| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,337,189 | T→C | D23G (GAC→GGC) | mlaB ← | intermembrane phospholipid transport system protein MlaB |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,337,189 | 0 | T | C | 98.0% | 172.1 / NA | 50 | D23G (GAC→GGC) | mlaB | intermembrane phospholipid transport system protein MlaB |
| Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (26/23); total (26/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.80e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CTGCTTTTTCGCCAGATCGATAAGATGGAGCAGCAGTGCCAGTCCCCCCGTATCCACGCGGGAGACACGGCTAAGATCGATGCAGGTAATCCCCTTCACCGCTTCCTCACGCATTTCCCAAAGCGGTAGCAAAACGTCCTGATCCAGCTCTCCGGATAACGCCAGCGTGTCACCCGTCTGCATCCAGCTCAGTGACTCGCTCATTATTTTTTCTCTTCCAGAGTGATTTTCTGTTGAGAAATCGATTTCAGTTGCGCAGTCAGGCCGTCGAT > NC_000913/3337053‑3337324 | cTGCTTTTTCGCCAGATCGATAAGATGGAGCAGCAGTGCCAGTCCCCCCGTATCCACGCGGGAGACACGGCTAAGATCGATGCAGGTAATCCCCTTCACCGCTTCCTCACGCATTTCCCAAAGCGGTAGCAAAACGCCCt > 1:641374/1‑140 (MQ=255)cTGCTTTTTCGCCAGATCGATAAGATGGAGCAGCAGTGCCAGTCCCCCCGTATCCACGCGGGAGACACGGCTAAGATCGATGCAGGTAATCCCCTTCACCGCTTCCTCACGCATTTCCCAAAGCGGTAGCAAAACGCCCt > 2:583389/1‑140 (MQ=255) tGCTTTTTCGCCAGATCGATAAGATGGAGCAGCAGTGCCAGTCCCCCCGTATCCACGCGGGAGACACGGCTAAGATCGATGCAGGTAATCCCCTTCACCGCTTCCTCACGCATTTCCCAAAGCGGTAGCAAAACGCCCTg < 2:82136/140‑1 (MQ=255) aGATCGATAAGATGGAGCAGCAGTGCCAGTCCCCCCGTATCCACGCGGGAGACACGGCTAAGATCGATGCAGGTAATCCCCTTCACCGCTTCCTCACGCATTTCCCAAAGCGGTAGCAAAACGCCCTGATCCAGCTCTcc > 2:551702/1‑140 (MQ=255) gATGGAGCAGCAGTGCCAGTCCCCCAGTATCCACGCGCGAGACACGGCTAAGATCGATGCAGGTAATCCCCTTCACCGCTTCCTCACGCATTTCCCAAAGCGGTAGCAAAACGCCCTGATCCAGCTCTCCGGATAACGcc < 1:220063/140‑1 (MQ=255) tGGAGCAGCAGTGCCAGTCCCCCCGTATCCACGCGGGAGACACGGCTAAGATCGATGCAGGTAATCCCCTTCACCGCTTCCTCACGCATTTCCCAAAGCGGTAGCAAAACGCCCTGATCCAGCTCTCCGGATAACGCCAg > 2:512585/1‑140 (MQ=255) gAGCAGCAGTGCCAGTCCCCCCGTATCCACGCGGGAGACACGGCTAAGATCGATGCAGGTAATCCCCTTCACCGCTTCCTCACGCATTTCCCAAAGCGGTAGCAAAACGCCCTGATCCAGCTCTCCGGATAACGCCAGCg > 1:335155/1‑140 (MQ=255) agcagcAGTGCCAGTCCCCCCGTATCCACGCGGGAGACACGGCTAAGATCGATGCAGGTAATCCCCTTCACCGCTTCCTCACGCATTTCCCAAAGCGGTAGCAAAACGCCCTGATCCAGCTCTCCGGATAACGCCATCgt > 1:17844/1‑140 (MQ=255) gcagcaGTGCCAGTCCCCCCGTATCCACGCGGGAGACACGGCTAAGATCGATGCAGGTAATCCCCTTCACCGCTTCCTCACGCATTTCCCAAAGCGGTAGCAAAACGCCCTGATCCAGCTCTCCGGATAACGCCAGCgtg > 1:516645/1‑140 (MQ=255) ccAGTCCCCCCGTATCCACGCGGGAGACACGGCTAAGATCGATGCAGGTAATCCCCTTCACCGCTTCCTCACGCATTTCCCAAAGCGGTAGCAAAACGCCCTGATCCAGCTCTCCGGATAACGCCAGCGTGTCACCCGt > 1:329744/1‑139 (MQ=255) ccAGTCCCCCCGTATCCACGCGGGAGACACGGCTAAGATCGATGCAGGTAATCCCCTTCACCGCTTCCTCACGCATTTCCCAAAGCGGTAGCAAAACGCCCTGATCCAGCTCTCCGGATAACGCCAGCGTGTCACCCGt < 2:329744/139‑1 (MQ=255) ccAGTCCCCCCGTATCCACGCGAGAGACACGGCTAAGATCGATGCAGGTAATCCCCTTCACCGCTTCCTCACGCATTTCCCAAAGCGGTAGCAAAACGCCCTGATCCAGCACTCAGGATAACGCGAGCGTGTCACCCGTc > 1:280896/1‑140 (MQ=255) cAGTCCCCCCGTATCCACGCGGGAGACACGGCTAAGATCGATGCAGGTAATCCCCTTCACCGCTTCCTCACGCATTTCCCAAAGCGGTAGCAAAACGCCCTGATCCAGCTCTCCGGATAACGCCAGCGTGTCACCCGTCt < 2:441500/140‑1 (MQ=255) ccccccGTATCCACGCGGGAGACACGGCTAAGATCGATGCAGGTAATCCCCTTCACCGCTTCCTCACGCATTTCCCAAAGCGGTAGCAAAACGCCCTGATCCAGCTCTCCGGATAACGCCAGCGTGTCACCCGTCTGCAt > 1:401908/1‑140 (MQ=255) ccccGTATCCACGCGGGAGACACGGCTAAGATCGATGCAGGTAATCCCCTTCACCGCTTCCTCACGCATTTCCCAAAGCGGTAGCAAAACGCCCTGATCCAGCTCTCCGGATAACGCCAGCGTGTCACCCGTCTGCATcc < 2:661093/140‑1 (MQ=255) ccGTATCCACGCGGGAGACACGGCTAAGATCCATGCAGGCAATCCCCTCCACCGCTTCCTCACGCATTTCCCAAAGCGGTAGCAAAACGCCCTGATCCAGCTCTCCGGATAACGCCAGCGTGTCACCCGTCTGCATCCAg < 2:237464/140‑1 (MQ=255) aTCCACGCGGGAGACACGGCTAAGATCGATGCAGGTAATCCCCTTCACCGCTTCCTCACGCATTTCCCAAAGCGGTAGCAAAACGCCCTGATCCAGCTCTCCGGATAACGCCAGCGTGTCACCCGTCTGCATCCAGCTCa < 1:658722/140‑1 (MQ=255) aCGCGGGAGACACGGCTAAGATCGATGCAGGTAATCCCCTTCACCGCTTCCTCACGCATTTCCCAAAGCGGTAGCAAAACGCCCTGATCCAGCTCTCCGGATAACGCCAGCGTGTCACCCGTCTGCATCCAGCTCAGTGa < 2:383363/140‑1 (MQ=255) ggcagaCACGGCTAAGATCGATGCAGGTAATCCCCTTCACCGCTTCCTCACGCATTTCCCAAAGCGGTAGCAAAACGCCCTGATCCAGCTCTCCGGATAACGCCAGCGTGTCACCCGTCTGCATCCAGCTCAGTGACTCg < 2:401908/137‑1 (MQ=255) ggAGACACGGCTAAGATCGATGCAGGTAATCCCCTTCACCGCTTCCTCACGCATTTCCCAAAGCGGTAGCAAAACGCCCTGATCCAGCTCTCCGGATAACGCCAGCGTGTCACCCGTCTGCATCCAGCTCAGTGACTCGc > 2:472374/1‑140 (MQ=255) gaCACGGCTAAGATCGATGCAGGTAATCCCCTTCACCGCTTCCTCACGCATTTCCCAAAGCGGTAGCAAAACGCCCTGATCCAGCTCTCCGGATAACGCCAGCGTGTCACCCGTCTGCATCCAGCTCAGTGACTCGCTCa < 1:583389/140‑1 (MQ=255) ggCTAAGATCGATGCAGGTAATCCCCTTCACCGCTTCCTCACGCATTTCCCAAAGCGGTAGCAAAACGCCCTGATCCAGCTCTCCGGATAACGCCAGCGTGTCACCCGTCTGCATCCAGCTCAGTGACTCGCTCattatt < 2:95865/140‑1 (MQ=255) gATGCAGGTAATCCCCTTCACCGCTTCCTCACGCATTTCCCAAAGCGGTAGCAAAACGCCCTGATCCAGCTCTCCGGATAACGCCAGCGTGTCACCCGTCTGCATCCAGCTCAGTGACTCGCTCATTATTTTTTCTCTTc > 1:650519/1‑140 (MQ=255) gATGCAGGTAATCCCCTTCACCGCTTCCTCACGCATTTCCCAAAGCGGTAGCAAAACGCCCTGATCCAGCTCTCCGGATAACGCCAGCGTGTCACCCGTCTGCATCCAGCTCAGTGACTCGCTCATTATTTTTTCTCTTc < 2:397743/140‑1 (MQ=255) gATGCAGGTAATCCCCTTCACCGCTTCCTCACGCATTTCCCAAAGCGGTAGCAAAACGCCCTGATCCAGCTCTCCGGATAACGCCAGCGTGTCACCCGTCTGCATCCAGCTCAGTGACTCGCTCATTATTTTTTCTCTTc < 1:472374/140‑1 (MQ=255) tGCAGGTAATCCCCTTCACCGCTTCCTCACGCATTTCCCAAAGCGGTAGCAAAACGCCCTGATCCAGCTCTCCGGATAACGCCAGCGTGTCACCCGTCTGCATCCAGCTCAGTGACTCGCTCATTATTTTTTCTCTTCCa < 1:516694/140‑1 (MQ=255) tGCAGGTAATCCCCTTCACCGCTTCCTCACGCATTTCCCAAAGCGGTAGCAAAACGCCCTGATCCAGCTCTCCGGATAACGCCAGCGTGTCACCCGTCTGCATCCAGCTCAGTGACTCGCTCATTATTTTTTCTCTTCCa > 1:447196/1‑140 (MQ=255) ttCACCGCTTCCTCACGCATTTCCCAAAGCGGTAGCAAAACGCCCTGATCCAGCTCTCCGGATAACGCCAGCGTGTCACCCGTCTGCATCCAGCTCAGTGACCCGCTCATTATTTTTACTCTTCTAGAGTGATTTTCTGt > 1:303011/1‑140 (MQ=255) tCACCGCTTCCTCACGCATTTCCCAAAGCGGTAGCAAAACGCCCTGATCCAGCTCTCCGGATAACGCCAGCGTGTCACCCGTCTGCATCCAGCTCAGTGACTGGCTCATTATTTTTTTTCTTCCGGAGTGATTTTCTGtt > 1:288007/1‑140 (MQ=255) tCACCGCTTCCTCACGCATTTCCCAAAGCGGTAGCAAAACGCCCTGATCCAGCTCTCCGGATAACGCCAGCGTGTCACCCGTCTGCATCCAGCTCAGTGACTCGCTCATTATTTTTTCTCTTCCa < 1:375702/125‑1 (MQ=255) tCACCGCTTCCTCACGCATTTCCCAAAGCGGTAGCAAAACGCCCTGATCCAGCTCTCCGGATAACGCCAGCGTGTCACCCGTCTGCATCCAGCTCAGTGACTCGCTCATTATTTTTTCTCTTCCa > 2:375702/1‑125 (MQ=255) tCACCGCTTCCTCACGCATTTCCCAAAGCGGTAGCAAAACGCCCTGATCCAGCTCTCCGGATAACGCCAGCGTGTCACCCGTCTGCATCCAGCTCAGTGACTCGCTCATTATTTTTTCTCTTCCAGAGTGATTTTCTGtt > 1:134101/1‑140 (MQ=255) cTTCCTCACGCATTTCCCAAAGCGGTAGCAAAACGCCCTGATCCAGCTCTCCGGATAACGCCAGCGTGTCACCCGTCTGCATCCAGCTCAGTGACTCGCTCATTATTTTTTCTCTTCCAGAGTGATTTTCTGTTGAGaaa > 2:443584/1‑140 (MQ=255) cTTCCTCACGCATTTCCCAAAGCGGTAGCAAAACGCCCTGATCCAGCTCTCCGGATAACGCCAGCGTGTCACCCGTCTGCATCCAGCTCAGTGACTCGCTCATTATTTTTTCTCTTCCAGAGTGATTTTCTGTTGAGaaa > 1:514575/1‑140 (MQ=255) ccTCACGCATTTCCCAAAGCGGTAGCAAAACGCCCTGATCCAGCTCTCCGGATAACGCCAGCGTGTCACCCGTCTGCATCCAGCTCAGTGACTCGCTCATTAATTTTTCTGTTCCAGAGTGATTATCTGTTGAGAAATCg > 1:181131/1‑140 (MQ=255) cACGCATTTCCCAAAGCGGTAGCAAAACGCCCTGATCCAGCTCTCCGGATAACGCCAGCGTGTCACCCGTCTGCATCCAGCTCAGTGACTCGCTCATTATTTTTTCTCCTCCAGAGTGATTTGCTGTTGAGAAATCGAtt > 2:264671/1‑140 (MQ=255) cGCATTTCCCAAAGCGGTAGCAAAACGCCCTGATCCAGCTCTCCGGATAACGCCAGCGTGTCACCCGTCTGCATCCAGCTCAGTGACTCGCTCATTATTTTTTCTCTTCCAGAGTGATTTTCTGTTGAGAAATCGATTTc > 1:77057/1‑140 (MQ=255) tttCCCAAAGCGGTAGCAAAACGCCCTGATCCAGCTCTCCGGATAACGCCAGCGTGTCACCCGTCTGCATCCAGCTCAGTGACTCGCTCATTATTTTTTCTCTTCCAGAGTGATTTTCTGTTGAGAAATCGATTTCAGtt < 1:443584/140‑1 (MQ=255) ttCCCAAAGCGGTAGCACAACGTCCTGATCCCGCTCTCCTGATAACGCCAGCGTGTCACCCGTCTGCATCCAGCTCAGTGACTCGCTCATTATTTTTTCTCTTCCAGAGTGATTTTCTGTTGAGAAATCGATTTCAGTTg < 2:181131/140‑1 (MQ=255) ttCCCAAAGCGGTAGCAAAACGCCCTGATCCAGCTCTCCGGATAACGCCAGCGTGTCACCCGTCTGCATCCAGCTCAGTGACTCGCTCATTATTTTTTCTCTTCCAGAGTGATTTTCTGTTGAGAAATCGATTTCAGTTg < 2:335155/140‑1 (MQ=255) ttCCCAAAGCGGTAGCAAAACGCCCTGATCCAGCTCTCCGGATAACGCCAGCGTGTCACCCGTCTGCATCCAGCTCAGTGACTCGCTCATTATTTTTTCTCTTCCAGAGTGATTTTCTGTTGAGAAATCGATTTCAGTTg > 1:538849/1‑140 (MQ=255) ggTAGCAAAACGCCCTGATCCAGCTCTCCGGATAACGCCAGCGTGTCACCCGTCTGCATCCAGCTCAGTGACTCGCTCATTATTTTTTCTCTTCCAGAGTGATTTTCTGTTGAGAAATCGATTTCAGTTGCGCAGTCAgg > 1:475314/1‑140 (MQ=255) gTAGCAAAACGCCCTGATCCAGCTCTCCGGATAACGCCAGCGTGTCACCCGTCTGCATCCAGCTCAGTGACTCGCTCATTATTTTTTCTCTTCCAGAGTGATTTTCTGTTGAGAAATCGATTTCAGTTGCGCAGTCAGGc < 2:516645/140‑1 (MQ=255) tAGCAAAACGCCCTGATCCAGCTCTCCGGATAACGCCAGCGTGTCACCCGTCTGCATCCAGCTCAGTGACTCGCTCATTATTTTTTCTCTTCCAGAGTGATTTTCTGTTGCGAACTCGATTTCAATTGCGCAGTCAGGcc > 1:260703/1‑140 (MQ=255) aaaCGCCCTGATCCAGCTCTCCTGATAACGCTATCTTGTTACCCGTCTTCATCCAGCTCAGTGACTCGCTCATTATTTTTTCTCTTCCAGAGTGATTTTCTGTTGAGAAATCGATTTCAGTTGCGCAGTCAGGCCGTCGa < 2:288007/140‑1 (MQ=255) aaaCGCCCTGATCCAGCTCTCCTGATAACGCCAGCGTGTCACCCGTCTGCATCCAGCTCAGTGACTCGCTCATTATTTTTTCTCTTCCAGAGTGATTTTCTGTTGAGAAATCGATTTCAGTTGCGCAGTCAGGCCGTCGa < 1:298609/140‑1 (MQ=255) aaaCGCCCTGATCCAGCTCTCCGGATAACGCCAGCGTGTCACCCGTCTGCATCCAGCTCAGTGACTCGCTCATTATTTTTTCTCTTCCAGAGTGATTTTCTGTTGAGAAATCGATTTCAGTTGCGCAGTCAGGCCGTCGa < 2:17844/140‑1 (MQ=255) aaaCGCCCTGATCCAGCTCTCCGGATAACGCCAGCGTGTCACCCGTCTGCATCCAGCTCAGTGACTCGCTCATTATTTTTTCTCTTCCAGAGTGATTTTCTGTTGAGAAATCGATTTCAGTTGCGCAGTCAGGCCGTCGa < 1:512585/140‑1 (MQ=255) aaaCGCCCTGATCCAGCTCTCCGGATAACGCCAGCGTGTCACCCGTCTGCATCCAGCTCAGTGACTCGCTCATTATTTTTTCTCTTCCAGAGTGATTTTCTGTTGAGAAATCGATTTCAGTTGCGCAGTCAGGCCGTCGa < 1:551702/140‑1 (MQ=255) aaCGCCCTGATCCAGCTCTCCGGATAACGCCAGCGTGTCACCCGTCTGCATCCAGCTCAGTGACTCGCTCATTATTTTTTCTCTTCCAGAGTGATTTTCTGTTGAGAAATCGATTTCAGTTGCGCAGTCAGGCCGTCGAt > 1:657100/1‑140 (MQ=255) | CTGCTTTTTCGCCAGATCGATAAGATGGAGCAGCAGTGCCAGTCCCCCCGTATCCACGCGGGAGACACGGCTAAGATCGATGCAGGTAATCCCCTTCACCGCTTCCTCACGCATTTCCCAAAGCGGTAGCAAAACGTCCTGATCCAGCTCTCCGGATAACGCCAGCGTGTCACCCGTCTGCATCCAGCTCAGTGACTCGCTCATTATTTTTTCTCTTCCAGAGTGATTTTCTGTTGAGAAATCGATTTCAGTTGCGCAGTCAGGCCGTCGAT > NC_000913/3337053‑3337324 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |