| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,272,461 | T→C | A97A (GCA→GCG) | garR ← | tartronate semialdehyde reductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,272,461 | 0 | T | C | 97.3% | 120.6 / NA | 37 | A97A (GCA→GCG) | garR | tartronate semialdehyde reductase |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (16/20); minor base A (1/0); total (17/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.59e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AAATAGCCTTGTCGCCGCCCACCATCACTGACAGCGTACCGTCGATGGCTTTCGGTTCACCGCCGCTCACCGGAGCATCCAGCATATCAATGCCTTTCGCTTTCAGCGCTTCGCTGATTTCACGGCTTGCCAGCGGTGCGATAGAACTCATATCGATCAATACCGTACCTGGCTTCGCGCCTTCAATAATGCCATTCTCACCCAGCGCCACCTCTTTCACATGAGGGGAGTTTGGCAGCATGGTTATGATGACGTCGCA > NC_000913/3272325‑3272583 | aaaTAGCCTTGTCGCCGCCCACCATCACTGACAGCGTACCGTCGATGGCTTTCGGTTCACCGCCGCTCACCGGAGCATCCAGCATATCAATGCCTTTCGCTTTCTGCGGTTCGCTGATTTCATGGCTTGCCAGCGGcgcg > 2:203955/1‑140 (MQ=255) aaTAGCCTTGTCGCCCCCCACCATCTCTGGCAGCGTACCGTCGATGGCTTTCGGTTCACCGCCGCTCACCGGAGCATCCAGCATATCAATGCCTTTCGCTTTCAGCGCTTCGCTGATTTCACGGCTTGCCAGCGGCGCGa < 1:236331/140‑1 (MQ=255) aTAGCCTTGTCGCCGCCCACCATCACTGACAGCGTCCCGTCGATGGCTTTCGGTTCACCGCCGCTCACCGGAGCATCCAGCATATCAATGCCTTTCGCTTTCAGCGCTTCGCTGATTTCACGGCTTGCCAGCGGCGCGAt < 1:576527/140‑1 (MQ=255) aTAGCCTTGTCGCCGCCCACCATCACTGACAGCGTACCGTCGATGGCTTTCGGTTCACCGCCGCTCACCGGAGCATCCAGCATATCAATGCCTTTCGCTTTCAGCGCTTCGCTGATTTCACGGCTTGCCAGCGGCGCGAt < 1:665524/140‑1 (MQ=255) aTAGCCTTGTCGCCGCCCACCATCACTGACAGCGTACCGTCGATGGCTTTCGGTTCACCGCCGCTCACCGGAGCATCCAGCATATCAATGCCTTTCGCTTTCAGCGCTTCGCTGATTTCACGGCTTGCCAGCGGCGCGAt < 1:360098/140‑1 (MQ=255) gccgccCACCATCACTGACAGCGTACCGTCGATGGCTTTCGGTTCACCGCCGCTCACCGGAGCATCCAGCATATCAATGCCTTTCGCTTTCAGCGCTTCGCTGATTTCACGGCTTGCCAGCGGCGCGATAGAACTCatat < 1:656221/140‑1 (MQ=255) gccCACCATCACTGACAGCGTACCGTCGATGGCTTTCGGTTCACCGCCGCTCACCGGAGCATCCAGCATATCAATGCCTTTCGCTTTCAGCGCTTCGCTGATTTCACGGCTTGCCAGCGGCGCGATAGAACTCATATCGa > 2:644750/1‑140 (MQ=255) ccaccaTCACTGACAGCGTACCGTCGATGGCTTTCGGTTCACCGCCGCTCACCGGAGCATCCAGCATATCAATGCCTTTCGCTTTCAGCGCTTCGCTGATTTCACGGCTTGCCAGCGGCGCGATAGAACTCATATCGATc < 2:430073/140‑1 (MQ=255) caTCACTGACAGCGTACCGTCGATGGCTTTCGGTTCACCGCCGCTCACCGGAGCATCCAGCATATCAATGCCTTTCGCTTTCAGCGCTTCGCTGATTTCACGGCTTGCCAGCGGCGCGATAGAACTCATATCGATCAATa < 1:7284/140‑1 (MQ=255) cTGACAGCGTACCGTCGATGGCTTTCGGTTCACCGCCGCTCACCGGAGCATCCAGCATATCAATGCCTTTCGCTTTCAGCGCTTCGCTGATTTCACGGCTTGCCAGCGGCGCGATAGAACTCATATCGATCAATACCGTa > 2:365391/1‑140 (MQ=255) aGCGTACCGTCGATGGCTTTCGGTTCACCGCCGCTCACCGGAGCATCCAGCATATCAATGCCTTTCGCTTTCAGCGCTTCGCTGATTTCACGGCTTGCCAGCGGAGCGTTAGAACTCATATCGATCAATACCGTACCTgg > 2:178901/1‑140 (MQ=255) aGCGTACCGTCGATGGCTTTCGGTTCACCGCCGCTCACCGGAGCATCCAGCATATCAATGCCTTTCGCTTTCAGCGCTTCGCTGATTTCACGGCTTGCCAGCCGCGCGATAGAACTCTTATCGAGCAATACCGTACCTgg > 1:254999/1‑140 (MQ=255) cGTACCGTCGATGGCTTTCGGTTCACCGCCGCTCACCGGAGCATCCAGCATATCAATGCCTTTCGCTTTCAGCGCTTCGCTGATTTCACGGCTTGCCAGCGGCGCGATAGAACTCATATCGATCAATACCGTACCTGGCt > 2:495609/1‑140 (MQ=255) cGTACCGTCGATGGCTTTCGGTTCACCGCCGCTCACCGGAGCATCCAGCATATCAATGCCTTTCGCTTTCAGCGCTTCGCTGATTTCACGGCTTGCCAGCGGCGCGATAGAACTCATATCGATCAATACCGTACCTGGCt > 2:340610/1‑140 (MQ=255) tACCGTCGATGGCTTTCGGTTCACCGCCGCTCACCGGAGCATCCAGCATATCAATGCCTTTCGCTTTCAGCGCTTCGCTGATTTCACGGCTTGCCAGCGGCGCGATAGAACTCATATCGATCAATACCGTACCTGGCTTc > 2:443426/1‑140 (MQ=255) tACCGTCGATGGCTTTCGGTTCACCGCCGCTCACCGGAGCATCCAGCATATCAATGCCTTTCGCTTTCAGCGCTTCGCTGATTTCACGGCTTGCCAGCGGCGCGATAGAACTCATATCGATCAATACCGTACCTGGCTTc > 2:20517/1‑140 (MQ=255) tACCGTCGATGGCTTTCGGTTCACCGCCGCTCACCGGAGCATCCAGCATATCAATGCCTTTCGCTTGCAGCGCTTCTCTGATTTCACGTCTTGCCAGCGGCGCGATAGAACTCATATCGATCAATGCCTTAGCGGGCTTc > 2:410830/1‑140 (MQ=255) ccGTCGATGGCTTTCGGTTCACCGCCGCTCACCGGAGCATCCAGCATATCAATGCCTTTCGCTTTCAGCGCTTCGCTGATTTCACGGCTTGCCAGCGGCGCGATAGAACTCATATCGATCAATACCGTACCTGGCTTcgc < 1:443426/140‑1 (MQ=255) cGTCGATGGCTTTCGGTTCACCGCCGCTCACCGGAGCATCCAGCATATCAATGCCTTTCGCTTTCAGCGCTTCGCTGATTTCACGGCTTGCCAGCGGCGCGATAGAACTCATATCGATCAATACCGTACCTGGCTTcgcg > 2:579581/1‑140 (MQ=255) gTCGATGGCTTTCGGTTCACCGCCGCTCACCGGAGCATCCAGCATATCAATGCCTTTCGCTTTCAGCGCTTCGCTGATTTCACGGCTTGCCAGCGGCGCGATAGAACTCATATCGATCAATACCGTACCTGGCTTCgcgc > 1:563827/1‑140 (MQ=255) cGGTTCACCGCCGCTCATCGGATCATCCAGCTTATGAATCCCTTTCGCTTTCAGCGCTTCGCTGATTTCACGGCTTGCCAGCGGCGCGATAGAACTCATATCGATCAATACCGTACCTGGCTTCGCGCCTTCAATAATGc < 2:254999/140‑1 (MQ=255) cGGTTCACCGCCGCTCACCGGAGCATCCAGCATATCAATGCCTTTCGCTTTCAGCGCTTCGCTGATTTCACGGCTTGCCAGCGGCGCGATAGAACTCATATCGATCAATACCGTACCTGGCTTCGCGCCTTCAATAATGc < 1:82640/140‑1 (MQ=255) aCCGGAGCATCCAGCGTATCAATGCATTTCGCTTTCACCGCTTCGCTGATTTCACGGCTTGCCAGCGGCGCGATAGAACTCATATCGATCAATACCGTACCTGGCTTCGCGCCTTCAATAATGCCATTCTCACCCAgcgc < 1:233257/140‑1 (MQ=255) atCAATGCCTTTCGCTTTCAGCGCTTCGCTGATTTCACGGCTTGCCAGCGGCGCGATAGAACTCATATCGATCAATACCGTACCTGGCTTCGCGCCTTCAATAATGCCATTCTCACCCAGCGCCACCTCTTTCACATGAg > 2:329386/1‑140 (MQ=255) atCAATGCCTTTCGCCTTCAGCACTACGCAGATTTCAAGGCTTGCCAGCGGCGCGATAGAACTCATATCGATCAATACCGTACCTGGCTTCGCGCCTTCAATAATGCCATTCTCACCCAGCGCCACCTCTTTCACATGAg < 1:203955/140‑1 (MQ=255) cAATGCCTTTCGCTTTCAGCGCTTCGCTGATTTCACGGCTTGCCAGCGGCGCGATAGAACTCATATCGATCAATACCGTACCTGGCTTCGCGCCTTCAATAATGCCATTCTCACCCAGCGCCACCTCTTTCACATGAggg > 2:435521/1‑140 (MQ=255) ttCGCTTTCAGCGCTTCGCTGATTTCACGGCTTGCCAGCGGCGCGATAGAACTCATATCGATCAATACCGTACCTGGCTTCGCGCCTTCAATAATGCCATACTCACCCCGCGCCACCACTTTGACATGAGGGGAGTTTgg > 2:189210/1‑140 (MQ=255) ttCGCTTTCAGCGCTTCGCTGATTTCACGACTTGCCACCGGCGCGATAGAACTCATATCGATCAATACCGTACCTGGCTTCGCGCCTTCAATAATGCCATTCTCACCCAGCGCCACCTCTTTCACATGAGGGGAGTTTgg < 1:178901/140‑1 (MQ=255) tCGCTTTCAGCGCTTCGCTGATTTCACGGCTTGCCAGCGGCGCGATAGAACTCATATCGATCAATACCGTACCTGGCTTCGCGCCTTCAATAATGCCATTCTCACCCAGCGCCACCTCTTTCACATGAGGGGAGTTTGgc > 1:536008/1‑140 (MQ=255) tCGCTTTCAGCGCTTCGCTGATTTCACGGCTTGCCAGCGGCGCGATAGAACTCATATCGATCAATACCGTACCTGGCTTCGCGCCTTCAATAATGCCATTCTCACCCAGCACCACCTCTTTCACATGAGGGGAGTTTGgc > 1:289731/1‑140 (MQ=255) tttCAGCGCTTCGCTGATTTCACGGCTTGCCAGCGGCGCGATAGAACTCTTATCGATCAATACCGTACCTGGCTTCGCGCCTTCAATAATGCCATTCTCACCCAGCGCCACCTCTTTCACATGAGGGGAGTTTGgcagca < 1:644750/140‑1 (MQ=255) tttCAGCGCTTCGCTGATTTCACGGCTTGCCAGCGGCGCGATAGAACTCATATCGATCAATACCGTACCTGGCTTCGCGCCTTCAATAATGCCATTCTCACCCAGCGCCACCTCTTTCACATGAGGGGAGTTTGgcagca < 2:167163/140‑1 (MQ=255) tttCAGCGATTCGCTGATTTCATGGCTTGCCTGGGCCGCCATAGAACTCATATCGATCAATACCGTACCTGGCTTCGCGCCTTCAATAATGCCATTCTCACCCAGCGCCACCTCTTTCACATGAGGGGAGTTTGgcagca < 2:305834/140‑1 (MQ=255) tCAGCGCTTCGCTGATTTCACGGCTTGCCAGCGGCGCGATAGAACTCATATCGATCAATACCGTACCTGGCTTCGCGCCTCCAATAATGCCATTCTCACCCAGCGCCACCTCTTTCACATGAGGGGAGTTTGGCAGCATg > 1:530435/1‑140 (MQ=255) tGATTTCACGGCTTGCCAGCGGCGCGATAGAACTCATATCGATCAATACCGTACCTGGCTTCGCGCCTTCAATAATGCCATTCTCACCCAGCGCCACCTCTTTCACATGAGGGGAGTTTGGCAGCATGGTTATGATGACg < 1:579581/140‑1 (MQ=255) tGATTTCACGGCTTGCCAGCGGCGCGATAGAACTCATATCGATCAATACCGTACCTGGCTTCGCGCCTTCAATAATGCCATTCTCACCCAGCGCCACCTCTTTCACATGAGGGGAGTTTGGCAGCATGGTTATGATGACg < 1:435521/140‑1 (MQ=255) tCACGGCTTGCCAGCGGCGCGATAGAACTCATATCGATCAATACCGTACCTGGCTTCGCGCCTTCAATAATGCCATTCTCACCCAGCGCCACCTCTTTCACATGAGGGGAGTTTGGCAGCATGGTTATGATGACGTCGCa < 2:536008/140‑1 (MQ=255) tCACGGCTTGCCAGCGGCGCGATAGAACTCAAATCAATCAATACCGTACCTGGCTTCGCGCCTTCAATAATGCCATTCTCACCCAGCGCCACCTCTTTCACATGAGGGGAGTTTGGCAGCATGGTTATGATGACGTCGCa < 2:229378/140‑1 (MQ=255) | AAATAGCCTTGTCGCCGCCCACCATCACTGACAGCGTACCGTCGATGGCTTTCGGTTCACCGCCGCTCACCGGAGCATCCAGCATATCAATGCCTTTCGCTTTCAGCGCTTCGCTGATTTCACGGCTTGCCAGCGGTGCGATAGAACTCATATCGATCAATACCGTACCTGGCTTCGCGCCTTCAATAATGCCATTCTCACCCAGCGCCACCTCTTTCACATGAGGGGAGTTTGGCAGCATGGTTATGATGACGTCGCA > NC_000913/3272325‑3272583 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |