Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,011,406	T→C	V460A (GTG→GCG)	hyuA →	phenylhydantoinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,011,406	0	T	C	100.0%	105.2 / NA	30	V460A (GTG→GCG)	hyuA	phenylhydantoinase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (8/22);  total (8/22)

GCCATGGGAGGGTTTTACCTGTCAGGGCGCGATTGTCAGAACCTTATCCCGTGGTGAAACGATTTTCTGTGACGGCACCTTTACAGGCAAAGCCGGGCGAGGTCGTTTCCTGCGACGCAAACCGTTTGTCCCTCCCGTGCTCTAACCACTGCCAGGGATCTATACACAATCAAGAATGATGAGTGAGGAAAAATGAGTAAGAAAATTGTTCTCGCCCTGGGCGGGAATGCGCTGGGCGATGACCTGGCCGGGCAAATGAAAGCGGTAAA  >  NC_000913/3011269‑3011537                                                                                                                                          |                                                                                                                                    gCCATGGGAGGGTTTTACCTGTCAGGGCGCGATTGTCAGAACCTTATCCCGTGGTGAAACGATTTTCTGTGACGGCACCTTTACAGGCAAAGCCGGGCGAGGTCGTTTCCTGCGACGCAAACCGTTTGTCCCTCCCgcgc                                                                                                                                   >  2:382068/1‑140 (MQ=255)       ggAGGGTTTTACCTGTCAGGGCGCGATTGTCAGAACCTTATCCCGTGGTGAAACGATTTTCTGTGACGGCACCTTTACAGGCAAAGCCGGGCGAGGTCGTTTCCTGCGACGCAAACCGTTTGTCCCTCCCGCGCTCTAAc                                                                                                                             <  1:416952/140‑1 (MQ=255)         aGGGTTTTACCTGTCGGGGCGCAATTGTCAGAACCTTTTCCCGTGGTGAAACGATTTTCTGTGACGGCACCTTTACAGGCAAAGCCGGGCGAGGTCGTTTCCTGCGACGCAAACCGTTTGTCCCTCCCGCGCTCTAACCa                                                                                                                           <  1:224182/140‑1 (MQ=255)          gggTTTTACCTGTCAGGGCGCGATTGTCAGAACCTTATCCCGTGGTGAAACGATTTTCTGTGACGGCACCTTTACAGGCAAAGCCGGGCGAGGTCGTTTCCTGCGACGCAAACCGTTTGTCCCTCCCGCGCTCTAACCAc                                                                                                                          <  1:150909/140‑1 (MQ=255)          gggTTTTACCTGTCAGGGCGCGATTGTCAGAACCTTATCCCGTGGTGAAACGATTTTCTGTGACGGCACCTTTACAGGCAAAGCCGGGCGAGGTCGTTTCCTGCGACGCAAACCGTTTGTCCCTCCCGCGCTCTAACCAc                                                                                                                          <  1:382068/140‑1 (MQ=255)               ttACCTGTCAGGGCGCGCTTGTGAGAACCTTCTCCGGTGGTGAAACGAGTTTCTGTGACGGCACCTTTACAGGCAAAGCCGGGCGAGGTCGTTTCCTGCGACGCAAACCGTTTGTCCCTCCCGCGCTCTAACCACTGCCa                                                                                                                     <  2:231973/140‑1 (MQ=255)               ttACCTGTCAGGGCGCGATTGTGAGAACCTTATCCCGTGGTGAAACGATTTTCTGTGACGGCACCTTTACAGGCAAAGCCGGGCGAGGTCGTTTCCTGCGACGCAAACCGTTTGTCCCTCCCGCGCTCTAACCACTGCCa                                                                                                                     <  1:298499/140‑1 (MQ=255)               ttACCTGTCAGGGCGCGATTGTCAGAACCTTATCCCGTGGTGAAACGATTTTCTGTGACGGCACCTTTACAGGCAAAGCCGGGCGAGGTCGTTTCCTGCGACGCAAACCGTTTGTCCCTCCCGCGCTCTAACCACTGCCa                                                                                                                     <  2:497800/140‑1 (MQ=255)                 gccTGCCAGGGCGCCATAGTCAAAACCTTATCCCGTGGTCAAGCGATTCTCTATGACGGCCCCTGTCCAGGCACGCCCGGGCGAGGTCGTTTCCTGAGCCGCAAACCGTTTGTCCCTCCCGCGCTCTAACCACTGCCAgg                                                                                                                   <  2:225402/139‑1 (MQ=255)                 aCCTGTCAGGGCGCGATTGTCAGAACCTTATACCGAGGTCAAACGATTTTCTGTGACGGCACCTTTACAGGCAAAGCCGGGCGAGGTCGTTTCCTGCGACGCAAACCGTTTGTCCCTCCCGCGCTCTAACCACTGCCAgg                                                                                                                   <  2:257955/140‑1 (MQ=255)                    tGTCAGGGCGCGATTGTCAGAACCTTATCCCGTGGTGAAACGATTTTCTGTGACGGCACCTTTACAGGCAAAGCCGGGCGAGGTCGTTTCCTGCGACGCAAACCGTTTGTCCCTCCCGCGCTCTAACCACTGCCAGGGAt                                                                                                                <  2:426363/140‑1 (MQ=255)                    tGTCAGGATGCGCTTGTCAGAACTTTATCCCGTGGTGAAACGATTTTCTGTGACGGCACCTTTACAGGCAAAGCCGGGCGAGGTCGTTTCCTGCGACGCAAACCGTTTGTCCCTCCCGCGCTCTAACCACTGCCAGGGAt                                                                                                                <  2:622008/140‑1 (MQ=255)                      tCAGGGCGCGATTGTCAGAACCTTATCCCGTGGTGAAACGATTTTCTGTGACGGCACCTTTACAGGCAAAGCCGGGCGAGGTCGTTTCCTGCGACGCAAACCGTTTGTCCCTCCTGCCCTCTAATCACTGCCAGGGATCt                                                                                                              >  1:245371/1‑140 (MQ=255)                          ggCGCGATTGTCAGAACCTTATCCCGTGGTGAAACGATTTTCTGTGACGGCACCTTTACAGGCAAAGCCGGGCGAGGTCGTTTCCTGCGACGCAAACCGTTTGTCCCTCCCGCGCTCTAACCACTGCCAGGGATCTATac                                                                                                          <  1:132407/140‑1 (MQ=255)                           gcgcGATTGTCAGGACCTTATCCCGTGGTGAAACGATTTTCTGTGACGGCACCTTTACAGGCAAAGCCGGGCGAGGTCGTTTCCTGCGACGCAAACCGTTTGTCCCTCCCGCGCTCTAACCACTGCCAGGGATCTATaca                                                                                                         <  1:498924/140‑1 (MQ=255)                            cgcgATTGTCAGAACCTTATCCCGTGGTGAAACGATTTTCTGTGACGGCACCTTTACAGGCAAAGCCGGGCGAGGTCGTTTCCTGCGACGCAAACCGTTTGTCCCTCCCGCGCTCTAACCACTGCCAGGGATCTATacac                                                                                                        >  1:408482/1‑140 (MQ=255)                                aTTGTCAGAACCTTATCCCGTGGTGAAACGATTTTCTGTGACGGCACCTTTACAGGCAAAGCCGGGCGAGGTCGTTTCCTGCGACGCAAACCGTTTGTCCCTCCCGCGCTCTAACCACTGCCAGGGATCTATACACAATc                                                                                                    >  2:79217/1‑140 (MQ=255)                                 ttGTCAGAACCTTATCCTGTGGGGAAACGATTTTCTGTGACGGCACCTTTACAGGCAAAGCAGGGCGAGGTCGTTTCCTGCGACGCAAACCGTTTGTCCCTCCCGCGCTCTAACCACTGCCAGGGATCTATACACAATCa                                                                                                   <  2:283918/140‑1 (MQ=255)                                 tcgTCAGAACCTTATCCCGTGGTGAAACGACTTTCTGTGACGGCCCCTTTACAGGCACAGCCGGGCGAGGTCGTTTCCTGCGACGCAAACCGTTTGTCCCTCCCGCGCTCTAACCACTGCCAGGGATCTATACACAATCa                                                                                                   <  1:416452/138‑1 (MQ=255)                                         aCCTTATCCCGTGGTGAAACGATTTTCTGTGACGGCACCTTTACAGGCAAAGCCGGGCGAGGTCGTTTCCTGCGACGCAAACCGTTTGTCCTTCCCGCGCCCTAGCCACTGCCAGGGGTCTATACACAATCAAGAatgat                                                                                           >  2:275686/1‑140 (MQ=255)                                             tATCCCGTGGTGAAACGATTTTCTGTGACGGCACCTTTACAGGCAAAGCCGGGCGAGGTCGTTTCCTGCGACGCAAACCGTTTGTCCCTCCGGCGCTCTAGCCAGTGCGAGGGATCTTTACAGAATCAAGAATGAtgagt                                                                                       >  2:170752/1‑140 (MQ=255)                                              aTCCCGTGGTGAAACGATTTTCTGTGACGGCACCTTTACAGGCAAAGCCGGGCGAGGTCGTTTCCTGCGACGCAAACCGTTTGTCCCTCCCGCGCTCTAACCACTGCCAGGGATCTATACACAATCAAGAATGAtgagtg                                                                                      <  1:444402/140‑1 (MQ=255)                                                     ggtgAAACGATTTTCTGTGACGGCACCTTTACAGGCAAAGCCGGGCGAGGTCGTTTCCTGCGACGCAAACCGTTTGTCCCTCCCGCGCTCTAACCACTGCCAGGGATCTATACACAATCAAGAATGATGAGTGAGGaaaa                                                                               <  2:137839/140‑1 (MQ=255)                                                      gtgAAACGATTTTCTGTGACGGCACCTTTACAGGCAAAGCCGGGCGAGGTCGTTTCCTGCGACGCAAACCGTTTGTCCCTCCCGCGCTCTAACCACTGCCAGGGATCTATACACAATCAAGAATGATGAGTGAGGaaaaa                                                                              <  1:133336/140‑1 (MQ=255)                                                             gATTTTCTGTGACGGCACCTTTACAGGCAAAGCCGGGCGAGGTCGTTTCCTGCGACGCAAACCGTTTGTCCCTCCCGCGCTCTAACCACTGCCAGGGATCTATACACAATCAAGAATGATGAGTGAGGAAAAATGAGTaa                                                                       <  1:502427/140‑1 (MQ=255)                                                                             aCCTTTACAGGCAAACCCGGGCTAGTTCGTTTCCTGCTACGCAAACCGTTTGTCCCTCCCGCGCTCTAACCACTGCCAGGGATCTATACACAATCAAGAATGATGAGTGAGGAAAAATGAGTAAGAAAATTGTTCTCGcc                                                       <  1:275686/140‑1 (MQ=255)                                                                                         aaaGCCGGGCGAGGTCGTTTCCTGCGACGCAAACCGTTTGTCCCTCCCGCGCTCTAACCACTGCCAGGGATCTATACACAATCAAGAATGACGAGTGAGGCAAATTTACTAAGAAAATTGTTCTCGCCCTGGGCGGGAAt                                           >  2:296323/1‑140 (MQ=255)                                                                                         aaaGCCGGGCGAGGT‑GTTTCCTGCGACGCAAACCGTTTGTCCCTCCCGCGCTCTAACCACTGCCAGGGATCTATACACAATCAAGAATGATGAGTGAGGAAAAATGAGTAAGAAAATTGTTCTCGCCCTGGGCGGGAATg                                          >  1:404032/1‑140 (MQ=255)                                                                                                 gCGAGGTCGTTTCCTGCGACGCAAACCGTTTGTCCCTCCCGCGCTCTAACCACTGCCAGGGATCTATACACAATCAAGAATGATGAGTGAGGAAAAATGAGTAAGAAAATTGTTCTCGCCCTGGGCGGGAATGCGCTggg                                   >  1:555070/1‑140 (MQ=255)                                                                                                   gAGGTCGTTTCCTGCGACGCAAACCGTTTGTCCCTCCCGCGCTCTAACCACTGCCAGGGATCTATACACAATCAAGAATGATGAGTGAGGAAAAATGAGTAAGAAAATTGTTCTCGCCCTGGGCGGGAATGCGCTGGGCg                                 <  2:119584/140‑1 (MQ=255)                                                                                                                                 tccctcccGCGCTCTAACCACTGCCAGGGATCTATACACAATCAAGAATGATGAGTGAGGAAAAATGAGTAAGAAAATTGTTCTCGCCCTGGGCGGGAATGCGCTGGGCGATGACCTGGCCGGGCAAATGAAAGCGGTaa   <  1:420411/140‑1 (MQ=255)                                                                                                                                  ccctcccGCGCTCTAACCACTGCCAAGGATCTATACACAATCAAGAATGATGAGTGAGGAAAAATGAGTAAGAAAATTGTTCTCGCCCTGGGCGGGAATGCGCTGGGCGATGACCTGGCCGGGCAAATGAAAGCGGTaaa  <  1:296323/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                    GCCATGGGAGGGTTTTACCTGTCAGGGCGCGATTGTCAGAACCTTATCCCGTGGTGAAACGATTTTCTGTGACGGCACCTTTACAGGCAAAGCCGGGCGAGGTCGTTTCCTGCGACGCAAACCGTTTGTCCCTCCCGTGCTCTAACCACTGCCAGGGATCTATACACAATCAAGAATGATGAGTGAGGAAAAATGAGTAAGAAAATTGTTCTCGCCCTGGGCGGGAATGCGCTGGGCGATGACCTGGCCGGGCAAATGAAAGCGGTAAA  >  NC_000913/3011269‑3011537

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑