Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,310,823	T→C	T116A (ACC→GCC)	pnp ←	polynucleotide phosphorylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,310,823	0	T	C	100.0%	92.2 / NA	27	T116A (ACC→GCC)	pnp	polynucleotide phosphorylase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (16/11);  total (16/11)

ACTGGTCATTGATGTAACCTACGCGGGCAGCACCAATCGGGCCATTGAACGGAATACCAGACAGAGACAGCGCTGCGGAAGCACCAATCATCGCGACGATATCCGGGTTAACTTGCGGGTTAACAGAAACCACGGTGGCGATAACCTGAACTTCGTTGACGAAGCCTTCCGGGAACAGCGGGCGAATCGGGCGGTCAATCAGACGCGCGATCAGGGTTTCGCCTTCGCTTGGGCGGCCTTCACGACGGAAGAAGCTACCCGGGATACG  >  NC_000913/3310688‑3310955                                                                                                                                        |                                                                                                                                     acTGGTCATTGATGTAACCTACGCGGGCAGCACCAATCGGGCCATTGAACGGAATACCAGACAGAGACAGCGCTGCGGAAGCACCAATCATCGCGACGATATCCGGGTTAACTTGCGGGTTAACAGAAACCACGgcggcg                                                                                                                                  <  2:160089/140‑1 (MQ=255)   tGGTCATTGATGTAATCTACGCAGGTAGCACCAATCGCGCCATTTAACGGAATACCAGACAGAGACAGCGCTGCGGAAGCACCAATCATCGCGACGATATCCGGGTTAACTTGCGGGTTAACAGAAACCACGGCGGCGAt                                                                                                                                <  1:183186/140‑1 (MQ=255)   tGGTCATTGATGTAACCTACGCGGGCAGCACCAATCGGGCCATTGAACGGAATACCAGACAGAGACAGCGCTGCGGAAGCACCAATCATCGCGACGATATCCGGGTTAACTTGCGGGTTAACAGAAACCACGGCGGCGAt                                                                                                                                >  2:664127/1‑140 (MQ=255)    ggTCATTGATGTAACCTACGCGGGCAGCACCAATCGGGCCATTGAACGGAATACCAGACAGAGACAGCGCTGCGGAAGCACCAATCATCGCGACGATATCCGGGTTAACTTGCGGGTTAACAGAAACCACGGCGGCGATa                                                                                                                               >  1:360897/1‑140 (MQ=255)    ggTCATTGATGTAACCTACGCGGGCAGCACCAATCGGGCCATTGAACGGAATACCAGACAGAGACAGCGCTGCGGAAGCACCAATCATCGCGACGATATCCGGGTTAACTTGCGGGTTAACAGAAACCACGGCGGCGATa                                                                                                                               >  2:19995/1‑140 (MQ=255)    ggTCATTGATGTAACCTACGCGGGCAGCACCAATCGGGCCATTGAACGGAATACCAGACAGAGACAGCGCTGCGGAAGCACCAATCATCGCGACGATATCCGGGTTAACTTGCGGGTTAACAGAAACCACGGCGGCGATa                                                                                                                               >  1:112635/1‑140 (MQ=255)    ggTCATTGATGTAACCTACGCGGGCAGCACCAATCGGGCCATTGAACGGAATACCAGACAGAGACAGCGCTGCGGAAGCACCAATCATCGCGACGATATCCGGGTTAACTTGCGGGTTAACAGAAACCACGGCGGCGATa                                                                                                                               >  1:53870/1‑140 (MQ=255)      tCATTGATGTAACCTACGCGGGCAGCACCAATCGGGCCATTGAACGGAATACCAGACAGAGACAGCGCTGCGGAAGCACCAATCATCGCGACGATATCCGGGTTAACTTGCGGGTTAACAGAAACCACGGCGGCGATAAc                                                                                                                             >  2:484334/1‑140 (MQ=255)        aTTGATGTAACCTACGCGGGCAGCACCAATCGGGCCATTGAACGGAATACCAGACAGAGACAGCGCTGCGGAAGCACCAATCATCGCGACGATATCCGGGTTAACTTGCGGGTTAACAGAAACCACGGCGGCGATAACCt                                                                                                                           >  1:631299/1‑140 (MQ=255)                   cTACGCGGGCAGCACCAATCGGGCCATTGAACGGAATACCAGACAGAGACAGCGCTGCGGAAGCACCAATCATCGCGACGATATCCGGGTTAACTTGCGGGTTAACAGAAACCACGGCGGCGATAACCTGAACTTCGTTg                                                                                                                <  1:664127/140‑1 (MQ=255)                      cgcgGGCAGCACCAATCGGGCCATTGAACGGAATACCAGACAGAGACAGCGCTGCGGAAGCACCAATCATCGCGACGATATCCGGGTTAACTTGCGGGTTAACAGAAACCACGGCGGCGATAACCTGAACTTCGTTGACg                                                                                                             >  2:513789/1‑140 (MQ=255)                       gcgGGCAGCACCAATCGGGCCATTGAACGGAATACCAGACAGAGACAGCGCTGCGGAAGCACCAATCATCGCGACGATATCCGGGTTAACTTGCGGGTTAACAGAAACCACGGCGGCGATAACCTGAACTTCGTTGACGa                                                                                                            >  1:399492/1‑140 (MQ=255)                          ggCAGCACTAATCGGGCAATTGCACGGAATAGCAGTCAGAGACAGCGCTGCGGAAGCACCAATCATCGCGACGATATCCGGGTTAACTTGCGGGTTAACAGAAACCACGGCGGCGATAACCTGAACTTCGTTGACGAAGc                                                                                                         <  2:310399/140‑1 (MQ=255)                                                   ggAATACCAGACAGAGACAGCGCTGCGGAAGCACCAATCATCGCGACGATATCCGGGTTAACTTGCGGGTTAACAGAAACCACGGCGGCGATAACCTGAACTTCGTTGACGAAGCCTTCCGGGAACAGCGGGCGAATCgg                                                                                <  1:3266/140‑1 (MQ=255)                                                               agagacagCGCTGCGGAAGCACCAATCATCGCGACGATATCCGGGTTAACTTGCGGGTTAACAGAAACCACGGCGGCGATAACCTGAACTTCGTTGACGAAGCCTTCCGGGAACAGCGGGCGAATCGGGCGGTCAATCAg                                                                    >  2:167732/1‑140 (MQ=255)                                                               agagacagCGCTGCGGAAGCACCAATCATCGCGACGATATCCGGGTTAACTTGCGGGTTAACAGAAACCACGGCGGCGATAACCTGAACTTCGTTGACGAAGCCTTCCGGGAACAGCGGGCGAATCGGGCGGTCAATCAg                                                                    >  2:557906/1‑140 (MQ=255)                                                                 agacagCGCTGCGGAAGCACCAATCATCGCGACGATATCCGGGTTAACTTGCGGGTTAACAGAAACCACGGCGGCGATAACCTGAACTTCGTTGACGAAGCCTTCCGGGAACAGCGGGCGAATCGGGCGGTCAATCAGAc                                                                  >  2:493541/1‑140 (MQ=255)                                                                  gacagCGCTGCGGAAGCACCAATCATCGCGACGATATCCGGGTTAACTTGCGGGTTAACAGAAACCACGGCGGCGATAACCTGAACTTCGTTGACGAAGCCTTCCGGGAACAGCGGGCGAATCGGGCGGTCAATCAGAcg                                                                 <  2:631299/140‑1 (MQ=255)                                                                       cgcTGCGGAAGCACCAATCATCGCGACGATATCCGGGTTAACTTGCGGGTTAACAGAAACCACGGCGGCGATAACCTGAACTTCGTTGACGAAGCCTTCCGGGAACAGCGGGCGAATCGGGCGGTCAATCAGACGCGCGa                                                            >  1:535493/1‑140 (MQ=255)                                                                         cTGCGGAAGCACCAATCATCGCGACGATATCCGGGTTAACTTGCGGGTTAACAGAAACCACGGCGGCGATAACCTGAACTTCGTTGACGAAGCCTTCCGGGAACAGCGGGCGAATCGGGCGGTCAATCAGACGCGCGATc                                                          <  2:360897/140‑1 (MQ=255)                                                                            cGGAAGCACCAATCATCGCGACGATATCCGGGTTAACTTGCGGGTTAACAGAAACCACGGCGGCGATAACCTGAACTTCGTTGACGAAGCCTTCCGGGAACAGCGGGCGAATCGGGCGGTCAATCAGACGCGCGATCAgg                                                       >  2:593603/1‑140 (MQ=255)                                                                                            cgcgACGATATCCGGGTTAACTTGCGGGTTAACAGAAACCACGGCGGCGATAACCTGAACTTCGTTGACGAAGCCTTCCGGGAACAGCGGGCGAATCGGGCGGTCAATCAGACGCGCGATCAGGGTTTCGCCTTCGCTTg                                       >  1:414904/1‑140 (MQ=255)                                                                                                          ggTTAACTTGCGGGTTAACAGAAACCACGGCGGCGATAACCTGAACTTCGTTGACGAAGCCTTCCGGGAACAGCGGGCGAATCGGGCGGTCAATCAGACGCGCGATCAGGGTTTCGCCTTCGCTTGGGCGGCCTTCacga                         <  1:513789/140‑1 (MQ=255)                                                                                                                        ttAACAGAAACCACGGCGGCGATAACCTGAACTTCGTTGACGAAGCCTTCCGGGAACAGCGGGCGAATCGGGCGGTCAATCAGACGCGCGATCAGGGTTTCGCCTTCGCTTGGGCGGCCTTCACGACGGAAGAAGCTAcc           <  1:85485/140‑1 (MQ=255)                                                                                                                        ttAACAGAAACCACGGCGGCGATAACCTGAACTTCGTTGACGAAGCCTTCCGGGAACAGCGGGCGAATCGGGCGGTCAATCAGACGCGCGATCAGGGTTTCGCCTTCGCTTGGGCGGCCTTCACGACGGAAGAAGCTAcc           <  2:399492/140‑1 (MQ=255)                                                                                                                        ttAACAGAAACCACGGCGGCGATAACCTGAACTTCGTTGACGAAGCCTTCCGGGAACAGCGGGCGAATCGGGCGGTCAATCAGACGCGCGATCAGGGTTTCGCCTTCGCTTGGGCGGCCTTCACGACGGAAGAAGCTAcc           <  2:414904/140‑1 (MQ=255)                                                                                                                         tAACAGAAACCACGGCGGCGATAACCTGAACTTCGTTGACGAAGCCTTCCGGGAACAGCGGGCGAATCGGGCGGTCAATCAGACGCGCGATCAGGGTTTCGCCTTCGCTTGGGCGGCCTTCACGACGGAAGAAGCTAccc          <  1:392781/140‑1 (MQ=255)                                                                                                                                 aCCACGGCGGCGATAACCTGAACTTCGTTGACGAAGCCTTCCGGGAACAGCGGGCGAATCGGGCGGTCAATCAGACGCGCGATCAGGGTTTCGCCTTCGCTTGGGCGGCCTTCACGACGGAAGAAGCTACCCGGGATACg  >  1:81971/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                     ACTGGTCATTGATGTAACCTACGCGGGCAGCACCAATCGGGCCATTGAACGGAATACCAGACAGAGACAGCGCTGCGGAAGCACCAATCATCGCGACGATATCCGGGTTAACTTGCGGGTTAACAGAAACCACGGTGGCGATAACCTGAACTTCGTTGACGAAGCCTTCCGGGAACAGCGGGCGAATCGGGCGGTCAATCAGACGCGCGATCAGGGTTTCGCCTTCGCTTGGGCGGCCTTCACGACGGAAGAAGCTACCCGGGATACG  >  NC_000913/3310688‑3310955

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑