Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,146,549	G→A	R63R (CGC→CGT)	yghW ←	DUF2623 domain‑containing protein YghW

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,146,549	0	G	A	100.0%	141.2 / NA	43	R63R (CGC→CGT)	yghW	DUF2623 domain‑containing protein YghW
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (24/19);  total (24/19)

AGTGTAATTAATTAAAGCAAGATAATACGTATGTTTGATCAATTTTCGAGGCGATAACCGGCCATAAAAAAGCGCAACGTAGAACAGGAATTATTCTCACGAAAGAAATCCATTACCATCTCTTTATCCAGTCCATAGCGGCGCGTCAGAATACCCGCTTCCCAGGCGCTAAGCTGGCGATCTCCGGTCTTTTCGTACATCCGGTGTGAGTAGCCTAATACAAAACCGCGTTTATAATCGGCACAGAATTTTGTCACATTAACCGCACTGTC  >  NC_000913/3146412‑3146683                                                                                                                                          |                                                                                                                                       aGTGTAATTAATTAAAGCAAGATAATACGTATGTTTGATCAATTTTCGAGGCGATAACCGGCCATAAAAAAGCGCAACGTAGAACAGGAATTATTCTCACGAAAGAAATCCATTACCATCTCTTTATCCAGTCCATAacg                                                                                                                                      >  1:43930/1‑140 (MQ=255)  gtgtAATTAATTAAATCAAGATGATGCGTCTGTTTGATCAATTTTCGAGGCGATAACCGGCCATAAAAAAGCGCAACGTAGAACAGGAATTATTCTCACGAAAGAAATCCATTACCATCTCTTTATCCAGTCCATAacgg                                                                                                                                     <  1:232867/140‑1 (MQ=255)  gtgtAATTAATTAAAGCTAGATGATACGTATCTTTCATCCATTTTCGAAGCGATAACCGGCCATAAAAAAGCGCAACGTAGAACAGGAATTATTCTCACGAAAGAAATCCATTACCATCTCTTTATCCAGTCCATAacgg                                                                                                                                     <  2:178549/140‑1 (MQ=255)     taattaatTAAAGCAAGATAATACGTATGTTTGATCAATTTTCGAGGCGATAACCGGCCATAAAAAAGCGCAACGTAGAACAGGAATTATTCTCACGAAAGAAATCCATTACCATCTCTTTATCCAGTCCATAACGgcgc                                                                                                                                  >  2:70297/1‑140 (MQ=255)     taattaatTAAAGCAAGATAATACGTATGTTTGATCAATTTTCGAGGCGATAACCGGCCATAAAAAAGCGCAACGTAGAACAGGAATTATTCTCACGAAAGAAATCCATTACCATCTCTTTATCCAGTCCATAACGgcgc                                                                                                                                  >  1:50536/1‑140 (MQ=255)       attaatt‑aAGCAAGATAATACGTATGTTTGATCAATTTTCGAGGCGATAACCGGCCATAAAAAAGCGCAACGTAGAACAGGAATTATTCTCACGAAAGAAATCCATTACCATCTCTTTATCCAGTCCATAACGGCGCGTc                                                                                                                               <  2:632484/140‑1 (MQ=255)             taaAGCAAGATAATACGTATGTTTGATCAATTTTCGAGGCGATAACCGGCCATAAAAAAGCGCAACGTAGAACAGGAATTATTCTCACGAAGGAAATCCAGTACCATCGCTTTATCCGGTCCGTAACGGCGCGTCAGAAt                                                                                                                          >  2:268481/1‑140 (MQ=255)             taaAGCAAGATAATACGTATGTTTGATCAATTTTCGAGGCGATAACCGGCCATAAAAAAGCGCAACGTAGAACAGGAATTATTCTCACGAAAGAAATCCATTACCATCTCTTTATCCAGTCCATAACGGCGCGTCAGAAt                                                                                                                          >  2:139077/1‑140 (MQ=255)                  cAAGATAATACGTATGTTTGATCAATTTTCGAGGCGATAACCGGCCATAAAAAAGCGCAACGTAGAACAGGAATTATTCTCACGAAAGAAATCCATTACCATCTCTTTATCCAGTCCATAACGGCGCGTCAGAATACCCg                                                                                                                     >  2:23331/1‑140 (MQ=255)                        aataCGTATGTTTGATCAATTTTCGAGGCGATAACCGGCCATAAAAAAGCGCAACGTAGAACAGGAATTATTCTCACGAAAGAAATCCATTACCATCTCTTTATCCAGTCCATAACGGCGCGTCAGAATACCCGCTTccc                                                                                                               <  2:43930/140‑1 (MQ=255)                          taCGTATGTTTGATCAATTTTCGAGGCGATAACCGGCCATAAAAAAGCGCAACGTAGAACAGGAATTATTCTCACGAAAGAAATCCATTACCATCTCTTTATCCAGTCCATAACGGCGCGTCAGAATACCCGCTTCCCAg                                                                                                             >  2:639213/1‑140 (MQ=255)                               aTGTTTGATCAATTTTCGAGGCGATAACCGGCCATAAAAAAGCGCAACGTAGAACAGGAATTATTCTCACGAAAGAAATCCATTACCATCTCTTTATCCAGTGCATAACGACGCATCAGAATACACGCTTCCCAGGCGCt                                                                                                        >  1:319140/1‑140 (MQ=255)                                tGTTTGATCAATTTTCGAGGCGATAACCGGCCATAAAAAAGCGCAACGTAGAACAGGAATTATTCTCACGAAAGAAATCCATTACCATCTCTTTATCCAGTCCATAACGGCGCGTCAGAATACCCGCTTCCCAGGCGCTa                                                                                                       >  2:146295/1‑140 (MQ=255)                                    tGATCAATTTTCGAGGCGATAACCGGCCATAAAAAAGCGCAACGTAGAACAGGAATTATTCTCACGAAAGAAATCCATTACCATCTCTTTATCCAGTCCATAACGGCGCGTCAGAATACCCGCTTCCCAGGCGCTAAGCt                                                                                                   >  1:427266/1‑140 (MQ=255)                                     gATCAATTTTCGAGGCGATAACCGGCCATAAAAAAGCGCAACGTAGAACAGGAATTATTCTCACGAAAGAAATCCATTACCATTTCTTTATCCAGTCCATAACGGCGCGTCAGAATACCCGCTTCCCAGGCGCTAAGCTg                                                                                                  >  1:386033/1‑140 (MQ=255)                                      aTCAATTTTCGAGGCGATAACCGGCCATAAAAAAGCGCAACGTAGAACAGGAATTATTCTCACGAAAGAAATCCATTACCATCTCTTTATCCAGTCCATAACGGCGCGTCAGAATACCCGCTTCCCAGGCGCTAAGCTgg                                                                                                 >  2:82903/1‑140 (MQ=255)                                       tCAATTTTCGAGGCGATAACCGTCCATAAAAAAGCGCAACGTAGAACAGGAATTATTCTCACGAAAGAAATCCATTACCATCTCTTTATCCAGTCCATAACGGCGCGTCAGAATACCCGCTTCCCAGGCGCTAAGCTGGc                                                                                                <  1:273112/140‑1 (MQ=255)                                       tCAATTTTCGAGGCGATAACCGGCCATAAAAAAGCGCAACGTAGAACAGGAATTATTCTCACGAAAGAAATCCATTACCATCTCTTTATCCAGTCCATAACGGCGCGTCAGAATACCCGCTTCCCAGGCGCTAAGCTGGc                                                                                                <  1:640346/140‑1 (MQ=255)                                          aTTTTCGAGGCGATAACCGGCCATAAAAAAGCGCAACGTAGAACAGGAATTATTCTCACGAAAGAAATCCATTACCATCTCTTTATCCAGTCCATAACGGCGCGTCAGAATACCCGCTTCCCAGGCGCTAAGCTGGCGAt                                                                                             <  1:639213/140‑1 (MQ=255)                                            tttCGAGGCGATAACCGCCCATAAAAAAGCGCAACCTAGCACAGGAATCATTCTCACGAAAGAAATCCATTACCATCTCTTTATCCAGTCCATAACGGCGCGTCAGAATACCCGCTTCCCAGGCGCTAAGCTGGCGAtct                                                                                           <  2:178122/140‑1 (MQ=255)                                                    cGATAACCGGCCATAAACAAGCCCAACGTAGCACACGAATTATTCTCATGAAAGAAATCCATTACCATCTCTTTATCCAGTCCATAACGGCGCGTCAGAATACCCGCTTCCCAGGCGCTAAGCTGGCGATCTCCGGTCtt                                                                                   <  2:197276/140‑1 (MQ=255)                                                       tAACCGGCCATAAAAAAGCGCAACGTAGAACAGGAATTATTCTCACGAAAGAAATCCATTACCATCTCTTTATCCAGTCCATAACGGCGCGTCAGAATACCCGCTTCCCAGGCGCTAAGCTGGCGATCTCCGGTCTTTTc                                                                                >  1:640953/1‑140 (MQ=255)                                                        aaCCGGCCATAAAAAAGGTCAAAGTAGAACAGGAATTATGCTCACGAAAGAAATCCATTACCATCTCTTTATCCAGTCCATAACGGCGCGTCAGAATACCCGCTTCCCAGGCGCTAAGCTGGCGATCTCCGGACTTTTCg                                                                               <  2:235973/140‑1 (MQ=255)                                                        aaCCGGCCATAAAAAAGCGCAACGTAGAACAGGAATTATTCTCACGAAAGAAATCCATTACCATCTCTTTATCCAGTCCATAACGGCGCGTCAGAATACCCGCTTCCCAGGCGCTAAGCTGGCGATCTCCGGTCTTTTCg                                                                               <  2:114400/140‑1 (MQ=255)                                                             gCCATAAAAAAGCGCAACGTAGAACAGGAATTATTCTCACGAAAGAAATCCATTACCATCTCTTTATCCAGTCCATAACGGCGCGTCAGAATACCCGCTTCCCAGGCGCTAAGCTGGCGATCTCCGGTCTTTTCGTACAt                                                                          >  1:50599/1‑140 (MQ=255)                                                                  aaaaaaGCGCAACGTAGAACAGGAATTATTCTCACGAAAGAAATCCATTACCATCTCTTTATCCAGTCCATAACGGCGCGTCAGAATACCCGCTTCCCAGGCGCTAAGCTGGCGATCTCCGGTCTTTTCGTACATCCGgt                                                                     <  2:396742/140‑1 (MQ=255)                                                                     aaaGCGCAACGTAGAACAGGAATTATTCTCACGAAAGAAATCCATTACCATCTCTTTATCCAGTCCATAACGGCGCGTCAGAATACCCGCTTCCCAGGCGCTAAGCTGGCGATCTCCGGTCTTTTCGTACATCCGGtgtg                                                                  >  1:72931/1‑140 (MQ=255)                                                                       aGCGCAACGTAGAACAGTAATTTTTCTCACGAAAGCAATTCATTACCACCTCTTTATCCAGTCCATAACGGCGCGTCAGAATACCCGCTTCCCAGGCGCTAAGCTGGCGATCTCCGGTCTTTTCGTACATCCGGTGTGAg                                                                <  2:266141/140‑1 (MQ=255)                                                                       aGCGCAACGTAGAACAGGAATTATTCTCACGAAAGAAATCCATTACCATCTCTTTATCCAGTCCATAACGGCGCGTCAGAATACCCGCTTCCCAGGCGCTAAGCTGGCGATCTCCGGTCTTTTCGTACATCCGGTGTGAg                                                                <  2:427266/140‑1 (MQ=255)                                                                       aGCGCAACGTAGAACAGGAATTATTCTCACGAAAGAAATCCATTACCATCTCTTTATCCAGTCCATAACGGCGCGTCAGAATACCCGCTTCCCAGGCGCTAAGCTGGCGATCTCCGGTCTTTTCGTACATCCGGTGTGAg                                                                <  2:339334/140‑1 (MQ=255)                                                                            aaCGTAGAACAGGAATTATTCTCACGAAAGAAATCCATTACCATCTCTTTATCCAGTCCATAACGGCGCGTCAGAATACCCGCTTCCCAGGCGCTAAGCTGGCGATCTCCGGTCTTTTCGTACATCCGGTGTGAGTAGcc                                                           <  2:50536/140‑1 (MQ=255)                                                                             aCGTAGAACAGGAATTATTCTCACGAAAGAAATCCATTACCATCTCTTTATCCAGTCCATAACGGCGCGTCAGAATACCCGCTTCCCAGGCGCTAAGCTGGCGATCTCCGGTCTTTTCGTACATCCGGTGTGAGTAGCCt                                                          <  1:70297/140‑1 (MQ=255)                                                                                        gAATTATTCTCACGAAAGAAATCCATTACCATCTCTTTATCCAGTCCATAACGGCGCGTCAGAATACCCGCTTCCCAGGCGCTAAGCTGGCGATCTCCGGTCTTTTCGTACATCCGGTGTGAGTAGCCTAATACAAAAcc                                               >  1:490808/1‑140 (MQ=255)                                                                                                  cACGAAAGAAATCCATTACCATCTCTTTATCCAGTCCATAACGGCGCGTCAGAATACCCGCTTCCCAGGCGCTAAGCTGGCGATCTCCGGTCTTTTCGTACATCCGGTGTGAGTAGCCTAATACAAAACCGCGTTTATaa                                     >  2:620781/1‑140 (MQ=255)                                                                                                   aCGAAAGAAATCCATTACCATCTCTTTATCCAGTCCATAACGGCGCGTCAGAATACCCGCTTCCCAGGCGCTAAGCTGGCGATCTCCGGTCTTTTCGTACATCCGGTGTGAGTAGCCTAATACAAAACCGCGTTTATAAt                                    <  2:490808/140‑1 (MQ=255)                                                                                                   aCGAAAGAAATCCATTACCATCTCTTTATCCAGTCCATAACGGCGCGTCAGAATACCCGCTTCCCAGGCGCTAAGCTGGCGATCTCCGGTCTTTTCGTACATCCGGTGTGAGTAGCCTAATACAAAACCGCGTTTATAAt                                    <  2:50599/140‑1 (MQ=255)                                                                                                                       tctcTTTATCCAGTCCATAACGGCGCGTCAGAATACCCGCTTCCCAGGCGCTAAGCTGGCGATCTCCGGTCTTTTCGTACATCCGGTGTGAGTAGCCTAATACAAAACCGCGTTTATAATCGGCACAGAATTTTGTcaca                >  1:592781/1‑140 (MQ=255)                                                                                                                              aTCCAGTCCATAACGGCGCGTCAGAATACCCGCTTCCCAGGCGCTAAGCTGGCGATCTCCGGTCTTTTCGTACATCCGGTGTGAGTAGCCTAATACAAAACCGCGTTTATAATCGGCACAGAATTTTGTCACATTAACCg         <  2:516628/140‑1 (MQ=255)                                                                                                                               tCCAGTCCATAACGGCGCGTCAGAATACCCGCTTCCCAGGCGCTAAGCTGGCGATCTCCGGTCTTTTCGTACATCCGGTGTGAGTAGCCTAATACAAAACCGCGTTTATAATCGGCACAGAATTTTGTCACATTAACCGc        >  2:573209/1‑140 (MQ=255)                                                                                                                                   gTCCATAACGGCGCGTCAGAATACCCGCTTCCCAGGCGCTAAGCTGGCGATCTCCGGTCTTTTCGTACATCCGGTGTGAGTAGCCTAATACAAAACCGCGTTTATAATCGGCACGGAATTTTGTTACATTAACCGCACTg    >  1:239192/1‑140 (MQ=255)                                                                                                                                   gTCCATAACGGCGCGTCAGAATACCCGCTTCCCAGGCGCTAAGCTGGCGATCTCCGGTCTTTTCGTACATCCGGTGTGAGTAGCCTAATACAAAACCGCGTTTATAATCGCCACGGAATTTTGTCACATTAACCGCACTg    >  1:227430/1‑140 (MQ=255)                                                                                                                                   gTCCATAACGGCGCGTAGGAATACCCGCTTCTCAGACGCCAAGCTGGCAATCTCCGGTCTTTTCGTACATCCGGTGTGAGTAGCCTAATACAAAACCGCGTTTATAATCGGCACAGAATTTTGTCACATTAACCGCACTg    <  2:322428/140‑1 (MQ=255)                                                                                                                                    tCCATAACGGCGCGTCAGAATACCCGCTTCCCAGGCGCTAAGCTGGCGATCTCCGGTCTTTTCGTACATCCGGTGTGAGTAGCCTAATACAAAACCGCGTTTATAATCGGCACAGAATTTTGTCACATTAACCGCACTGt   >  1:643793/1‑140 (MQ=255)                                                                                                                                    tCCATAACGGCGCGTCAGAATACCCGCTTCCCAGGCGCTAAGCTGGCGATCTCCGGTCTTTTCGTACATCCGGTGTGAGTAGCCTAATACAAAACCGCGTTTATAATCGGCACAGAATTTTGTCACATTAACCGCACTGt   >  2:609090/1‑140 (MQ=255)                                                                                                                                    tCCATAACGGCGCGTCAGAATACCCGCTTCCCAGGCGCTAAGCTGGCGATCTCCGGTCTTTTCGTACATCCGGTGTGAGTAGCCTAATACAAAACCGCGTTTATAATCGGCACAGAATTTTGTCACATTAACCGCACTGt   >  1:148774/1‑140 (MQ=255)                                                                                                                                     ccATAACGGCGCGTCAGAATACCCGCTTCCCAGGCGCTAAGCTGGCGATCTCCGGTCTTTTCGTACATCCGGTGTGAGTAGCCTAATACAAAACCGCGTTTATAATCGGCACAGAATTTTGTCACATTAACCGCACTGTc  >  1:119452/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                       AGTGTAATTAATTAAAGCAAGATAATACGTATGTTTGATCAATTTTCGAGGCGATAACCGGCCATAAAAAAGCGCAACGTAGAACAGGAATTATTCTCACGAAAGAAATCCATTACCATCTCTTTATCCAGTCCATAGCGGCGCGTCAGAATACCCGCTTCCCAGGCGCTAAGCTGGCGATCTCCGGTCTTTTCGTACATCCGGTGTGAGTAGCCTAATACAAAACCGCGTTTATAATCGGCACAGAATTTTGTCACATTAACCGCACTGTC  >  NC_000913/3146412‑3146683

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑