Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,141,483	T→C	T503A (ACC→GCC)	hybC ←	hydrogenase 2 large subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,141,483	0	T	C	100.0%	242.6 / NA	67	T503A (ACC→GCC)	hybC	hydrogenase 2 large subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (35/32);  total (35/32)

GCCATGCACGGGTCAAAGGAGTGAATGGTACGCACCACTTCCAGCGGTTTATTCGGATCGGCAACCGGTGTACCCACCAGCGACTGCTCGTAAGGACCGACGTCATCATTGAAGTTACGCGGACCAGAGTTCCAGGTTGATGGAACAACCGCCTGGTAGTTGCTGATGATACCGTCTTTAATAACCATCCAGTGAGAGAGCATACCGCGCGGCGCTTCGAGGAAGCCAACACCTTTGAATTCACCCGTTGCCGGAATGTTCGGCTTCACAAAG  >  NC_000913/3141347‑3141619                                                                                                                                         |                                                                                                                                         gCCATGCACGGGTCAAAGGAGTGAATGGTACGCACCACTTCCAGCGGTTTATTCGGATCGGCAACCGGTGTACCCACCAGCGACTGCTCGTAAGGACCGATGTCTTCATTGAAGTTAAGCGGTCCAGAGTTCCAGGCTGa                                                                                                                                       >  2:247623/1‑140 (MQ=255) gCCATGCACGGGTCAAAGGAGTGAATGGTACGCACCACTTCCAGCGGTTTATTCGGATCGGCAACCGGTGTACCCACCAGCGACTGCTCGTAAGGACCGACGACATCATTTAAGCTAAGCGGACAAGAGTTCCAGGCTGa                                                                                                                                       >  1:255722/1‑140 (MQ=255)           ggTTAAAGGAGTGAATGGTACGCACCACTTCCAGCGGTTTATTCGGATCGGCAACCGGTGTACCCACCAGCGACTGCTCGTAAGGACCGACGTCATCATTGAAGTTACGCGGACCAGAGTTCCAGGCTGATGGAACAAcc                                                                                                                             >  1:668200/1‑140 (MQ=255)           ggTCAAAGGAGTGAATGGTACGCACCACTTCCAGCGGTTTATTCGGATCGGCAACCGGTGTACCCACCAGCGACTGCTCGTAAGGACCGACGTCATCATTGAAGTTACGCGGACCAGAGTTCCAGGCTGATGGAACAAcc                                                                                                                             >  2:116671/1‑140 (MQ=255)           ggTCAAAGGAGTGAATGGTACGCACCACTTCCAGCGGTTTATTCGGATCGGCAACCGGTGTACCCACCAGCGACTGCTCGTAAGGACCGACGTCATCATTGAAGTTACGCGGACCAGAGTTCCAGGCTGATGGAACAAcc                                                                                                                             >  2:227464/1‑140 (MQ=255)           ggTCAAAGGAGTGAATGGTACGCACCACTTCCAGCGGTTTATTCGGATCGGCAACCGGTGTACCCACCAGCGACTGCTCGTAAGGACCGACGTCATCATTGAAGTTACGCGGACCAGAGTTCCAGGCTGATGGAACAAcc                                                                                                                             >  2:498520/1‑140 (MQ=255)             tCAAAGGAGTGAATGGTACGCACCACTTCCAGCGGTTTATTCGGATCGGCAACCGGTGTACCCACCAGCGACTGCTCGTAAGGACCGACGTCATCATTGAAGTTACGCGGACCAGAGTTCCAGGCTGATGGAACAACCGc                                                                                                                           <  1:35308/140‑1 (MQ=255)             tCAAAGGAGTGAATGGTACGCACCACTTCCAGCGGTTTATTCGGATCGGCAACCGGTGTACCCACCAGCGACTGCTCGTAAGGACCGACGTCATCATTGAAGTTACGCGGACCAGAGTTCCAGGCTGATGGAACAACCGc                                                                                                                           <  2:540222/140‑1 (MQ=255)                aaGGAGTGAATGGTACGCACCACTTCCAGAGGTTTATACGGATCGGCAACCGGTGTACCCACCAGCGACTGCTCGTAAGGACCGACGTCATCATTGAAGTTACGCGGACCAGAGTTCCAGGCTGATGGAACAACCGCCTg                                                                                                                        <  1:247623/140‑1 (MQ=255)                  ggAGTGAATGGTACGTACCACTACCTGCGATTTATTCGGATCGGCAACCGGTGTACCCACCAGCGACTGCTCGTAAGGACCGACGTCATCATTGAAGTTACGCGGACCAGAGTTCCAGGCTGATGGAACAACCGCCTGGt                                                                                                                      <  1:314906/140‑1 (MQ=255)                    aGTGAATGGTACGCACCACTTCCAGCGGTTTATTCGGATCGGCAACCGGTGTACCCACCAGCGACTGCTCGTAAGGACCGACGTCATCATTGAAGTTACGCGGACCAGAGTTCCAGGCTGATGGAACAACCGCCTGGTAg                                                                                                                    >  1:125062/1‑140 (MQ=255)                    aGTGAATGGTACGCACCACTTCCAGCGGTTTATTCGGATCGGCAACCGGTGTACCCACCAGCGACTGCTCGTAAGGACCGACGTCATCATTGAAGTTACGCGGACCAGAGTTCCAGGCTGATGGAACAACCGCCTGGTAg                                                                                                                    <  2:668200/140‑1 (MQ=255)                      tGAATGGTACGCACCACTTCCAGCGGTTTATTCGGATCGGCAACCGGTGTACCCACCAGCGACTGCTCGTAAGGACCGACGTCATCATTGAAGTTACGCGGACCAGAGTTCCAGGCTGATGGAACAACCGCCTGGTAGtt                                                                                                                  >  2:609801/1‑140 (MQ=255)                      tGAATGGTACGCACCACTTCCAGCGGTTTATTCGGATCGGCAACCGGTGTACCCACCAGCGACTGCTCGTAAGGACCGACGTCATCATTGAAGTTACGCGGACCAGAGTTCCAGGCTGATGGAACAACCGCCTGGTAGtt                                                                                                                  >  2:417041/1‑140 (MQ=255)                      tGAATGGTACGCACCACTTCCAGCGGTTTATTCGGATCGGCAACCGGTGTACCCACCAGCGACTGCTCGTAAGGACCGACGTCATCATTGAAGTTACGCGGACCAGAGTTCCAGGCTGATGGAACAACCGCCTGGTAGtt                                                                                                                  >  2:134018/1‑140 (MQ=255)                       gAATGGTACGCACCACTTCCAGCGGTTTATTCGGATCGGCAACCGGTGTACCCACCAGCGACTGCTCGTAAGGACCGACGTCATCATTGAAGTTACGCGGACCAGAGTTCCAGGCTGATGGAACAACCGCCTGGTAGTTg                                                                                                                 <  2:51481/140‑1 (MQ=255)                             tACGCACCACTTCCAGCGGTTTATTCGGATCGGCAACCGGTGTACCCACCAGCGACTGCTCGTAAGGACCGACGTCATCATTGAAGTTACGCGGACCAGAGTTCCAGGCTGATGGAACAACCGCCTGGTAGTTGCtgatg                                                                                                           >  1:396008/1‑140 (MQ=255)                              aCGCACCACTTCCAGCGGTTTATTCGGATCGGCAACCGGTGTACCCACCAGCGACTGCTCGTAAGGACCGACGTCATCATTGAAGTTACGCTGACCAGAGGTCCGGGCGGATGGAACAACCGGCTGGTAGTTGCtgatga                                                                                                          >  2:183923/1‑140 (MQ=255)                              aCGCACCACTTCCAGCGGTTTATTCGGATCGGCAACCGGTGTACCCACCAGCGACTGCTCGTAAGGACCGACGTCATCATTGAAGTTACGCGGACCAGAGTTCCAGGCTGATGGAACAACCGCCTGGTAGTTGCtgatga                                                                                                          >  1:380257/1‑140 (MQ=255)                                gCACCACTTCCAGCGGTTTATTCGGATCGGCAACCGGTGTACCCACCAGCGACTGCTCGTAAGGACCGACGTCATCATTGAAGTTACGCGGACCAGAGTTCCAGGCTGATGGAACAACCGCCTGGTAGTTGCTGATGATa                                                                                                        <  2:416385/140‑1 (MQ=255)                                 caccacTTCCAGCGGTTTATTCGGATCGGCAACCGGTGTACCCACCAGCGACTGCTCGTAAGGACCGACGTCATCATTGAAGTTACGCGGACCAGAGTTCCAGGCTGATGGAACAACCGCCTGGTAGTTGCTGATGATAc                                                                                                       <  1:606154/140‑1 (MQ=255)                                  accacTTCCAGCGGTTTATTCGGATCGGCAACCGGTGTACCCACCAGCGACTGCTCGTAAGGACCGACGTCATCATTGAAGTTACGCGGACCAGAGTTCCAGGCTGATGGAACAACCGCCTGGTAGTTGCTGATGATAcc                                                                                                      <  2:497506/140‑1 (MQ=255)                                   ccacTTCCAGCGGTTTATTCGGATCGGCAACCGGTGTACCCACCAGCGACTGCTCGTAAGGACCGACGTCATCATTGAAGTTACGCGGACCAGAGTTCCAGGCTGATGGAACAACCGCCTGGTAGTTGCTGATGATACCg                                                                                                     <  1:498520/140‑1 (MQ=255)                                   ccacTTCCAGCGGTTTATTCGGATCGGCAACCGGTGTACCCACCAGCGACTGCTCGTAAGGACCGACGTCATCATTGAAGTTACGCGGACCAGAGTTCCAGGCTGATGGAACAACCGCCTGGTAGTTGCTGATGATACCg                                                                                                     <  1:134018/140‑1 (MQ=255)                                     acTTCCAGCGGTTTATTCGGATCGGCAACCGGTGTACCCACCAGCGACTGCTCGTAAGGACCGACGTCATCATTGAAGTTACGCGGACCAGAGTTCCAGGCTGATGGAACAACCGCCTGGTAGTTGCTGACGATACCGTc                                                                                                   >  2:629465/1‑140 (MQ=255)                                       ttCCAGCGGTTTATTCGGATCGGCAACCGGTGTACCCACCAGCGACTGCTCGTAAGGACCGACGTCATCATTGAAGTTACGCGGACCAGAGTTCCAGGCTGATGGAACAACCGCCTGGTAGTTGCTGATGATACCGTCtt                                                                                                 >  2:549702/1‑140 (MQ=255)                                            gCGGTTTATTCGGATCGGCAACCGGTGTACCCACCAGCGACTGCTCGTAAGGACCGACGTCATCATTGAAGTTACGCGGACCAGAGTTCCAGGCTGATGGAACAACCGCCTGGTAGTTGCTGATGATACCGTCTTtaata                                                                                            >  2:107055/1‑140 (MQ=255)                                                  tATTCGGATCGGCAACCGGTGTACCGACCAGCGACTGCTCGTAAGGACCGACGTCATCATTGAAGTTACGCGGACCAGAGTTCCAGGCTGATGGAACAACCGCCTGGTAGTTGCTGATGATACCGTCTTTAATAACCATc                                                                                      <  1:227464/140‑1 (MQ=255)                                                  tATTCGGATCGGCAACCGGTGTACCCACCAGCGACTGCTCGTAAGGACCGACGTCATCATTGAAGTTACGCGGACCAGAGTTCCAGGCTGATGGAACAACCGCCTGGTAGTTGCTGATGATACCGTCTTTAATAACCATc                                                                                      <  1:116671/140‑1 (MQ=255)                                                    ttCGGATCGGCAACCGGTGTACCCACCAGCGACTGCTCGTAAGGACCGACGTCATCATTGAAGTTACGCGGACCAGAGTTCCAGGCTGATGGAACAACCGCCTGGTAGTTGCTGATGATACCGTCTTTAATAACCATCCa                                                                                    <  1:584942/140‑1 (MQ=255)                                                     tCGGATCGGCAACCGGTGTACCCACCAGCGACTGCTCGTAAGGACCGACGTCATCATTGAAGTTACGCGGACCAGAGTTCCAGGCTGATGGAACAACCGCCTGGTAGTTGCTGATGATACCGTCTTTAATAACCATCCAg                                                                                   <  1:589632/140‑1 (MQ=255)                                                            ggCAACCGGTGTACCCACCAGCGACTGCTCGTAAGGACCGACGTCATCATTGAAGTTACGCGGACCAGAGTTCCAGGCTGATGGAACAACCGCCTGGTAGTTGCTGATGATACCGTCTTTAATAACCATCCAGTgagaga                                                                            >  1:413442/1‑140 (MQ=255)                                                             gCAACCGGTGTACCCACCAGCGACTGCTCGTAAGGACCGACGTCATCATTGAAGTTACGCGGACCAGAGTTCCAGGCTGATGGAACAACCGCCTGGTAGTTGCTGATGATACCGTCTTTAATAACCATCCAGTg                                                                                 >  2:548855/1‑134 (MQ=255)                                                             gCAACCGGTGTACCCACCAGCGACTGCTCGTAAGGACCGACGTCATCATTGAAGTTACGCGGACCAGAGTTCCAGGCTGATGGAACAACCGCCTGGTAGTTGCTGATGATACCGTCTTTAATAACCATCCAGTg                                                                                 <  1:548855/134‑1 (MQ=255)                                                              cAACCGGTGTACCCACCAGCGACTGCTCGTAAGGACCGACGTCATCATTGAAGTTACGCGGACCAGAGTTCCAGGCTGATGGAACAACCGCCTGGTAGTTGCTGATGATACCGTCTTTAATAACCATCCAGTGAGAGAGc                                                                          >  2:63068/1‑140 (MQ=255)                                                                      gtACCCACCAGCGACTGGTCGTAAGGACCGATGTCGTCACTGAAGTTACGCGGACCAGAGTACCAGGCTGATGGAACAACCGCCTGGTAGTTGCTGATGATACCGTCTTTAATAACCATCCAGTGAGAGAGCATACcgcg                                                                  <  2:228221/140‑1 (MQ=255)                                                                        aCCCACCAGCGACTGCTCGTAAGGACCGACGTCATCATTGAAGTTACGCGGACCAGAGTTCCAGGCTGATGGAACAACCGCCTGGTAGTTGCTGATGATACCGTCTTTAATAACCATCCAGTGAGAGAGCATACcgcgcg                                                                >  2:86837/1‑140 (MQ=255)                                                                            accaGCGACTGCTCGTAAGGACCGACGTCATCATTGAAGTTACGCGGACCAGAGTTCCAGGCTGATGGAACAACCGCCTGGTAGTTGCTGATGATACCGTCTTTAATAACCATCCAGTGAGAGAGCATACCGCGCGgcgc                                                            >  2:68879/1‑140 (MQ=255)                                                                            accaGCGACTGCTCGTAAGGACCGACGTCATCATTGAAGTTACGCGGACCAGAGTTCCAGGCTGATGGAACAACCGCCTGGTAGTTGCTGATGATACCGTCTTTAATAACCATCCAGTGAGAGAGCATACCGCGCGgcgc                                                            >  2:568930/1‑140 (MQ=255)                                                                              caGCGACTGCTCGTAAGGACCGACGTCATCATTGAAGTTACGCGGACCAGAATTCCAGGCTGATGGAACAACCGCCTGGTAGTTGCTGATGATACCGTCTTTAATAACCATCCAGTGAGAGAGCATACCGCGCGGCGCtt                                                          >  2:444600/1‑140 (MQ=255)                                                                               aGCGACTGCTCGTAAGGACCGACGTCATCATTGAAGTTACGCGGACCAGAGTTCCAGGCTGATGGAACAACCGCCTGGTAGTTGCTGATGATACCGTCTTTAATAACCATCCAGTGAGAGAGCATACCGCGCGGCGCTTc                                                         >  2:55836/1‑140 (MQ=255)                                                                                          gTAAGGACCGACGTCTTCATTGCAGCTACCCGGACCATAGTTCCAGGCTGATGGAACAACCGCCTGGTAGTTGCTGATGATACCGTCTTTAATAACCATCCAGTGAGAGAGCATACCGCGCGGCGCTTCGAGGAAGCCaa                                              <  1:183923/140‑1 (MQ=255)                                                                                          gTAAGGACCGACGTCATCATTGAAGTTACGCGGACCAGAGTTCCAGGCTGATGGAACAACCGCCTGGTAGTTGCTGATGATACCGTCTTTAATAACCATCCAGTGAGAGAGCATACCGCGCGGCGCTTCGAGGAAGCCaa                                              <  2:380257/140‑1 (MQ=255)                                                                                           tAAGGACCGACGTCATCATTGAAGTTACGCGGACCAGAGTTCCAGGCTGATGGAACAACCGCCTGGTAGTTGCTGATGATACCGTCTTTAAGAACCATCCAGTGCGAGGGCATACCGTGCGGGGCTTCGAGGAAGCCAac                                             >  2:249703/1‑140 (MQ=255)                                                                                               gACCGACGTCATCATTGAAGTTCCGCGGACCAGAGGTCCTGGCTGATGGAACAACCGCCTGGTAGTTGCTGATGATACCGTCTTTAATAACCATCCAGTGAGAGAGCATACCGCGCGGCGCTTCGAGGAAGCCAACACCt                                         <  2:280609/140‑1 (MQ=255)                                                                                               gACCGACGTCATCATTGAAGTTACGCGGACCAGAGTTCCAGGCTGATGGAACAACCGCCTGGTAGTTGCTGATGATACCGTCTTTAATAACCATCCAGTGAGAGAGCATAACGCACGGCGCTTCTAGGAAGCCAACACCt                                         >  1:280609/1‑140 (MQ=255)                                                                                                 ccGACGTCATCATTGAAGTTACGCGGACCAGAGTTCCAGGCTGATGGAACAACCGCCTGGTAGTTGCTGATGATACCGTCTTTAATAACCATCCAGTGAGAGAGCATACCGCGCGGCGCTTCGAGGAAGCCAACACCttt                                       >  2:108207/1‑140 (MQ=255)                                                                                                  cGACGTCATCATTGAAGTTACGCGGACCAGAGTTCCAGGCTGATGGAACAACCGCCTGGTAGTTGCTGATGATACCGTCTTTAATAACCATCCAGTGAGAGAGCATACCGCGCGGCGCTTCGAGGAAGCCAACACCTTTg                                      >  1:150715/1‑140 (MQ=255)                                                                                                    aCGTCATCATTGAAGTTACGCGGACCAGAGTTCCAGGCTGATGGAACAACCGCCTGGTAGTTGCTGATGATACCGTCTTTAATAACCATCCAGTGAGAGAGCATACCGCGCGGCGCTTCGAGGAAGCCAACACCTTTGaa                                    >  1:68033/1‑140 (MQ=255)                                                                                                      gTCATCATTGAAGTTACGCGGACCAGAGTTCCAGGCTGATGGAACAACCGCCTGGTAGTTGCTGATGATACCGTCTTTAATAACCATCCAGTGAGAGAGCATACCGCGCGGCGCTTCGAGGAAGCCAACACCTTTGAAtt                                  <  2:630517/140‑1 (MQ=255)                                                                                                              tGAAGTTACGCGGACCAGAGTTCCAGGCTGATGGAACAACCGCCTGGTAGTTGCTGATGATACCGTCTTTAATAACCATCCAGTGAGAGAGCATACCGCGCGGCGCTTCGAGGAAGCCAACACCTTTGAATTCACCCGtt                          >  1:652131/1‑140 (MQ=255)                                                                                                              tGAAGTTACGCGGACCAGAGTTCCAGGCTGATGGAACAACCGCCTGGTAGTTGCTGATGATACCGTCTTTAATAACCATCCAGTGAGAGAGCATACCGCGCGGCGCTTCGAGGAAGCCAACACCTTTGAATTCACCCGtt                          >  2:613966/1‑140 (MQ=255)                                                                                                                  gTTACGCGGACCAGAGTTCCAGGCTGATGGAACAACCGCCTGGTAGTTGCTGATGATACCGTCTTTAATAACCATCCAGTGAGAGAACATACCGCGCGGCGCGTCGAGGAGGCCAACGCCTTTGAATTCACCCGTTGCCg                      >  1:187606/1‑140 (MQ=255)                                                                                                                  gTTACGCGGACCAGACTTCCAGCCTCATGTAACAACCCCCTGGTAGTTGCTGATGATACCGTCTTTAATAACCATCCAGTGAGAGAGCATACCGCGCGGCGCTTCGAGGAAGCCAACACCTTTGAATTCACCCGTTGCCg                      <  1:183034/140‑1 (MQ=255)                                                                                                                         ggACCAGAGTTCCAGGCTGATGTAACAACCGCCTGGTAGTTGCTGATGATACCGTCTTTAATAACCATCCAGTGAGAGAGCATACCGCGCGGCGCTTCGAGGAAGCCAACACCTTTGAATTCACCCGTTGCCGGAATGtt               <  1:268093/140‑1 (MQ=255)                                                                                                                          gACCAGAGTTCCAGGCTGATGGAACAACCGCCTGGTAGTTGCTGATGATACCGTCTTTAATAACCATCCAGTGAGAGAGCATACCGCGCGGCGCTTCGAGGAAGCCAACACCTTTGAATTCACCCGTTGCCGGAATGTTc              >  1:568621/1‑140 (MQ=255)                                                                                                                           aCCAGAGTTCCAGGCTGATGGAACAACCGCCTGGTAGTTGCTGATGATACCGTCTTTAATAACCATCCAGTGAGAGAGCATACCGCGCGGCGCTTCGAGGAAGCCAACACCTTTGAATTCACCCGTTGCCGGAATGTTCg             >  2:404217/1‑140 (MQ=255)                                                                                                                            ccAGAGTTCCAGGCTGATGGAACAACCGCCTGGTAGTTGCTGATGATACCGTCTTTAATAACCATCCAGTGAGAGAGCATACCGCGCGGCGCTTCGAGGAAGCCAACACCTTTGAATTCACCCGTTGCCGGAATGTTCgg            <  2:592198/140‑1 (MQ=255)                                                                                                                               gagTTCCAGGCTGATGGAACAACCGCCTGGTAGTTGCTGATGATACCGTCTTTAATAACCATCCAGTGAGAGAGCATACCGCGCGGCGCTTCGAGGAAGCCAACACCTTTGAATTCACCCGTTGCCGGAATGTTCGGCtt         <  1:68879/140‑1 (MQ=255)                                                                                                                                 gTTCCAGGCTGATGGAACAACCGCCTGGTAGTTGCTGATGATACCGTCTTTAATAACCATCCAGTGAGAGAGCATACCGCGCGGCGCTTCGAGGAAGCCAACACCTTTGAATTCACCCGTTGCCGGAATGTTCGGCTTca       <  1:613966/140‑1 (MQ=255)                                                                                                                                   tCCAGGCTGATGGAACAACCGCCTGGTAGTTGCTGATGATACCGTCTTTAATAACCATCCAGTGAGAGAGCATACCGCGCGGCGCTTCGAGGAAGCCAACACCTTTGAATTCACCCGTTGCCGGAATGTTCGGCTTcaca     <  1:404217/140‑1 (MQ=255)                                                                                                                                   tCCAGGCTGATGGAACAACCGCCTGGTAGTTGCTGATGATACCGTCTTTAATAACCATCCAGTGAGAGAGCATACCGCGCGGCGCTTCGAGGAAGCCAACACCTTTGAATTCACCCGTTGCCGGAATGTTCGGCTTcaca     <  1:417041/140‑1 (MQ=255)                                                                                                                                   tCCAGGCTGATGGAACAACCGCCTGGTAGTTGCTGATGATACCGTCTTTAATAACCATCCAGTGAGAGAGCATACCGCGCGGCGCTTCGAGGAAGCCAACACCTTTGAATTCACCCGTTGCCGGAATGTTCGGCTTcaca     <  1:55836/140‑1 (MQ=255)                                                                                                                                    ccAGGCTGATGGAACAAGCGCCCGGTAGTTGTTGACGATTCCGTCTTTAATAACCATCCAGTGAGAGAGCATACCGCGCGGCGCTTCGAGGAAGCCAACACCTTTGAATTCACCCGTTGCCGGAATGTTCGGCTTCACaa    <  2:226491/140‑1 (MQ=255)                                                                                                                                    ccAGGCTGATGGAACAACCGCCTGGTAGTTGCTGATGATACCGTCTTTAATAACCATCCAGTGAGAGAGCATACCGCGCGGCGCTTCGAGGAAGCCAACACCTTTGAATTCACCCGTTGCCGGAATGTTCGGCTTCACaa    <  1:662811/140‑1 (MQ=255)                                                                                                                                     cAGGCTGATGGAACAACCGCCTGGTAGTTGCTGATGATACCGTCTTTAATAACCATCCAGTGAGAGAGCATACCGCGCGGCGCTTCGAGGAAGCCAACACCTTTGAATTCACCCGTTGCCGGAATGTTCGGCTTCACaaa   >  2:627081/1‑140 (MQ=255)                                                                                                                                      aGGCTGATGGAACAACCGCCTGGTAGTTGCTGATGATACCGTCTTTAATAACCATCCAGTGAGAGAGCATACCGCGCGGCGCTTCGAGGAAGCCAACACCTTTGAATTCACCCGTTGCCGGAATGTTCGGCTTCACAAAg  <  1:549702/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                         GCCATGCACGGGTCAAAGGAGTGAATGGTACGCACCACTTCCAGCGGTTTATTCGGATCGGCAACCGGTGTACCCACCAGCGACTGCTCGTAAGGACCGACGTCATCATTGAAGTTACGCGGACCAGAGTTCCAGGTTGATGGAACAACCGCCTGGTAGTTGCTGATGATACCGTCTTTAATAACCATCCAGTGAGAGAGCATACCGCGCGGCGCTTCGAGGAAGCCAACACCTTTGAATTCACCCGTTGCCGGAATGTTCGGCTTCACAAAG  >  NC_000913/3141347‑3141619

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑