Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,368,963	G→A	intergenic (+9/+51)	yhcG → / ← yhcH	DUF1016 domain‑containing protein YhcG/DUF386 domain‑containing protein YhcH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,368,963	0	G	A	100.0%	125.4 / NA	40	intergenic (+9/+51)	yhcG/yhcH	DUF1016 domain‑containing protein YhcG/DUF386 domain‑containing protein YhcH
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (18/22);  total (18/22)

TCTGGCAGGTTTGCCTAACACCGTTCTGGCAAGCGAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGCTCGCCGACAACGCATCCT  >  NC_000913/3368828‑3369086                                                                                                                                        |                                                                                                                            tctGGCAGGTTTGCCTAACATCGTTCTGGCAAGCGAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGATTTc                                                                                                                         >  2:667015/1‑140 (MQ=255)     gCAGGTTTGCCTAACACCGTTCTGGCAAGCGAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGATTTCGAAt                                                                                                                     <  2:650754/140‑1 (MQ=255)       aGGTTTGCCTAACACCGTTCTGGCAAGCGAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGATTTCGAATTc                                                                                                                   <  1:355780/140‑1 (MQ=255)       aGGTTTGCCTAACACCGTTCTGGCAAGCGAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGATTTCGAATTc                                                                                                                   <  1:16376/140‑1 (MQ=255)              ccTAACACCGTTCTGGCAAGCGAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTGATTTCAGCGATTTCGAATTCTGCAGAc                                                                                                            <  2:456276/140‑1 (MQ=255)               cTAACACCGTTCTGGCAAGCGAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGATTTCGAATTCTGCAGACa                                                                                                           >  1:625462/1‑140 (MQ=255)                     ccGTTCTGGCAAGCGAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGATTTCGAATTCTGCAGACAGTGTCt                                                                                                     <  2:61060/140‑1 (MQ=255)                                aGCGAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCCGCGATTTCGAATTCTGTAGACGGTGTCTGCAGAATCttt                                                                                          >  2:242715/1‑140 (MQ=255)                                  cGAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGATTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTaa                                                                                        >  2:600837/1‑140 (MQ=255)                                          aGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGATTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACt                                                                                <  1:667015/140‑1 (MQ=255)                                               cAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGATTTGGAATTCTGCAGACAGTGTCTGTAGAATCTTTAAAACGAACTAAGtt                                                                           >  2:558070/1‑140 (MQ=255)                                                  cTACCTGATGAGAAACGACTCGCAGATGAACTCGTCCGAACACAGGCGCTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGATTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTa                                                                        <  2:262543/140‑1 (MQ=255)                                                   tACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGATTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTaa                                                                       <  2:437628/140‑1 (MQ=255)                                                      cTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGATTTCGGATTCTGCAGACTGTGGCTGAAGAATCTTTTAAATGAACTAAGTTTTAAGcc                                                                    >  2:213863/1‑140 (MQ=255)                                                          tgaGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGATTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTaa                                                                       <  1:368192/133‑1 (MQ=255)                                                          tgaGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGATTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTa                                                                >  2:150855/1‑140 (MQ=255)                                                          tgaGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGATTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTa                                                                >  1:415359/1‑140 (MQ=255)                                                          tgaGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGGTAATTTCAGCGATTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTaa                                                                       >  2:368192/1‑133 (MQ=255)                                                             gaAACGACTCGCAGATGAACTCGTTCGAACCCAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGATTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAAt                                                             <  1:414409/140‑1 (MQ=255)                                                             gaAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGATTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAAt                                                             <  1:30623/140‑1 (MQ=255)                                                              aaaCGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGATTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATc                                                            >  1:537390/1‑140 (MQ=255)                                                                   aCTCGCAGATGAACTCGTTCGATCATAGGTGGTGCTATAGGAAGGCTATAGACGCCGTTAATTTCAGCGATTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCt                                                       <  1:271905/140‑1 (MQ=255)                                                                   aCTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGATTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCt                                                       <  1:535768/140‑1 (MQ=255)                                                                       gCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGATTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAAc                                                   >  2:619691/1‑140 (MQ=255)                                                                            tGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGATTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTGTTAAGCTATTAAAGCAGCCTTAACCTTca                                              >  1:178950/1‑140 (MQ=255)                                                                              aaCTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGATTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTcacc                                            >  1:656889/1‑140 (MQ=255)                                                                                  cGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGATTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACaa                                        <  2:26981/140‑1 (MQ=255)                                                                                          cacaGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGATTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTa                                <  1:666743/140‑1 (MQ=255)                                                                                            caGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGATTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAAt                              <  2:415359/140‑1 (MQ=255)                                                                                            caGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGATTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAAt                              <  2:537390/140‑1 (MQ=255)                                                                                            caGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGATTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAAt                              <  2:656889/140‑1 (MQ=255)                                                                                            caGGCGGTGCTAGAGCAAGGCTATACACGCCGTTAATTTCAGCGATTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAAt                              <  1:221407/140‑1 (MQ=255)                                                                                               gCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGATTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAAtctc                           >  2:660428/1‑140 (MQ=255)                                                                                                         agGAAGGCTATAGACGCCGTTAATTTCAGCGATTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAAt                              <  2:605375/127‑1 (MQ=255)                                                                                                         agGAAGGCTATAGACGCCGTTAATTTCAGCGATTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAAt                              >  1:605375/1‑127 (MQ=255)                                                                                                             aGGCTATAGACGCCGTTAATTTCAGCGATTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGCTCGCCGa             >  1:429680/1‑140 (MQ=255)                                                                                                             aGGCTATAGACGCCGTTAATTTCAGCGATTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGCTCGCCGa             >  2:623201/1‑140 (MQ=255)                                                                                                             aGGCTATAGACGCCGTTAATTTCAGCGATTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTGTTAATCTCTCCAGGCTCGCCGa             >  2:265318/1‑140 (MQ=255)                                                                                                                 tataGACGCCGTTAATTCCAGCCATTTCGAAATCTTCAGCCAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGCTCGCCGACAAc         <  2:211790/140‑1 (MQ=255)                                                                                                                   taGACGCCGTTAATTCCAGCGAATTCGAAATCTGCAGCCAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGCTCGCCGACAACGc       <  1:213863/140‑1 (MQ=255)                                                                                                                        gCCGTTAATTTCAGCGATTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGCTCGCCGACAACGCATCCt  >  2:570730/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                            TCTGGCAGGTTTGCCTAACACCGTTCTGGCAAGCGAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGCTCGCCGACAACGCATCCT  >  NC_000913/3368828‑3369086

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑