Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,060,042	T→C	V97A (GTC→GCC)	argP →	DNA‑binding transcriptional dual regulator ArgP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,060,042	0	T	C	100.0%	72.9 / NA	23	V97A (GTC→GCC)	argP	DNA‑binding transcriptional dual regulator ArgP
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (8/15);  total (8/15)

TGCGTACCGTACCGCCGCGCCCGACGGAACAAGGGCAAAAACTGCTGGCACTGCTGCGCCAGGTGGAGTTGCTGGAAGAAGAGTGGCTGGGCGATGAACAAACCGGTTCGACTCCGCTGCTGCTTTCACTGGCGGTCAACGCCGACAGTCTGGCGACGTGGTTGCTTCCTGCACTGGCTCCTGTGTTGGCTGATTCGCCTATCCGCCTCAACTTGCAGGTAGAAGATGAAACCCGCACTCAGGAACGTCTGCGCCGCGGCGAAGTGGT  >  NC_000913/3059907‑3060174                                                                                                                                        |                                                                                                                                     tGCGTACCGTACCGCCGCGCCCGACGGAACAAGGGCAAAAACTGCTGGCACTGCTGCGCCAGGTGGAGTTGCTGGAAGAAGAGTGGCTGGGCGATGAACAAACCGGTTCGACTCCGCTGCTGCTTTCACTGGCGGCCAAc                                                                                                                                  >  2:373817/1‑140 (MQ=255)      accgtaccgCCGCGCCCGACGGAACAAGGGCAAAAACTGCTGGCACTGCTGCGCCAGGTGGAGTTGCTGGAAGAAGAGTGGCTGGGCGATGAACAAACCGGTTCGACTCCGCTGCTGCTTTCACTGGCGGCCAACGCCGa                                                                                                                             <  2:503698/140‑1 (MQ=255)               ccgcGCCCGACGGAACAAGGGCAAAAACTGCTGGCACTGCTGCGCCAGGTGGAGTTGCTGGAAGAAGAGTGGCTGGGCGATGAACAAACCGGTTCGACTCCGCTACCGCCTTCACTGGCGGCGCACGCCGAGAGTCTGGc                                                                                                                    >  2:312617/1‑140 (MQ=255)                cgcgCCCGACGGAACAAGGGCAAAAACTGCTGGCACTGCTGCGCCAGGTGGAGTTGCTGGAAGAAGAGTGGCTGGGCGATGAACAAACCGGCTCGACTCCGCTGCTGCTTTCACTGGTGGCCGACGCCGACAGTCTGGcc                                                                                                                   >  2:246090/1‑139 (MQ=255)                        aCGGAACAAGGGCAAAAACTGCTGGCACTGCTGCGCCAGGTGGAGTTGCTGGAAGAAGAGTGGCTGGGCGATGAACAAACCGGTTCGACTCCGCTGCTGCTTTCACTGGCGGCCAACGCCGACAGTCTGGCGACGTGGtt                                                                                                           <  2:150137/140‑1 (MQ=255)                                    cAAAAACTGCTGGCACTGCTGCGCCAGGTGGAGTTGCTGGAAGAAGAGTGGCTGGGCGATGAACAAACCGGTTCGACTCCGCTGCTGCTTTCACTGGCGGCCAACGCCGACAGTCTGGCGACGTGGTTGCTTCCTGCACt                                                                                               <  1:79470/140‑1 (MQ=255)                                                           ccAGGTGGAGTTGCTGGAAGAAGAGTGGCTGGGCGATGAACAAACCGGTTCGACTCCGCTGCTGCTTTCACTGGCGGCCAACGCCGACAGTCTGGCGACGTGGTTGCTTCCTGCACTGGCTCCTGTGTTGGCTGATTCGc                                                                        >  2:74323/1‑140 (MQ=255)                                                           ccAGGTGGAGTTGCTGGAAGAAGAGTGGCTGGGCGATGAACAAACCGGTTCGACTCCGCTGCTGCTTTCACTGGCGGCCAACGCCGACAGTCTGGCGACGTGGTTGCTTCCTGCACTGGCTCCTGTGTTGGCTGATTCGc                                                                        >  2:480096/1‑140 (MQ=255)                                                                      tGCTGGAAGAAGAGTGGCTGGGCGATGAACAAACCGGTTCGACTCCGCTGCTGCTTTCACTGGCGGCCAACGCCGACAGTCTGGCGACGTGGTTGCTTCCTGCACTGGCTCCTGTGTTGGCTGATTCGCCTATCCGCCTc                                                             <  2:23740/140‑1 (MQ=255)                                                                      tGCTGGAAGAAGAGTAGCTGGGCGATGAACAAACCGGCTCGACTCCGCTGCTGCTTTCACTGGCGGCCAACGCCGACAGTCTGGCGACGTGGTTGCTTCCTGCACTGGCTCCTGTGTTGGCTGATTCGCCTATCCGCCTc                                                             <  1:237329/140‑1 (MQ=255)                                                                        cTGGAAGAAGAGTGGCTGGGCGATGAACAAACCGGTTCGACTCCGCTGCTGCTTTCACTGGCGGCCAACGCCGACAGTCTGGCGACGTGGTTGCTTCCTGCACTGGCTCCTGTGTTGGCTGATTCGCCTATCCGCCTCaa                                                           >  1:137956/1‑140 (MQ=255)                                                                            aagaagAGTGGCTGGGCGATGAACAAACCGGTTCGACTCCGCTGCTGCTTTCACTGGCGGCCAACGCCGACAGTCTGGCGACGTGGTTGCTTCCTGCACTGGCTCCTGTGTTGGCTGATTCGCCTATCCGCCTCAACTTg                                                       <  2:132589/140‑1 (MQ=255)                                                                            aagaagAGTGGCTGGGCGATGAACAAACCGGTTCGACTCCGCTGCTGCTTTCACTGGCGGCCAACGCCGACAGTCTGGCGACGTGGTTGCTTCCTGCACTGGCTCCTGTGTTGGCTGATTCGCCTATCCGCCTCAACTTg                                                       <  1:398634/140‑1 (MQ=255)                                                                             agaagaGTGGCTGGGCGATGAACAAACCGGTTCGACTCCGCTGCTGCTTTCACTGGCGGCCAACGCCGACAGTCTGGCGACGTGGTTGCTTCCTGCACTGGCTCCTGTGTTGGCTGATTCGCCTATCCGCCTCAACTTGc                                                      <  2:623024/140‑1 (MQ=255)                                                                              gaagaGTGGCTGGGCGATGAACAAACCGGTTCGACTCCGCTGCTGCTTTCACTGGCGGCCAACGCCGACAGTCTGGCGACGTGGTTGCTTCCTGCACTGGCTTCTGTGTTAGCTAATGCGCCTAGCCGCCTCAACTTGCa                                                     >  1:203083/1‑140 (MQ=255)                                                                                  agTGGCTGGGCGATGAACAAACCGGTTCGACTCCGCTGCTGCTTTCACTGGCGGCCAACGCCGACAGTCTGGCGACGTGGTTGCTTCCTGCACTGGCTCCTGTGTTGGCTGATTCGCCTATCCGCCTCAACTTGCAGGTa                                                 >  2:52701/1‑140 (MQ=255)                                                                                   gTGGCTGGGCGATGAACAAACCAGTTCGACTCCGCAGCTTCTTTCACTAGCGGCCAACGCCGACAGTCTGGCGACGTGGTTGCTTCCTGCACTGGCTCCTGTGTTGGCTGATTCGCCTATCCGCCTCAACTTGCAGGTag                                                <  2:291395/140‑1 (MQ=255)                                                                                        tGGGCGATGAACAAACCGGTTCGACTCCGCTGCTGCTTTCACTGGCGGCCAACGCCGACAGTCTGGCGACGTGGTTGCTTCCTGCACTGGCTCCTGTGTTGGCTGATTCGCCTATCCGCCTCAACTTGCAGGTAGAAGAt                                           <  2:574828/140‑1 (MQ=255)                                                                                         gggCGATGAACAAACCGGTTCGACTCCGCTGCTGCTTTCACTGGCGGCCAACGCCGACAGTCTGGCGACGTGGTTGCTTCCTGCACTGGCTCCTGTGTTGGCTGATTCGCCTATCCGCCTCAACTTGCAGGTAGAAGATg                                          >  2:363731/1‑140 (MQ=255)                                                                                                 aaCAAACCGGTTCGATTCCGCTCCTGCTTTCACTGGCAGCCAACGCCGAAAGTCTGGCGACGTGGTTGCTTCCTGCACTGGCTCCTGTGTTGGCTGATTCGCCTATCCGCCTCAACTTGCAGGTAGAAGATGAAACCCGc                                  <  1:261500/140‑1 (MQ=255)                                                                                                 aaCAAACCGGTTCGACTCCGCTGCTGCTTTCACTGGCGGCCAACGCCGACAGTCTGGCGACGTGGTTGCTTCCTGCACTGGCTCCTGTGTTGGCTGATTCGCCTATCCGCCTCAACTTGCAGGTAGAAGATGAAACCCGc                                  <  1:97577/140‑1 (MQ=255)                                                                                                    aaaCCGGTTCGACTCCGCTGCTGCTTTCACTGGCGGCCAACGCCGACAGTCTGGCGACGTGGTTGCTTCCTGCACTGGCTCCTGTGTTGGCTGATTCGCCTATCCGCCTCAACTTGCAGGTAGAAGATGAAACCCGCACt                               <  1:480096/140‑1 (MQ=255)                                                                                                     aaCCGGTTCGACTCCTCTGCTGGTTCCACGGGCGGCCCACGCCGACAGTCTGGCGACGTGGTTGCTTCCTGCACTGGCTCCTGTGTTGGCTGATTCGCCTATCCGCCTCAACTTGCAGGTAGAAGATGAAACCCGCACTc                              <  1:246090/140‑1 (MQ=255)                                                                                                           ttCGACTCCGCTGCTGCTTTCACTGGCGGCCAACGCCGACAGTCTGGCGACGTGGTTGCTTCCTGCACTGGCTCCTGTGTTGGCTGATTCGCCTATCCGCCTCAACTTGCAGGTAGAAGATGAAACCCGCACTCAGGAAc                        >  1:406767/1‑140 (MQ=255)                                                                                                                          gctGTCACTGGCGGCAAGCGCAGACTGTCTGGCGACGTGGTTGCTTCTTGCCCTTGCTCCTGTGTTGGCTGATTCGCCTATCCGCCTCAACTTGCAGGTAGAAGATGAAACCCGCACTCAGGAACGTCTGCGCCgcggcg         <  2:549720/140‑1 (MQ=255)                                                                                                                                 cTGGCGGCCAACGCCGACAGTCTGGCGACGTGGTTGCTTCCTGCACTGGCTCCTGTGTTGGCTGATTCGCCTATCCGCCTCAACTTGCAGGTAGAAGATGAAACCCGCACTCAGGAACGTCTGCGCCGCGGCGAAGTGGt  <  1:44734/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                     TGCGTACCGTACCGCCGCGCCCGACGGAACAAGGGCAAAAACTGCTGGCACTGCTGCGCCAGGTGGAGTTGCTGGAAGAAGAGTGGCTGGGCGATGAACAAACCGGTTCGACTCCGCTGCTGCTTTCACTGGCGGTCAACGCCGACAGTCTGGCGACGTGGTTGCTTCCTGCACTGGCTCCTGTGTTGGCTGATTCGCCTATCCGCCTCAACTTGCAGGTAGAAGATGAAACCCGCACTCAGGAACGTCTGCGCCGCGGCGAAGTGGT  >  NC_000913/3059907‑3060174

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑