Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,396,892	T→C	T302A (ACC→GCC)	rng ←	RNase G

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,396,892	0	T	C	100.0%	112.4 / NA	35	T302A (ACC→GCC)	rng	RNase G
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (21/14);  total (21/14)

AATAATGATAATCCCGCCCAGATTACGCAACCGTAACTGGCGAGCGATAGCCTGCGTCGCTTCAATATTGGTATTGAAAATGGTGTCGTCCAGATTGCGATGACCGACAAACGCTCCGGTATTGATGTCCACGGTGGTCATCGCTTCGGTCTGGTCGATAATGAGATAACCACCGGATTTCAGTTCTACTTTGCGTTCCAGCGCTCGCTGGATTTCGTTTTCGACATCAAAGAGATCGAAAATCGGCTGGCGTCCTGTGTAATGCTC  >  NC_000913/3396758‑3397024                                                                                                                                       |                                                                                                                                     aataatGATAATCCCGCCCAGATTACGCAACCGTAACTGGCGAGCGATAGCCTGCGTCGCTTCAATATTGGTATTGAAAATGGTGTCGTCCAGATTGCGATGACCGACAAACGCTCCGGTATTGATGTCCACGGCGGTCa                                                                                                                                 <  1:11402/140‑1 (MQ=255)          aaTCCCGCCCAGATTACGCAACCGTAACTGTGGAGTGATTGCCTGCGTTGCTTCAATATTGGTATTGAAAATGGTGTCGTCCAGATTGCGATGACCGACAAACGCTCC‑GTATTGATGTCCACGGCGGTCATCGCTTCGGt                                                                                                                       <  2:308700/140‑1 (MQ=255)           aTCCCGCCCAGATTACGCAACCGTAACTGGCGAGCGATAGTCTGCGTCGCTTCAATATTGGTATTGAAAATGGTGTCGTCCAGATTGCGATGACCGACAAACGCTCCGGTATTGATGTCCACGGCGGTCa                                                                                                                                 <  1:160017/130‑1 (MQ=255)           aTCCCGCCCAGATTACGCAACCGTAACTGGCGAGCGATAGTCTGCGTCGCTTCAATATTGGTATTGAAAATGGTGTCGTCCAGATTGCGATGACCGACAAACGCTCCGGTATTGATGTCCACGGCGGTCa                                                                                                                                 >  2:160017/1‑130 (MQ=255)           aTCCCGCCCAGATTACGCAACCGTAACTGGCGAGCGATAGCCTGCGTCGCTTCAATATTGGTATTGAAAATGGTGTCGTCCAGATTGCGATGACCGACAAACGCTCCGGTATTGATGTCCACGGCGGTCATCGCTTCGGt                                                                                                                       >  1:374172/1‑140 (MQ=255)            tCCCGCCCAGATTACGCAACCGTAACTGGCGAGCGATAGCCTGCGTCGCTTCAATATTGGTATTGAAAATGGTGTCGTCCAGATTGCGATGACCGACAAACGCTCCGGTATTGATGTCCACGGCGGTCATCGCTTCGGTc                                                                                                                      >  2:528489/1‑140 (MQ=255)                 cccAGATTACGCAACCGTAACTGGCGAGCGATAGCCTGCGTCGCTTCAATATTGGTATTGAAAATGGTGTCGTCCAGATTGCGATGACCGACAAACGCTCCGGTATTGATGTCCACGGCGGTCATCGCTTCGGTCTGGTc                                                                                                                 >  1:33346/1‑140 (MQ=255)                 cccAGATTACGCAACCGTAACTGGCGAGCGATAGCCTGCGTCGCTTCAATATTGGTATTGAAAATGGTGTCGTCCAGATTGCGATGACCGACAAACGCTCCGGTATTGATATCCACGGCGGTCATCGCTTCGGTCTGGTc                                                                                                                 >  1:231528/1‑140 (MQ=255)                 cccAGATTACGCAACCGTAACTGGCGAGCGATAGCCTGCGTCGCTTCAATATTGGTATTGAAAATGGTGTCGTCCAGATTGCGATGACCGACAAACGCTCC‑GAATTGATGGCCATGGCGGTCATACCTTCGGTCTGGTCg                                                                                                                >  1:308700/1‑140 (MQ=255)                 cccAGATTACGCAACCGTAACTGGCGAGCGATAGCCTGCGTCGCTTCAATATTGGTATTGAAAATGGTGTCGTCCAGATTGCGATGACCGAAAAACGCTCGGGTATTGATGTCCACGGCGGTCATCGCTTCGGTCTGGTc                                                                                                                 >  2:214140/1‑140 (MQ=255)                 cccAGATTACGCAACAGTAACTGGCCAGCGATAGCCTGCGTCGCTTCAATATTGGTATTGAAAATGGAGTCGTCCAGATTGCGATTACCGACAAACGCTCCGGTATTGATGTCCACGGCGGACATCGCCTCGGTCTGGTc                                                                                                                 >  2:536970/1‑140 (MQ=255)                   cAGATTACGCAACCGTAACTGGCGGGCGAGAGCCTGCTTCGCTTCAATATTGGTATTGAAAATGGTGTCGTCCAGATTGCGATGACCGACAAACGCTCCGGTATTGATGTCCACGGCGGTCATCGCTTCGGTCTGGTCGa                                                                                                               <  2:492071/140‑1 (MQ=255)                     gATTACGAAACCGTAACTGGCGAGCGATAGCCTGCGTCGCTTCAATATTGGTATTGAAAATGGTGTCGTCCAGATTGCGATGACCGACAAACGCTCCGGTATTGATGTCCACGGCGGTCATCGCTTCGGTCTGGTCGATa                                                                                                             <  2:618485/140‑1 (MQ=255)                      aTTACGCAACCGTAACTGGCGAGCGATAGCCTGCGTCGCTTCAATATTGGTATTGAAAATGGTGTCGTCCAGATTGCGATGACCGACAAACGCTCCGGTATTGATGTCCACGGCGGTCATCGCTTCGGTCTGGCCGCTaa                                                                                                            >  1:522173/1‑140 (MQ=255)                               ccGTAACTGGCGAGCGATAGCCTGCGTCGCTTCAATATTGGTATTGAAAATGGTGTCGTCCAGATTGCGATGACCGACAAACGCTCCGGTATTGATGTCCACGGCGGTCATCGCTTCGGTCTGGTCGATAATGAGATAac                                                                                                   >  2:506977/1‑140 (MQ=255)                                cGTAACTGGCGAGCGATAGCCTGCGTCGCTTCAATATTGGTATTGAAAATGGTGTCGTCCAGATTGCGATGACCGACAAACGCTCCGGTATTGATGTCCACGGCGGTCATCGCTTCGGTCTGGTCGATAATGAGATAacc                                                                                                  >  1:30844/1‑140 (MQ=255)                                cGTAACTGGCGAGCGATAGCCTGCGTCGCTTCAATATTGGTATTGAAAATGGTGTCGTCCAGATTGCGATGACCGACAAACGCTCCGGTATTGATGTCCACGGCGGTCATCGCTTCGGTCTGGTCGATAATGAGATAacc                                                                                                  >  1:656396/1‑140 (MQ=255)                                  tAACTGGCGAGCGATAGCCTGCGTCGCTTCAATATTGGTATTGAAAATGGTGTCGTCCAGATTGCGATGACCGACAAACGCTCCGGTATTGATGTCCACGGCGGTCATCGCTTCGGTCTGGTCGATAATGAGATAaccac                                                                                                >  1:659210/1‑140 (MQ=255)                                  tAACTGGCGAGCGATAGCCTGCGTCGCTTCAATATTGGTATTGAAAATGGTGTCGTCCAGATTGCGATGACCGACAAACGCTCCGGTATTGATGTCCACGGCGGTCATCGCTTCGGTCTGGTAGATAATGAGATAaccac                                                                                                >  2:110846/1‑140 (MQ=255)                                       ggCGAGCGATAGCCTGCGTCGCTTCAATATTGGTATTGAAAATGGTGTCGTCCAGATTGCGATGACCGACAAACGCTCCGGTATTGATGTCCACGGCGGTCATCGCTTCGGTCTGGTCGATAATGAGATAACCACCGGAt                                                                                           <  1:528489/140‑1 (MQ=255)                                             cgaTAGCCTGCGTCGCTTCAATATTGGTATTGAAAATGGTGTCGTCCAGATTGCGATGACCGACAAACGCTCCGGTATTGATGTCCACGGCGGTCCTCCCCTCGGTCTGGGCGATAATGAAATAACCACCGGATTTCAGt                                                                                     >  2:120113/1‑140 (MQ=255)                                                tAGCCTGCGTCGCTTCAATATTGGTATTGAAAATGGTGTCGTCCAGATTGCGATGACCGACAAACGCTCCGGTATTGATGTCCACGGCGGTCCTCGCTTCGGTCTGGTCGATAATGAGATAACCACCGGATTTCAGTTCt                                                                                  >  1:644601/1‑140 (MQ=255)                                                       cGTCGCTTCAATATTGGTATTGAAAATGGTGTCGTCCAGATTGCGATGACCGACAAACGCTCCGGTATTGATGTCCACGGCGGTCATCGCTTCGGTCTGGTCGATAATGAGATAACCACCGGATTTCAGTTCTACTTTGc                                                                           >  2:372200/1‑140 (MQ=255)                                                       cGTCGCTTCAATATTGGTATTGAAAATGGTGTCGTCCAGATTGCGATGACCGACAAACGCTCCGGTATTGATGTCCACGGCGGTCATCGCTTCGGTCTGGTCGATAATGAGATAACCACCGGATTTCAGTTCTACTTTGc                                                                           >  1:421530/1‑140 (MQ=255)                                                               cAATATTGGTATTGAAAATGGTGTCGTCCAGATTGCGATGACCGACAAACGCTCCGGTATTGATGTCCACGGCGGTCATCGCTTCGGTCTGGTCGATAATGAGATAACCACCGGATTTCAGTTCTACTTTGCGTTCCAgc                                                                   <  2:374172/140‑1 (MQ=255)                                                                         aTTGAAAATGGTGTCGTCCAGATTGCGATGACCGACAAACGCTCCGGTATTGATGTCCACGGCGGTCATCGCTTCGGTCTGGTCGATAATGAGATAACCACCGGATTTCAGTTCTACTTTGCGTTCCAGCGCTCGCTGGa                                                         <  1:434699/140‑1 (MQ=255)                                                                          ttGAAAATGGTGTCGTCCAGATTGCGATGACCGACAAACGCTCCGGTATTGATGTCCACGGCGGTCATCGCTTCGGTCTGGTCGATAATGAGATAACCACCGGAGTTCCGTTCTACTCTGCGTTCCAGCGCTCGCTGGAt                                                        >  2:261514/1‑140 (MQ=255)                                                                                            aGATTGCGATGACCGACAAACGCTCCGGTATTGATGTCCACGGCGGTCATCGCTTCGGTCTGGTCGATAATGAGATAACCACCGGATTTCAGTTCTACTTTGCGTTCCAGCGCTCGCTGGATTTCGTTTTCGACATCaaa                                      <  2:659210/140‑1 (MQ=255)                                                                                                       aCCGACAAACGCTCCGGTATTGATGGCCACGGCGGTCATCGCTTCGGTCTGGTCGATAATGAGATAACCACCGGATTTCAGTTCTACTTTGCGTTCCAGCGCTCGCTGGATTTCGTTTTCGACATCAAAGAGATCGaaaa                           >  2:461297/1‑140 (MQ=255)                                                                                                              aaCGCTCCGGTATTGATGTCCACGGCGGTCATCGCTTCGGTCTGGTCGATAATGAGATAACCACCGGATTTCAGTTCTACTTTGCGTTCCAGCGCTCGCTGGATTTCGTTTTCGACATCAAAGAGATCGAAAATCGGCTg                    <  1:506977/140‑1 (MQ=255)                                                                                                              aaCGCTCCGGTATTGATGTCCACGGCGGTCATCGCTTCGGTCTGGTCGATAATGAGATAACCACCGGATTTCAGTTCTACTTTGCGTTCCAGCGCTCGCTGGATTTCGTTTTCGACATCAAAGAGATCGAAAATCGGCTg                    <  1:381957/140‑1 (MQ=255)                                                                                                                cGCTCCGGTATTGATGTCCACGGCGGTCAGCGCTTCGGTCTGGTCGATAATGAGATAACCACCGGATTTCAGTTCTACTTTGCGTTCCAGCGCTCGCTGGATTTCGTTTTCGACATCAAAGAGATCGAAAATCGGCTGGc                  <  1:261409/140‑1 (MQ=255)                                                                                                                  cTCCGGTATTGATGTCCACGGCGGTCATCGCTTCGGTCTGGTCGATAATGAGATAACCACCGGATTTCAGTTCTACTTTGCGTTCCAGCGCTCGCTGGATTTTGTTTTCGACATTAAAGAGATCGAAAATCGGCTGGCGt                >  1:271577/1‑140 (MQ=255)                                                                                                                  cTCCGGTATTGATGTCCACGGCAGTGATCGCTTCGGTATGGTCGATAATGAGATAACCACCGGATTTCAGTTCTACTTTGCGTTCCAGCGCTCGCTGGATTTCGTTTTCGACATCAAAGAGATCGAAAATCGGCTGGCGt                <  1:214140/140‑1 (MQ=255)                                                                                                                   tCCGGTATTGATGTCCACGGCGGTCATCGCTTCGGTCTGGTCGATAATGAGATAACCACCGGATTTCAGTTCTCCTTTGCGTTCCAGCGCTCGCTGGATTTCGTTTTCGACATCAAAGAGATCGAAAATCGGCTGGCGTc               >  2:379393/1‑140 (MQ=255)                                                                                                                     cGGTATTGATGTCCACGGCGGTCATCGCTTCGGTCTGGTCGATAATGAGATAACCACCGGATTTCAGTTCTACTTTGCGTTCCAGCGCTCGCTGGATTTCGTTTTCGACATCAAAGAGATCGAAAATCGGCTGGCGTCCt             >  2:296095/1‑140 (MQ=255)                                                                                                                     cGGTATTGATGTCCACGCCGGTGATCGCTTCGGTCTGGTCGATAATGAAATAACCACCGGATTTCAGTTCTACTTTGCGTTCCAGCGCTCGCTGGATTTCGTTTTCGACATCAAAGAGATCGAAAATCGGCTGGCGTCCt             <  2:271577/140‑1 (MQ=255)                                                                                                                                tCCACGGCGGTCATCGCTTCGGTCTGGTCGATAATGAGATAACCACCGGATTTCAGTTCTACTTTGCGTTCCAGCGCTCGCTGGATTTCGTTTTCGACATCAAAGAGATCGAAAATCGGCTGGCGTCCTGTGTAATGCTc  <  2:157012/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                     AATAATGATAATCCCGCCCAGATTACGCAACCGTAACTGGCGAGCGATAGCCTGCGTCGCTTCAATATTGGTATTGAAAATGGTGTCGTCCAGATTGCGATGACCGACAAACGCTCCGGTATTGATGTCCACGGTGGTCATCGCTTCGGTCTGGTCGATAATGAGATAACCACCGGATTTCAGTTCTACTTTGCGTTCCAGCGCTCGCTGGATTTCGTTTTCGACATCAAAGAGATCGAAAATCGGCTGGCGTCCTGTGTAATGCTC  >  NC_000913/3396758‑3397024

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑