Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,063,040	G→T	E18D (GAG→GAT)	argK →	methylmalonyl‑CoA mutase‑interacting GTPase YgfD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,063,040	0	G	T	100.0%	132.0 / NA	41	E18D (GAG→GAT)	argK	methylmalonyl‑CoA mutase‑interacting GTPase YgfD
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (25/16);  total (25/16)

GCGGCGTGGCGGCGATTTATGGTCCAGGTACACCTATGCTCGACAGTGTGCGCGACGTACTGAATCTGATAAGCCAGCATCATGATTAATGAAGCCACGCTGGCAGAAAGTATTCGCCGCTTACGTCAGGGTGAGCGTGCCACACTCGCCCAGGCCATGACGCTGGTGGAAAGCCGTCACCCGCGTCATCAGGCACTAAGTACGCAGCTGCTTGATGCCATTATGCCGTACTGCGGTAACACCCTGCGACTGGGCGTTACCGGCACCCC  >  NC_000913/3062906‑3063174                                                                                                                                       |                                                                                                                                       gcggcgTGGCGGCGATTTATGGTCCAGGTACACCTATGCTCGACAGTGTGCGCGACGTACTGAATCTGATAAGCCAGCATCATGATTAATGAAGCCACGCTGGCAGAAAGTATTCGCCGCTTACGTCAGGGTGATCGTGc                                                                                                                                   >  2:104146/1‑140 (MQ=255)                           ggTACACCTATGCTCGACAGTGTGCGCGACGTACTGAATCTGATAAGCCAGCATCATGATTAATGAAGCCACGCTGGCAGAAAGTATTCGCCGCTTACGTCAGGGTGATCGTGCCACACTCGCCCAGGCCATGACGCtgg                                                                                                         >  1:367356/1‑140 (MQ=255)                               cacCTATGTTCGACAGTGTGCGCGACGTACTGAATCTGATAAGCCAGCATCATGATTAATGAAGCCACGCTGGCAGAAAGTATTCGCCGCTTACGTCAGGGTGATCGTGCCACACTCGCCCAGGCCATGACGCTGGTGGa                                                                                                     <  1:498776/140‑1 (MQ=255)                               cacCTATGCTCGACAGTGTGCGCGACGTACTGAATCTGATAAGCCAGCATCATGATTAATGAAGCCACGCTGGCAGAAAGTATTCGCCGCTTACGTCAGGGTGATCGTGCCACACTCGCCCAGGCCATGACGCTGGTGGa                                                                                                     <  2:450972/140‑1 (MQ=255)                               cacCTATGCTCGACAGTCTGCGCGACGTACTCAATCTGATAAGCCAGCCTCATGATTAATGAAGCCACGCTGGCAGAAAGTATTCGCCGCTTACGTCAGGGTGATCGTGCCACACTCGCCCAGGCCATGACGCTGGTGGa                                                                                                     <  2:186638/140‑1 (MQ=255)                                     tGCTCGACAGTGTGCGCGACGTACTGAATCTGATAAGCCAGCATCATGATTAATGAAGCCACGCTGGCAGAAAGTATTCGCCGCTTACGTCAGGGTGATCGTGCCACACTCGCCCAGGCCATGACGCTGGTGGAAAGCCg                                                                                               >  2:407608/1‑140 (MQ=255)                                      gCTCGACAGTGTGCGCGACGTACTGAATCTGATAAGCCAGCATCATGATTAATGAAGCCACGCTGGCAGAAAGTATTCGCCGCTTACGTCAGGGTGATCGTGCCACACTCGCCCAGGCCATGACGCTGGTGGAAAGCCGt                                                                                              >  1:336142/1‑140 (MQ=255)                                       cTCGACAGTGTGCGCGACGTACTGAATCTGATAAGCCAGCATCATGATTAATGAAGCCACGCTGGCAGAAAGTATTCGCCGCTTACGTCAGGGTGATCGTGCCACACTCGCCCAGGCCATGACGCTGGTGGAAAGCCGTc                                                                                             >  1:131808/1‑140 (MQ=255)                                           aCAGTGTGCGCGACGTACTGAATCTGATAAGCCAGCATCATGATTAATGAAGCCACGCTGGCAGAAAGTATTCGCCGCTTACGTCAGGGTGATCGTGCCACACTCGCCCAGGCCATGACGCTGGTGGAAAGCCGTCAccc                                                                                         <  2:142024/140‑1 (MQ=255)                                            cAGTGTGCGCGACGTACTGAATCTGATAAGCCAGCATCATGATTAATGAAGCCACGCTGGCAGAAAGTATTCGCCGCTTACGTCAGGGTGATCGTGCCACACTCGCCCAGGCCATGACGCTGGTGGAAAGCCGTCACCcg                                                                                        >  2:632238/1‑140 (MQ=255)                                            cAGTGTGCGCGACGTACTGAATCTGATAAGCCAGCATCATGATTAATGAAGCCACGCTGGCAGAAAGTATTCGCCGCTTACGTCAGGGTGATCGTGCCACACTCGCCCAGGCCATGACGCTGGTGGAAAGCCGTCACCcg                                                                                        >  1:586977/1‑140 (MQ=255)                                            cAGTGTGCGCGACGTACTGAATCTGATAAGCCAGCATCATGATTAATGAAGCCACGCTGGCAGAAAGTATTCGCCGCTTACGTCAGGGTGATCGTGCCACACTCACCCGGGCCATGAAGCTGGTGGAAAGCCGTCACCcg                                                                                        >  2:257182/1‑140 (MQ=255)                                            cAGTGTGCGCGACGTACTGAATCTGATAAGCCAGCATCATGATTAATGAAGCCACGCTGGCAGAAAGTATTCGCCGCTTACGTCAGGGTGATCGTCCCACACTCGCCCAGGCCATGACGCTGGTGGAAAGCCGTCACCcg                                                                                        >  1:516971/1‑140 (MQ=255)                                               tgtgCGCGACGTACTGAATCTGATAAGCCAGCATCATGATTAATGAAGCCACGCTGGCAGAAAGTATTCGCCGCTTACGTCAGGGTGATCGTGCCACACTCGCCCAGGCCATGACGCTGGTGGAAAGCCGTCACCCGCGt                                                                                     >  2:23733/1‑140 (MQ=255)                                                  gcgcGACGTACTGAATCTGATAAGCCAGCATCATGATTAATGAAGCCACGCTGGCAGAAAGTATTCGCCGCTTACGTCAGGGTGATCGTGCCACACTCGCCCAGGCCATGACGCTGGTGGAAAGCCGTCACCCGCGtcat                                                                                  <  1:609631/140‑1 (MQ=255)                                                          agcTGAATGTGATAAGCAAGCAACATGATTTTTAAAGCCCCGCTGGCAAAAAGTATTCGCCGCTTACGTCAGGGTGATCGTGCCACACTCGCCCAGGCCATGACGCTGGTGGAAAGCCGTCACCCGCGTCATCAGGCACt                                                                          <  2:328018/138‑1 (MQ=255)                                                           aCTGAATCTGATAAGCCAGCATCATGATTAATGAAGCCACGCTGGCAGAAAGTATTCGCCGCTTACGTCAGGGTGATCGTGCCACACTCGCCCAGGCCATGACGCTGGTGGAAAGCCGTCACCCGCGTCATCAGGCACTa                                                                         >  1:456628/1‑140 (MQ=255)                                                           aCTGAATCTGATAAGCCAGCATCATGATTAATGAAGCCACGCTGGCAGAAAGTATTCGCCGCTTACGTCAGGGTGATCGTGCCACACTCGCCCAGGCCATGAAGCTGGTGGAAAGCCATCACCCACGTCATCAGGCACTa                                                                         >  1:204381/1‑140 (MQ=255)                                                                 tCTGATAAGCCAGCATCATGATTAATGAAGCCACGCTGGCAGAAAGTATTCGCCGCTTACGTCAGGGTGATCGTGCCACACTCGCCCAGGCCATGACGCTGGTGGAAAGCCGTCACCCGCGTCATCAGGCACTAAGTACg                                                                   <  1:598605/140‑1 (MQ=255)                                                                    gATAAGCCAGCATCATGATTAATGAAGCCACGCTGGCAGAAAGTATTCGCCGCTTACGTCAGGGTGATCGTGCCACACTCGCCCAGGCCATGACGCTGGTGGAAAGCCGTCACCCGCGtcat                                                                                  <  2:84766/122‑1 (MQ=255)                                                                    gATAAGCCAGCATCATGATTAATGAAGCCACGCTGGCAGAAAGTATTCGCCGCTTACGTCAGGGTGATCGTGCCACACTCGCCCAGGCCATGACGCTGGTGGAAAGCCGTCACCCGCGtcat                                                                                  >  1:84766/1‑122 (MQ=255)                                                                     aTAAGCCAGCATCATGATTAATGAAGCCACGCTGGCAGAAAGTATTCGCCGCTTACGTCAGGGTGATCGTGCCACACTCGCCCAGGCCATGACGCTGGTGGAAAGCCGTCACCCGCGTCATCAGGCACTAAGTACGCAgc                                                               >  2:338050/1‑140 (MQ=255)                                                                       aaGCCAGCATCATGATTAATGAAGCCACGCTGGCAGAAAGTATTCGCCGCTTACGTCAGGGTGATCGTGCCACACTCGCCCAGGCCATGACGCTGGTGGAAAGCCGTCACTCGCATCCTCAGGCACTAAGTACGCAgcct                                                             >  1:328018/1‑138 (MQ=255)                                                                         gCCAGCATCATGATTAATGAAGCCACGCTGGCAGAAAGTATTCGCCGCTTACGTCAGGGTGATCGTGCCACACTCGCCCAGGCCATGACGCTGGTGGAAAGCCGTCACCCGCGTCATCAGGCACTAAGTACGCAgctgct                                                           >  2:147727/1‑140 (MQ=255)                                                                         gCCAGCATCATGATTAATGAAGCCACGCTGGCAGAAAGTATTCGCCGCTTACGTCAGGGTGATCGTGCCACACTCGCCCAGGCCATGACGCTGGTGGAAAGCCGTCACCCGCGTCATCAGGCACTAAGTACGCAgctgct                                                           >  2:458797/1‑140 (MQ=255)                                                                             gCATCATGATTAATGAAGCCACGCTGGCAGAAAGTATTCGCCGCTTACGTCAGGGTGATCGTGCCACACTCGCCCAGGCCATGACGCTGGTGGAAAGCCGTCACCCGCGTCATCAGGCACTAAGTACGCAGCTGCTTGAt                                                       <  2:456628/140‑1 (MQ=255)                                                                              catcatGATTAATGAAGACACGATGGCAGAACGTATTCGTCGCTTACGTCAGGGTGATCGTGCCACACTCGCCCAGGCCATGACGCTGGTGGAAAGCCGTCACCCGCGTCATCAGGCACTAAGTACGCAGCTGCTTGATg                                                      <  2:249219/140‑1 (MQ=255)                                                                                 catGATTAATGAAGCCACGCTGGCAGAAAGTATTCGCCGCTTACGTCAGGGTGATCGTGCCACACTCGCCCAGGCCATGACGCTGGTGGAAAGCCGTCTCCCGCGTCATCAGGCACTAAGTACGCAGCTGCTTGATGCCa                                                   <  1:406227/140‑1 (MQ=255)                                                                                 catGATTAATGAAGCCACGCTGGCAGAAAGTATTCGCCGCTTACGTCAGGGTGATCGTGCCACACTCGCCCAGGCCATGACGCTGGTGGAAAGCCGTCACCCGCGTCATCAGGCACTAAGTACGCAGCTGCTTGATGCCa                                                   <  1:469489/140‑1 (MQ=255)                                                                                   tGATTAATGAAGCCACGCTGGCAGAAAGTATTCGCCGCTTACGTCAGGGTGATCGTGCCACACTCGCCCAGGCCATGACGCTGGTGGAAAGCCGTCACCCGCGTCATCAGGCACTAAGTACGCAGCTGCTTGATGCCAtt                                                 >  1:96066/1‑140 (MQ=255)                                                                                    gATTAATGAAGCCACGCTGGCAGAAAGTATTCGCCGCTTACGTCAGGGTGATCGTGCCACACTCGCCCAGGCCATGACGCTGGTGGAAAGCTGTCACCCGCGTCGTCATGCACTAAGGACGCAGCTGCTTGATGCCATTa                                                >  2:279107/1‑140 (MQ=255)                                                                                      ttAATGAAGCCACGCTGGCAGAAAGTATTCGCCGCTTACGTCAGGGTGATCGTGCCACACTCGCCCAGGCCATGACGCTGGTGGAAAGCCGTCACCCGCGTCATCAGGCACTAAGTACGCAGCTGCTTGATGCCATTATg                                              <  1:372311/140‑1 (MQ=255)                                                                                            aaGCCACGCTGGCAGAAAGTATTCGCCGCTTACGTCAGGGTGATCGTGCCACACTCGCCCAGGCCATGACGCTGGTGGAAAGCCGTCACCCGCGTCATCAGGCACTAAGTACGCAGCTGCTTGATGCCATTATGCCGTAc                                        <  2:336142/140‑1 (MQ=255)                                                                                            aaGCCACGCTGGCAGAAAGTATTCGCCGCTTACGTCAGGGTGATCGTGCCACACTCGCCCAGGCCATGACGCTGGTGGAAAGCCGTCACCCGCGTCATCAGGCACTAAGTACGCAGCTGCTTGATGCCATTATGCCGTAc                                        <  1:407608/140‑1 (MQ=255)                                                                                            aaGCCACGCTGGCAGAAAGTATTCGCCGCTTACGTCAGGGTGATCGTGCCACACTCGCCCAGGCCATGACGCTGGTGGAAAGCCGTCACCCGCGTCATCAGGCACTAAGTACGCAGCTGCTTGATGCCATTATGCCGTAc                                        <  1:104146/140‑1 (MQ=255)                                                                                              gCCACGCTGGCAGAAAGTATTCGCCGCTTACGTCAGGGTGATCGTGCCACACTCGCCCAGGCCATGACGCTGGTGGAAAGCCGTCACCCGCGTCATCAGGCACTAAGTACGCAGCTGCTTGATGCCATTATGCCGTACTg                                      >  2:610507/1‑140 (MQ=255)                                                                                              gCCACGCTGGCAGAAAGTATTCGCCGCTTACGTCAGGGTGATCGTGCCACACTCGCCCAGGCCATGACGCTGGTGGAAAGCCGTCACCCGCGTCATCAGGAACTCAGTCCGCAGCTGGTTGACGCCATTATGCCGTACTg                                      >  2:263200/1‑140 (MQ=255)                                                                                              gCCACGCTGGCAGAAAGTATTCGCCGCTTACGTCAGGGTGATCGTGCCACACTCGCCCAGGCCATGACGCTGGTGGAAAGCCGTCACCCGCCTCATCAGGAACTAAGTCCGCAGCTGTTTGAGGCCATTATGCCGTACTg                                      >  2:202082/1‑140 (MQ=255)                                                                                               ccACGCTGGCAGAAAGTATTCGCCGCTTACGTCAGGGTGATCGTGCCACACTCGCCCAGGCCATGACGCTGGTGGAAAGCCGTCACCCGCGTCATCAGGCACTAAGTACGCAGCAGCGTGATGCCATTATGCCGTACTGc                                     >  1:295379/1‑140 (MQ=255)                                                                                                             aGTATTCGCCGCTTACGTCAGGGTGATCGTGCCACACTCGCCCAGGCCATGACGCTGGTGGAAAGCCGTCACCCGCGTCATCAGGCACTAAGTACGCAGCTGCTCGATGCCATTATGCCGTACTGCGGTAACACCCTGCg                       >  2:266687/1‑140 (MQ=255)                                                                                                                                  ggTGATCGTGCCACACTCGCCCAGGCCATGACGCTGGTGGAAAGCCGTCACCCGCGTCATCAGGCACTAAGTACGCAGCTGCTTGATGCCATTATGCCGTACTGCTGTAACACCCTGCGACTGGGCGTTACCGGCAcccc  >  1:111883/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                       GCGGCGTGGCGGCGATTTATGGTCCAGGTACACCTATGCTCGACAGTGTGCGCGACGTACTGAATCTGATAAGCCAGCATCATGATTAATGAAGCCACGCTGGCAGAAAGTATTCGCCGCTTACGTCAGGGTGAGCGTGCCACACTCGCCCAGGCCATGACGCTGGTGGAAAGCCGTCACCCGCGTCATCAGGCACTAAGTACGCAGCTGCTTGATGCCATTATGCCGTACTGCGGTAACACCCTGCGACTGGGCGTTACCGGCACCCC  >  NC_000913/3062906‑3063174

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑