Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,089,017	A→G	N245S (AAC→AGC)	galP →	galactose:H(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,089,017	0	A	G	100.0%	142.7 / NA	40	N245S (AAC→AGC)	galP	galactose:H(+) symporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (13/27);  total (13/27)

TTTGTTGATGCCGAACGCGTGCTGCTACGCCTGCGTGACACCAGCGCGGAAGCGAAACGCGAACTGGATGAAATCCGTGAAAGTTTGCAGGTTAAACAGAGTGGCTGGGCGCTGTTTAAAGAGAACAGCAACTTCCGCCGCGCGGTGTTCCTTGGCGTACTGTTGCAGGTAATGCAGCAATTCACCGGGATGAACGTCATCATGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGC  >  NC_000913/3088893‑3089148                                                                                                                             |                                                                                                                                    tttGTTGATGCCGAACGCGTGCTGCTACGCCTGCGTGACACCAGCGCGGAAGCGAAACGCGAACTGGATGAAATCCGTGAAAGTTTGCAGGTTAAACAGAGTGGCTGGGCGCTGTTTAAAGAGAGCAGCAACTTccgccg                                                                                                                      <  1:41779/140‑1 (MQ=255)     ttgATGCCGAACGCGTGCTGCTACGCCTGCGTGACACCAGCGCGGAAGCGAAACGCGAACTGGATGAAATCCGTGAAAGTTTGCAGGTTAAACAGAGTGGCTGGGCGCTGTTTAAAGAGAGCAGCAACTTCCGCcgcgcg                                                                                                                  <  2:477678/140‑1 (MQ=255)            cGAACGCGTGCTGCTACGCCTGCGTGACACCAGCGCGGAAGCGAAACGCGAACTGGATGAAATCCGTGAAAGTTTGCAGGTTAAACAGAGTGGCTGGGCGCTGTTTAAAGAGAGCAGCAACTTCCGCCGCGCGGTGTTcc                                                                                                           <  1:535394/140‑1 (MQ=255)                    tgctgcTACGCCTGCGTGACACCAGCGCGGAAGCGAAACGCGAACTGGATGAAATCCGTGAAAGTTTGCAGGTTAAACAGAGTGGCTGGGCGCTGTTTAAAGAGAGCAGCAACTTCCGCCGCGCGGTGTTCCTTGGCGTa                                                                                                   <  2:626598/140‑1 (MQ=255)                       tgctACGCCTGCGTGACACCAGCGCGGAAGCGAAACGCGAACTGGATGAAATCCGTGAAAGTTTGCAGGTTAAACAGAGTGGCTGGGCGCTGTTTAAAGAGAGCAGCAACTTCCGCCGCGCGGTGTTCCTTGGCGTACTg                                                                                                <  2:547484/140‑1 (MQ=255)                              ccTGCGTGACACCAGCGCGGAAGCGAAACGCGAACTGGATGAAATCCGTGAAAGTTTGCAGGTTAAACAGAGTGGCTGGGCGCTGTTTAAAGAGAGCAGCAACTTCCGCCGCGCGGTGTTCCTTGGCGTACTGTTGCAgg                                                                                         <  1:74498/140‑1 (MQ=255)                                 gCGTGACACCAGCGCCGAAGCGTAACGCGCACTGGATCAAATCCGTGAAAGTTTGCAGGTTAAACAGAGTGGCTGGGCGCTGTTTAAAGAGAGCAGCAACTTCCGCCGCGCGGTGTTCCTTGGCGTACTGTTGCAGGTaa                                                                                      <  1:175860/140‑1 (MQ=255)                                   gTGACACCAGCGCGGAAGCGAAACGCGAACTGGATGAAATCCGTGAAAGTTTGCAGGTTAAACAGAGTGGCTGGGCGCTGTTTAAAGAGAGCAGCAACTTCCACCGCGCGGTGTTCCTTGGCGTACTGTTGCAGGTAATg                                                                                    >  1:290143/1‑140 (MQ=255)                                   gTGACACCAGCGCGGAAGCGAAACGCGAACTGGATGAAATCCGTGAAAGTTTGCAGGTTAAACAGAGTGGCTGGGCGCTGTTTAAAGAAAGCAGAAACTTCCGCCGCGCGGTGTTACTTGGCGTACTGTTCAAGGTAATg                                                                                    >  2:412753/1‑140 (MQ=255)                                            gcgcGGAAGCGAAACGCGAACTGGATGAAATCCGTGAAAGTTTGCAGGTTAAACAGAGTGGCTGGGCGCTGTTTAAAGAGAGCAGCAACTTCCGCCGCGCGGTGTTCCTTGGCGTACTGTTGCAGGTAATGCAGCAATTc                                                                           >  2:512298/1‑140 (MQ=255)                                             cgcgGAAGCGAAACGCGAACTGGATGAAATCCGTGAAAGTTTGCAGGTTAAACAGAGTGGCTGGGCGCTGTTTAAAGAGAGCAGCAACTTCCGCCGCGCGGTGTTCCTTGGCGTACTGTTGCAGGTAATGCAGCAATTCa                                                                          >  1:563744/1‑140 (MQ=255)                                                  aaGCGAAACGCGAACTGGATGAAATCCGTGAAAGTTTGCAGGTTAAACAGAGTGGCTGGGCGCTGTTTAAAGAGAGCAGCAACTTCCGCCGCGCGGTGTTCCTTGGCGTACTGTTGCAGGTAATGCAGCAATTCACCggg                                                                     <  1:52813/140‑1 (MQ=255)                                                      gAAACGCGAACTGGATGAAATCCGTGAAAGTTTGCAGGTTAAACAGAGTGGCTGGGCGCTGTTTAAAGAGAGTAGCAACTTCCGCCGCGCGGTGTTCCTTGGCGTACTGTTGCAGGTAATGCAGCAATTCACCGGGATGa                                                                 <  1:28500/140‑1 (MQ=255)                                                         aCGCGAACTGGATGAAATCCGTGAAAGTTTGCAGGTTAAACAGAGTGGCTGGGCGCTGTTTAAAGAGAGCAGCAACTTCCGCCGCGCGGTGTTCCTTGGCGTACTGTTGCAGGTAATGCAGCAATTCACCGGGATGAACg                                                              <  1:400476/140‑1 (MQ=255)                                                         aCGCGAACTGGATGAAATCCGTGAAAGTTTGCAGGTTAAACAGAGTGGCTGGGCGCTGTTTAAAGAGAGCAGCAACTTCCGCCGCGCGGTGTTCCTTGGCGTACTGTTGCAGGTAATGCAGCAATTCACCGGGATGAACg                                                              <  1:17891/140‑1 (MQ=255)                                                         aCGCGAACTGGATGAAATCCGTGAAAGTTTGCAGGTTAAACAGAGTGGCTGGGCGCTGTTTAAAGAGAGCAGCAACTTCCGCCGCGCGGTGTTCCTTGGCGTACTGTTGCAGGTAATGCAGCAATTCACCGGGATGAACg                                                              <  1:67624/140‑1 (MQ=255)                                                           gcgAACTGGTTGAAATCCGTGAAAGTTTGCAGGTTAAACAGATTGGCTGGGCGCTGTTTAAAGAGAGCAGCAACTTCCGCCGCGCGGTGTTCCTTGGCGTACTGTTGCAGGTAATGCAGCAATTCACCGGGATGAACGtc                                                            <  1:412753/140‑1 (MQ=255)                                                           gcgAACTGGATGAAATCCGTGAAAGTTTGCAGGTTAAACAGAGTGGCTGGGCGCTGTTTAAAGAGAGCAGCAACTTCCGCCGCGCGGTGTTCCTTGGCGTACTGTTGCAGGTAATGCAGCAATTCACCGGGATGAACGtc                                                            <  2:119844/140‑1 (MQ=255)                                                           gcgAACTGGATGAAATCCGTGAAAGTTTGCAGGTTAAACAGAGTGGCTGGGCGCTGTTTAAAGAGAGCAGCAACTTCCGCCGCGCGGTGTTCCTTGGCGTACTGTTGCAGGTAATGCAGCAATTCACCGGGATGAACGtc                                                            <  1:121630/140‑1 (MQ=255)                                                           gcgAACTGGATGAAATCCGTGAAAGTTTGCAGGTTAAACAGAGTGGCTGGGCGCTGTTTAAAGAGAGCAGCAACTTCCGCCGCGCGGTGTTCCTTGGCGTACTGTTGCAGGTAATGCAGCAATTCACCGGGATGAACGtc                                                            <  2:56220/140‑1 (MQ=255)                                                           gcgAACTGGATGAAATCAGTGAAAGTTTGCACGTTAAACTGAGTGGCTTGGCGCTGTTTAAAGAGAGCAGCAACTTCCGCCGCGCGGTGTTCCTTGGCGTACTGTTGCAGGTAATGCAGCAATTCACCGGGATGAACGtc                                                            <  2:248257/140‑1 (MQ=255)                                                            cgAACTGGATGAAATCCGTGAAAGTTTGCAGGTTAAACAGAGTGGCTGGGCGCTGTTTAAAGAGAGCAGCAACTTCCGCCGCGCGGTGTTCCTTGGCGTACTGTTGCAGGTAATGCAGCAATTCACCGGGATGAACGtca                                                           >  2:544831/1‑140 (MQ=255)                                                            cgAACTGGATGAAATCCGTGAAAGTTTGCAGGTTAAACAGAGTGGCTGGGCGCTGTTTAAAGAGAGCAGCAACTTCCGCCGCGCGGTGTTCCTTGGCGTACTGTTGCAGGTAATGCAGCAATTCACCGGGATGAACGtca                                                           <  1:27739/140‑1 (MQ=255)                                                             gAACTGGATGAAATCCGTGAAAGTTTGCAGGTTAAACAGAGTGGCTGGGCGCTGTTTAAAGAGAGCAGCAACTTCCGCCGCGCGGTGTTCCTTGGCGTACTGTTGCAGGTAATGCAGCAATTCACCGGGATGAACGtcat                                                          <  1:153566/140‑1 (MQ=255)                                                                          tCCGTGAAAGTTTGCAGGTTAAACAGAGTGGCTGGGCGCTGTTTAAAGAGAGCAGCAACTTCCGCCGCGCGGTGTTCCTTGGCGTACTGTTGCAGGTAATGCAGCAATTCACCGGGATGAACGTCATCATGTATTAcgcg                                             <  1:601924/140‑1 (MQ=255)                                                                                             tAAACAGAGTGGCTGGGCGCTGTTTAAAGAGAGCAGCAACTTCCGCCGCGCGGTGTTCCTTGGCGTACTGTTGCAGGTAATGCAGCAATTCACCGGGATGAACGTCATCATGTATTACGCGCCGAAAATCTTCGAACTgg                          <  2:19361/140‑1 (MQ=255)                                                                                             tAAACAGAGTGGCTGGGCGCTGTTTAAAGAGAGCAGCAACTTCCGCCGCGCGGTGTTCCTTGGCGTACTGTTGCAGGTAATGCAGCAATTCACCGGGATGAACGTCATCATGTATTACGCGCCGAAAATCTTCGAACTgg                          <  1:407533/140‑1 (MQ=255)                                                                                             tAAACAGAGTGGCTGGGCGCTGTTTAAAGAGAGCAGCAACTTCCGCCGCGCGGTGTTCCTTGGCGTACTGTTGCAGGTAATGCAGCAATTCACCGGGATGAACGTCATCATGTATTACGCGCCGAAAATCTTCGAACTgg                          <  2:563744/140‑1 (MQ=255)                                                                                                     gTGGCTGGGCGCTGTTTAAAGAGAGCAGCAACTTCCGCCGCGCGGTGTTCCTTGGCGTACTGTTGCAGGTAATGCAGCAATTCACCGGGATGAACGTCATCATGTATTACGCGCCGAAAATCTTCGAACTgg                          <  2:441773/132‑1 (MQ=255)                                                                                                     gTGGCTGGGCGCTGTTTAAAGAGAGCAGCAACTTCCGCCGCGCGGTGTTCCTTGGCGTACTGTTGCAGGTAATGCAGCAATTCACCGGGATGAACGTCATCATGTATTACGCGCCGAAAATCTTCGAACTgg                          >  1:441773/1‑132 (MQ=255)                                                                                                     gTGGCTGGGCGCTGTTTAAAGAGAGCAGCAACTTCCGCCGCGCGGTGTTCCTTGGCGTACTGTTGCAGGTAATGCAGCAATTCACCGGGATGAACGTCATCATGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTtat                  <  2:611560/140‑1 (MQ=255)                                                                                                      tGGCTGGGCGCTGTTTAAAGAGAGCAGCAACTTCCGCCGCGCGGTGTTCCTTGGCGTACTGTTGCAGGTAATGCAGCAATTCACCGGGATGAACGTCATCATGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTtata                 >  2:71568/1‑140 (MQ=255)                                                                                                      tGGCTGGGCGCCGTTTAAAGAGAGCAGCAACTTCCGCCGCGCGGTGTTCCTTGGCGTACTGTTGCAGGTAATGCAGCAATTCACCGGGATGAACGTCATCTTGTATTATGCGCCGAATATCTTCGAACTGGCGGGTtata                 >  2:258503/1‑140 (MQ=255)                                                                                                         cTGGGCACTGTTTAAAGAGAGCAGCAACTTCCGCCGCGCGGTGTTCCTTGGCGTACTGTTGCAGGTAATGCAGCAATTCACCGGGATGAACGTCATCATGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCa              >  2:479699/1‑140 (MQ=255)                                                                                                          tGGGCGCTGTTTAAAGAGAGCAGCAACTTCCGCCGCGCGGTGTTCCTTGGCGTACTGTTGCAGGTAATGCAGCAATTCACCGGGATGAACGTCATCATGTATTACGCGCCGAAAATCTTCGAACTGGCGGGGTATACCaa             >  2:366688/1‑140 (MQ=255)                                                                                                            ggCGCTGTTTAAAGAGAGCAGCAACTTCCGCCGCGCGGTGTTCCTTGGCGTACTGTTGCAGGTAATGCAGCAATTCACCGGGATGAACGTCATCATGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAaca           >  1:349251/1‑140 (MQ=255)                                                                                                              cgcTGTTTAAAGAGAGCAGCAACTTCCGCCGCGCGGTGTTCCTTGGCGTACTGTTGCAGGTAATGCAGCAATTCACCGGGATGAACGTCATCATGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACt         >  1:497437/1‑140 (MQ=255)                                                                                                              cgcTGTTTAAAGAGAGCAGCAACTTCCGCCGCGCGGTGTTCCTTGGCGTACTGTTGCAGGTAATGCAGCAATTCACCGGGATGAACGTCATCATGTATTACGCGCCGAAAATCTTCGAACTGGCGGGGTTTATCAACACt         >  2:581609/1‑140 (MQ=255)                                                                                                                     tAAAGAGAGCAGCAACTTCCGCCGCGCGGTGTTCCTTGGCGTACTGTTGCAGGTAATGCAGCAATTCACCGGGATGAACGTCATCATGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGc  <  2:349251/140‑1 (MQ=255)                                                                                                                     tAAAGAGAGCAGCAACTTCCGCCGCGCGGTGTTCCTTGGCGTACTGTTGCAGGTAATGCAGCAATTCACCGGGATGAACGTCATCATGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGc  <  1:71568/140‑1 (MQ=255)                                                                                                                             |                                                                                                                                    TTTGTTGATGCCGAACGCGTGCTGCTACGCCTGCGTGACACCAGCGCGGAAGCGAAACGCGAACTGGATGAAATCCGTGAAAGTTTGCAGGTTAAACAGAGTGGCTGGGCGCTGTTTAAAGAGAACAGCAACTTCCGCCGCGCGGTGTTCCTTGGCGTACTGTTGCAGGTAATGCAGCAATTCACCGGGATGAACGTCATCATGTATTACGCGCCGAAAATCTTCGAACTGGCGGGTTATACCAACACTACCGAGC  >  NC_000913/3088893‑3089148

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑