Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,004,053	A→G	A578A (GCT→GCC)	ygeV ←	putative sigma(54)‑dependent transcriptional regulator YgeV

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,004,053	0	A	G	100.0%	192.0 / NA	54	A578A (GCT→GCC)	ygeV	putative sigma(54)‑dependent transcriptional regulator YgeV
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (37/17);  total (37/17)

GATTGTAAATACAGTTCTGGATTGCGAGAAAACGAAGTAAAAGGATATCCGGCCTGAATTCAGGCCGGATTCACTGAGGTTATGTGTTTAACAACTCATATTTCTTAATCTTGCGATAGAGCGTAGCAATGCCGATGCCCAGTTCATCAGCAACTTGCTTCTTGCTGTTATGACGTGAAAGCGCCTCGCGGATCATTTGCTTTTCCATCTCCTCCAGCGCCGTGCCGCCCGCATCATCGAGTGACAGGTGCGCCTCACTGA  >  NC_000913/3003929‑3004189                                                                                                                             |                                                                                                                                         gatTGTAAATACAGTTCTGGATTGCGAGAAAACGAAGTAAAAGGATATCCGGCCTGAATTCAGGCCGGATTCACTGAGGTTATGTGTTTAACAACTCATATTTCTTAATCTTGCGATAGAGCGTGGCAATGCCGATGccc                                                                                                                           >  2:386242/1‑140 (MQ=255) gatTGTAAATACAGTTCTGGATTGCGAGAAAACGAAGTAAAAGGATATCCGGCCTGAATTCAGGCCGGATTCACTGAGGTTATGTGTTTAACAACTCATATTTCTTAATCTTGCGATAGAGCGTGGCAATGCCGATGccc                                                                                                                           >  1:161537/1‑140 (MQ=255) gatTGTAAATACAGTTCTGGATTGCGAGAAAACGAAGTAAAAGGATATCCGGCCTGAATTCAGGCCGGATTCACTGAGGTTATGTGTTTAACAACTCATATTTCTTAATCTTGCGATAGAGCGTGGCAATGCCGATGccc                                                                                                                           >  2:566622/1‑140 (MQ=255) gatTGTAAATACAGTTCTGGATTGCGAGAAAACGAAGTAAAAGGATATCCGGCCTGAATTCAGGCCGGATTCACTGAGGTTATGTGTTTAACAACTCATATTTCTTAATCTTGCGATAGAGCGTGGCAATGCCGATGccc                                                                                                                           >  1:355350/1‑140 (MQ=255)   ttGTAAATACAGTTCTGGATTGCGAGAAAACGAAGTAAAAGGATATCCGGCCTGAATTCAGGCCGGATTCACTGAGGTTATGTGTTTAACAACTCATATTTCTTAATCTTGCGATAGAGCGTGGCAATGCCGATGCCCAg                                                                                                                         >  1:85951/1‑140 (MQ=255)          tACAGTTCTGGATTGCGAGAAAACGAAGTAAAAGGATATCCGGCCTGAATTCAGGCCGGATTCACTGAGGTTATGTGTTTAACAACTCATATTTCTTAATCTTGCGATAGAGCGTGGCAATGCCGATGCCCAGTtcatca                                                                                                                  <  1:460571/140‑1 (MQ=255)             aGTTCTGGATTGCGAGAAAACGAAGTAAAAGGATATCCGGCCTGAATTCAGGCCGGATTCACTGAGGTTATGTGTTTAACAACTCATATTTCTTAATCTTGCAATAGAGCGTGGAAAAGCCGATGCCCAGTTCATCAGCa                                                                                                               >  1:229096/1‑140 (MQ=255)              gTTCTGGATTGCGAGAAAACGAAGTAAAAGGATATCCGGCCTGAATTCAGGCCGGATTCACTGAGGTTATGTGTTTAACAACTCATATTTCTTAATCTTGCGATAGAGCGTGGCGATGCCGATGACCAGTTCATCAGCaa                                                                                                              >  1:315401/1‑140 (MQ=255)              gTTCTGGATTGCGAGAAAACGAAGTAAAAGGATATCCGGCCTGAATTCAGGCCGGATTCACTGAGGTTATGTGTTTAACAACTCATATTTCTTAATCTTGCGATAGAGCGTGGCAATGCCGATGCCCAGTTCATCAGCaa                                                                                                              >  1:54889/1‑140 (MQ=255)              gTTCTGGATTGCGAGAAAACGAAGTAAAAGGATATCCGGCCTGAATTCAGGCCGGATTCACTGAGGTTATGTGTTTAACAACTCATATTTCTTAATCTTGCGATAGAGCGTGGCAATGCCGATGCCCAGTTCATCAGCaa                                                                                                              >  1:425000/1‑140 (MQ=255)                tCTGGATTGCGAGAAAACGAAGTAAAAGGATATCCGGCCTGAATTCAGGCCGGATTCACTGAGGTTATGTGTTTAACAACTCATATTTCTTAATCTTGCGATAGAGCGTGGCAATGCCGATGcc                                                                                                                            >  1:355864/1‑124 (MQ=255)                tCTGGATTGCGAGAAAACGAAGTAAAAGGATATCCGGCCTGAATTCAGGCCGGATTCACTGAGGTTATGTGTTTAACAACTCATATTTCTTAATCTTGCGATAGAGCGTGGCAATGCCGATGcc                                                                                                                            <  2:355864/124‑1 (MQ=255)                tCTGGATTGCGAGAAAACGAAGTAAAAGGATATCCGGCCTGAATTCAGGCCGGATTCACTGAGGTTATGTGTTTAACAACTCATATTTCTTAATCTTGCGATAGAGCGTGGCAATGCCGATGCCCAGTTCATCAGCAACt                                                                                                            >  1:502485/1‑140 (MQ=255)                tCTGGATTGCGAGAAAACGAAGTAAAAGGATATCCGGCCTGAATTCAGGCCGGATTCACTGAGGTTATGTGTTTAACAACTCATATTTCTTAATCATGCGACAGAGAGTGGCAATGCCGATGTCGAGTTCATCAGCAACt                                                                                                            >  2:470944/1‑140 (MQ=255)                  tGGATTGCGAGAAAACGAAGTAAAAGGATATCCGGCCTGAATTCAGGCCGGATTCACTGAGGTTATGTGTTTAACAACTCATATTTCTTAATCTTGCGATAGCGCGTGGCAATGGCGATGCCCAGTTCATCAGCAACTTg                                                                                                          >  1:269554/1‑140 (MQ=255)                  tGGATTGCGAGAAAACGAAGTAAAAGGATATCCGGCCTGAATTCAGGCCGGATTCACTGAGGTTATGTGTTTAACAACTCATATTTCTTAATCTTGCGATAGAGCGTGGCAATGCCGATGCCCAGTTCATCAGCAACTTg                                                                                                          <  1:386242/140‑1 (MQ=255)                    gATTGCGAGAAAACGAAGTAAAAGGATATCCGGCCTGAATTCAGGCCGGATTCACTGAGGTTATGTGTTTAACAACTCATATTTCTTAATCTTGCGATAGAGCGTGGCAATGCCGATGCCCAGTTCATCAGCAACTTGct                                                                                                        >  1:589262/1‑140 (MQ=255)                       tGCGAGAAAACGAAGTAAAAGGATATCCGGCCTGAATTCAGGCCGGATTCACTGAGGTTATGTGTTTAACAACTCATATTTCTTAATCTTGCGATAGAGCGTGGCAATGCCGATGCCCAGTTCATCAGCAACTTGcttct                                                                                                     <  1:566622/140‑1 (MQ=255)                         cGAGAAAACGAAGTAAAAGGATATCCGGCCTGAATTCAGGCCGGATTCACTGAGGTTATGTGTTTAACAACTCATATTTCTTAATCTTGCGATAGAGCGTGGCAATGCCGATGCCCAGTTCATCAGCAACTTGCTTCTTg                                                                                                   >  1:338643/1‑140 (MQ=255)                         cGAGAAAACGAAGTAAAAGGATATCCGGCCTGAATTCAGGCCGGATTCACTGAGGTTATGTGTTTAACAACTCATATTTCTTAATCTTGCGATAGAGCGTGGCAATGCCGATGCCCAGCTCATCAGCAACTTGCTTCTTg                                                                                                   >  2:625411/1‑140 (MQ=255)                                  gAAGTAAAAGGATATCCGGCCTGAATTCAGGCCGGATTCACTGAGGTTATGTGTTTAACAACTCATATTTCTTAATCTTGCGATAGAGCGTGGCAATGCCGATGCCCAGTTCATCAGCAACTTGCTTCTTGCTGTTATGa                                                                                          <  2:85951/140‑1 (MQ=255)                                  gAAGTAAAAGGATATCCGGCCTCAATTCAGGGCGGATTCACTGAGGTTGTGTGTTTAACAACTCATATTTCTTAATCTTGCGATAGAGCGTGGCAATGCCGATGCCCAGTTCATCAGCAACTTGCTTCTTGCTGTTATGa                                                                                          <  2:269554/140‑1 (MQ=255)                                          aGGATATCCGGCCTGAATTCAGGCCGGATTCACTGAGGTTATGTGTTTAACAACTCATATTTCTTAATCTTGCGATAGAGCGTGGCAATGCCGATGCCCAGTTCATCAGCAACTTGCTTCTTGCTGTTATGACGTGAAAg                                                                                  <  2:161537/140‑1 (MQ=255)                                               atCCGGCCTGAATTCAGGCCGGATTCACTGAGGTTATGTGTTTAACAACTCATATTTCTTAATCTTGCGATAGAGCGTGGCAATGCCGATGCCCAGTTCATCAGCAACTTGCTTCTTGCTGTTATGACGTGAAAGCGCCt                                                                             <  2:338643/140‑1 (MQ=255)                                                tCCGGCCTGAATTCAGGCCGGATTCACTGAGGTTATGTGTTTAACAACTCATATTTCTTAATCTTGCGATAGAGCGTGGCAATGCCGATACCCAGTTCATCAGCAACTTGCTTCTTGCTGTTATGACGTGAAAGCGCCTc                                                                            >  2:566998/1‑140 (MQ=255)                                                   ggCCTGAATTCAGGCCGGATTCACTGAGGTTATGTGTTTAACAACTCATATTTCTTAATCTTGCGATAGAGCGTGGCAATGCCGATGCCCAGTTCATCAGCAACTTGCTTCTTGCTGTTATGACGTGAAAGCACCTcgcg                                                                         <  2:425000/140‑1 (MQ=255)                                                     ccTGAATTCAGGCCGGATTCACTGAGGTTATGTGTTTAACAACTCATATTTCTTAATCTTGCGATAGAGCGTGGCAATGCCGATGCCCAGTTCATCAGCAACTTGCTTCTTGCTGTTATGACGTGAAAGCGCCTCGCGGa                                                                       >  1:401433/1‑140 (MQ=255)                                                       tGAATTCAGGCCGGATTCACTGAGGTTATGTGTTTAACAACTCATATTTCTTAATCTTGCGATAGAGCGTGGCAATGCCGATGCCCAGTTCATCAGCAACTTGCTTCTTGCTGTTATGACGTGAAAGCGCCTCGCGGATc                                                                     <  1:505146/140‑1 (MQ=255)                                                               ggCCGGATTCACTGAGGTTATGTGTTTAACAACTCATATTTCTTAATCTTGCGATAGAGCGTGGCAATGCCGATGCCCAGTTCATCAGCAACTTGCTTCTTGCTGTTATGACGTGAAAGCGCCTCGCGGATCATTTGCtt                                                             >  2:560070/1‑140 (MQ=255)                                                                      ttCACTGAGGTTATGTGTTTAACAACTCATATTTCTTAATCTTGCGATAGAGCGTGGCAATGCCGATGCCCAGTTCATCAGCAACTTGCTTCTTGCTGTTATGACGTGAAAGCGCCTCGCGGATCATTTGCTTTTCCAtc                                                      <  2:589262/140‑1 (MQ=255)                                                                      ttCACTGAGGTTATGTGTTTAACAACTCATATTTCTTAATCTTGCGATAGAGCGTGGCAATGCCGATGCCCAGTTCATCAGCAACTTGCTTCTTGCTGTTATGACGTGAAAGCGCCTCGCGGATCATTTGCTTTTCCAtc                                                      <  2:389706/140‑1 (MQ=255)                                                                                  aTGTGTTTAACAACTCATATTTCTTAATCTTGCGATAGAGCGTGGCAATGCCGATGCCCAGTTCATCAGCAACTTGCTTCTTGCTGTTATGACGTGAAAGCGCCACTCGGATCATTTGCTTTGACATCTCCTCCAGCGcc                                          >  2:296780/1‑140 (MQ=255)                                                                                   tgtgTTTAACAACTCATATTTCTTAATCTTGCGATAGAGCGTGGCAATGCCGATGCCCAGTTCATCAGCAACTTGCTTCTTGCTGTTATGACGTGAAAGCGCCTCGCGGAACATATGCTTTTCCATCTCCTCCAGCGCCg                                         >  1:272256/1‑140 (MQ=255)                                                                                    gtgtTTAACAACTCATATTTCTTAATCTTGCGATAGAGCGTGGCAATGCCGATGCCCAGTTCATCAGCAACTTGCTTCTTGCTGTTATGACGTGAAAGCGCCTCGCGGATCATTTGCTTTTCCATCTCCTCCAGCGCCGt                                        >  1:100648/1‑140 (MQ=255)                                                                                       tttAACAACTCATATTTCTTAATCTTGCGATAGAGCGTGGCAATGCCGATGCCCAGTTCATCAGCAACTTGCTTCTTGCTGTTATGACGTGCAAGCGCCTGGCGAATCTTTTGCTTTGCCATATCCTCCAGCGCCGTgcc                                     >  2:207077/1‑140 (MQ=255)                                                                                       tttAACAACTCATATTTCTTAATCTTGCGATAGAGCGTGGCAATGCCGATGCCCAGTTCATCAGCAACTTGCTTCTTGCTGTTATGACGTGAAAGCGCCTCGCGGATCATTTGCTTTTCCATCTCCTCCAGCGCCGTgcc                                     >  1:40604/1‑140 (MQ=255)                                                                                       tttAACAACTCATATTTCTTAATCTTGCGATAGAGCGTGGCAATGCCGATGCCCAGTTCATCAGCAACTTGCTTCTTGCTGTTATGACGTGAAAGCGCCTCGCGGATCATTTGCTTTTCCATCTCCTCCAGCGCCGTgcc                                     >  1:38822/1‑140 (MQ=255)                                                                                       tttAACAACTCATATTTCTTAATCTTGCGATAGAGCGTGGCAATGCCGATGCCCAGTTCATCAGCAACTTGCTTCTTGCTGTTATGACGTGAAAGCGCCTCGCGGATCATTTGCTTTTCCATCTCCTCCAGCGCCGTgcc                                     >  1:347295/1‑140 (MQ=255)                                                                                       tttAACAACTCATATTTCTTAATCTTGCGATAGAGCGTGGCAATGCCGATGCCCAGTTCATCAGCAACTTGCTTCTTGCTGTTATGACGTGAAAGCGCCTCGCGGATCATTTGCTTTTCCATCTCCTCCAGCGCCGTgcc                                     <  2:90138/140‑1 (MQ=255)                                                                                         tAACAACTCATATTTCTTAATCTTGCGATAGAGCGTGGCAATGCCGATGCCCAGTTCATCAGCAACTTGCTTCTTGCTGTTATGACGTGAAAGCGCCTCGTGGATCATTTGCTTTTCAATCTTCTCCAGCGCCGTgccgc                                   >  2:244375/1‑140 (MQ=255)                                                                                         tAACAACTCATATTTCTTAATCTTGCGATAGAGCGTGGCAATGCCGATGCCCAGTTCATCAGCAACTTGCTTCTTGCTGTTATGACGTGAAAGCGCCTCGCGGATCATTTGCTTTTCCATCTCCTCCAGCGCCGTgccgc                                   >  2:423829/1‑140 (MQ=255)                                                                                         tAACAACTCATATTTCTTAATCTTGCGATAGAGCGTGGCAATGCCGATGCCCAGTTCATCAGCAACTTGCTTCTTGCTGTTATGACGTGAAAGCGCCTCGCGGATCATTTGCTTTTCCATCTCCTCCAGCGCCGTgccgc                                   >  1:560523/1‑140 (MQ=255)                                                                                         tAACAACTCATATTTCTTAATCTTGCGATAGAGCGTGGCAATGCCGATGCCCAGTTCATCAGCAACTTGCTTCTTGCTGTTATGACGTGAAAGCGCCTCGCGGATCATTTGCTTTTCCATCTCCTCCAGCGCCGTgccgc                                   <  1:560070/140‑1 (MQ=255)                                                                                         tAACAACTCATATTTCTTAATCTTGCGATAGAGCGTGGCAATGCCGATGCCCAGTTCATCAGCAACTTGCTTCTTGCTGTTATGACGTGAAAGCGCCTCGCGGATCATTTGCTTTTCCATCTCCCCCAGCGCCGTgccgc                                   >  1:267274/1‑140 (MQ=255)                                                                                           acaacTCATATTTCTTAATCTTGCGATAGAGCGTGGCAATGCCGATGCCCAGTTCATCAGCAACTTGCTTCTTGCTGTTATGACGTGAAAGCGCCTCGCGGATCATTTGCTTTTCCATCTCCTCCAGCGCCGTGccgccc                                 <  1:116823/140‑1 (MQ=255)                                                                                            caacTCATATTTCTTAATCTTGCGATAGAGCGTGGCAATGCCGATGCCCAGTTCATCAGCAACTTGCTTCTTGCTGTTATGACGTGAAAGCGCCTCGCGGATCATTTGCTTTTCAATCTCCTCCAGCGCCGTGccgcccg                                >  1:210100/1‑140 (MQ=255)                                                                                               cTCATATTTCTTAATTTTGCGAGAGAGCGTGGCAATGTCGATGCCCAGTTCATCAGCAACTTGCTTCTTGCTGTTATGACGTGAAAGCGCCTCGCGGATCATTTGCTTTTCCATCTCCTCCAGCGCCGTGCCGCCCGcat                             <  1:244375/140‑1 (MQ=255)                                                                                               cTCATATTTCTTAATCTTGCGATAGAGCGTGGCAATGCCGATGCCCAGTTCATCAGCAACTTGCTTCTTGCTGTTATGACGTGAAAGCGCCTCGCGGATCATTTGCTTTTCCATCTCCTCCAGCGCCGTGCCGCCCGcat                             <  2:560523/140‑1 (MQ=255)                                                                                                  atatTTCTTAATCTCGCTAATGTTCGTTGCAAATCCGAAGCCCAATTCTTTAGTAACGTTGTTATTGGTGTTATGACGTGAAAGCGCCTCGCGGATCATTTGCTTGTCCATCACCTCCAGCGCCGTGCCGCCCGcatcat                          <  2:54889/140‑1 (MQ=255)                                                                                                          tAATCTTGCGATAGAGCGTGGCAATGCCGATGCCCAGTTCATCAGCAACTTGCTTCTTGCTGTTATGACGTGAAAGCGCCTCGCGGATCATTTGCTTTTCCATCTCCTCCAGCGCCGTGCCGCCCGCATCATCGAGTGAc                  >  2:502017/1‑140 (MQ=255)                                                                                                             tcgcaagATTGAGCGTGGCAATGCCGATGCCCAGTTCATCAGCAACTTGCTTCTTGCTGTTATGACGTGAAAGCGCCTCGCGGATCATTTGCTTTTCCATCTCCTCCAGCACCGAGCCGCCCGCGTCATCGAGTGACAgg               >  1:269563/7‑140 (MQ=255)                                                                                                                 gCGATAGAGCGTGGCAATGCCGATGCCCAGTTCATCAGCAACTTGCTTCTTGCTGTTATGACGTGAAAGCGCCTCGCGGATCATTTGCTTTTCCATCTCCTCCAGCGCCGTGCCGCCCGCATCATCGAGTGACAGGTgcg           >  2:441219/1‑140 (MQ=255)                                                                                                                 gCGATAGAGCGTGGCAATGCCGATGCCCAGTTCATCAGCAACTTGCTTCTTGCTGTTATGACGTGAAAGCGCCTCGCGGATCATTTGCTTTTCCATCTCCTCCAGCGCCGTGCCGCCCGCATCATCGAGTGACAGGTgcg           >  1:419586/1‑140 (MQ=255)                                                                                                                  cGATAGAGCGTGGCAATGCCGATGCCCAGTTCATCAGCAACTTGCTTCTTGCTGTTATGACGTGAAAGCGCCTCGCGGATCATTTGCTTTTCCATCTCCTCCGGCGCCGTCCCGGCCCCATCATCGAGTGACAGGTgcgc          >  1:251529/1‑140 (MQ=255)                                                                                                                  cGATAGAGCGTGGCAATGCCGATGCCCAGTTCATCAGCAACTTGCTTCTTGCTGTTATGACGTGAAAGCGCCTCGCGGATCATTTGCTTTTCCATCTCCTCCAGCGCCGTGCCGACCGCATCATGGAGTGACAGGTgcgc          >  1:297313/1‑140 (MQ=255)                                                                                                                  cGATAGAGCGTGGCAATGCCGATGCCCAGTTCATCAGCAACTTGCTTCTTGCTGTTATGACGTGAAAGCGCCTCGCGGATCATTTGCTTTTCCATCTCCTCCAGCGCCGTACCGCCCGCATCATCGAGTGACAGGTgcgc          >  1:256802/1‑140 (MQ=255)                                                                                                                   gATAGAGCGTGGCAATGCCGATGCCCAGTTCATCAGCAACTTGCTTCTTGCTGTTATGACGTGAAAGCGCCTCGCGGATCATTTGCTTTTCCATCTCCTCCAGCGCCGTGCCGCCCGCATCATCGAGTGACAGGTGCGcc         <  2:38822/140‑1 (MQ=255)                                                                                                                        agCGTGGCAATGCCGATGCCCAGTTCATCAGCAACTTGCTTCTTGCTGTTATGACGTGAAAGCGCCTCGCGGATCATTTGCTTTTCCATCTCCTCCAGCGCCGTGCCGCCCGCATCAGCGAGTGACAGGTGCGCCTCACt    <  2:502485/140‑1 (MQ=255)                                                                                                                          cGTGGCAATGCCGATGCCCAGTTCATCAGCAACTTGCTTCTTGCTGTTATGACGTGAAAGCGCCTCGCGGATCATTTGCTTTTCCATCTCCTCCAGCGCCGTGCCGCCCGCATCATCGAGTGACAGGTGCGCCTCACTgc  >  2:107056/1‑139 (MQ=255)                                                                                                                             |                                                                                                                                         GATTGTAAATACAGTTCTGGATTGCGAGAAAACGAAGTAAAAGGATATCCGGCCTGAATTCAGGCCGGATTCACTGAGGTTATGTGTTTAACAACTCATATTTCTTAATCTTGCGATAGAGCGTAGCAATGCCGATGCCCAGTTCATCAGCAACTTGCTTCTTGCTGTTATGACGTGAAAGCGCCTCGCGGATCATTTGCTTTTCCATCTCCTCCAGCGCCGTGCCGCCCGCATCATCGAGTGACAGGTGCGCCTCACTGA  >  NC_000913/3003929‑3004189

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑