Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,474,354	T→C	I67V (ATC→GTC)	rpsL ←	30S ribosomal subunit protein S12

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,474,354	0	T	C	100.0%	221.5 / NA	61	I67V (ATC→GTC)	rpsL	30S ribosomal subunit protein S12
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (24/37);  total (24/37)

CGGTCTTTAACGCCGGAGCAGTCAAGCGCACCACGTACGGTGTGGTAACGAACACCCGGGAGGTCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTTTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTC  >  NC_000913/3474224‑3474480                                                                                                                                   |                                                                                                                               cGGTCTTTAACGCGGGAGCAGTTAAGCGCACCACGAACGTTGTGGTAACGAACACCCGGGAGGTCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGACGTAGGAAGt                                                                                                                       <  2:275715/140‑1 (MQ=255) cGGTCTTTAACGCCGGAGCAGTCAAGCGCACCACGTACGGTGTGGTAACGAACACCCGGGAGGTCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGACGTAGGAAGt                                                                                                                       <  1:521069/140‑1 (MQ=255)  ggTCTTTAACGCCGGAGCAGTCAAGCGCACCACGTACGGTGTGGTAACGAACACCCGGGAGGTCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGACGTAGGAAGTc                                                                                                                      >  1:100653/1‑140 (MQ=255)  ggTCTTTAACGCCGGAGCAGTCAAGCGCACCACGTACGGTGTGGTAACGAACACCCGGGAGGTCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGACGTAGGAAGTc                                                                                                                      >  1:613440/1‑140 (MQ=255)       ttAACGCCGGAGCAGTCAAGCGCACCACGTACGGTGTGGTAACGAACACCCGGGAGGTCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGACGTAGGAAGTCACTTc                                                                                                                 >  1:126627/1‑140 (MQ=255)       ttAACGCCGGAGCAGTCAAGCGCACCACGTACGGTGTGGTAACGAACACCCGGGAGGTCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGACGTAGGAAGTCACTTc                                                                                                                 >  1:408898/1‑140 (MQ=255)              cGGAGCAGTCAAGCGCACCACGTACGGTGTGGTAACGAACACCCGGGAGGTCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGACGTAGGAAGTCACTTCg                                                                                                                >  2:17595/1‑134 (MQ=255)              cGGAGCAGTCAAGCGCACCACGTACGGTGTGGTAACGAACACCCGGGAGGTCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGACGTAGGAAGTCACTTCg                                                                                                                <  1:17595/134‑1 (MQ=255)              cGGAGCAGTCAAGCGCACCACGTACGGTGTGGTAACGAACACCCGGGAGGTCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGACGTAGGAAGTCACTTCGAAACCg                                                                                                          <  2:613440/140‑1 (MQ=255)              cGGAGCAGTCAAGCGCACCACGTACGGTGTGGTAACGAACACCCGGGAGGTCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGACGAAGGAAGTCACTTCGAAACCg                                                                                                          >  2:33953/1‑140 (MQ=255)                          gcgcACCACGTACGGTGCGGTACCGAACACCGGGGAGGTTTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGACGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGa                                                                                              <  2:211867/140‑1 (MQ=255)                          gcgcACCACGTACGGCGTGGTAACGCACACCCGGGAGGTCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGACGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGa                                                                                              <  1:224524/140‑1 (MQ=255)                           cgcACCACGTACGGTGTGGTAACGAACACCCGGGAGGTCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGACGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGaa                                                                                             <  1:33953/140‑1 (MQ=255)                           cgcACCACGTACGGTGTCGTAAGGAACACCCTGGAGGTCTTTAACACGGCCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGACGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGaa                                                                                             <  2:271934/140‑1 (MQ=255)                             caccacGTACGGTGTCGTAACGGACTCCCCGGAGGTCGTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGACGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAaca                                                                                           <  1:306645/140‑1 (MQ=255)                               ccacGTACGGTGTGGTAACGAACACCCGGGAGGTCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGACGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACg                                                                                         <  2:100653/140‑1 (MQ=255)                                 acGTACGGTGTGGTAACGAACACCCGGGAGGTCTTTAACACGATCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGACGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGc                                                                                       <  1:268659/140‑1 (MQ=255)                                 acGTACGGTGTGGTAACGAACACCCGGGAGGTCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGACGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGc                                                                                       <  1:377925/140‑1 (MQ=255)                                 acGTACGGTGTGGTAACGAACACCCGGGAGGTCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGACGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGc                                                                                       <  1:509690/140‑1 (MQ=255)                                 acGTACGGTGTGGTAACGAACACCCGGGAGGTCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGACGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGc                                                                                       <  1:373356/140‑1 (MQ=255)                                          gtgGTAACGAACACCCGGGAGGTCTTTAACACGACCGCTACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGACGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTAc                                                                              >  2:77283/1‑140 (MQ=255)                                          gtgGTAACGAACACCCGGGAGGTCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGACGTAGGAAGTCGCTTAGAAGCCGTTAGTAAGACGAACACGGCATACTTTAc                                                                              >  2:257559/1‑140 (MQ=255)                                            ggTAACGAACACCCGGGAGGTCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGACGTATGAAGTCACATCGAAACAGTTAGTCAAACGAGCACGGCATACTTTACGc                                                                            >  2:291487/1‑140 (MQ=255)                                            ggTAACGAACACCCGGGAGGTCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGACGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGc                                                                            >  1:612632/1‑140 (MQ=255)                                                aCGAACACCCGGGAGGTCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGACGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAgcg                                                                        <  2:465717/140‑1 (MQ=255)                                                aCGAACACCCGGGAGGTCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGACGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAgcg                                                                        <  1:31642/140‑1 (MQ=255)                                                 cGAACACCCGGGAGGTCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGACGTAGGAAGCCACTTCGAAACCGTTAATCAGACGACCACGACATACTTTACGCAgcgc                                                                       >  2:282101/1‑140 (MQ=255)                                                   aaCACCCGGGAGGTCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGACGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCgg                                                                     >  2:149318/1‑140 (MQ=255)                                                       cccGGGAGGTCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGACGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGtt                                                                 >  2:57789/1‑140 (MQ=255)                                                        ccGGGAGGTCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGACGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTc                                                                <  1:464330/140‑1 (MQ=255)                                                                  tttAACACGACCGCCACGGATCAGGATCACGGAGTGCTTCTGCAGGTTGTGACCTTCACCACCGACGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTTTAg                                                      <  2:21288/140‑1 (MQ=255)                                                                  tttAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGACGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTTTAg                                                      >  2:74297/1‑140 (MQ=255)                                                                  tttAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGACGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTTTAg                                                      >  2:539355/1‑140 (MQ=255)                                                                  tttAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGACGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTTTAg                                                      <  2:112336/140‑1 (MQ=255)                                                                  tttAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGACGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTTTAg                                                      <  1:572889/140‑1 (MQ=255)                                                                    tAACACGACCGCCACGGATCAGTATTACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGACGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTTTAGGa                                                    <  1:257559/140‑1 (MQ=255)                                                                     aaCACGACCGCTACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGACGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTTTAGGAg                                                   <  1:77283/140‑1 (MQ=255)                                                                     aaCACGACCGCCACGGATCAGGTTCACGGAGCGCTACTGCAGGTTGTGTCCTTCACCACCGACGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTTTAGGAg                                                   <  2:218488/140‑1 (MQ=255)                                                                     aaCACGACCGCCACGGATCAGGTTCACGGAGAGCTCCTGAAGGTTGTGACCTTCACCACCGACGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTTTAGGAg                                                   <  2:201644/140‑1 (MQ=255)                                                                      acacGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGACGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTTTAGGAGt                                                  <  2:481660/140‑1 (MQ=255)                                                                       cacGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGACGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTTTAGGAGTg                                                 <  1:603835/140‑1 (MQ=255)                                                                       cacGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGACGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTTTAGGAGTg                                                 <  1:539355/140‑1 (MQ=255)                                                                        acGACCGCCACGGATGAGGATCCCGTAGTTCTCCTGCGGGTTGTGACCTTCACCACCGACGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTTTAGGAGTgg                                                <  1:282101/140‑1 (MQ=255)                                                                         cGACCGCCACGGATCAGCATCACGGAGTGCTCCTGGAGGCTGTGACCTTCACCACCGACGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTTTAGGAGTGgt                                               <  2:255090/140‑1 (MQ=255)                                                                          gACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGACGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTTTAGGAGTGgta                                              <  2:126627/140‑1 (MQ=255)                                                                          gACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGACGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTTTAGGAGTGgta                                              <  1:132144/140‑1 (MQ=255)                                                                          gACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGACGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTTTAGGAGTGgta                                              <  2:396189/140‑1 (MQ=255)                                                                          gACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGACGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTTTAGGAGTGgta                                              >  1:661203/1‑140 (MQ=255)                                                                               ccACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGACGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTTTAGGAGTGGTAGtata                                         <  2:408898/140‑1 (MQ=255)                                                                               ccACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGACGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTTTAGGAGTGGTAGtata                                         <  1:29864/140‑1 (MQ=255)                                                                                   ggatcaggatcaCGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGACGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTTTAGGAGTGGTAGTATATaca                                     <  2:78047/140‑1 (MQ=255)                                                                                               cGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGACGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTTTAGGAGTGGTAGTATATACACGAGTACATACg                         >  2:64248/1‑140 (MQ=255)                                                                                                  aGTGCTCCTGCAGGTTGTGACCTTCACCACCGACGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTTTAGGAGTGGTAGTATATACACGAGTACATACGCCa                      >  1:599430/1‑140 (MQ=255)                                                                                                    tGCTCCTGCAGGTTGTGACCTTCACCACCGACGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTTTAGGAGTGGTAGTATATACACGAGTACATACGCCACg                    >  2:80148/1‑140 (MQ=255)                                                                                                      cTCCTGCAGGTTGTGACCTTCACCACCGACGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTTTAGGAGTGGTAGTATATACACGAGTACATACGCCACGtt                  >  2:507016/1‑140 (MQ=255)                                                                                                              ggTTGTGACCTTCACCACCGACGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTTTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCggg          >  1:653272/1‑140 (MQ=255)                                                                                                               gTTGTGACCTTCACCACCGACGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTTTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGc         >  1:154552/1‑140 (MQ=255)                                                                                                                 tgtgACCTTCACCACCGACGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTTTGGGAGTGGTGGTAGATAAACGAGTACTTACGTCACGTTTTTGCGGGCAt       >  2:257862/1‑140 (MQ=255)                                                                                                                 tgtgACCTTCACCACCGACGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTTTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCAt       <  1:83980/140‑1 (MQ=255)                                                                                                                 tgtgACCTTCACCACCGACGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTTTAGGAGTGGTAGTATATACACGAGAACATACGCCACGTTTTTGCGGGCAt       >  2:373236/1‑140 (MQ=255)                                                                                                                      ccTTCACCACCGACGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTTTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTc  <  2:612632/140‑1 (MQ=255)                                                                                                                                   |                                                                                                                               CGGTCTTTAACGCCGGAGCAGTCAAGCGCACCACGTACGGTGTGGTAACGAACACCCGGGAGGTCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTTTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTC  >  NC_000913/3474224‑3474480

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑