Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,232,156	C→T	S164S (TCC→TCT)	fadH →	2,4‑dienoyl‑CoA reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,232,156	0	C	T	100.0%	75.9 / NA	23	S164S (TCC→TCT)	fadH	2,4‑dienoyl‑CoA reductase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (11/12);  total (11/12)

CATTGCAGGCCCCCATCAACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCAA  >  NC_000913/3232020‑3232287                                                                                                                                         |                                                                                                                                    cATTGCAGGCCCCCATCAACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCTGaa                                                                                                                                  <  2:491354/140‑1 (MQ=255)              cATCAACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCTGAAGGGTATTTGATCa                                                                                                                     >  1:469141/1‑140 (MQ=255)                   aCCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCTGAAGGGTATTTGATCAACGaa                                                                                                                >  2:40094/1‑140 (MQ=255)                       tttCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCTGAAGGGTATTTGATCAACGAATTTc                                                                                                            >  2:494064/1‑140 (MQ=255)                           gcgCCCCATGAGTTAAGCCCTGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGGAACGACGGTGCAGAGGAGATGGGTTCTGCAGGGTATTTGATCAACGAATTTCTGAc                                                                                                        >  2:660174/3‑140 (MQ=255)                            tGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCTGAAGGGTATTTGATCAACGAATTTCTGACg                                                                                                       <  2:156599/140‑1 (MQ=255)                              ccccATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCTGAAGGGTATTTGATCAACGAATTTCTGACGCt                                                                                                     <  1:494064/140‑1 (MQ=255)                                     aGTTAAGCCATGAAGAGATCCTCCATCTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCTGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCAcc                                                                                              <  1:207946/140‑1 (MQ=255)                                         aaGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGAGTTCTGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATc                                                                                          >  2:121945/1‑140 (MQ=255)                                          aGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCTGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCa                                                                                         <  1:381184/140‑1 (MQ=255)                                               tGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGAGATGGGTTCTGAAGGGGATTTGATCAACGTATTACTGACGCTGAGCACCAATCAGCGTa                                                                                    >  1:222534/1‑140 (MQ=255)                                                gAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCTGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCa                                                                                         >  2:393911/1‑134 (MQ=255)                                                gAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCTGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCa                                                                                         <  1:393911/134‑1 (MQ=255)                                                 aaGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCTGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGt                                                                                  <  2:535117/140‑1 (MQ=255)                                                 aaGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCTGAAGGGTATTTAATCAACGTATTTCTGAGGCTGCGCACCAATCAGCGTAGt                                                                                  >  2:270365/1‑140 (MQ=255)                                                 aaGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCAGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCTGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGt                                                                                  <  1:384334/140‑1 (MQ=255)                                                                                    tGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGCGATGGGTTCTGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGTCTAGTGGGGCGGCGATTACCGCAACCGGATGCg                                               >  2:395206/1‑140 (MQ=255)                                                                                      cgcgcgACTGGCGCGGTAGGCATGATACGACTGTGTAGGTGTGATGGGATCTGAAGGGTATTTGATCAACGAATTTCTGACACTGCGCTTCAATCAGCGTAGTGAACAGTGGGGCGGCGATTACCGCAACCGGATGCGAt                                             <  2:222534/140‑1 (MQ=255)                                                                                          cAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCTGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGc                                         <  2:461333/140‑1 (MQ=255)                                                                                                   cgGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCTGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAagt                                <  1:40094/140‑1 (MQ=255)                                                                                                           aGGATACGACGGTGTTGAGGTGTTGGGTTTTGAAGGGAATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAgtgcgtg                        <  1:270365/140‑1 (MQ=255)                                                                                                             gATACGACGGTGTAGAGGTGATGGGTTCTGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGtgcgtgcg                      >  2:500369/1‑140 (MQ=255)                                                                                                                       tgtAGAGGTGATGGGTTCTGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAAc            >  2:658765/1‑140 (MQ=255)                                                                                                                       tgtAGAGGTGATGGGTTCTGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGAGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAAc            >  2:153564/1‑140 (MQ=255)                                                                                                                                 aTGGGTTCTGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCaa  >  2:546861/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                    CATTGCAGGCCCCCATCAACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCAA  >  NC_000913/3232020‑3232287

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑