Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,058,859	T→C	Y156C (TAC→TGC)	rpiA ←	ribose‑5‑phosphate isomerase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,058,859	0	T	C	100.0%	98.5 / NA	29	Y156C (TAC→TGC)	rpiA	ribose‑5‑phosphate isomerase A
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (11/18);  total (11/18)

ACAAGCCAACAGTCACCACGCCAGGAATCGCATTTATGGCGTTTTCCATCGCTATCGGGTCAAGGATTTCCATGCCGTGGACGTCGAGGATCACGTTGCCATTATCGGTCACCACGCCCTGACGGTATTCCGGACGACCGCCCAGTTTCACCAGCTGACGCGCCACTGCACTACGTGCCATCGGGATAACTTCTACTGGCAGCGGGAATTTACCCAGAATATCAACCTGCTTGGAAGCGTCTGCAATACAG  >  NC_000913/3058734‑3058984                                                                                                                              |                                                                                                                              aCAAGCCAACAGTCACCACGCCAGGAATCGCATTTATGGCGTTTTCCATCGCTATCGGGTCAAGGATTTCCATGCCGTGGACGTCGAGGATCACGTTGCCATTATCGGTCACCACGCCCTGACGGCATTCCGGACGACCg                                                                                                                 <  1:83919/140‑1 (MQ=255)      ccAACAGTCACCACGCCAGGAATCGCATTTATGGCGTTTTCCATCGCTATCGGGTCAAGGATTTCCATGCCGTGGACGTCGAGGATCACGTTGCCATTATCGGTCACCACGCCCTGACGGCATTCCGGACGACCGCCCAg                                                                                                            >  2:534686/1‑140 (MQ=255)             tCACCACGCCAGGAATCGCATTTATGGCGTTTTCCATCGCTATCGGGTCAAGGATTTCCATGCCGTGGACGTCGAGGATCACGTTGCCGTTATCGGTCACCACGCCCTGACGGCATTCCGGACGACCGCCCAGTTTCAcc                                                                                                     >  1:111117/1‑140 (MQ=255)               accacGCCAGGAATCGCATTTATGGCGTTTTCCATCGCTATCGGGTCAAGGATTTCCATGCCGTGGACGTCGAGGATCACGTTGCCATTATCGGTCACCACGCCCTGACGGCATTCCGGACGACCGCCCAGTTTCACCAg                                                                                                   >  2:633509/1‑140 (MQ=255)                       aGGAATCGCATTTATGGCGTTTTCCATCGCTATCGGGTCAAGGATTTCCATGCCGTGGACGTCGAGGATCACGTTGCCATTATCGGTCACCACGCCCTGACGGCATTCCGGACGACCGCCCAGTTTCACCAGCTGAcgcg                                                                                           <  2:419165/140‑1 (MQ=255)                       aGGAATCGCATTTATGGCGTTTTCCATCGCTATCGGGTCAAGGATTTCCATGCCGTGCACGTCGAGGATCACGTTGCCATTATCGGTCACCACGCCCTGACGGCATTCCGGACGACCGCCCAGTTTCACCAGCTGAcgcg                                                                                           <  2:408969/140‑1 (MQ=255)                        ggAATCGCATTTATGGCGTTTTCCATCGCTATCGGGTCAAGGATTTCCATGCCGTGGACGTCGAGGATCACGTTGCCATTATCGGTCACCACGCCCTGACGGCATTCCGGACGACCGCCCAGTTTCACCAGCTGACgcgc                                                                                          <  1:534686/140‑1 (MQ=255)                        ggAATCGCATTTATGACGTTTTCCATCGCTATCGGGTTAAGGATTTCCATGCCGTGGACGTCGAGGATCACGTTGCCATTATCGGTCACCACGCCCTGACGGCATTCCGGACGACCGCCCAGTTTCACCAGCTGACgcgc                                                                                          <  1:281331/140‑1 (MQ=255)                        ggAATCGCATTTATGACGTTTTACAGCGCCATCGGGTCAAGGATTTCCATGCCGTGGACGTCGAGGATCACGTTGCCATTATCGGTCACCACGCCCTGACGGCATTCCGGACGACCGCCCAGTTTCACCAGCTGACgcgc                                                                                          <  1:314923/140‑1 (MQ=255)                          aaccGCATTTATGGCGTTTTCCATCGCTATCGGGTCAAGGATTTCCATGCCGTGGACGTCGAGGATCACGTTGCCATTATCGGTCACCACGCCCTGACGGCATTCCGGACGACCGCCCAGTTTCACCAGCTGACGCGCCa                                                                                        <  2:400885/137‑1 (MQ=255)                            tCGCATTTATGGCGTTTTCCATCGCTATCGGGTCAAGGATTTCCATGCCGTGGACGTCGAGGATCACGTTGCCATTATCGGTCACCACGCCCTGACGGCATTCCGGACGACCGCCCAGTTTCACCAGCTGACGCGCCACt                                                                                      <  2:460127/140‑1 (MQ=255)                                        cGTTTTCCATCGCTATCGGGTCAAGGATTTCCATGCCGTGGACGTCGAGGATCACGTTGCCATTATCGGTCACCACGCCCTGACGGCATTCCGGACGACCGCCCAGTTTCACCAGCTGACGCGCCACTGCACTACGTGcc                                                                          <  2:411682/140‑1 (MQ=255)                                        cGTTTTCCATCGCTATCGGGTCAAGGATTTCCATGCCGTGGACGTCGAGGATCACGTTGCCATTATCGGTCACCACGCCCTGACGGCATTCCGGACGACCGCCCAGTTTCACCAGCTGACGCGCCACTGCACTACGTGcc                                                                          <  2:380776/140‑1 (MQ=255)                                              ccATCGCTATCGGGTCAAGGATTTCCATGCCGTGGACGTCGAGGATCACGTTGCCATTATCGGTCACCACGCCCTGACGGCATTCCGGACGACCGCCCAGTTTCACCAGCTGACGCGCCACTGCACTACGTGCCATCggg                                                                    <  1:543246/140‑1 (MQ=255)                                                     tATCGGGTCAAGGATTTCCATGCCGTGGACGTCGAGGATCACGTTGCCATTATCGGTCACCACGCCCTGACGGCATTCCGGACGACCGCCCAGTTTCACCAGCTGACGCGCCACTGCACTACg                                                                              >  2:58801/1‑123 (MQ=255)                                                     tATCGGGTCAAGGATTTCCATGCCGTGGACGTCGAGGATCACGTTGCCATTATCGGTCACCACGCCCTGACGGCATTCCGGACGACCGCCCAGTTTCACCAGCTGACGCGCCACTGCACTACg                                                                              <  1:58801/123‑1 (MQ=255)                                                       tCGGGTCAAGGATTTCCATGCCGTGGACGTCGAGGATCACGTTGCCATTATCGGTCACCACGCCCTGACGGCATTCCGGACGACCGCCCAGTTTCACCAGCTGACGCGCCACTGCACTACGTGCCATCGGGATAACTTCt                                                           >  1:547934/1‑140 (MQ=255)                                                                     tCCATGCCGTGGACGTCGAGGATCACGTTGCCATTATCGGTCACCACGCCCTGACGGCATTCCGGACGACCGCCCAGTTTCACCAGCTGACGCGCCACTGCACTACGTGCCATCGGGATAACTTCTACTGGCAGCGGGaa                                             >  2:490729/1‑140 (MQ=255)                                                                           ccGTGGACGTCGAGGATCACGTTGCCATTATCGGTCACCACGCCCTGACGGCATTCCGGACGACCGCCCAGTTTCACCAGCTGACGCGCCACTGCACTACGTGCCATCGGGATAACTTCTACTGGCAGCGGGAATTTAcc                                       >  1:51505/1‑140 (MQ=255)                                                                             gTGGACGTCGAGGATCACGTTGCCATTATCGGTCACCACGCCCTGACGGCATTCCGGACGACCGCCCAGTTTCACCAGCTGACGCGCCACTGCACTACGTGCCATCGGGATAACTTCTACTGGCAGCGGGAATTTACCCa                                     <  1:633509/140‑1 (MQ=255)                                                                             gTGGACGTCGAGGATCACGTTGCCATTATCGGTCACCACGCCCTGACGGCATTCCGGACGACCGCCCAGTTTCACCAGCTGACGCGCCACTGCACTACGTGCCATCGGGATAACTTCTACTGGCAGCGGGAATTTACCCa                                     <  2:133772/140‑1 (MQ=255)                                                                                gACGTCGAGGATCACGTTGCCATTATCGGTCACCACGCCCTGACGGCATTCCGGACGACCGCCCAGTTTCACCAGCTGACGCGCCACTGCACTACGTGCCATCGGGATAACTTCTACTGGCAGCGGGAATTTACCCAGaa                                  >  2:30049/1‑140 (MQ=255)                                                                                       aGGATCACGTTGCCATTATCGGTCACCACGCCCTGACGGCATTCCGGACGACCGCCCAGTTTCACCAGCTGACGCGCCACTGCACTACGTGCCATCGGGATAACTTCTACTGGCAGCGGGAATTTACCCAGAATATCAAc                           <  2:68007/140‑1 (MQ=255)                                                                                       aGGATCACGTTGCCATTATCGGTCACCACGCCCTGACGGCATTCCGGACGACCGCCCAGTTTCACCAGCTGACGCGCCACTGCACTACGTGCCATCGGGATAACTTCTACTGGCAGCGGGAATTTACCCAGAATATCAAc                           <  1:490729/140‑1 (MQ=255)                                                                                       aGGATCACGTTGCCACTATCGGACAGCACGCCCTGACGGCATTCCGGACGACCGCCCAGTTTCACCAGCTGACGCGCCACTGCACTACGTGCCATCGGGATAACTTCTACTGGCAGCGGGAATTTACCCAGAATATCAAc                           <  1:284114/140‑1 (MQ=255)                                                                                             aCGTTGCCATTATCGGTCACCACGCCCTGACGGCATTCCGGACGACCGCCCAGTTTCACCAGCTGACGCGCCACTGCACTACGTGCCATCGGGATAACTTCTACTGGCAGCGGGAATTTACCCAGAATATCAACCTGCtt                     >  1:123999/1‑140 (MQ=255)                                                                                                           ggTCACCACGCCCTGACGGCATTCCGGACGACCGCCCAGTTTCACCAGCTGACGCGCCACTGCACTACGTGCCATCGGGATAACTTCTACTGGCAGCGGGAAGTTACCCATAATATCGACCTGCCTGGAAGCGTCTGCaa       >  1:200952/1‑140 (MQ=255)                                                                                                             tCACCACGCCCTGACGGCATTCCGGACGACCGCCCAGTTTCACCAGCTGACGCGCCACTGCACTACGTGCCATCGGGATAACTTCTACTGGCAGCGGGAATTTACCCAGAATATCAACCTGCTTGGAAGCGTCTGCAATa     >  1:405716/1‑140 (MQ=255)                                                                                                                ccacGCCCTGACGGCATTCCGGACGACCGCCCAGTTTCACCAGCTGACGCGCCACTGCACTACGTGCCATCGGGATAACTTCTACTGGCAGCGGGAATTTACCCAGAATATCAACCTGCTTGGAAGCGTCTGCAATACAg  <  1:477738/140‑1 (MQ=255)                                                                                                                              |                                                                                                                              ACAAGCCAACAGTCACCACGCCAGGAATCGCATTTATGGCGTTTTCCATCGCTATCGGGTCAAGGATTTCCATGCCGTGGACGTCGAGGATCACGTTGCCATTATCGGTCACCACGCCCTGACGGTATTCCGGACGACCGCCCAGTTTCACCAGCTGACGCGCCACTGCACTACGTGCCATCGGGATAACTTCTACTGGCAGCGGGAATTTACCCAGAATATCAACCTGCTTGGAAGCGTCTGCAATACAG  >  NC_000913/3058734‑3058984

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑