Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,252,476 T→C I58T (ATC→ACC)  yhaH → putative inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,252,4760TC100.0% 169.1 / NA 48I58T (ATC→ACC) yhaHputative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (29/19);  total (29/19)

GTTTCCGGGGTCGTGCGCGGCGCAAAGAGTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAACT  >  NC_000913/3252341‑3252610
                                                                                                                                       |                                                                                                                                      
gTTTCCGGGGTCGTGCGCGGCGCAAAGAGTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAACCTAc                                                                                                                                    >  2:98698/1‑140 (MQ=255)
               cgcgGCGCAAAGAGTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAACCTACGGTATTCTGGTGttt                                                                                                                     >  2:415645/1‑140 (MQ=255)
                  ggcgCAAAGAGTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAACCTACGGTATTCTGGTGTTTTTa                                                                                                                  <  2:576963/140‑1 (MQ=255)
                    cgcAAAGAGTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAACCTACGGTATTCTGGTGTTTTTAcc                                                                                                                >  2:84480/1‑140 (MQ=255)
                    cgcAAAGAGTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAACCTACGGTATTCTGGTGTTTTTAcc                                                                                                                >  1:454314/1‑140 (MQ=255)
                              aCTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAACCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCg                                                                                                      >  2:589959/1‑140 (MQ=255)
                                   aggTTTATTCTGGTCTACATCAGCTTTACCTTCGTGCAGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAACCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCa                                                                                                 <  1:197690/138‑1 (MQ=255)
                                     gTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAACCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGt                                                                                               >  1:18373/1‑140 (MQ=255)
                                     gTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAACCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGt                                                                                               >  2:19753/1‑140 (MQ=255)
                                       ttATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGTATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAACCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTc                                                                                             >  2:664011/1‑140 (MQ=255)
                                       ttATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAACCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTc                                                                                             >  2:494383/1‑140 (MQ=255)
                                       ttATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAACCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTc                                                                                             >  1:68272/1‑140 (MQ=255)
                                       ttATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAACCTACGGTATACTGGTGTTTTTACCGCTGTGGGCGTTTCAGTTc                                                                                             >  2:310533/1‑140 (MQ=255)
                                         aTTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAACCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTccg                                                                                           >  2:503857/1‑140 (MQ=255)
                                          ttCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAACCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTccgc                                                                                          >  1:138699/1‑140 (MQ=255)
                                               gTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAACCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCt                                                                                     >  2:668192/1‑140 (MQ=255)
                                                tCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAACCTAGGGTATTCTTGTGTTTTTACCGTGGTTGGCGGTTCAGTTCCgccgccct                                                                                    >  2:197690/1‑138 (MQ=255)
                                                tCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAACCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTg                                                                                    >  1:616600/1‑140 (MQ=255)
                                                tCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAACCTACGGTATTCTGGTGATTTCACCGTGGTCGGCGATTCAGTTCCGCCGCCTg                                                                                    >  2:240677/1‑140 (MQ=255)
                                                  aaCATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAACCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCa                                                                                  <  2:369362/140‑1 (MQ=255)
                                                    catcatCTTTACGTTCGTGCTGGGACTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAACCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACg                                                                                >  1:428016/1‑140 (MQ=255)
                                                              aCGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAACCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACaccgaccg                                                                      >  1:31070/1‑140 (MQ=255)
                                                                  tCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAACCTACGGTATTCTGGTGTTTTTACCGTGGTGGGAGGTTCAGATCCGCCGCCTGCAGGACACCGACCGCTCg                                                                  >  1:181614/1‑140 (MQ=255)
                                                                    gTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAACCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGc                                                                >  1:409483/1‑140 (MQ=255)
                                                                     tGCTGGGGCTACTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAACCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCAGCCACCGGCACGACACCGACCGCTCGGCg                                                               >  1:294166/1‑140 (MQ=255)
                                                                       cTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAACCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCgtg                                                             <  1:384917/140‑1 (MQ=255)
                                                                         ggggCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAACCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCgtggt                                                           <  1:401371/140‑1 (MQ=255)
                                                                         ggggCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAACCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCgtggt                                                           <  1:78903/140‑1 (MQ=255)
                                                                          gggCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAACCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCgtggtg                                                          >  1:30412/1‑140 (MQ=255)
                                                                            gctgctGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAACCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTggg                                                        <  2:18373/140‑1 (MQ=255)
                                                                            gctgctGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAACCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTggg                                                        <  1:415645/140‑1 (MQ=255)
                                                                             ctgctgGATAAAATGTTAGGCTGGCAACGGGCTGGTGACGAAGGCATCCTGACGACAACCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGc                                                       <  1:303582/140‑1 (MQ=255)
                                                                                ctgGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAACCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACt                                                    >  1:114514/1‑140 (MQ=255)
                                                                                             tAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAACCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCAACCGCTCGGCGTGGTGGCCACTGCTATTCTTAATc                                       >  2:254349/1‑140 (MQ=255)
                                                                                             tAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAACCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACCACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATc                                       >  2:370651/1‑140 (MQ=255)
                                                                                              aGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAACCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATcc                                      <  1:503857/140‑1 (MQ=255)
                                                                                                     cAACGCGCTGGTGGCGAAGGCATCCTGACGACAACCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCCGGGTGGTGGGCACTGCTCTTCTTAATACCGTTTAt                               >  2:310868/1‑140 (MQ=255)
                                                                                                      aaCGCGCTGGTGGCGTAGGCATCCTTACGTCAACCTAGGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATc                              <  1:240677/140‑1 (MQ=255)
                                                                                                       aCGCGCTGGTGGCGAAGGCATCCTGACGACAACCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACCACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCg                             <  1:370651/140‑1 (MQ=255)
                                                                                                        cgcgCTGGTGGCGAATGCATCCTGATGACCACCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCgg                            <  1:310533/140‑1 (MQ=255)
                                                                                                        cgcgCTGGTGGCGAAGGCATCCTGACGACAACCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCgg                            <  2:563623/140‑1 (MQ=255)
                                                                                                          cgcTGGTGGCGAAGGCATCCTGACGACAACCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCggct                          <  1:589959/140‑1 (MQ=255)
                                                                                                            cTGGTGGCGAAGGCATCCTGACGACAACCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCCATTCTTAATCCCGTTTATCggctgg                        <  2:454314/140‑1 (MQ=255)
                                                                                                             tggtggCGAAGGCATCCTGACGACAACCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCggctggc                       <  2:138699/140‑1 (MQ=255)
                                                                                                              ggtggCGAAGGCATCCTGACGACAACCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCggctggct                      <  1:668192/140‑1 (MQ=255)
                                                                                                                     aaGGCATCCTGACGACAACCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTatc               >  1:432964/1‑140 (MQ=255)
                                                                                                                          aTCCTGACGACAACCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGt          <  1:84480/140‑1 (MQ=255)
                                                                                                                                  gacAACCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAACt  >  2:521143/1‑140 (MQ=255)
                                                                                                                                       |                                                                                                                                      
GTTTCCGGGGTCGTGCGCGGCGCAAAGAGTACTGGATGTTTATTCTGGTCAACATCATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAACT  >  NC_000913/3252341‑3252610

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑