTCATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATAtcatca                                                           >  2:458216/1‑140 (MQ=255)                                                                        tGCTACGAAATCTGCACGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGGGTTTTCTCGTCTTTGGT‑‑CACTTACTCATCAACTCATTTCATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATAtcatca                                                           <  2:155715/140‑1 (MQ=255)                                                                        tGCTACGAAATCTGCACGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGGGTTTTCTCGTCTTTGGT‑‑CACTTACTCATCAACTCATTTCATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATAtcatca                                                           <  2:575290/140‑1 (MQ=255)                                                                         gCTACGAAATCTGCACGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGGGTTTTCTCGTCTTTGGT‑‑CACTTACTCATCAACTCATTTCATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATcac                                                          <  1:359192/140‑1 (MQ=255)                                                                         gCTACGAAATCTGCACGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGGGTTTTCTCGTCTTTGGT‑‑CACTTACTCATCAACTCATTTCATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATcac                                                          <  1:503480/140‑1 (MQ=255)                                                                                aaTCTGCACGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGGGTTTTCTCGTCTTTGGT‑‑CACTTACTCATCAACTCATTTCATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTaa                                                    <  2:584078/139‑1 (MQ=255)                                                                                aaTCTGCACGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGGGTTTTCTCGTCTTTGGT‑‑CACTTACTCATCAACTCATTTCATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTaa                                                    >  1:584078/1‑139 (MQ=255)                                                                                aaTCTGCACGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGGGTTTTCTCGTCTTTGGT‑‑CACTTACTCATCAACTCATTTCATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAat                                                   <  2:566195/140‑1 (MQ=255)                                                                                aaTCTGCACGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGGGTTTTCTCGTCTTTGGT‑‑CACTTACTCATCAACTCATTTCATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAat                                                   <  1:552241/140‑1 (MQ=255)                                                                                aaTCTGCACGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGGGTTTTCTCGTCTTTGGT‑‑CACTTACTCATCAACTCATTTCATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAat                                                   <  1:566119/140‑1 (MQ=255)                                                                                aaTCTGCACGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGGGTTTTCTCGTCTTTGGT‑‑CACTTACTCATCAACTCATTTCATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAat                                                   <  2:466147/140‑1 (MQ=255)                                                                                   cTGCACGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGGGTTTTCTCGTCTTTGGT‑‑CACTTACTCATCAACTCATTTCATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATg                                                <  2:30245/140‑1 (MQ=255)                                                                                       gcGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGGGTTTTCTCGTCTTTGGT‑‑CACTTACTCATCAACTCATTTCATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCAt                                            <  1:136739/139‑1 (MQ=255)                                                                                       aCGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGGGTTTTCTCGTCTTTGGT‑‑CACTTACTCATCAACTCATTTCATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCAt                                            >  2:491515/1‑140 (MQ=255)                                                                                          tAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGGGTTTTCTCGTCTTTGGT‑‑CACTTACTCATCAACTCATTTCATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACa                                         <  2:458003/140‑1 (MQ=255)                                                                                                   cTCCCCCGGTGACGTTTGTTGCCGGGTTTTCTCGTCTTTGGT‑‑CACTTACTCATCAACTCATTTCATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGtt                                >  1:524971/1‑140 (MQ=255)                                                                                                    tCCCCCGGTGACGTTTGTTGCCGGGTTTTCTCGTCTTTGGT‑‑CACTTACTCATCAACTCATTTCATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTg                               <  1:491515/140‑1 (MQ=255)                                                                                                      ccccGGTGACGTTTGTTGCCGGGTTTTCTCGTCTTTGGTCACACTTACTCATCAACTCATTTCATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTg                               <  1:413893/140‑1 (MQ=255)                                                                                                         cGGTGACGTTTGTTGCCGGGTTTTCTCGTCTTTGGT‑‑CACTTACTCATCAACTCATTTCATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACg                          >  1:79805/1‑140 (MQ=255)                                                                                                           gTGACGTTTGTTGCCGGGTTTTCTCGTCTTTGGT‑‑CACTTACTCATCAACTCATTTCATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCa                        <  1:133289/140‑1 (MQ=255)                                                                                                                  ttgttgCCGGGTTTTCTCGTCTTTGGT‑‑CACTTACTCATCAACTCATTTCATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGttt                 >  2:111160/1‑140 (MQ=255)                                                                                                                     ttgCCGGGTTTTCTCGTCTTTGGT‑‑CACTTACTCATCAACTCATTTCATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTccc              >  1:607695/1‑140 (MQ=255)                                                                                                                      tgCCGGGTTTTCTCGTCTTTGGT‑‑CACTTACTCATCAACTCATTTCATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCt             >  2:356766/1‑140 (MQ=255)                                                                                                                       gCCGGGTTTTCTCGTCTTTGGT‑‑CACTTACTCATCAACTCATTTCATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTa            <  2:464049/140‑1 (MQ=255)                                                                                                                           ggTTTTCTCGTCTTTGGT‑‑CACTTACTCATCAACTCATTTCATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTAtttt        >  1:127966/1‑140 (MQ=255)                                                                                                                              tttCTCGTCTTTGGT‑‑CACTTACTCATCAACTCATTTCATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCAt     <  2:79805/140‑1 (MQ=255)                                                                                                                               ttCTCGTCTTTGGT‑‑CACTTACTCATCAACTCATTTCATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATa    <  1:164254/140‑1 (MQ=255)                                                                                                                                 ctcGTCTTTGGT‑‑CACTTACTCATCAACTCATTTCATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATaaa  <  1:478260/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                         GTATGGACGACCTGCTACAGGGTTTTGTTGCCCAGCATCGTATGAAGTTGCCAGGCTCAGCCTACATCCCTTGCTACGAAATCTGCACGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGGGTTTTCTCGTTTTTGGT‑‑CACTTACTCATCAACTCATTTCATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAA  >  NC_000913/2927581‑2927848

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑