Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,208,251	G→A	P76P (CCG→CCA)	ttdT →	L‑tartrate:succinate antiporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,208,251	0	G	A	100.0%	86.1 / NA	26	P76P (CCG→CCA)	ttdT	L‑tartrate:succinate antiporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (19/7);  total (19/7)

AACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATCCCTCTCGTGGGCCGTTTCCGGTTTTTCTAATTCGGTTATCTGGCTGATTTTCGCCGCCTTTATGTTTGGCACAGGCTATGAAAAAACCGGGCTT  >  NC_000913/3208114‑3208386                                                                                                                                          |                                                                                                                                        aaCCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGATATATCCAGCATAGCCATACTCTCTACCTGACTGCTGTTCAGCCCAGa                                                                                                                                       >  2:183383/1‑140 (MQ=255)             ctctACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCAGAGCAGCTCGCTCa                                                                                                                           >  2:538528/1‑140 (MQ=255)                 aCTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCACTGGCTGGTGTGCAGCCCAGACCAGCTCGCTCAGCCa                                                                                                                       >  1:187979/1‑140 (MQ=255)                          tttttACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCAGAGCAGCTCGCTCAGCCAGGCTTTaaa                                                                                                              >  1:134809/1‑140 (MQ=255)                                    cGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCAGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCaa                                                                                                    >  2:551562/1‑140 (MQ=255)                                      tGATCGTTGGACTGATCTTCGACCCCGTGCCCGGTGCCGAGGTGGCGAGGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCAGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCaaaa                                                                                                  <  2:187979/140‑1 (MQ=255)                                                   gATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCAGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATCCCTCTCGTggg                                                                                     <  1:483121/140‑1 (MQ=255)                                                    aTCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCAGAGCAGCTCGCTCCGCCGGGCTTTAAATTCTCTGCGAAATCCCTCTCGTGGGc                                                                                    >  2:195889/1‑140 (MQ=255)                                                            aCCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCAGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATCCCTCTCGTGGGCCGTTTCCg                                                                            <  1:60347/140‑1 (MQ=255)                                                                         gCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCAGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATCCCTCTCGTGGGCCGTTTCCGGTTTTTCTAATTc                                                               <  1:160001/140‑1 (MQ=255)                                                                                 ggCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCAGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATCCCTCTCGTGGGCCGTTTCCGGTTTTTCTAATTCGGTTATCt                                                       >  2:649723/1‑140 (MQ=255)                                                                                                  ccATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCAGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATCCCTCTCGTGGGCCGTTTCCGGTTTTTCTAATTCGGTTATCTGGCTGATTTTCgccgcc                                      >  1:412444/1‑140 (MQ=255)                                                                                                   catcatCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCAGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATCCCTCTCGTGGACCGTTTCCAGTTCTTCAAATTCGGTTATCTGGCTGATTTTCGCCGCCt                                     >  2:252019/1‑140 (MQ=255)                                                                                                     tcatcGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCAGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATCCCTCTCGTGGGCCGTTTCCGGTTTTTCTAATTCGGTTATCTGGCTGATTTTCGCCGCCttt                                   >  1:63834/1‑140 (MQ=255)                                                                                                     tcatcGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCAGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATCCCTCTCGTGGGCCGTTTCCGGTTTTTCTAATTCGGTTATCTGGCTGATTTTCGCCGCCttt                                   >  2:163967/1‑140 (MQ=255)                                                                                                       atcGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCAGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATCCCTCTCGTGGGCCGTTTCCGGTTTTTCTAATTCGGTTATCTGGCTGATTTTCGCCGCCTTTAt                                 >  2:472290/1‑140 (MQ=255)                                                                                                         cGCCATACTCTCTCCTTGGCTGATGCTCAGCCCAGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATCCCTCTCGTGGGCCGTTTCCGGTTTTTCTAATTCGGTTATCTGGCTGATTTTCGCCGCCTTTATGt                               <  1:183383/140‑1 (MQ=255)                                                                                                         cGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCAGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATCCCTCTCGTGGGCCGTTTCCGGTTTTTCTAATTCGGTTATCTGGCTGATTTTCGCCGCCTTTATGt                               <  1:391273/140‑1 (MQ=255)                                                                                                           ccATACTCTCTCCCTAGCTGCTGTTCAGCCCAGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATCCCTCTCGTGGGCCGTTTCCGGTTTTTCTAATTCGGTTATCTGGCTGATTTTCGCCGCCTTTATGttt                             >  1:112387/1‑140 (MQ=255)                                                                                                               aCTCTCTCCCTGGCTGCTGTTCAGCCCAGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATCCCTCTCGTGGGCCGTTTCCGGTTTTTCTAATTCGGTTATCTGGCTGATTTTCGCCGCCTTTATGTTTGGca                         <  1:163967/140‑1 (MQ=255)                                                                                                                    ctcCCTGGCTGCTGTTCAGCCCAGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATCCCTCTCGTGGGCCGTTTCCGGTTTTTCTAATTCGGTTATCTGGCTGATTTTCGCCGCCTTTATGTTTGGCACAGGc                    >  2:532858/1‑140 (MQ=255)                                                                                                                             tgctgTTCAGCCCAGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATCCCTCTCGTGGGCCGTTTCCGGTTTTTCTAATTCGGTTATCTGGCTGAGTTTCGCCCCCTGTAGGTTTGGCACAGGCTATGaaaaa           >  1:242507/1‑140 (MQ=255)                                                                                                                               ctgTTCAGCCCAGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATCCCTCTCGTGGGCCGTTTCCGGTTTTTCTAATTCTGTTATCTGGCTGATTTTCGCCGCCTTTATGTTTGGCACAGGCTATGAAAAAAc         >  2:512596/1‑140 (MQ=255)                                                                                                                               ctgTTCAGCCCAGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATCCCTCTCGTGGGCCGTTTCCGGTTTTTCTAATTCGGTTATCTGGCTGATTTTCGCCGCCTTTATGTTTGGCACAGGCTATGAAAAAAc         >  1:392562/1‑140 (MQ=255)                                                                                                                               ctgTTCAGCCCAGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATCCCTCTCGTGGGCCGTTTCCGGTTTTTCTAATTCGGTTATCTGGCTGATTTTCGCCGCCTTTATGTTTGGCACAGGCTATGAAAAAAc         >  1:30404/1‑140 (MQ=255)                                                                                                                                      gCCCAGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATCCCTCTCGTGGGCCGTTTCCGGTTTTTCTAATTCGGTTATCTGGCTCATTTTCGCTGCCGTTATGTTTGGCAAAGGCTATGAAAAAACCGGGCtt  >  2:275833/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                        AACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATCCCTCTCGTGGGCCGTTTCCGGTTTTTCTAATTCGGTTATCTGGCTGATTTTCGCCGCCTTTATGTTTGGCACAGGCTATGAAAAAACCGGGCTT  >  NC_000913/3208114‑3208386

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑