Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,937,272 T→C R563R (CGT→CGC)  fucI → L‑fucose isomerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,937,2720TC95.1% 129.4 / ‑2.0 41R563R (CGT→CGC) fucIL‑fucose isomerase
Reads supporting (aligned to +/- strand):  ref base T (1/1);  new base C (17/22);  total (18/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.78e-01

ACTCGGTAATGGCGAACTGGGGCGCTAACCATGGGGTTCTGACCATCGGCCACGTTGGCGCAGACTTTATCACTCTCGCCTCCATGCTGCGTATCCCGGTATGTATGCACAACGTTGAAGAGACCAAAGTGTATCGTCCTTCTGCCTGGGCTGCGCACGGCATGGATATTGAAGGCCAGGATTACCGCGCTTGCCAGAACTACGGTCCGTTGTACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGTCGCTCCGGCTACCGGGCCT  >  NC_000913/2937136‑2937406
                                                                                                                                        |                                                                                                                                      
aCTCGGTAATGGCGAACTGGGGTGCTAACCATGCGCTTCCGACCATCGGCCACGTTGGCGCAGACTTTATCGCTCTCGCCTCCATGCTGCGTATCCCGGTATGTATGCACAACGTTGAAGAGACCAAAGTGTATCGCCCt                                                                                                                                     <  2:294795/140‑1 (MQ=255)
                 tGGGGCGCTAACCATGGGGTTCTGACCATCGGCCACGTTGGCGCAGACTTTATCACTCTCGCCTCCATGCTGCGTATCCCGGTATGTATGCACAACGTTGAAGAGACCAAAGTGTATCGCCCTTCTGCCTGGGCTGCGCa                                                                                                                    <  1:398781/140‑1 (MQ=255)
                     gcgcTAACCATGGGGTTCTGACCATCGGCCACGTTGGCGCAGACTTTATCACTCTCGCCTCCATGCTGCGTATCCCGGTATGTATGCACAACGTTGAAGAGACCAAAGTGTATCGCCCTTCTGCCTGGGCTGCGCACGGc                                                                                                                >  2:41232/1‑140 (MQ=255)
                      cgcTAACCATGGGGTTCTGACCATCGGCCACGTTGGCGCAGACTTTATCACTCTCGCCTCCATGCTGCGTATCCCGGTATGTATGCACAACGTTGAAGAGACCAAAGTGTATCGCCCTTCTGCCTGGGCTGCGCACGGCa                                                                                                               <  1:397048/140‑1 (MQ=255)
                       gcTAACCATGGGGTTCTGACCATCGGCCACGTTGGCGCAGACTTTATCACTCTCGCCTCCATGCTGCGTATCCCGGTATGTATGCACAACGTTGAAGAGACCAAAGTGTATCGCCCTTCTGCCTGGGCTGCGCACGGCAt                                                                                                              <  1:637567/140‑1 (MQ=255)
                                     tCTGACCATCGGCCACGTTGGCGCAGACTTTATCACTCTCGCCTCCATGCTGCGTATCCCGGTATGTATGCACAACGTTGAAGAGACCAAAGTGTATCGCCCTTCTGCCTGGGCTGCGCACGGCATGGATATTGAAGGcc                                                                                                >  1:136482/1‑140 (MQ=255)
                                       tGACCATCGGCCACGTTGGCGCAGACTTTATCACTCTCGCCTCCATGCTGCGTATCCCGGTATGTATGCACAACGTTGAAGAGACCAAAGTGTATCGCCCTTCTGCCTGGGCTGCGCACGGCATGGATATTGAAGGCCAg                                                                                              >  2:62468/1‑140 (MQ=255)
                                            aTCGGCCACGTTGGCGCAGACTTTATCACTCTCGCCTCCATGCTGCGTATCCCGGTATGTATGCACAACGTTGAAGAGACCAAAGTGTATCGCCCTTCTGCCTGGGCTGCGCACGGCATGGATATTGAAGGCCAGGATTa                                                                                         >  1:487475/1‑140 (MQ=255)
                                              cGGCCACGTTGGCGCAGACTTTATCACTCTCGCCTCCATGCTGCGTATCCCGGTATGTATGCACAACGTTGAAGAGACCAAAGTGTATCGCCCTTCTGCCTGGGCTGCGCACGGCATGGATATTGAAGGCCAGGATTAcc                                                                                       >  2:342935/1‑140 (MQ=255)
                                                gCCACGTTGGCGCAGACTTTATCACTCTCGCCTCCATGCTGCGTATCCCGGTATGTATGCACAACGTTGAAGAGACCAAAGTGTATCGCCCTTCTGCCTGGGCTGCGCACGGCATGGATATTGAAGGCCAGGATTACcgc                                                                                     >  1:647734/1‑140 (MQ=255)
                                                 ccACGTTGGCGCAGACTTTATCACTCTCGCCTCCATGCTGCGTATCCCGGTATGTATGCACAACGTTGAAGAGACCAAAGTGTATCGCCCTTCTGCCTGGGCTGCGCACGGCATGGATATTGAAGGCCAGGATTACcgcg                                                                                    <  2:496314/140‑1 (MQ=255)
                                                 ccACGTTGGCGCAGACTTTATCACTCTCGCCTCCATGCTGCGTATCCCGGTATGTATGCACAACGTTGAAGAGACCAAAGTGTATCGCCCTTCTGCCTGGGCTGCGCACGGCATGGATATTGAAGGCCAGGATTACcgcg                                                                                    >  2:151739/1‑140 (MQ=255)
                                                          cgcAGACTTTATCACCCTCGCCACCGTGCAGCGTATCTCGGTATGTATGCACAACGTTGAAGAGACCAAAGTGTATCGCCCTTCTGCCTGGGCTGCGCACGGCATGGATATTGAAGGCCAGGATTACCGCGCTTGCCAGa                                                                           <  1:205265/140‑1 (MQ=255)
                                                               aCTTTATCACTCTCGCCTCCATGCTGCGTATCCCGGTATGTATGCACAACGTTGAAGAGACCAAAGTGTATCGCCCTTCTGCCTGGGCTGCGCACGGCATGGATATTGAAGGCCAGGATTACCGCGCTTGCCAGAACTAc                                                                      >  1:597297/1‑140 (MQ=255)
                                                                     tCACTCTCGCCTCCATGGTGCGTATCCCGGTTTGTGTGCCCAACGTTGAAGAGACCAAAGTGTATCGCCCTTCTGCCTGGGCTGCGCACGGCATGGATATTGAAGGCCAGGATTACCGCGCTTGCCAGAACTACGGTCCg                                                                <  2:202057/140‑1 (MQ=255)
                                                                      cACTCTCGCCTCCATGCTGCGTATCCCGGTATGTATGCACAACGTTGAAGAGACCAAAGTGTATCGCCCTTCTGCCTGGGCTGCGCACGGCATGGATATTGAAGGCCAGGATTACCGCGCTTGCCAGAACTACGGTCCGt                                                               <  1:560594/140‑1 (MQ=255)
                                                                          ctcGCCTCCATGCTGCGAATCCCGGTATGTATGCACAACGTTGAAGAGGCCAAAGTGTATCGCCCTTCTGCCTGGGCTGCGCACGGCATGGATATTGAAGGCCAGGATTACCGCGCTTGCCAGAACTACGGTCCGTTGTa                                                           <  2:272570/140‑1 (MQ=255)
                                                                           tcGCCTCCATGCTGCGTATCCCGGTATGTATGCACAACGTTGAAGAGACCAAAGTGTATCGTCCTTCTGCCTGGGCTGCGCACGGCATGGATATTGAAGGCCAGGATTACCGCGCTTGCCAGAACTACGGTCCGTTGTAc                                                          >  2:22383/1‑140 (MQ=255)
                                                                           tcGCCTCCATGCTGCGTATCCCGGTATGTATGCACAACGTTGAAGAGACCAAAGTGTATCGCCCTTCTGCCTGGGCTGCGCACGGCATGGATATTGAAGGCCAGGATTACCGCGCTTGCCAGAACTACGGTCCGTTGTAc                                                          >  1:408282/1‑140 (MQ=255)
                                                                            cGCCTCCATGCTGCGTATCCCGGTATGTATGCACAACGTTGAAGAGACCAAAGTGTATCGCCCTTCTGCCTGGGCTGCGCACGGCATGGATATTGAAGGCCAGGATTACCGCGCTTGCCAGAACTACGGTCCGTTGTACa                                                         <  2:340443/140‑1 (MQ=255)
                                                                                 ccATGCTGCGTATCCCGGTATGTATGCACAACGTTGAAGAGACCAAAGTGTATCGCCCTTCTGCCTGGGCTGCGCACGGCATGGATATTGAAGGCCAGGATTACCGCGCTTGCCAGAACTACGGTCCGTTGTACAAGCGt                                                    <  2:146727/140‑1 (MQ=255)
                                                                                  cATGCTGCGTATCCCGGTATGTATGCACAACGTTGAAGAGACCAAAGTGTATCGCCCTTCTGCCTGGGCTGCGCACGGCATGGATATTGAAGGCCAGGATTACCGCGCTTGCCAGAATTACGGCCCGTTGTACAAGCGtt                                                   >  2:296098/1‑140 (MQ=255)
                                                                                   aTGCTGCGTATCCCGGTATGTATGCACAACGTTGAAGAGACCAAAGTGTATCGCCCTTCTGCCTGGGCTGCGCACGGCATGGATATTGAAGGCCAGGATTACCGCGCTTGCCAGAACTACGGTCCGTTGTACAAGCGTTa                                                  <  1:342935/140‑1 (MQ=255)
                                                                                           tATCCCGGTATGTATGCACAACGTTGAAGAGACCAAAGTGTATCGCCCTTCTGCCTGGGCTGCGCACGGCATGGATATTGAAGGCCAGGATTACCGCGCTTGCCAGAACTACGGTCCGTTGTACAAGCGTTAATACATTa                                          <  2:487475/140‑1 (MQ=255)
                                                                                             tCCCGGTATGTATGCACAACGTTGAAGAGACCAAAGTGTATCGCCCTTCTGCCTGGGCTGCGCACGGCATGGATATTGAAGGCCAGGATTACCGCGCTTGCCAGAACTACGGTCCGTTGTACAAGCGTTAATACATTACt                                        <  2:493219/140‑1 (MQ=255)
                                                                                                          gCACAACGTTGAAGAGACCAAAGTGTATCGCCCTTCTGCCTGGGCTGCGCACGGCATGGATATTGAAGGCCAGGATTACCGCGCTTGCCAGAACTACGGTCCGTTGTACAAGCGTTAATACATTACTCCCtgatgtgatg                           <  2:597297/140‑1 (MQ=255)
                                                                                                               aCGTTGAAGAGACCAAAGTGTATCGCCCTTCTGCCTGGGCTGCGCACGGCATGGATATTGAAGGCCAGGATTACCGCGCTTGCCAGAACTACGGTCCGTTGTACAAGCGTTAATACATTACTCCCTGATGTGATGCCCgg                      <  1:62468/140‑1 (MQ=255)
                                                                                                               aCGTTGAAGAGACCAAAGTGTATCGCCCTTCTGCCTGGGCTGCGCACGGCATGGATATTGAAGGCCAGGATTACCGCGCTTGCCAGAACTACGGTCCGTTGTACAAGCGTTAATACATTACTCCCTGATGTGATGCCCgg                      <  1:41232/140‑1 (MQ=255)
                                                                                                                cGTTGAAGAGACCAAAGTGTATCGCCCTTCTGCCTGGGCTGCGCACGGCATGGATATTGAAGGCCAGGATTACCGCGCTTGCCAGAACTACCGTCCGTTGGACATGCGTTAATACATGACTCACTGATGTGATGCCCGGt                     >  2:279281/1‑140 (MQ=255)
                                                                                                                  ttGAAGAGACCAAAGTGTATCGCCCTTCTGCCTGGGCTGCGCACGGCATGGATATTGAAGGCCAGGATTACCGCGCTTGCCAGAACTACGGTCCGTTGTACATGCGTTAAGACAGTAGTCCCTGATGTGATGCCCGGTCg                   >  1:177427/1‑140 (MQ=255)
                                                                                                                  ttGAAGAGACCAAAGTGTATCGCCCTTCTGCCTGGGCTGCGCACGGCATGGATATTGAAGGCCAGGATTACCGCGCTTGCCAGAACTACGGTCCGTTGTACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGTCg                   <  2:408282/140‑1 (MQ=255)
                                                                                                                   tGAAGAGACCAAAGTGTTTCGCACTTCTGCCTGGGCTGCCCACGGCATTGATATTGAAGGCCAGGATTACCGCGCTTGCCAGAACTACGGTCCGTTGTACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGTCGc                  <  2:177427/140‑1 (MQ=255)
                                                                                                                   tGAAGAGACCAAAGTGTATCGCCCTTCTGCCTGGGCTGCGCACGGCATGGATATTGAAGGCCAGGATTACCGCGCTTGCCAGAACTACGGTCCGTTGTACAAGCGTTAATACATTACTCCCTGATGTGATGTCCGGTCGc                  >  2:479556/1‑140 (MQ=255)
                                                                                                                    gAAGAGACCAAAGTGTATCGTCCTTCTGCCTGGGCTGCGCACGGCATGGATATTGAAGGCCAGGATTACCGCGCTTGCCAGAACTACGGTCCGTTGTACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGTCGCt                 <  1:22383/140‑1 (MQ=255)
                                                                                                                    gAAGAGACCAAAGTGTATCGCCCTTCTGCCTGGGCTGCGCACGGCATGGATATTGAAGGCCAGGATTACCGCGCTTGCCAGAACTACGGTCCGTTGTACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGTCGCt                 <  2:136482/140‑1 (MQ=255)
                                                                                                                          aCCAAAGTGTATCGCCCTTCTGCCTGGGCTGCGCACGGCATGGATATTGAAGGCCAGGATTACCGCGCTTGCCAGAACTACGGTCCGTTGTACAAGCGTTTATAAATTACTCCCTGATGTGAAGCCCGGTCGCTCCGGCt           >  2:220823/1‑140 (MQ=255)
                                                                                                                          aCCAAAGTGTATCGCCCTTCTGCCTGGGCTGCGCACGGCATGGATATTGAAGGCCAGGATTACCGCGCTTGCCAGAACTACGGTCCGTTGTACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGTCGCTCCGGCt           >  2:390336/1‑140 (MQ=255)
                                                                                                                          aCCAAAGTGTATCGCCCTTCTGCCTGGGCTGCGCACGGCATGGATATTGAAGGCCAGGATTACCGCGCTTGCCAGAACTACGGTCCGTTGTACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGTCGCTCCGGCt           >  2:333699/1‑140 (MQ=255)
                                                                                                                            cAAAGTGTATCGCCCTTCTGCCTGGGCTGCGCACGGCATGGATATTGAAGGCCAGGATTACCGCGCTTGCCAGAACTACGGTCCGTTGTACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGTCGCTCCGGCTAc         <  1:333699/140‑1 (MQ=255)
                                                                                                                            cAAAGTGTATCGCCCGTCTGCCTGGTCTGCGCACGGCATGGATATTGAAGGCCAGGATTACCGCGCTTGCCAGAACTACGGTCCGTTGTACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGTCGCTCCGGCTAc         <  1:279281/140‑1 (MQ=255)
                                                                                                                               aGTGTATCGCCCTTCTGCCTGGGCTGCGCACGGCATGGATATTGAAGGCCAGGATTACCGCGCTTGCCAGAACTACGGTCCGTTGTACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGTCGCTCCGGCTACCgg      >  2:408345/1‑140 (MQ=255)
                                                                                                                                   tATCGCCCTTCTGCCTGGGCTGCGCACGGCATGGATATTGAAGGCCAGGATTACCGCGCTTGCCAGAACTACGGTCCGTTGTACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGTCGCTCCGGCTACCGGGCCt  <  1:151739/140‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                      
ACTCGGTAATGGCGAACTGGGGCGCTAACCATGGGGTTCTGACCATCGGCCACGTTGGCGCAGACTTTATCACTCTCGCCTCCATGCTGCGTATCCCGGTATGTATGCACAACGTTGAAGAGACCAAAGTGTATCGTCCTTCTGCCTGGGCTGCGCACGGCATGGATATTGAAGGCCAGGATTACCGCGCTTGCCAGAACTACGGTCCGTTGTACAAGCGTTAATACATTACTCCCTGATGTGATGCCCGGTCGCTCCGGCTACCGGGCCT  >  NC_000913/2937136‑2937406

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑