| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,142,568 | T→C | H141R (CAC→CGC) | hybC ← | hydrogenase 2 large subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,142,568 | 0 | T | C | 97.3% | 122.9 / NA | 37 | H141R (CAC→CGC) | hybC | hydrogenase 2 large subunit |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (17/19); minor base A (0/1); total (17/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01 | |||||||||||
CTTCCGGCGGCAGTTTCATCGCCGGATGACCCCAGTAGCCATTAGCGAAAATACCCAACTGACCGCTGGCAACCAGATCTTTGATCTTGTTCTGAACTTTGGTGAACTCTTCCGGACTGTTCAGGTGCCAGGTCGAAACGCCTTTCAGCATTTCGGAGGCTTTGGTTGGGTCAGCTTGCAGTGCAGAAGTGATGTCCACCCAGTCCAGCGCCGAAAGCTGATAGAAATGAACAATATGGTCATGCGTGGTGTGC > NC_000913/3142443‑3142696 | cTTCCGGCGGCAGTTTCATCGCCGGATGACCCCAGTAGCCATTAGCGAAAATACCCAACTGACCGCTGGCAACCAGATCTTTGATCTTGTTCTGAACTTTGGTGAACTCTTCCGGACTGTTCAGGCGCCAGGTCGAAACg < 2:347956/140‑1 (MQ=255) ttCCGGCGGCAGTTTCATCGCCGGATGACCCCAGTAGCCATTAGCGAAAATACCCAACTGACCGCTGGCAACCAGATCTTTGATCTTGTTCTGAACTTTGGTGAACTCTTCCGGACTGTTCAGGCGCCAGGTCGAAACGc > 1:568114/1‑140 (MQ=255) ccGGCGGCAGTTTCATCGCCGGATGACCCCAGTAGCCATTAGCGAAAATACCCAACTGACCGCTGGCAACCAGATCTTTGATCTTGTTCTGAACTTTGGTGAACTCTTCCGGACTGTTCAGGCGCCAGGTCGAAACGCCt > 1:646603/1‑140 (MQ=255) gcggcAGTTTCATCGCCGGATGACCCCAGTAGCCATTAGCGAAAATACCCAACTGACCGCTGGCAACCAGATCTTTGATCTTGTTCTGAACTTTGGTGAACTCTTCCGGACTGTTCAGGCGCCAGGTCGAAACGCCTTTc > 1:461940/1‑140 (MQ=255) cATCGCCGGATGACCCCAGTAGCCATTAGCGAAAATACCCAACTGACCGCTGGCAACCAGATCTTTGATCTTGTTCTGAACTTTGGTGAACTCTTCCGGACTGTTCAGGCGCCAGGTCGAAACGCCTTTCAGCATTTCgg < 1:613980/140‑1 (MQ=255) ccGGATGACCCCAGTAGCCATTAGCGAAAATACCCAACTGACCGCTGGCAACCAGATCTTTGATCTTGTTCTGAACTTTGGTGAACTCTTCCGGACTGTTCAGGCGCCAGGTCGAAACGCCTTTCAGCATTTCGGAGGCt < 1:651520/140‑1 (MQ=255) cccAGTAGCCATTAGCGAAAATCCCAAACTGACCGCTAGCAACCAGATCTTTGATCTTGTTCTGAACTTTGGTGAACTCTTCCGGACTGTTCAGGCGCCAGGTCGAAACGCCTTTCAGCATTTCGGAGGCTTTGGTTggg < 1:264396/140‑1 (MQ=255) tAGCCATTAGCGAAAATACCCAACTGACCGCTGGCAACCAGATCTTTGATCTTGTTCTGAACTTTGGTGAACTCTTCCGGACTGTTCAGGCGCCAGGTCGAAACGCCTTTCAGCATTTCGGAGGCTTTGGTTGGGTCAGc > 2:155525/1‑140 (MQ=255) ccATTAGCGAAAATACCCAACTGACCGCTGGCAACCAGATCTTTGATCTTGTTCTGAACTTTGGTGAACTCTTCCGGACTGTTCAGGCGCCAGGTCGAAACGCCTTTCAGCATTTCGGAGGCTTTGGTTGGGTCATCTtg < 1:126414/140‑1 (MQ=255) ccATTAGCGAAAATACCCAACTGACCGCTGGCAACCAGATCTTTGATCTTGTTCTGAACTTTGGTGAACTCTTCCGGACTGTTCAGGCGCCAGGTCGAAACGCCTTTCAGCATTTCGGAGGCTTTGGTTGGGTCAGCTtg < 1:475390/140‑1 (MQ=255) aGCGAAAATACCCAACTGACCGCTGGCAACCAGATCTTTGATCTTGTTCTGAACTTTGGTGAACTCTTCCGGACTGTTCAGGCGCCAGGTCGAAACGCCTTTCAGCATTTCGGAGGCTTTGGTTGGGTCAGCTtgcagtg < 1:356453/140‑1 (MQ=255) cGAAAATACCCAACTGACCGCTGGCAACCAGATCTTTGATCTTGTTCTGAACTTTGGTGAACTCTTCCGGACTGTTCAGGCGCCAGGTCGAAACGCCTTTCAGCATTTCGGAGGCTTTGGTTGGGTCAGCTtgcagtgca < 2:528726/140‑1 (MQ=255) cGAAAATACCCAACTGACCGCTGGCAACCAGATCTTTGATCTTGTTCTGAACTTTGGTGAACTCTTCCGGACTGTTCAGGCGCCAGGTCGAAACGCCTTTCAGCATTTCGGAGGCTTTGGTTGGGTCAGCTtgcagtgca > 1:6082/1‑140 (MQ=255) aTACCCAACTGACCGCTGGCAACCAGATCTTTGATCTTGTTCTGAACTTTGGTGAACTCTTCCGGACTGTTCAGGCGCCAGGTCGAAACGCCTTTCAGCATTTCGGAGGCTTTGGTTGGGTCAGCTTGCAGTGCAGAAGt < 1:471199/140‑1 (MQ=255) cTGACCGCTGGCAACCAGATCTTTGATCTTGTTCTGAACTTTGGTGAACTCTTCCGGACTGTTCAGGCGCCAGGTCGAAACGCCTTTCAGCATTTCGGAGGCTTTGGTTGGGTCAGCTTGCAGTGCAGAAGTGATGTCCa > 2:375495/1‑140 (MQ=255) gACCGCTGGCAACCAGATCTTTGATCTTGTTCTGAACTTTGGTGAACTCTTCCGGACTGTTCAGGCGCCAGGTCGAAACGCCTTTCAGCATTTCGGAGGCTTTGGTTGGGTCAGCTTGCAGTGCAGAAGTGATGTCCAcc < 2:6082/140‑1 (MQ=255) aCCGCTGGCAACCAGATCTTTGATCTTGTTCTGAACTTTGGTGAACTCTTCCGGACTGTTCAGGCGCCAGGTCGAAACGCCTTTCAGCATTTCGGAGGCTTTGGTTGGGTCAGCATGAAGTGCAGAAGTGATGTCCAccc > 1:228025/1‑140 (MQ=255) cGCTGGCAACCAGATCTTTGATCTTGTTCTGAACTTTGGTGAACTCTTCCGGACTGTTCAGGCGCCAGGTCGAAACGCCTTTCAGCATTTCGGAGGCTTTGGTTGGGTCAGCTTGCAGTGCAGAAGTGATGTCCACCCAg > 1:374709/1‑140 (MQ=255) aGATCTTTGATCTTGTTCTGAACTTTGGTGAACTCTTCCGGACTGTTCAGGCGCCAGGTCGAAACGCCTTTCAGCATTTCGGAGGCTTTGGTTGGGTCAGCTTGCAGTGCAGAAGTGATGTCCACCCAGTCCAGCGCCGa < 2:461940/140‑1 (MQ=255) tCTTGTTCTGAACTTTGGTGAACTCTTCCGGACTGTTCAGGCGCCAGGTCGAAACGCCTTTCAGCATTTCGGAGGCTTTGGTTGGGTCAGCTTGCAGTGCAGAAGTGATGTCCACCCAGTCCAGCGCCGAAAGCTGATAg < 2:646603/140‑1 (MQ=255) cTTGTTCTGAACTTTGGTGAACTCTTCCGGACTGTTCAGGCGCCAGGTCGAAACGCCTTTCAGCATTTCGGAGGCTTTGGTTGGGTCAGCTTGCAGTGCAGAAGTGATGTCCACCCAGTCCAGCGCCGAAAGCTGATAGa < 2:66813/140‑1 (MQ=255) cTGAACTTTGGTGAACTCTTCCGGACTGTTCAGGCGCCAGGTCGAAACGCCTTTCAGCATTTCGGAGGCTTTGGTTGGGTCAGCTTGCAGTGCAGAAGTGATGTCCACCCAGTCCAGCGCCGAAAGCTGATAGAAATGaa < 2:500847/140‑1 (MQ=255) aaCTTTGGTGAACTCTTCCGGACTGTTCAGGCGCCAGGTCGAAACGCCTTTCAGCATTTCGGAGGCTTTGGTTGGGTCAGCTTGCAGTGCAGAAGTGATGTCCACCCAGTCCAGCGCCGAAAGCTGATAGAAATGAACaa > 2:552645/1‑140 (MQ=255) aCTTTGGTGAACTCTTCCGGACTGTTCAGGCGCCAGGTCGAAACGCCTTTCAGCATTTCGGAGGCTTTGGTTGGGTCAGCTTGCAGTGCAGAAGTGATGTCCACCCAGTCCAGCGCCGAAAGCTGATAGAAATGAACAat < 2:553119/140‑1 (MQ=255) tGGTGAACTCTTCCGGACTGTTCAGGCGCCAGGTCGAAACGCCTTTCAGCATTTCGGAGGCTTTGGTTGGGTCAGCTTGCAGTGCAGAAGTGATGTCCACCCTGTCCAGCGCCGGAAGCTGATACAAATGAACAATATgg > 1:215429/1‑140 (MQ=255) tGGTGAACTCTTCCGGACTGTTCAGGCGCCAGGTCGAAACGCCTTTCAGCATTTCGGAGGCTTTGGTTGGGTCAGCTTGCAGTGCAGAAGTGATGTCCACCCAGTCCAGCGCCGAAAGCTGATAGAAATGaa < 1:101608/132‑1 (MQ=255) tGGTGAACTCTTCCGGACTGTTCAGGCGCCAGGTCGAAACGCCTTTCAGCATTTCGGAGGCTTTGGTTGGGTCAGCTTGCAGTGCAGAAGTGATGTCCACCCAGTCCAGCGCCGAAAGCTGATAGAAATGaa > 2:101608/1‑132 (MQ=255) tGGTGAACTCTTCCGGACTGTTCAGGCGCCAGGTCGAAACGCCTTTCAGCATTTCGGAGGCTTTGGTTGGGTCAGCTTGCAGTGCAGAAGTGATGTCCACCCAGTCCAGCGCCGAAAGCTGATAGAAATGAACAATATgg > 2:413426/1‑140 (MQ=255) tGGTGAACTCTTCCGGACTGTTCAGGCGCCAGGTCGAAACGCCTTTCAGCATTTCGGAGGCTTTGGTTGGGTCAGCTTGCAGTGCAGAAGTGATGTCCACCCAGTCCAGCGCCGAAAGCTGATAGAAATGAACAATATgg > 2:436474/1‑140 (MQ=255) tGGTGAACTCTTCCGGACTGTTCAGGCGCCAGGTCGAAACGCCTTTCAGCATTTCGGAGGCTTTGGTTGGGTCAGCTTGCAGTGCAGAAGTGATGTCCACCCAGTCCAGCGCCGAAAGCTGATAGAAATGAACAATATgg < 1:409280/140‑1 (MQ=255) tGGTGAACTCTTCCGGACTGTTCAGGCGCCAGGTCGAAACGCCTTTCAGCATTTCGGAGGCTTTGGTTGGGTCAGCTTGCAGTGCAGAAGTGATGTCCACCCAGTCCAGCGCCGAAAGCTGATAGAAATGAACAATATgg > 1:401213/1‑140 (MQ=255) ggTGAACTCTTCCGGCCTGTTCTGGAGCCAGGTCGAAGCGCCTTTCAGCATTTCGGAGGCTTTGGTTGGGTCAGCTTGCAGTGCAGAAGTGATGTCCACCCAGTCCAGCGCCGAAAGCTGATAGAAATGAACAATATGGt < 1:299594/140‑1 (MQ=255) ccGGACTGTTCAGGCGCCAGGTCGAAACGCCTTTCAGCATTTCGGAGGCTTTGGTTGGGTCAGCTTGCAGTGCAGAAGTGATGTCCACCCAGTCCAGCGCCGTAAGCTGAAAGAGATAAACAATATGGTCATGCgtggtg > 1:242029/1‑140 (MQ=255) ccGGACTGTTCAGGCGCCAGGTCGAAACGCCTTTCAGCATTTCGGAGGCTTTGGTTGGGTCAGCTTGCAGTGCAGAAGTGATGTCCACCCAGTCCAGCGCCGCAAGCTGATAGACATGAACAATATGGTCATGCgtggtg > 1:266756/1‑140 (MQ=255) gACTGTTCAGGCGCCAGGTCGAAACGCCTTTCAGCATTTCGGAGGCTTTGGTTGGGTCAGCTTGCAGTGCAGAAGTGATGTCCACCCAGTCCAGCGCCGAAAGCTGATAGAAATGAACAATATGGTCATGCGTGGTGTgc < 2:374709/140‑1 (MQ=255) tGTTCAGGCGCCAGGTCGAAACGCCTTTCAGCATTTCGGAGGCTTTGGTTGGGTCAGCTTGCAGTGCAGAAGTGATGTCCACCCAGTCCAGCGCCGAAAGCTGATAGAAATGAACAATATGGt < 2:432339/123‑1 (MQ=255) tGTTCAGGCGCCAGGTCGAAACGCCTTTCAGCATTTCGGAGGCTTTGGTTGGGTCAGCTTGCAGTGCAGAAGTGATGTCCACCCAGTCCAGCGCCGAAAGCTGATAGAAATGAACAATATGGt > 1:432339/1‑123 (MQ=255) | CTTCCGGCGGCAGTTTCATCGCCGGATGACCCCAGTAGCCATTAGCGAAAATACCCAACTGACCGCTGGCAACCAGATCTTTGATCTTGTTCTGAACTTTGGTGAACTCTTCCGGACTGTTCAGGTGCCAGGTCGAAACGCCTTTCAGCATTTCGGAGGCTTTGGTTGGGTCAGCTTGCAGTGCAGAAGTGATGTCCACCCAGTCCAGCGCCGAAAGCTGATAGAAATGAACAATATGGTCATGCGTGGTGTGC > NC_000913/3142443‑3142696 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |