Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,085,503	A→G	S137P (TCC→CCC)	speA ←	arginine decarboxylase, biosynthetic

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,085,503	0	A	G	100.0%	138.3 / NA	40	S137P (TCC→CCC)	speA	arginine decarboxylase, biosynthetic
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (20/20);  total (20/20)

GGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGAACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACGCGGCGGTGCTGGTTAACTTTGATCGGATAAACAAGGAAGTAATCGCCGTTATAGCCGTAGGATTCCCTCGCACGTTTGAACGCGGCGTTAATGGAACGCAAACGGTGCTGCAGGATCTGTG  >  NC_000913/3085368‑3085634                                                                                                                                        |                                                                                                                                    ggCGGATATATTCGCGGTCTTTATAACCGTTACAGACGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCCTTGGAACAGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAGGAGCTCa                                                                                                                                 >  2:11950/1‑140 (MQ=255)     gATATATTCGCGGTCTTTATAACCGTTGCAGACGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGAACCGGCTTCCAGACCCAGCGGTTCGCCC‑‑‑‑‑AATCAGGGGCTCAATCACgcgg                                                                                                                        <  2:634943/140‑1 (MQ=255)       tatatTCGCGGTCTTTATAACCGTTGCAGACGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGAACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAGGGGCTCAATCAcg                                                                                                                           >  2:92415/1‑140 (MQ=255)          atTCGCGGTCTTTATAACCGTTGCAGACGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGAACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAGGGGCTCAATCACgcgg                                                                                                                        <  2:465394/140‑1 (MQ=255)               cgGTCTTTATAACCGTTTCAGAAGATGACGCCACGTGTCTTGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGAACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAGGGGCTCAATCACGCGGCGGTg                                                                                                                   <  2:175674/140‑1 (MQ=255)               cgGTCTTTATAACCGTTGCAGACGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGAACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAGGGGCTCAATCACGCGGCGGTg                                                                                                                   <  2:33191/140‑1 (MQ=255)                ggTCTTTATAACCGTTGCAGACGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGAACCGGCTTCCAGACCCAGCGGTTAGCCCGAACGAAACATGGGCTCGATCACGCGGCGGTGc                                                                                                                  >  1:190976/1‑140 (MQ=255)                    tttATAACCGTTGCAGACGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGAACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAGGGGCTCAATCACGCGGCGGTGCTGGt                                                                                                              <  2:64246/140‑1 (MQ=255)                            cGTTGCAGACGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGAACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAGGGGCTCAATCACGCGGCGGTGCTGGTTAACTTTg                                                                                                      >  2:523815/1‑140 (MQ=255)                            cGTTGCAGACGATGACGCTACGGGCCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGAACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAGGGGCTCAATCACGCGGCGGTGCTGGTTAACTTTg                                                                                                      >  2:659994/1‑140 (MQ=255)                              ttGCAGACGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGAACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAGGGGCTCAATCACGCGGCGGTGCTGGTTAACTTTGAt                                                                                                    >  1:445258/1‑140 (MQ=255)                               tGCAGACGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGAACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAGGGGCTCAATCACGCGGCGGTGCTGGTTAACTTTGATc                                                                                                   <  1:523815/140‑1 (MQ=255)                                gCAGACGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGAACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAGGGGCTCAATCACGCGGTGGTGCTGGTTAACTTTGATCg                                                                                                  >  1:409996/1‑140 (MQ=255)                                gCAGACGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGAACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAGGGGCTCAATCACGCGGCGGTGCTGGTTAACTTTGATCg                                                                                                  >  2:150116/1‑140 (MQ=255)                                 cAGACGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGAACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAGGGGCTCAATCACGCGGCGGTGCTGGTTAACTTTGATCgg                                                                                                 <  1:531316/140‑1 (MQ=255)                                         gACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGAACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAGGGGCTCAATCACGCGGCGGTGCTGGTTAACTTTGATCGGATAAACaa                                                                                         >  1:368433/1‑140 (MQ=255)                                           cGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGAACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAGGGGCTCAATCACGCGGCGGTGCTGGTTAACTTTGATCGGATAAACAAgg                                                                                       >  2:518092/1‑140 (MQ=255)                                           cGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGAACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAGGGGCTCAATCACGCGGCGGTGCTGGTTAACTTTGATCGGATAAACAAgg                                                                                       >  2:543301/1‑140 (MQ=255)                                                   gTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGAACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAGGGGCTCAATCACGCGGCGGTGCTGGTTAACTTTGATCGGATAAACAAGGAAGTAATc                                                                               <  1:10837/140‑1 (MQ=255)                                                   gTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGAACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAGGGGCTCAATCACGCGGCGGTGCTGGTTAACTTTGATCGGATAAACAAGGAAGTAATc                                                                               <  2:445258/140‑1 (MQ=255)                                                    tCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGAACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAGGGGCTCAATCACGCGGCGGTGCTGGTTAACTTTGATCGGATAAACAAGGAAGTAATCg                                                                              <  1:409987/140‑1 (MQ=255)                                                           aGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGAACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAGGGGCTCAATCACGCGGCGGTGCTGGTTAACTTTGATCGGATAAACAAGGAAGTAATCGCCGTtat                                                                       <  2:466975/140‑1 (MQ=255)                                                             cATGTGCCAGTACTGCCATCAACTCGGCTTTGGAACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAGGGGCTCAATCACGCGGCGGTGCTGGTTAATTTTGATCGGATGAATAAGGAAATAATCGCCGTTATAg                                                                     >  1:194263/1‑140 (MQ=255)                                                             cATGTGCCAGTACTGCCATCAACTCGGCTTTGGAACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAGGGGCTCAATCACGCGGCGGTGCTGGTTAACTTTGATCGGATAAACAAGGAAGTAATCGCCGTTATAg                                                                     >  2:523572/1‑140 (MQ=255)                                                                           gCCATCAACTCGGCTTTGGAACCGGCTTCCAGACACAGCGGTTCGCCCGAATGAATCAGGGGCTCAATCACGCGGCGGTGCTGGTTAACTTTGATCGGATAAACAAGGAAGTAATCGCCGTTATAGCCGTAGGATTCCCt                                                       >  2:40113/1‑140 (MQ=255)                                                                                 aaCTCGGCTTTGGAACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAGGGGCTCAATCACGCGGCGGTGCTGGTTAACTTTGATCGGATAAACAAGGAAGTAATCGCCGTTATAGcc                                                                   >  1:429785/1‑122 (MQ=255)                                                                                 aaCTCGGCTTTGGAACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAGGGGCTCAATCACGCGGCGGTGCTGGTTAACTTTGATCGGATAAACAAGGAAGTAATCGCCGTTATAGcc                                                                   <  2:429785/122‑1 (MQ=255)                                                                                   cTCGGCTTTGGAACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAGGGGCTCAATCACGCGGCGGTGCTGGTTAACTTTGATCGGATAAACAAGGGAGTTATCACCGTTATAACCGTTGGATTCCCTCGCACGtt                                               >  2:182048/1‑140 (MQ=255)                                                                                      ggCTTTGGAACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAGGGGCTCAATCACGCGGCGGTGCTGGGTAACTTTGATCGGATAAACAAGGAAGTAATCGCCGTTATAGCCGTAGGATTCCCTCGCACGTTTGa                                            >  1:667637/1‑140 (MQ=255)                                                                                       gCTTTGGAACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAGGGGCTCAATCACGCGGCGGTGCTGGTTAACTTTGATCGGATAAACAAGGAAGTAATCGCCGTTATAGCCGTAGGATTCCCTCGCACGTTTGaa                                           >  1:566978/1‑140 (MQ=255)                                                                                         tttGGAACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAGGGGCTCAATCACGCGGCGGTGCTGGTTAACTTTGATCGGATAAACAAGGAAGTAATCGCCGTTATAGCCGTAGGATTCCCTCGCACGTTTGAAcg                                         <  2:392745/140‑1 (MQ=255)                                                                                          ttGGAACCGGCTTCCAGACACAGCGGTTCGCCCGAATGAATCAGGGGCTCAATCACGCGGCGGTGCTGGTTAACTTTGATCGGATAAACAAGGAAGTAATCGCCGTTATAGCCGTAGGATTCCCTCGCACGTTTGAAcgc                                        <  1:40113/140‑1 (MQ=255)                                                                                          ttGGAACCCGCTTCCAGGCCCACCGGTTCGCACGAGTGACTCAGGGGCGCAATCACGCGGCGGTGCTGGTTAACTTTGATCGGATAAACAAGGAAGTAATCGCCGTTATAGCCGTAGGATTCCCTCGCACGTTTGAAcgc                                        <  2:313370/140‑1 (MQ=255)                                                                                           tGGAACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAGGGGCTCAATCACGCGGCGGTGCTGGTTAACTTTGATCGGATAAACAAGGAAGTAATCGCCGTTATAGCCGTAGGATTCCCTCGCACGTTTGAAcgcg                                       >  1:5718/1‑140 (MQ=255)                                                                                           tGGAACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAGGGGCTCAATCACGCGGCGGTGCTGGTTAACTTTGATCGGATAAACAAGGAAGTAATCGCCGTTATAGCCGTAGGATTCCCTCGCACGTTTGAAcgcg                                       >  1:511767/1‑140 (MQ=255)                                                                                                             ccAGCGGTTCGCCCGAATGAATCAGGGGCTCAATCACGCGGTGGTGCTGGTTAACTTTGATCGGATAAACAAGGAAGTAATCGCCGTTATAGCCGTAGGATTCCCTCGCACGTTTGAACGCGGCGTTAATGGAACGCaaa                     <  2:409996/140‑1 (MQ=255)                                                                                                                 cGGTTCGCCCGAATGAATCAGGGGCTCAATCACGCGGCGGTGCTGGTTAACTTTGATCGGATAAACAAGGAAGTAATCGCCGTTATAGCCGTAGGATTCCCTCGCACGTTTGAACGCGGCGTTAATGGAACGCAAACGGt                 <  2:368433/140‑1 (MQ=255)                                                                                                                     tCGCCCGAATGAATCAGGGGCTCAATCACGCGGCGGTGCTGGTTAACTTTGATCGGATAAACAAGGAAGTAATCGCCGTTATAGCCGTAGGATTCCCTCGCACGTTTGAACGCGGCGTTAATGGAACGCAAACGGtgctg             <  2:566978/140‑1 (MQ=255)                                                                                                                           gaatgaatCAGGGGCTCAATCACGCGGCGGTGCTGGTTAACTTTGATCGGATAAACAAGGAAGTAATCGCCGTTATAGCCGTAGGATTCCCTCGCACGTTTGAACGCGGCGTTAATGGAACGCAAACGGTGCTGCAGGAt       <  1:152738/140‑1 (MQ=255)                                                                                                                            aatgaatCAGGGGCTCAATCACGCGGCGGTGCTGGTTAACTTTGATCGGATAAACAAGGAAGTAATCGCCGTTATAGCCGTAGGATTCCCTCGCACGTTTGAACGCGGCGTTAATGGAACGCAAACGGTGCTGCAGGATc      >  1:420479/1‑140 (MQ=255)                                                                                                                                aatCAGGGGCTCAATCACGCGGCGGTGCTGGTTAACTTTGATCGGATAAACAAGGAAGTAATCGCCGTTATAGCCGTAGGATTCCCTCGCACGTTTGAACGCGGCGTTAATGGAACGCAAACGGTGCTGCAGGATCtgtg  <  2:30324/140‑1 (MQ=255)                                                                                                                                aatCAGGGGCTCAATCACGCGGCGGTGCTGGTTAACTTTGATCGGATAAACAAGGAAGTAATCGCCGTTATAGCCGTAGGATTCCCTCGCACGTTTGAACGCGGCGTTAATGGAACGCAAACGGTGCTGCAGGATCtgtg  <  1:150116/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                    GGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATGACGCTACGGGTCATGCCAGCATGTGCCAGTACTGCCATCAACTCGGCTTTGGAACCGGCTTCCAGACCCAGCGGTTCGCCCGAATGAATCAGGGACTCAATCACGCGGCGGTGCTGGTTAACTTTGATCGGATAAACAAGGAAGTAATCGCCGTTATAGCCGTAGGATTCCCTCGCACGTTTGAACGCGGCGTTAATGGAACGCAAACGGTGCTGCAGGATCTGTG  >  NC_000913/3085368‑3085634

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑